• Plant Resources
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• v.7 no.1
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• pp.39-46
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• 2004

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Journal of the Korea Fashion and Costume Design Association
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• v.15 no.1
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• pp.91-109
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• 2013

The main objective of this research was to understand the latest trends of natural print design through the quantitative & qualitative analysis of fashion appeared in contemporary female collections. The research criteria was defined as 3 seasons from 2011 S/S to 2012 S/S. Data collection of 726 was done through review of 'pr$\hat{e}$t-$\grave{a}$-porter Collections' of three major fashion cities; Paris, Milan and NY. Statistical analysis of frequency with chi-square test was conducted. Also qualitative interpretation of natural print design' characteristics was completed. The main findings were as follows.; The average occurrence rate of natural print design from 2011SS to 2012 SS in three collections were 6.4% in Milan 6.4%, 5.5% in Paris and 6.8% in N.Y. The five source types of natural prints in contemporary women's fashion collections were identified and the order of their appearance were as follows: flowers, plants, animals, insects & marine organisms and compound one. The plant prints were expressed by stylized or realistic touch. Flower patterns showed more variables than plants, however, there were no big difference in their image and major characteristics. The animal prints demonstrated two aspects. First one used typical animal print of fur or skin, but the other one draw the animal figure like paintings. The compound source type presented the most interesting and fresh pattern design ideas. In the insects & marine organisms, mainly butterfly and seashell & starfish, etc. appeared as real shapes or sometimes were stylized.

• Journal of Environmental Impact Assessment
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• v.23 no.3
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• pp.187-196
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• 2014

The objective of this study is to evaluate and analyze Osu stream watershed water environment system. The data were collected from January 2009 to December 2011 including water temperature, pH, DO, EC, BOD, COD, TOC, SS, T-N, T-P and discharge. The data were used for principle component analysis and factor analysis. The results are as followes. The primary factors obtained from both the principal component analysis and the factor analysis were BOD, COD, TOC, SS and T-P. Once principal component analysis and factor analysis have been performed with the collected data and then the results will be applied to both simple regression model and multiple regression model. The regression model was developed into case 1 using concentrations of water quality parameters and case 2 using delivery loads. The value of the coefficient of determination on case 1 fell between 0.629 and 0.866; this was lower than case 2 value which fell between 0.946 and 0.998. Therefore, case 2 model would be a reliable choice.The coefficient of determination between the estimated figure using data which was developed to the regression model in 2012 and the actual measurement value was over 0.6, overall. It can be safely deduced that the correlation value between the two findings was high. The same model can be applied to get TOC concentrations in future.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.3
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• pp.121-127
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• 2015

The objective of this study is to suggest the water quality criteria of total nitrogen in order to efficiently manage the nutrient pollution in the stream. For this, correlations between water quality parameters were examined using the water quality data collected from the water quality monitoring network in the four rivers between 2003 and 2012. T-N showed positive correlations with T-P (0.636), COD (0.577), BOD (0.574), TOC (0.440), and SS (0.367). The statistical analysis including percentile analysis for the T-N and T-P concentrations was utilized to develop the water quality criteria of T-N. The feasibility of the suggested water quality criteria was evaluated by calculating the achievement rate to water quality target at the representative points in mid-watershed, then the draft water quality standard of T-N was suggested. The suggested water quality standard of T-N in the stream may be used to efficiently control the nutrient pollution in the public water body.

• Korean Journal of Environmental Agriculture
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• v.20 no.4
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• pp.277-283
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• 2001

This paper presents treatment efficiency and plant growth of a surface-flow constructed wetland system (30 meter in length and 10 meter in width) over one year after its establishment on a floodplain of a stream. Cattails (Typha angustiflora) grown on pots were transplanted on one half of its area from inlets and reeds (Phragmites australis) on another half from an outlet. Effluent discharged from a secondary-level treatment plant was funneled into the system. The stems of cattails and reeds emerging in April 2001 grew up to 165.9 cm and 95.3 cm, respectively until July 2001. The number of stems of cattails arid reeds in July 2001 increased by 65% and 100%, respectively, compared with that just after their planting. The growth of cattails was better than that of reeds during study period. The removal rates for SS, $BOD_5$, T-N and T-P was 33%, 43%, 31%, and 51%, respectively. The system was inundated seven times by storms over the monitoring period, which disturbed its environment and led to its lower treatment rates. The increase of SS concentration in effluent after inundation of the system was attributed to the falls of soil particles onto its water surface, which had been attached to the emergent plants by floods. Purification rates for T-N were relatively low for the period of late fall through winter until early spring due to lower water temperature which may have retarded microbial nitrification and denitrification mechanisms. Reduction in T-P concentration during fall and winter was relatively higher than that during summer and spring, which may have resulted from no system perturbations by floods and heavy storms during fall and winter.

• Proceedings of the KAIS Fall Conference
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• 2007.05a
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• pp.116-118
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• 2007

비강우시와 강우시 학장천의 수질은 BOD, COD, T-N, T-P, SS에 대해 각각 $11.0{\sim}31.3mg/L$, $15.6{\sim}31.4mg/L$, $5.762{\sim}15.937mg/L$, $0.918{\sim}1.291mg/L$, $18.1{\sim}31.41mg/L$와 $27.2{\sim}65.11mg/L$, $32.1{\sim}73.2rng/L$, $13.409{\sim}18.051mg/L$, $1.263{\sim}2.282mg/L$, $66.0{\sim}417.9mg/L$로 나타나 학장천은 전형적인 생활하수의 특성을 지녔으며, 강우시 발생되는 초기월류수에 다량의 비점오염물질이 포함됨을 알 수 있었다. 강우시 발생된 월류수의 유량을 측정한 결과 학장천의 유출계수는 $0.61{\sim}0.83$의 범위였고, 초기세척효과는 SS>T-N>COD>BOD>T-P>1 의 순서로 그 정도가 크게 나타났다. 학장천 초기월류수의 유량가중평균농도를 산정한 결과 대부분의 항목에서 청천시의 수질농도보다 높게 나타나 강우에 의한 월류수의 비점오염물질의 유출이 심각함을 알 수 있다.

• Journal of Korean Society on Water Environment
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• v.29 no.5
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• pp.648-655
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• 2013

Nonpoint source pollution characteristics of recreation park was investigated. Runoff ratio of recreation park ranged 23-57%, which was lower than other urban area since impervious area was less than 37%. The average BOD, COD, TOC, SS, T-N, T-P, were 14.09, 32.86, 12.19, 121.51, 7.78 and 0.72 mg/L, respectively. First flush of recreation park was analyzed by normalized cumulative load - volume curve and mass first flush ratio(MFFn), MFF10 for BOD, COD, SS, T-P, T-N, TOC were 2.90, 1.59, 2.15, 2.74, 2.60, and 1.59, respectively. Observed data showed that 62% of pollutant could be removed by storaging 5 mm rainfall-runoff and even 3 mm depth could store up to 50% of pollutant in runoff.

• Magazine of the Korean Society of Agricultural Engineers
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• v.39 no.5
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• pp.97-108
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• 1997

Natural wastewater treatment systems using the constructed wetland system were evaluated for the wastewater from the industrial complex in rural areas. For the treatment of wastewater from the industrial complex in rural area, a pilot plant of the constructed wetland system was installed at Baeksuk agri-industrial complex in ChunahnCity, Chunchungnam-Do. The experiment with this pilot plants was performed for 1996 and 1997. Results of the study were summarized as follows. For the BOD and COD, when the pollutant loading of them was about 1 3.8g/$m^2$. day (the concentration was l24.0mg/${\ell}$) arid 24.4g/$m^2$.day(the concentration was 220.Omg/${\ell}$), the removal rate of them was high, 90.2% and 93.4%, respectively. For the SS, the effluent concentration was consistently lower than the water quality standard even though the influent concentration varied significantly, which showed that SS was removed by the system effectively which consist of soil and plants. For the T-N and T-P, when the influent pollutant loading of them were moderately high, 2.8g/$m^2$.day to 7.4g/$m^2$. day(concentration 25.0mg/${\ell}$ to 49.7mg/${\ell}$) for T-N and 1.0g/$m^2$.day to 2.6g /$m^2$.day(concentration 8.6mg/${\ell}$ to 14.7mg/${\ell}$) for T-P, the removal rate of them were 86.5% and 94.0%, respectively. The removal rate by the flow distance increased rapidly in the first 4m from the inlet zone, and gradually there after. The width of system was 2m. Overall, the result showed that constructed wetland system is a feasible alternative for the treatment of wastewater from industrial complex in rural areas. Compared to existing systems, this system is quite competitive because it requires low capital cost, almost no energy and maintenance, and therefore, very cost effective.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.313-313
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• 2021

남·북한강 상류 수계에는 만대지구, 가아지구, 자운지구, 도암호유역, 골지천유역, 대기지구 등 총 6개의 비점오염원관리지역이 지정되어 있으며, 비점오염원관리를 위한 관리대책 및 시행계획에 따라 지속적으로 비점오염저감시설이 설치·운영되고 있다. 그러나, 기후변화에 따른 집중호우 등의 원인으로 뚜렷한 비점오염 저감효과가 나타나지 않고 있으며, 개별 비점오염 저감시설의 명확한 저감효율이 정량화되지 않아 탁수저감 기여도 및 효율성 평가 시 한계가 있다. 따라서 본 연구에서는 문헌연구를 통하여 비점오염저감시설별 저감효율 제시가 미흡하였던 시설 중 돌망태, 식생토낭에 대해 실험을 진행하였다. 저감효율 평가를 위해 경사도 3%, 사면 폭 4 m, 사면길이 22 m의 시험포를 조성하였다. 강우시 발생하는 유출수를 대조구와 비교하였으며, 영농시기를 고려하여 영농작업을 병행하여 실시하였다. 제거효율법을 통한 저감효율 산정 결과 돌망태와 식생토낭의 SS항목은 80.5%와 73.7%로 저감효율이 가장 높은 것으로 나타났으며, BOD는 71.2%, 60.2%, T-N은 70.5%, 64.7%, T-P는 63.7%, 63.1%로 나타났다. TOC는 46.8%, 38.8%로 다른 항목에 비해 상대적으로 낮은 저감효율이 나타났다. 부하량합산법을 통한 저감효율 산정 결과 돌망태와 식생토낭의 T-N 항목은 78.6%와 70.8%로 저감효율이 가장 높은 것으로 나타났으며, BOD는 77.5%, 65.4%, SS는 76.7%, 67.8%, T-P는 68.4%, 63.7%로 나타났다. TOC는 62.7%, 44.6%로 시설별 뚜렷한 차이가 나타났다. 제거효율법과 부하량합산법을 이용하여 저감효율을 분석한 결과 모든 항목에서 40%이상의 저감효율이 나타났으며, 돌망태가 식생토낭에 비해 저감효과가 좋은 것으로 분석되었다. 경사면의 흙탕물 저감을 위해서는 비점오염저감시설의 설치가 우선되어지나, 탁수저감 기여도 및 저감효율 제시를 위한 정량화는 장기간의 모니터링이 필요하기 때문에 지속적인 모니터링을 통한 효율평가가 이루어져야 할 것으로 판단되며, 본 연구 결과는 향후 비점오염저감시설 설치에 따른 유역 내 오염물질 발생 모의를 위한 모델링의 기초차료로 활용될 수 있을 것으로 기대된다.

• Journal of Animal Environmental Science
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• v.12 no.3
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• pp.123-132
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• 2006

This study was conducted to determine the volume of Holstein heifers manure excreted and its characteristics. The average dry matter intake of heifers was 6.7 kg/head/day. The intake rate was lowest in spring among four seasons. The average dry matter intake rate during spring, summer, fall, and winter was 4.6, 8.3, 7.1, and 6.8 kg/head/day, respectively. The average water intake of heifers was $19.3{\ell}/head/day$. The wale. consumption was highest value ($21.8{\ell}/head/day$) in summer and lowest values ($18.3{\ell}/head/day$) in spring and winter. Values were found not to be statiscally different for the four seasons. The average manure production of heifers (average live weight was 363.1 kg) was 20.3 kg/head/day and it was 5.6% of live animal weight. The manure production during spring, summer, fall, and winter was 13.7, 23.5, 25.0, and 20.2 kg/head/day, respectively. Production during spring was lower than the other seasons (p<0.05). A higher correlation between live weight and manure production ($R^2=0.7816$) and between live weight and feed intake ($R^2=0.7296$) was observed for heifers. Correlations between manure production and water intake and between manure production and feed intake were found to be relatively low for heifers. The moisture content of feces was 83.5% and that of urine 94.6%. The pH of feces and urine were in the ranges of 7.4 and 7.5, respectively. The $BOD_5$, COD, SS, T-N, T-P concentrations of the heifer feces were 18,048, 50,114, 119,833, 2,519, and $427mg/{\ell}$, respectively. Heifer urine showed lower levels of $BOD_5(5,434mg/{\ell})$, COD$(6,550mg/{\ell})$, SS$(825mg/{\ell})$, T-N$(3,616mg/{\ell})$, and $T-P(28mg/{\ell})$ than feces. The fertilizer nutrient concentrations of heifer feces was 0.25% N, 0.1% $P_2O_5$ and 0.14% $K_2O$. Urine was found to contain 0.36% N, 0.006% of $P_2O_5$ and 0.31% $K_2O$.