• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.3
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• pp.302-310
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• 2017

Interferon regulatory factor 8 (IRF8) is essential for the development of B and T cells, as well as for the activity of dendritic cells and macrophages. We performed molecular characterization of IRF8 from rock fish, Sebastes schlegelii (Ss), and investigated the spatial and temporal profile of mRNA expression after challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), or Streptococcus iniae. The full-length cDNA sequence of SsIRF8 was 1,657 bp, containing an ORF of 1,266 bp. The gene had a predicted molecular mass of 47.7 kDa and an isoelectric point of 5.99. The amino acid sequence coded by this gene showed the highest degree of identity (90.8%) and similarity (96.2%) with IRF8 from Oplegnathus fasciatus. The SsIRF8 mRNA was expressed ubiquitously, at varying levels, with the highest level of expression observed in the spleen. To confirm the role of SsIRF8 in mediating the immune response, we measured SsIRF8 mRNA expression in the splenic tissue at different time points after injection with LPS, poly I:C, or S. iniae. The qRT-PCR results showed that SsIRF8 mRNA expression in the poly I:C-injected group was highly upregulated 6 hr after exposure (P<0.05). Expression of SsIRF8 mRNA in the S. iniae-injected group peaked at 24 hr. These results suggest that SsIRF8 might be important in regulating the strength of the rockfish immune response to immunostimulatory agents.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.3
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• pp.473-480
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• 1988

In this study, a method that can realize the effect of residual stresses on fatigue crack propagation has been proposed. This method requires that the relaxation quantities of residual stresses are measured by experimental method according to fatigue cycles and are employed to the experimental results of base materials by introducing the concept of superposition. SS41 material has been analyzed as an example. The results show that the relaxation factor of residual stresses for fatigue cycles is .alpha.$_{i}$= 1/0.6903+0.0380 $a_{I}$ the effective stress ratio for fatigue crack propagation length is ( $R_{eff}$)$_{i}$ = 1/1.1274+0.0118$_{I}$ and the fatigue crack propagation rate for relaxation quantities of residual stresses is (da/dN)$_{t}$ = 0.5 $K_{cf}$ - .DELTA.K/{1-1/1.1274+0.0118$_{ai}$ } $K_{cf}$ - .DELTA. K(da/dN), for stress ratio /R=0.5.io /R=0.5.5.

• Journal of the Korean Society of Radiology
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• v.10 no.4
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• pp.279-284
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• 2016

Single-shot echo planar imaging(SS-EPI) is well established as high sensitivity for ischemic stroke. However, it is prone to susceptibility artifact in brain stem that diminish the image quality. single-shot turbo spin echo(SS-TSE) is a new DWI technique that can reduce susceptibility artifact. Thus, this research was conducted so as to reduce geometric distortion in brain stem by using single-shot turbo spin echo technique. Thirty patients without brain disease underwent diffusion MR on a 3T scanner with SS-EPI and SS-TSE. Obtained images with both sequences were analyzed for geometric distortion and error percentage as well. Image quality in terms of geometric distortion of SS-TSE were found to be significantly better than those for SS-EPI. And error percentage was considerably reduced for 2.4% of b0 image(from 11.1% to 8.7%), 1.2% of b1000 image(from 11.4% to 10.1%), respectively. In summary, diffusion MR using SS-TSE significantly reduce geometric distortion compared to SS-EPI in brain stem and may provide improved diagnostic performance.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.171-178
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• 2017

A series of novel effector molecules secreted by the type three secretion system (T3SS) of Shigella spp. have been reported in recent years. In this study, a proteomic approach was applied to study T3SS effectors systematically. First, proteins secreted by the S. flexneri wild-type strain after Congo Red induction were separated and identified using two-dimensional electrophoresis to display the relative abundance of all kinds of early effectors for the first time. Then, a gene deletion mutant of known virulence repressor (OspD1) and a gene overexpressed mutant of two known virulence activators (MxiE and IpgC) were constructed and analyzed to discover potential late effectors. Furthermore, the supernatant proteins of gene deletion mutants of two known translocators (IpaB and IpaD), which would constantly secrete effectors, were also analyzed. Among all of the secreted proteins identified in our study, IpaH1.4, IpaH_5, and IpaH_7 have not been reported before. These proteomics data of the secreted effectors will be valuable to understand the pathogenesis of S. flexneri.

• Journal of the Korean Geographical Society
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• v.41 no.4 s.115
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• pp.418-434
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• 2006

The study was investigated to runoff characteristics of non-point pollutants according to rainfall in Kokseong river watershed. The result of which is as follows : First of all, major reason which affect the formation of water quality of Kokseong River is judged to be caused by non-point pollution source which flows out from farmland and residential area. Flow of rainfall effluent in the downstream in which direct flow components of urban district and combined sewer overflows of farmland was intervened faster than that in the upstream reacted more promptly. Generation of pollutants by non-point source shows increasing trend in general in accordance with the increase in the intensity of rainfall but it was affected by SS, BOD, COD and T-P in the upstream part whereas BOD, COD and T-N were significantly affected by beginning period of rainfall in the downstream. EMC in the downstream increased approximately 3-315 times as compared to upstream, particularly the discharge of SS5 and T-P were extremely increased. While surface flow out of rainfall effluent in the upstream was only 4.7%, the surface flow in the downstream took up as much as 29%, which was major reason for the increase of EMC. From the above contents, we can see that the change in water quality according to the increase and decrease of effluent at the time of rainfall showed very complex pattern depending on the type of land use, and it is judged that the most important thing for the administration of non-point pollution source is to come up with the solution for the reduction of effluent at the beginning.

• The Plant Pathology Journal
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• v.39 no.6
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• pp.584-591
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• 2023

Active plant immune response involving programmed cell death called the hypersensitive response (HR) is elicited by microbial effectors delivered through the type III secretion system (T3SS). The marine bacterium Hahella chejuensis contains two T3SSs that are similar to those of animal pathogens, but it was able to elicit HR-like cell death in the land plant Nicotiana benthamiana. The cell death was comparable with the transcriptional patterns of H. chejuensis T3SS-1 genes, was mediated by SGT1, a general regulator of plant resistance, and was suppressed by AvrPto1, a type III-secreted effector of a plant pathogen that inhibits HR. Thus, type III-secreted effectors of a marine bacterium are capable of inducing the nonhost HR in a land plant it has never encountered before. This suggests that plants may have evolved to cope with a potential threat posed by alien pathogen effectors. Our work documents an exceptional case of nonhost HR and provides an expanded perspective for studying plant nonhost resistance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.494-503
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• 2017

This study was conducted to investigate the relationship between the Health Locus of Control (HLC), social support (SS), self-efficacy (SE) and Health Promoting Behavior (HPB) in middle-aged adults and the factors influencing their HPB. Data were collected from 137 middle-aged adults in the cities of Chungbuk and Chungnam for 2 months from July to August 2016 using structured questionnaires. The data collected were analyzed by descriptive statistics, the t-test, ANOVA, Pearson correlations, and multiple regression analysis. The results showed that the HPB varied significantly according to the subjective health (F=10.14, p<.001) and economic level (F=3.32, p= .039) among the general characteristics. In the relationship between the major variables, the HPB showed a significant positive correlation with the SE (r=.44, p<.001) and SS (r=.45, p<.001). A multiple regression analysis was conducted to examine the effects of the general characteristics, HLC, SE, and SS on the HPB, and the results showed that the factors influencing the HPB were the subjective health status (${\beta}=.24$, p=.002), SE (${\beta}=.29$, p<.001), and SS (${\beta}=.30$, p<.001), and these factors showed a combined explanatory power of 36.4%. The results of this study suggest that the SE and SS are important factors affecting the HPB. Therefore, it is necessary to develop and apply a health promotion program reflecting the SE and SS, in order to (promote) the (HPB) of middle-aged adults.

• Restorative Dentistry and Endodontics
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• v.25 no.3
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• pp.381-389
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• 2000

Lateral condensation with gutta-percha and sealer has been shown to provide an excellent apical seal; however, the lateral condensation technique has demonstrated less favorable apical leakage results in curved canals when compared with straight canals. Placement of endodontic spreaders to within 1 to 2mm of the root canal working length has been advocated for optimum gutta-percha obturation. Due to their stiffness, stainless-steel(SS) spreaders will often fail to achieve this position in curved canals. Newly marketed nickel-titanium(NT) spreaders may offer an advantage in this regard due to the increased flexibility of these instruments. The purpose of this study was to evaluate the effect of NT finger spreader on the sealing ability in lateral condensation technique, compared with conventional SS finger spreader. Twenty four standardized resin models simulating curved canals(30 degree) were randomly placed into 2 groups and instrumented to a #30 master apical file size with Ni-Ti Profile .04 taper series using step down technique. Each groups was obturated with standardized gutta-percha cone by standard lateral condensation technique using SS finger spreader, NT finger spreader. And then, each model was sectioned horizontally with microtome at 1, 2, 3, 4, 5mm levels from the apex. At each of 5 levels, ratio of the area of gutta-percha was obtained by calculating the area of gutta-percha to the total area of the canal. The data collected were then analyzed statistically using a t test for independent samples. The results as follows ; 1. The total mean ratio of area of gutta-percha was 89.20${\pm}$7.00(%) for SS spreader group. 92.20${\pm}$5.17(%) for NT spreader group. There was statistically significant difference between each group(p<0.05). 2. At 3mm level, the mean ratio of area of gutta-percha was 88.32${\pm}$5.41(%) for SS spreader group, 95.25${\pm}$2.60(%) for NT spreader group. There was statistically significant difference between each group(p<0.05). At 1,2,4mm levels, NT spreader group showed greater mean ratio of area of gutta-percha than SS spreader group, too. But there was no statistically significant difference. 3. At 5mm level, the mean ratio of area of gutta-percha was 91.83${\pm}$3.42(%) for SS spreader group, 87.91${\pm}$3.68(%) for NT spreader group. There was statistically significant difference between each group(p<0.05). This study concluded that the NT spreader demonstrated somewhat favorable apical sealing effect than SS spreader in prepared curved canals. The clinical use of NT spreaders may enhance our ability to create better apical seals in curved canals, but further studies in this area will help clarify some of the remaining areas with which practitioners are concerned, such as compaction forces exerted by NT spreaders.

• Journal of Korea Water Resources Association
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• v.53 no.8
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• pp.605-616
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• 2020

The purpose of this study is to assess the future hydrological and water quality change of Man-gyeong river basin (1,602 ㎢) based on future extreme climate change scenarios and reduction of inter-basin water transfer amount using SWAT (Soil and Water Assessment Tool). The SWAT was calibrated (2012~2014) and validated (2016~2018) at 2 water level observation stations (DC, JJ) and 2 water quality observation stations (SR, GJ) considering inter-basin water transfer amount, stream water withdrawal, and point source data. For the streamflow, the coefficient of determination (R²) was 0.70 and the average Nash-Sutcliffe efficiency (NSE) was 0.51 respectively. For the water quality of SS, T-N, and T-P, the R² was 0.72, 0.80 and 0.72 respectively. The future average streamflow under climate change scenarios increased up to 459 mm/yr, and average SS, T-N and T-P yields also increased up to 19,548 ton/yr, 68,748 kg/yr, and 13,728 kg/yr respectively. When the amount of inter basin water transfer decreased, the streamflow especially decreased in spring and winter periods, and the future water quality yields increased under the influence of precipitation. In order to solve the deterioration of water quality due to decrease in the flow rate and an increase in the load, the amount of inter basin water transfer should be maintained to a certain level.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.8
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• pp.549-553
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• 2014

Variation of sewage sludge interface height and flow rate by rainfall were applied to the actual public sewage treatment plant, and the efficiency of sewage treatment was evaluated by measuring $BOD_5$, $COD_{Mn}$, SS, T-N, and T-P. When both flow and interfacial height are increased, the treatment efficiencies in terms of the five water pollutants are decreased. Among them SS is the most critical pollutant in rainfall. When 0.5 Q inflow was applied, the efficiencies were 74.2% at the sludge interface height of 0.5 m, 76.4% at 1.0 m, 70.2% at 1.5 m, and 60.7% at 2.0 m. When 1.0 Q inflow was applied, the efficiencies were 71.7% at the sludge interface height of 0.5 m, 71.9% at 1.0 m, 46.4% at 1.5 m, and -38.0% at 2.0 m. Operation at 2.0 Q~2.0 m and 3.0 Q~1.0 m above the sludge rising phenomenon occurred causing adverse effects on the public bodies. If the flow rate increases, the processing efficiency is reduced from 74.2% to 17.3%, even though the sludge interface height was maintained at 0.5 m, so that the inflow adjustment was most important during rainfall, and the interface height of 1.0 m should be maintained to minimize the adverse effect on public water system.