• BMB Reports
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• v.43 no.11
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• pp.732-737
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• 2010

RNA interference is a post-transcriptional silencing mechanism triggered by the bioavailability and/or exogenous introduction of double-stranded RNA (dsRNA) into cells. Here we describe a novel method for the synthesis of siRNA in a single vessel. The method employs in vitro transcription and a single-stranded DNA (ssDNA) template and design, which incorporates upon self-annealing, two promoters, two templates, and three loop regions. Using this method of synthesis we generated efficacious siRNAs designed to silence both exogenous and endogenous genes in mammalian cells. Due to its unique design the single-stranded template is easily amenable to adaptation for attachment to surface platforms for synthesis of siRNAs. A siRNA synthesis platform was generated using a 3' end-biotinylated ssDNA template tethered to a streptavidin coated surface that generates stable siRNAs under multiple cycles of production. Together these data demonstrate a unique and robust method for scalable siRNA synthesis with potential application in RNAi-based array systems.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.201-204
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• 2002

The gellan was extracellular polysaccharide produced by Pseudomonas elodea A TCC 31461 at aerobic condition. Gellan provides various functionalities such as gelling, suspending, stabilizing, emulsifying and binding properties in aqueous systems. In this study, the effect of pH on the cell growth and the gellan production were evaluated in shake- flasks and in 5 ${\ell}$ batch fermentor. In the shake-flasks culture, maximum gellan production was obtained with 1.66g/ ${\ell}$ when initial pH was 7.0. The batch fermentation was performed in the medium pH control ranged pH 5.5-8.5. The maximum gellan production of 1.97g/l was obtained with constant pH 6.0.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.321-324
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• 2002

In situ recovery of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) was performed in transgenic Nicotiana tabacum cell suspension cultures. Aqueous two-phase systems (ATPS) were used to utilize its biocompatibility. Transgenic plant cells could be grown up to 15.7 g/L in normal medium and 18.6 g/L in ATPS composed of 6% (w/w) polyethylene glycol (PEG) 20,000 and 10% (w/w) dextran 2,000,000. It was proved that the A TPS was not harmful to cell growth. In addition, It is expected to recover hGM-CSF simultaneously with cell growth. Using this method, maximum hGM-CSF concentration at 1.64 ng/mL was obtained on day 3.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.121-125
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• 2002

Rhodococcus rhodochrous IGTSS (ATCC 5396S) can break organo sulfur compounds such as dibenzothiophene. Since the environment for biodesulfurization process is invariably hydrophobic, parameters in hydrophobic systems should be examined. For the model oil, hexadecane-containing 5.43mM dibenzothiophene, the volumetric desulfurization rate was decreased with the oil-to-aqueous phase ratio up to 50%. The rate declined sharply after 48h because the cell activity, which is refreshed by medium exchange, was lost. To supply the exhausted nutrients, medium exchange was performed. At 30% oil phase, most of DBT was removed by medium exchange on 48h, and the rate was 2.03mg $DBT_{removed}/L_{dispersion}-hr.$ At 50% oil phase, medium exchange on 60h was performed and the rate was 1.79mg $DBT_{removed}/L_{dispersion}-hr.$ The 300mL flask system was scaled up to a 5-L bioreactor system. On 60 h, a medium exchange was performed and the rate was 5.28mg $DBT_{removed}/L_{dispersion}-hr.$ and all of DBT was removed. It means that we can use the biodesulfurization process even 10 the high oil-to-water phase by some appropriate methods such as controlled feeding of key nutrients and the dilution or removal of some toxic metabolites by continuous reactor.

• KSBB Journal
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• v.24 no.5
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• pp.420-424
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• 2009

The present study was aimed for the fetor in the livestock facilities. Simple detection systems for ammonia and hydrogen sulfide were examined to see the mechanism making the discoloration length of malodorous substance concentration. Detector tube for ammonia showed a good linearity of 99.6% while hydrogen sulfide detector tube gave 99.7% linearity with reproductivity of 95%.

• Journal of Information Processing Systems
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• v.9 no.3
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• pp.405-424
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• 2013

This research paper proposes a secured, robust approach of information security using steganography. It presents two component based LSB (Least Significant Bit) steganography methods for embedding secret data in the least significant bits of blue components and partial green components of random pixel locations in the edges of images. An adaptive LSB based steganography is proposed for embedding data based on the data available in MSB's (Most Significant Bits) of red, green, and blue components of randomly selected pixels across smooth areas. A hybrid feature detection filter is also proposed that performs better to predict edge areas even in noisy conditions. AES (Advanced Encryption Standard) and random pixel embedding is incorporated to provide two-tier security. The experimental results of the proposed approach are better in terms of PSNR and capacity. The comparison analysis of output results with other existing techniques is giving the proposed approach an edge over others. It has been thoroughly tested for various steganalysis attacks like visual analysis, histogram analysis, chi-square, and RS analysis and could sustain all these attacks very well.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.3
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• pp.560-574
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• 2011

In this paper, we propose a hybrid method of Machine Learning (ML) algorithm and a rule-based algorithm to implement a lightweight Named Entity (NE) extraction system for Korean SMS text. NE extraction from Korean SMS text is a challenging theme due to the resource limitation on a mobile phone, corruptions in input text, need for extension to include personal information stored in a mobile phone, and sparsity of training data. The proposed hybrid method retaining the advantages of statistical ML and rule-based algorithms provides fully-automated procedures for the combination of ML approaches and their correction rules using a threshold-based soft decision function. The proposed method is applied to Korean SMS texts to extract person's names as well as location names which are key information in personal appointment management system. Our proposed system achieved 80.53% in F-measure in this domain, superior to those of the conventional ML approaches.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2008.04a
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• pp.103-115
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• 2008

Hemodynamic shear stress, the dragging force generated by blood flow, is known as an anti-atherogenic factor. We tested whether lysyl-tRNA synthetase (KRS) will be utilized as an agent controlling shear-sensing systems. KRS was previously known to be secreted as a pro-inflammatory agent. Here we found that KRS inhibited various shear-stimulated signaling pathways. We further found that KRS binds to detergent-resistant membrane (DRM), indicating that KRS binding molecules exist in DRM, specialized regions of the plasma membrane. DRM plays important roles in a variety of cellular processes and consists of gangliosides, signaling molecules and cytoskeletons. We then determined that KRS was colocalized with integrins ${\alpha}4$, ${\alpha}5$ and $av{\beta}3$. In addition, KRS was shown to be associated with sialic acid, existing at the end of gangliosides. Interestingly, the adherent effect of KRS was inhibited by pretreatment with sialic acid. Moreover, treatment of endothelial cells with neuraminidase appeared to inhibit both the KRS adhesion to endothelial cells and shear-stimulated signaling. In conclusion, KRS is likely to be utilized as a vascular regulator.

• Vacuum Magazine
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• v.2 no.1
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• pp.10-16
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• 2015

With the recognition of nanotechnology as one of the future strategic technologies, the R&D efforts have been performed under exclusive supports of governments and private sectors. At present, nanotechnology is at the focus of research and public attention in almost every advanced country including USA, Japan, and many others in EU. Keeping tracks of such technical trends, center for nanoscale mechatronics and manufacturing (CNMM) was established in 2002 as a part of national nanotechnology promotion policy led by ministry of science and technology (MOST) in Korea. It will hold widespread potential applications in electronics, optical electronics, biotechnology, micro systems, etc, with the promises of commercial visibility and competitiveness. In this paper, wafer scale multilayer nanoimprint lithography technology which is well-known the next generation lithography, roll-typed nanoimprint lithography (R-NIL), roll-typed liquid transfer imprint lithography (R-LTIL), the key technology for nanomanufacturing and nanoscale measurement technology will be introduced. Additionally, its applications and some achievements such as solar cell, biosensor, hard disk drive, and MOSFET, etc by means of the developed multilayer nanoimprint lithography system are introduced.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.124-129
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• 2009

Antioxidant activities of the extract of red seaweed, Polysiphonia morrowii, were evaluated using several in vitro assay systems. Activity-guided fractionation revealed that the 90% MeOH fraction of the P. morrowii extract exhibited the highest antioxidant activity, and that this fraction had a high total phenolic content ($135.7{\pm}5.0\;mg$ gallic acid/g extract). Therefore, the antioxidant activities of the 90% MeOH fraction against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical, reducing power, ferrous chelating, and hydrogen peroxide were investigated. The results revealed that the antioxidant activities of the 90% MeOH fraction were similar and/or superior to that of commercial antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). In addition, the ability of the 90% MeOH fraction to inhibit oxidative damage to DNA was assessed by measuring the conversion of the supercoiled pBR322 plasmid DNA to the open circular form. The 90% MeOH fraction was found to significantly protect this hydroxyl radical-induced DNA damage in a dose-dependent manner. Taken together, these findings suggest that the 90% MeOH fraction of P. morrowii extract and/or its constituents has the potential for use as a new bioresource of antioxidants.