• Journal of Korean Society on Water Environment
• /
• v.29 no.4
• /
• pp.531-539
• /
• 2013

In order to apply a water quality indicator as a certain target pollutant, the indicator should have the representability of an index for the management of Total Maximum Daily Loads (TMDLs). Scientific groundwork and treatment technologies also should be provided for the application of the indicator. This study analyzed the applicability of major water quality indicators for target pollutants. The results showed that four water quality indicators of TOC, T-N, SS & T-Coli can be considered as target pollutants besides existing BOD and T-P. These indicators can be applied with the preparations such as essential basic data, pollution load estimation framework as well as legal criteria setting when the need is raised. As the importance of TOC, which is considered as a replacement of BOD, is emphasized, technical and institutional terms are being prepared in order to determine TOC as another target pollutant in the near future. It could be considered to combine TOC with BOD for the time being during the $3^{rd}$ stage of TMDLs in view of the present conditions for the management of TOC. TOC can be the most effective index for the organic matter and help to be managed more systematically in the waterbodies where rivers and lakes are mutually connected.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.41 no.2
• /
• pp.44-54
• /
• 1999

Agricultural water quality standards were reviewed through rice culture using treated sewage irrigation . The seqage from school building of Konkuk University was treated by a constructed wetland system, and theeffluent of the systeml was irrigated for rice culutre after nutrient concentration adjusted by dilution. Average concentration of COD, SS, T-N and T-P in irrigated water was 22.3mg/$\ell$, 6.5mg/$\ell$, 25.8 mg/$\ell$and 2.2mg/$\ell$, respectively. Treatment include irrigation of adjusted effluent with conventional fertilization (TWCF), adjusted effluent with no fertilization (TWNF). and effluent of the wetland system as it was with no fertilization (SWNF). These treatment plots were compared with control plot irrigated by tap water with conventional fertilization (CONTROL). Other environmentals for rice culture were identical for all the plots. Among them, TWCF showed the best growth rate and the highest yield, and constituents in the harvested rice showed not much difference among them. Which implies that irrigation with relatively high nutrient concentration compared to the current water quality standards may cause no adverse effect on rice culture and could be even beneficial . Although T-N for this study was 25 times greater than the current standards, rice culture wasnot adversely affected by irrigatino water quality and even beeter results were observed than the CONTROL. It could be mistakenly that clean irrigation water produces better agricultural product, however, it is not necessarily true. Irrigation water with moderate nutrient concentration can enhance the plant growth, and better result might be expected. Therefore, peer review and modification if necessary are needed to the current agricultural water quality standards, especially for the nutrient components.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.5
• /
• pp.662-667
• /
• 2020

Epidermal growth factor receptor (EGFR) mutations are not only genetic markers for diagnosis but also biomarkers of clinical-response against tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC). Among the EGFR mutations, the in-frame deletion mutation in EGFR exon 19 kinase domain (EGFR exon 19-del) is the most frequent mutation, accounting for about 45% of EGFR mutations in NSCLCs. Development of sensitive method for detecting the EGFR mutation is highly required to make a better screening for drug-response in the treatment of NSCLC patients. Here, we developed a fluorometric tandem gene amplification assay for sensitive detection of low-abundance EGFR exon 19-del mutant genomic DNA. The method consists of pre-amplification with PCR, thermal cycling of ligation by Taq ligase, and subsequent rolling circle amplification (RCA). PCR-amplified DNA from genomic DNA samples was used as splint DNA to conjugate both ends of linear padlock DNA, generating circular padlock DNA template for RCA. Long stretches of ssDNA harboring multiple copies of G-quadruplex structure was generated in RCA and detected by thioflavin T (ThT) fluorescence, which is specifically intercalated into the G-quadruplex, emitting strong fluorescence. Sensitivity of tandem gene amplification assay for detection of the EGFR exon 19-del from gDNA was as low as 3.6 pg, and mutant gDNA present in the pooled normal plasma was readily detected as low as 1% fraction. Hence, fluorometric detection of low-abundance EGFR exon 19 deletion mutation using tandem gene amplification may be applicable to clinical diagnosis of NSCLC patients with appropriate TKI treatment.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.13 no.3
• /
• pp.46-62
• /
• 2010

This study was conducted to understand the water and soil properties to propose the promotion of vegetation environment at farm pond depression. We selected 8 palustrine wetlands from agricultural area after consideration of human interference, surround land use, and size of area. Water quality analysis showed that the average SS, T-N, T-P were over the limit of agricultural water quality standard level at some sites. The cause for deterioration of water quality is supposed by the long-term stagnation of water in palustrine wetland. The recommended measures to improve water quality are as follows; improving water circulation by connecting with nearby natural water, preventing oxygen depletion by dredging deposit, lowering down T-N and T-P by removing autumn plants, preventing inflow of phosphorus in fertilizer ingredients which is the main cause for high T-P. The soil contamination of the surveyed area was about the same level of average heavy metal contents in soils from 2,010 paddy fields in Korea, which was much lower than soil contamination standards. As for soil texture, sand content was 40~90% and clay content was less than 20%. The content of silt and clay in soil from community of floating-leaved:submerged hydrophytes and community of emergent hydrophytes was higher that of soil from community of hygrophytes, and the content of sand in soil from community of hygrophytes was 10% higher than underwater soil. In terms of bulk density, the average was 0.24~0.96g/$cm^3$, which was quite low, because of high content of peat and organic matter in soil of the surveyed area. As for the average content of organic matter, community of floating-leaved:submerged hydrophytes was 18.25g/kg, community of emergent hydrophytes was 16.88g/kg, and community of hydrophytes was 25.63g/kg. The range of content of T-N in soil of community of floating-leaved；submerged hydrophytes was 0.022~0.307%, and that of community of emergent hydrophytes was 0.029~0.681% and that of community of hydrophytes was 0.088~0.325%. Apart from three sites in the surveyed area, most parts were over the standards or below the standard. After this study, we will conduct and discuss the relationship between vegetation characteristics and environments, which will be used of the best practical management and restoration of wetland.

• Journal of agriculture & life science
• /
• v.46 no.3
• /
• pp.129-141
• /
• 2012

The purpose of this study was to monitor the stream flow of rural streams for investigating the status of stream depletion located downstream of irrigation reservoir. Bonghyun and Hai reservoirs area, located in Hai-myeon, the city of Gosung, Gyeongsangnam Province, were selected for study watersheds and streams. Stream flow monitoring was conducted 7 times from March to September, 2011. Stream flow was measured for 8 stations downstream from two reservoirs. The stream depletion was found in most of the downstream of reservoirs for the non-irrigation period and even in the irrigation period when there were a lot of antecedent precipitation. The correlation analysis for water quality data indicated that the correlation between BOD and T-N was highest for the reservoirs. The correlation between BOD, T-N, and turbidity was high for Hai reservoir and Bonghyeon reservoir. Continuous monitoring for rural streams located in downstream of reservoirs are required to quantify the status of stream flow depletion and determine the amount of environmental flows.

• Magazine of the Korea Concrete Institute
• /
• v.7 no.3
• /
• pp.175-186
• /
• 1995

The determination of the stress intensity factors is investigated by sur-face integral method around the crack tip of the nlass~vc: concrete structure. The surface integral met hod is naturally derived from the standal-ci path integral J. Howevcr. In the J integral method, pressure in the crack-face and body forces can not be considered, while this theory has advantage of ccmsidering many kind of forces, so t.his theory will be useful in investigating more accurate strt:ss states around crack tip. Furthermore. t h~s rrlethod can elerninate unntussary process of using singular elements and fine mesh around crack tip which is used 11; modelling the singularity around crack tip. A computer program for determming $K_I$, $K_{II}$ is tfcvulopcd by applying this theory. $K_I$, $K_{II}$ values usmg X noded isoparametric elements which was proved and variation of the stress intensity factor was investigated by application of darn structures.

• Journal of Korean Society of Water and Wastewater
• /
• v.19 no.1
• /
• pp.25-30
• /
• 2005

In order to decrease the high costs of membrane process, we have tried to develop two alternatives to membrane; a cartridge type filter and a metal membrane were tested for the high permeation flux with low cost and low energy. This research mainly focused on three points; 1) operation with high permeation flux by using of a cartridge type filter and a metal membrane, 2) removals of the filterable organic materials (FOC) by pretreatments for the membrane fouling control, and 3) advanced wastewater treatment by SMBR process with intermittent aeration and high MLSS. An Intermittently aerated membrane bioreactor using a submerged micro filter (cartridge type) was applied in laboratory scale for the advanced wastewater treatment. To minimize membrane fouling, intermittent aeration was applied inside of the filter with $3.0kg_f/cm^2$. The experiments was conducted for 6 months with three different HRTs (8, 10, 12 hr) and high MLSS of 6,000 and 10,000mg/L. The filtration process could be operated up to 50 days with permeation flux of 500LMH. Regardless of the operating conditions, more than 95% of COD, BOD and SS were removed. Fast and complete nitrification was accomplished, and denitrification was appeared to be the rate-limiting step. More than 75% T-N could be removed due to the endogenous denitrification. T-P removal efficiency was increased to 80% under the condition of MLSS 10,000mg/L.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2009.05a
• /
• pp.1817-1821
• /
• 2009

최근 전자제품 사용의 증가로 인해 버려지는 전자제품의 양도 함께 증가하고 있으며 이렇게 늘어난 전자폐기물이 부적절한 방식으로 처리될 경우 우리에게 악영향을 미치는 위험물질이 될 수 있다. 본 연구에서는 이러한 전자폐기물 중 하나인 폐 LCD 유리를 이용한 수질정화용 여재를 이용하여 전자폐기물에 의한 오염을 줄이고, 동시에 강우시 표면 유출로 인하여 발생되는 비점오염을 제어하고자 하였다. 본 연구에서 이용한 수처리 여재는 기존 수처리 여재의 단점을 보완하는 대체 여재 개발이라는 목적에 적합할 것으로 판단된다. 이에 본 연구에서 발포기술이 적용된 폐 LCD 유리 여재의 비점오염에 의한 수질정화능력평가를 하기 위해 실험 장치를 구성하여 실험하였다. 반응조는 직경 0.10 m, 높이 0.75 m 크기(총용량= 5.89 $\ell$)로 두께 10mm의 투명 아크릴판으로 3조를 제작하였다. 유입수는 미량 펌프를 이용하여 유입하였다. 각 반응조의 체류시간에 의한 수질정화능력을 평가하였다. 연구 결과, 오염물질에 대한 제거효율 범위는 SS $64.1%\sim88.2%$, BOD $29.8\sim52.4%$, COD $56.8\sim66.1%$, T-N $39.4\sim\;52.3%$, T-P $14.1\sim36.8%$로 나타났다. 체류시간이 가장 길었던 반응조의 경우, 대부분의 측정항목에서 높은 처리 효율을 볼 수 있었다. 초기에 처리 효율이 낮은 것은 미생물이 부착되지 않았기 때문이라고 판단되며, 실험의 중반과 후반에서 미생물이 부착이 되는 시점부터 높은 처리효율을 보이는 것으로 나타났다.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.54 no.2
• /
• pp.27-35
• /
• 2012

Various Best Management Practices (BMPs) have been suggested to reduce Nonpoint source pollutant loads from agricultural fields. However, very little research regarding water quality improvement with sediment trap has been performed in Korea. Thus, effects of sediment trap were investigated in this study. Three sediment traps were installed at the edge of six plots and flow and water quality of inflow and outflow were monitored and analyzed. It was found that approximately 64.1 % of flow reduction was observed. In addition, pollutant concentration of outflow was reduced by 39.0 % for $BOD_5$. For SS, $COD_{Mn}$, DOC, T-N, T-P, approximately 62.1 %, 43.4 %, 43.5 %, 40.0 %, and 41.2 % reduction were observed, respectively. Over 80 % and 90 % of pollutant loads were reduced from sediment trap #2 and #3 because of less outflow from plots covered with rice straw/straw mat. In case of intensive rainfall events occurred from July 26~29, 2011, over 60 % of pollutant and 88.9 % of sediment reduction were observed from sediment trap #3. As shown in this study, small sediment traps could play important roles in reducing pollutant loads from agricultural fields. If proper management practices, such as rice straw/straw mat, are used to protect surface from rainfall impacts and rill formation, much pollutant reduction could be expected.

• Journal of Korean Society on Water Environment
• /
• v.34 no.3
• /
• pp.285-292
• /
• 2018

This study was performed in order to identify the red tide-causing organism and to understand the characteristics of the water quality during the winter of 2015 and 2016 in the Oncheon stream, a tidal river in Busan, where red tide often occurs in the wintertime. Two sites were selected on the stream and the surface water was sampled a total of 28 times during the experimental period. Twelve water quality characteristics, including water temperature, pH, DO, COD, total-N (T-N), total-P (T-P), and salinity were analyzed in order to test water quality. The cell numbers of cryptomonads were counted directly by microscopic observation. The nucleotide sequences of the partial 28S rRNA gene and psbA gene from metagenomic DNA, derived from each sampling site, were analyzed. According to the results, the alga most responsible for the bloom was identified as Teleaulax OC1 sp., which belongs to the cryptomonads. Three items of chl-a, pH, and DO were positively correlated with the cell numbers of the cryptomonads counted at the upper stream of the tidal area (St 1) while eight items of chl-a, TOC, BOD, total-N, COD, SS, pH, and DO were positively correlated with the cells located at the junction between the stream and Su-young river (St 2) in the order.