• Laboraroty Animal Research
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• v.34 no.4
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• pp.185-194
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• 2018

The different polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene promote variances in diabetes susceptibility in humans. We investigated whether these genotypes also promote differences in diabetic susceptibility in commercial pigs. Growing pigs (Landrace, both sex, 50-60 kg) with the C/C (n=4) and T/T (n=5) TCF7L2 genotypes were identified and intravenously injected with streptozotocin (STZ, 40 mg/kg) twice in weekly intervals, then a high-energy diet was offered. Oral glucose tolerance tests, blood analyses and the homeostasis model assessment-insulin resistance (HOMA-IR) index calculations were performed. The animals were sacrificed at the end of 12 weeks of treatment to reveal the pancreas histomorphometry. The results showed that all of the treated pigs grew normally despite exhibiting hyperglycemia at two weeks after the induction. The glycemic level of the fasting or postprandial pigs gradually returned to normal. The fasting insulin concentration was significantly decreased for the T/T carriers but not for the C/C carriers, and the resulting HOMA-IR index was significantly increased for the C/C genotype, indicating that the models of insulin dependence and resistance were respectively developed by T/T and C/C carriers. The histopathological results illustrated a significant reduction in the pancreas mass and insulin active sites, which suggested increased damage. The results obtained here could not be compared with previous studies because the TCF7L2 background has not been reported. Growing pigs may be an excellent model for diabetic in children if the animals are genetically pre-selected.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.15 no.2
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• pp.1-8
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• 2019

The integrity of the Reactor Pressure Vessel (RPV) is affected by the neutrons bombarding the vessel wall leading to embrittlement. This irradiation-induced embrittlement leads to reduction in the fracture toughness of RPV materials. This paper presents a comparative study of typical Optimized Power Reactor (OPR)1000 reactor pressure-temperature (P-T) limit curves using the pre-2006 American Society of Mechanical Engineers (ASME) editions used in the power plant and the current ASME edition of 2010. The current ASME Code utilizes critical reference stress intensity factor based on the lower bound of static, while the Pre-2006 ASME editions are based the critical reference stress intensity factor based on the lower bound of static, dynamic and crack arrest. Model-Based Systems Engineering approach was used to evaluate ASME Code Section XI Appendix G for generating the P-T limit curves. The results obtained from this analysis indicate decrease in conservatism in P-T limit curves constructed using the current 2017 ASME code, which can potentially increase operational flexibility and plant safety. Hence it is recommended to use ASME code edition after 2006 be used in all operating nuclear power plants (NPPs) to establish P-T limit curve.

• IMMUNE NETWORK
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• v.21 no.3
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• pp.24.1-24.13
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• 2021

Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.986-993
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• 2001

Translation Initiation in prokaryotes involves the formation of a 30 S preinitiation complex, in which translation initiation factors play a role in the stimulation of fMet-tRNA (fMet) binding. However, the specific function and precise mechanism of initiation factor IF1 are still unclear. One a functionally active factor with a high purity. In the present study a large quantity of active IF was rapidly purified, obtained by the overexpression of the infA gene, and then used for a functional study. The induction of infA did not appreciably affect the growth rate of the protease-deficient strain E. coli AR68 harboring the IF1 overproducing plasmid. The level of IF1 obtained was approximately $1-2\%$ of the total cell protein, which enabled the yield of highly purified IF1 (>$98\%$ pure) to be increased to 0.15 mg of IF1/g of cells. The IF1 was isolated within one day by the centrifugatioin of the ribosomal washed fraction, by ammonium sulfate fractionation, chromatography on batch of phosphocellulose, and FPLC Mono S. The overexpressed IF1 was found to be comparable to the factor isolated from normal cells, as determined by migration in NEPHGE/SDS 2-D gels. For binding of fMet-tRNA(fMet) to the 30 S ribosomal subunitis, relatively high levels of binding were obtained when IF2 was present. The addition of IF1 up to 110 pmol proportionally stimulated the binding to a variable extent. This IF1-dependent stimulation of the 30 S preinitiation complex formation demonstrated that IF1 would appear to be exclusively essential for promoting the initiation phase of protein synthesis.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.3
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• pp.471-482
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• 1995

Using the acetate velvet and viscose velvet whose pile lengths were sheared as 1.45, 1.55, 1 65, 1.75, 1.85, 1.90mm under the condition equating the weaving process of ground fabric, the conclusions were as follows through the results of the sensory assessments estimated by women students in our university and the physical properties, H. V and T. H. V obtained by KES-F system. 1. In the sensory assessments estimated by the method of paired comparison and ranking of samples, the longer pile length was, the more the hand values of smoothness, softness, thickness, heaviness increased on the whole. 2. The H V. and T. H. V. measured by KES-F system were as follows; Kohi increased to pile length 1.85mm and then decreased a little at 1.90mm. hummer increased as pile length was longer. Fukurami increased to pile length 1.75mm and then decreased gradually as pile length was longer. Total hand value increased gradually from 1. 45mm to 1.85mm, had the top value at 1.85mm, and then decreased a little at 1.90mm. 3. In the results of summarizing $\ulcorner$the physical properties correlated closely with the H. V obtained by sensory assessments$\lrcorner$ and tithe Physical properties correlated closely with the H. V. and T. H. V obtained by KES-F systems, it showed that all the sensory properties correlated closely with compressive energy, flexural rigidity, thickness, weight and pile ratio in the former and that the physical properties correlated closely with each H V and T. H. V were different in the latter. 4. It showed that factor 1 was related to compressive energy, thickness, weight, pile ratio, factor 2 was related to recovery energy, compressive resilience, compressive index, and factor 3 was related to compressive recovery ratio in the result of factor analysis. 5. In the multiple repression analysis, the expressions of all sensory properties had compressive ratio, frictional coefficient in the regression expressions of $\ulcorner$H. V. obtained by sensory assessments$\lrcorner$, while the expressions of each H. V. and different physical properties in the regression expressions of $\ulcorner$H. V. obtained by KES-F system$\lrcorner$.

• Journal of Home Health Care Nursing
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• v.12 no.2
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• pp.1-15
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• 2005

Purpose: This study was conducted to assess the fall risk factors. Method: The subjects were 87 persons who were older than 65 years living at 28 nursing home in Seoul and Gyunggi province. Subjects were interviewed using RAFS II for intrinsic factor and the environmental factor were assessed using a structured questionnaire from Oct. to Dec. in 2004. The data were analyzed by SPSS(ver. 12.0) programs, using descriptive statistics, $x^2$-test, and t-test. Result: The 37.9% of the subjects experienced the fall, and its average number is $1.94\pm1.75$. The Women's experience of the fall was higher than that of the men, but it was no significant difference with gender. There was no significant difference with the age and duration of living. The mean of the intrinsic risk factor was 13.38 in total score 39 points marks on the RAFS II scales. The intrinsic risk factor score of the fallen group 15.71 was significantly higher than the non-fallen group 12.10. The variables of recent fall experience(t=4.72, p=0.000) and urinary dysfunction(t=2.64, p=0.010) was significantly higher than the non-fallen group. The highest variable of the intrinsic factor was the age and the variables of drug intake, balance, chronic disease, recent fall experience, urinary dysfunction were followed in order. The mean of the environmental risk factor was 0.24 points. No significant differences were shown in environmental risk factor between the fallen and the non-fallen groups. To the fallen group, the place of entrance was the highest risky environmental factor. To see in area dimension, the floor surfaces was the highest risky environmental factor and equipment and illumination factor was the following risky elements in order. In the total score of environmental risk factors based on the ares, the fallen group was 0.26 and the non-fallen group was 0.24, but there were no significant differences between the groups. Conclusion: This results suggested that visual protection strategy, set up the safety device in the place of entrance and inner stairway, bathroom and nonskid mat in the nursing home would be contribute to the prevention of the fall for the elderly.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.6
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• pp.106-112
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• 2016

In this study, circular concrete column specimens confined by carbon sheet tube with different winding angles and different number of carbon sheet plies(3T, 5T and 7T) were tested to propose design equations and a strength reduction factor. Specimens were designed by 300 mm diameter and 600 mm height with $90^{\circ}{\pm}0^{\circ}$, $90^{\circ}{\pm}30^{\circ}$, $90^{\circ}{\pm}45^{\circ}$, $90^{\circ}{\pm}60^{\circ}$, $90^{\circ}{\pm}75^{\circ}$ and $90^{\circ}{\pm}90^{\circ}$ carbon fiber angles. A 10,000 kN UTM was used for compressive strength test of specimens by displacement control method with 0.01 mm/sec velocity. Estimation equations of compressive strength and ultimate strain of circular concrete column specimens confined by carbon sheet tube using a regression analysis and a strength reduction factor to apply ultimate strength design method of concrete were proposed. The strength reduction factor(${\phi}$) of circular concrete columns confined by carbon sheet tube was estimated as 0.64 by the Monte Carlo Analysis Method. Manufacture and construction process have to be perfectly managed by construction managers because the structural capacities of carbon tubes were depends on construction abilities of manufacturing operators.

• The Transactions of the Korea Information Processing Society
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• v.3 no.2
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• pp.359-368
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• 1996

This paper introduces PGD(Preprocessed Givens Downdating)and PHD(Preprocessed Hyperbolic Downdating) algorithms, wherein a multiple-row observation matrix $Z^T$ is factorized into a partial Cholesky factor Rz, such that $Z^T$ = $Q_zR_z, Q_zQ^T_z=I$, and then Rz is recursively downdated by using GD(Givens Downdating)and HD(Hyperbolic Dondating), respectively. Time complexities of PGD and PHD algorithms are $pn^2$ ＋ $5n^3/6$ 및 $pn^2$＋$n^3/3$ flops, respectively, if p$\geq$n, while those of the existing GD and HD are known to be $5pn^2/2$ and $2pn^2$ flops,, respectively. This concludes that the factorization of observation matrices, which we call preprocessing, would improve the overall performance of the downdating process. Benchmarks on the Sun SPARC/2 system also show that preprocessing would shorten the required downdating times compared to those of downdatings without preprocessing. Furthermore, benchmarks also show that PHD provides better performance than PGD.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.83-89
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• 2001

Recent advances in the structural and molecular biology uncovered that a set of translation factors resembles a tRNA shape and, in one case, even mimics a tRNA function for deciphering the genetic ：ode. Nature must have evolved this 'art' of molecular mimicry between protein and ribonucleic acid using different protein architectures to fulfill the requirement of a ribosome 'machine'. Termination of protein synthesis takes place on the ribosomes as a response to a stop, rather than a sense, codon in the 'decoding' site (A site). Translation termination requires two classes of polypeptide release factors (RFs)： a class-I factor, codon-specific RFs (RFI and RF2 in prokaryotes; eRFI in eukaryotes), and a class-IT factor, non-specific RFs (RF3 in prokaryotes; eRF3 in eukaryotes) that bind guanine nucleotides and stimulate class-I RF activity. The underlying mechanism for translation termination represents a long-standing coding problem of considerable interest since it entails protein-RNA recognition instead of the well-understood codon-anticodon pairing during the mRNA-tRNA interaction. Molecular mimicry between protein and nucleic acid is a novel concept in biology, proposed in 1995 from three crystallographic discoveries, one, on protein-RNA mimicry, and the other two, on protein-DNA mimicry. Nyborg, Clark and colleagues have first described this concept when they solved the crystal structure of elongation factor EF- Tu：GTP：aminoacyl-tRNA ternary complex and found its overall structural similarity with another elongation factor EF-G including the resemblance of part of EF-G to the anticodon stem of tRNA (Nissen et al. 1995). Protein mimicry of DNA has been shown in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex (Mol et al. 1995; Savva and Pear 1995) as well as in the NMR structure of transcription factor TBP-TA $F_{II}$ 230 complex (Liu et al. 1998). Consistent with this discovery, functional mimicry of a major autoantigenic epitope of the human insulin receptor by RNA has been suggested (Doudna et al. 1995) but its nature of mimic is. still largely unknown. The milestone of functional mimicry between protein and nucleic acid has been achieved by the discovery of 'peptide anticodon' that deciphers stop codons in mRNA (Ito et al. 2000). It is surprising that it took 4 decades since the discovery of the genetic code to figure out the basic mechanisms behind the deciphering of its 64 codons.

• Structural Engineering and Mechanics
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• v.6 no.4
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• pp.467-471
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• 1998

A mathematical model is developed to predict the fatigue notch factor of butt welds subject to number of parameters such as weld geometry, residual stresses under dynamic combined loading conditions (tensile and bending). Linear elastic fracture mechanics, finite element analysis, dimensional analysis and superposition approaches are used for the modelling. The predicted results are in good agreement with the available experimental data. As a result, scatters of the fatigue data can be significantly reduced by plotting S-N curve as ($S{\cdot}K_f$) vs. N.