• Journal of Veterinary Clinics
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• v.38 no.4
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• pp.179-183
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• 2021

A 12-year-old male American Cocker Spaniel was diagnosed with a type of chronic hepatits (CH) called cholangioheaptits. Routine supportive medication was administered to the patient, and ex vivo boosted immune cell (EBI-C) therapy was used for the treatment. A histopathologic examination of the liver 19 months later revealed that the cholangiohepatitis had progressed to cholangiocarcinoma. The medication and immune cell therapy was maintained. Two months after the new diagnosis, the patient's state worsened, and the dog died 635 days after the first visit. EBI-C therapy is a type of immunotherapy, where immune cells are isolated from the patient's peripheral blood mononuclear cells, expanded ex vivo, and then infused into the patient intravenously every two weeks. EBI-Cs (mean: 2.78 × 10⁸ cells) were obtained 38 times and infused every two weeks. Most EBI-C were T-lymphocytes (99.24% of total EBI cells). T-lymphocytes produce large interferon (IFN)-γ, and IFN-γ inhibits liver fibrosis in dogs with CH. Moreover, in bile duct cancer, an increase in T-lymphocytes correlates with decreasing tumor invasion and metastasis. Thus, we propose that EBI-C therapy is applicable as a new supportive therapy for canine liver disease if other treatments like drug medication, surgery, or radiation are unavailable.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.43-52
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• 2004

This study was carried out to determine the effect of corn silage and roughage feeding systems on milk yield and milk compositions. The experimental design was allotted into 3 treatments according to the feeding systems of rice straw(rice straw＋concentrate: T1), corn silage(corn silage ＋ concentrate: T2) and TMR treatment(Total mixed rate : roughage ＋ concentrate: T3). This research was carried out from Oct. 1988 to Mar. 1999 at Kimcheon Kyungbook. The results obtained were summarized as follows: 1. The milk yield increased upon T2(31.9$\pm$3.2kg) ＞ T3(29.6$\pm$3.8kg) ＞ T1 treatment(22.5$\pm$2.0kg)(P<0.05), but cows with T2 and T3 produced the highest at third parity while T1 at forth parity. 2. The milk fat percentage was the highest at T3 treatment(3.79$\pm$0.31%), and on the other hand T1 treatment appeared the lowest percentage. Cow at 2nd parity produced the highest milk fat contend over the other parity. 3. There was no significant difference in protein content between treatments and parity, but T2 and T3 were higher than T1 4. Treatment did not affact content of SNF even though T2 and T3 of the SNF revealed to higher than T1. T5 was T3(12.51$\pm$0.57%)>T1(11.71$\pm$0.62%)>T2 treatment(11.52$\pm$0.55%). These were not significant. 5. Somatic cell counts were the highest at T1(39.6 ${\times}$ $10^4$cell/ml), but T2 treatment was the lowest as 28.7 ${\times}$ $10^4$ cell/ml. These results indicates that com silage(T2) and TMR treatment(T3) could be recommended

• Journal of the Korean Society of Hazard Mitigation
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• v.2 no.1 s.4
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• pp.143-152
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• 2002

The objectives of this paper were to obtain the fundamental data to analyze the causes of deterioration of 39 freeway concrete viaducts in Seoul metropolitan area. To investigate the degree of concrete deterioration, carbonation depth, soluble chloride concentration in hardened concrete and half-cell potentials of reinforcement were measured. The number of structures which carbonation depth penetrates to reinforcement was 25% of total. The model of carbonation .ate was induced to 3.92 $\sqrt{t}$, which was 5% faster than 3.727 $\sqrt{t}$ assumed 60% water-cement ratio, R=1 in that of kishitani. After measuring chloride concentration in concrete, it was concluded that about 24% of all readings on samples from concrete exceed the critical content to minimize the risk of chloride-induced corrosion. About 31% of the freeway viaducts structures had a value lower than -350mV(vs. CSE), so it could conclude that the excessive chloride concentration was the major cause of reinforcement corrosion. Among the structures which measured half-cell potentials less than -350mV, about 50% exceeds the maximum acceptable limit of chloride concentration.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.177-182
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• 1996

Effects of 14 carbohydrates supplied as carbon sources on cell growth and sporulation of, and the production of insecticidal crystal proteins by Bacillus thuringiensis strains were investigated in liquid cultures. Strains grew well in media containing any one of the 14 carbohydrates supplied, reaching maximum cell densities of $10^7{\sim}10^8\;cells/ml$ in 16.7 to 22 hours after inoculation depending on the strain. Spores first appeared in 16.7 to 24.7 hours after inoculation, and 80% sporulation was reached in 28 to 51.3 hours after inoculation depending on the strain. No change in pH of media was observed after cell multiplication. The production of total protein was highest when supplied with sucrose and was lowest with starch. More insecticidal crystal proteins were produced when supplied with glucose, lactose, maltose, or sucrose. The amount of insecticidal crystal proteins produced by the strains was proportional to that of the total protein. The relative amount of individual insecticidal crystal protein species produced by B.t. kurstaki and B.t. israelensis was not influenced by the carbohydrates supplied.

• The Journal of Internal Korean Medicine
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• v.30 no.3
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• pp.451-464
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• 2009

Background and Objective : Cyperus rotundus L. (CR) is a commonly used herbal medicine in Asian countries such as Korea, China and Japan. The present study was designated to evaluate the direct effects of CR on helper T cell activities and on Th1/Th2 lineage development in vitro. Materials and Methods : Spleen cells from 8 week BALB/c mice were cultured in CR extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and analyzed for mRNA expression levels of INF-$\gamma$, IL-4, T-bet and GATA-3 by RT-PCR and secretion cytokines levels of INF-$\gamma$, IL-4, IL-5 and IL-10 by ELISA. Results : The results demonstrated that CR had no mitogenic effects on unstimulated CD4+ T cells, but augmented CD4+ T-cell proliferation upon activation with anti-CD3/anti-CD28 antibodies in a dose-dependent manner. CR treatment significantly increased CD4+ T cell population and the IFN-$\gamma$ expression was significantly enhanced, while IL-4 expression was significantly decreased. In addition, in vitro Th1/Th2 polarization experiments revealed that CR enhanced IFN-$\gamma$ secretion in Th1 cells, but reduced the IL-4 in Th2 cells in a dose-dependent manner. Conclusion : These results suggest that CR treatment could be a desirable alternative therapy for the prevention or correction of Th2 dominant pathological disorders, such as allergy and asthma.

• The Journal of Internal Korean Medicine
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• v.28 no.4
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• pp.681-693
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• 2007

Background and Objective : Atractylodes japonica (AJ) is a commonly-used herbal medicine in Asian countries such as Korea, China and Japan. The present study was designated to evaluate the direct effects of AJ on helper T cell activities and on Th1/Th2 lineage development in vitro. Materials and Methods : Spleen cells from 8-week BALB/c mice were cultured in CR extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and analyzed for mRNA expression levels of INF-$\gamma$, IL-4, T-bet and GATA-3 by RT-PCR and secretion cytokines levels of INF-$\gamma$, IL-2, IL-4, IL-5 and IL-10 by ELISA. Results : The results demonstrated that AJ had no mitogenic effects on unstimulated CD4+ T cells, but augmented CD4+T-cell proliferation upon activation with anti-CD3/anti-CD28 antibodies in a dose-dependent manner. AJ treatment significantly increased CD4+ T cell population and IFN-$\gamma$ expression was significantly enhanced, while IL-4 expression significantly decreased. In addition, in vitro Th1/Th2 polarization experiments revealed that AJ enhanced IFN-$\gamma$ secretion in Th1 cells, but reduced the IL-4 in Th2 cells in dose-dependent manner. Conclusion : These results suggest that AJ treatment could be a desirable alternative therapy for the prevention or correction of Th2 dominant pathological disorders, such as allergy and asthma.

• The Journal of Internal Korean Medicine
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• v.28 no.1
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• pp.12-24
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• 2007

Objective : To investigate the effect of Samilshinkihwan(SISKW) on white rats with deteriorated immunity caused by methotrexate. Methods : The test articles were dosed once a day for 14 days by gastric gavage at a dosage 1000, 500 and 250mg/kg/10ml of SISKW from 2 days after last MTX-dosing, and changes in body weight, spleen weight, total blood leukocyte numbers, total lymphocyte numbers, B and T lymphocyte ratio, CD3+CD4+, CD3+/CD8+ and CD4+/CD8+ T lymphocyte ratio in the blood and spleen were measured. In addition, the serum interleukin (IL)-2 levels and the productivity of IL-2 of splenic cells were also demonstrated in this study. Results : The changes on body weight increased significantly in the 1000mg/kgof SISKW group. The changes on the spleen weight, the total blood leukocytenumbers, the total lymphocyte numbers in the blood and spleen, the ratio of T-cell in the blood and spleen and the ratio of CD3+CD4+ T-cell in spleen increased significantly in all SISKW groups as compared with the control group. The ratio of CD4+/CD8+ T-cells in blood increased significantly. The serum IL-2 levels and productivity of IL-2 of splenic cells increased significantly in 1000 and 500mg/kg SISKW groups as compared with the control group. Conclusions : Samilshinkihwanhas an effect of increasing immune responses, especially on cellular immune responses, in white rats with deteriorated immunity caused by methotrexate.

• Journal of Periodontal and Implant Science
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• v.44 no.6
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• pp.274-279
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• 2014

Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerella forsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates for the study; thus 80 chronic periodontitis patients and 28 healthy persons (control group) were invited to participate in this investigation. The presence of T. forsythia was detected by polymerase chain reaction (PCR) analysis using primers designed to target the respective 16S rRNA gene sequences. Peripheral blood was collected from each subject to identify the IgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunological laboratory processes were completed blindly, without awareness of the clinical status of the study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level (CAL) and probing depth were found in the presence of the IgG1 antibody titers against whole-cell T. forsythia; however, only the difference in CAL was statistically significant. In the presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parameters evaluated were higher but they did not show statistical differences, except for plaque. The unadjusted linear regression model showed that the IgG1 antibody against whole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL (${\beta}=-0.654$; 95% confidence interval [CI], -1.27 to -0.28; P<0.05). This statistically significant association remained after adjusting for possible confounders (${\beta}=-0.655$; 95% CI, -1.28 to -0.29; P<0.05). On the other hand, smoking was a statistically significant risk factor in the model (${\beta}=0.704$; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of the IgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the results of this study suggest that IgG1 antibody to T. forsythia may have been a protective factor from periodontitis in this sample.

• IMMUNE NETWORK
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• v.24 no.5
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• pp.35.1-35.17
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• 2024

Plasma cells (PCs) constitute a small proportion of B cells, limiting their biochemical characterization. An in vitro culture system that reliably generates PCs could provide an alternative method to obtain PCs for further analysis and manipulation. To date, most in vitro PC differentiation methods rely on B cell receptor (BCR)-independent stimulants, including TLR ligands, CD40L, and cytokines. However, these methods do not fully recapitulate the natural T cell-dependent PC differentiation process, in which BCR activation is the initial events. In this study, we established an efficient in vitro PC differentiation method incorporating BCR stimulation. Naïve B cells were first stimulated with anti-IgM and antiCD40 Abs, followed by stimulation with various cytokines. By screening cytokines known to participate in PC differentiation in vivo, we identified that the combination of IL-4 and IL-5 induced the most efficient PC differentiation. The in vitro generated PCs highly expressed PC-associated surface markers and regulatory genes. Additionally, they secreted high amounts of IgM and IgG Abs. Moreover, retroviral transduction of B cells resulted in efficient target gene expression in PCs. Our new method closely mimics natural PC differentiation and effectively generates a large quantity of PCs for various applications, including elucidating the molecular mechanisms underlying PC differentiation.

• Fisheries and Aquatic Sciences
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• v.7 no.1
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• pp.34-38
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• 2004

The heterotrophic production method for Tetraselmis suecica, a suggested alternative to photoautotrophic one in the economic sense, was studied in terms of cell growth and sterolic property. The alga in the 10 mM organic carbon (glucose) manifested cell growth. However, the alga produced by the heterotrophic method showed a unique property of sterol determined with an aid of GC and GC-MS. The photoautotrophic control T. suecica contained 6 detectable sterol species: $cholesta-5,\;22-dien-3\beta-o1$, $ergost-5-en-3\beta-o1$, cholest-5-en-3\beta-o1$, $24-methyl-cholesta-5,\;22-dien-3\beta-o1$, $24-methylcholesta-5,\;24-dien-3\beta-o1$, $24-ethylchlolesta-5,\;24-dien-3\beta­o1$, $24-methylcholesta-5-en-3\beta-o1$, and $24-ethylchlolesta-5en-3\beta-o1$. We discuss the sterolic properties of the alga along the heterotrophic progress, particularly focusing on the availability of the method in the aquaculture of bivalves which normally need sterols as a dietary source.