• Animal cells and systems
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• 제2권2호
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• pp.187-193
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• 1998

The four zoeal stages of Alpheus brevicristatus De Haan, 1849 are described and illustrated in detail from larvae roared in the laboratory. The larvae of A. brevicristatus was compared with those of the other Alpheus and a key to the first zoeal larvae of three Korean Alpheus species is provided.

• Animal cells and systems
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• 제9권3호
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• pp.123-125
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• 2005

A new variety, Geranium koraiense var. hallasanense B.-J. Woo & S.-J. Park, is described here from Jeju Island, Korea. The new variety is distributed only in Jeju Island, and readily distinguished from the other varieties of G. koraiense by its shape of radical leaf division, rounded or undulate petal apex, glabrous petal veins, densely pubescent stems, and stylar portion pubescent with trichomes in basal 1/4 portion. A key to the varieties of G. koraiense is provided.

• Animal cells and systems
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• 제7권1호
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• pp.15-24
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• 2003

Early zoeas of snapping shrimps Alpheus lobidens De Haan,1850 and A. sudara Banner and Banner, 1966 are described and illustrated in detail, based on laboratory-reared materials. A provisional key to the known first alpheid zoeas from Korean coast is provided.

• Animal cells and systems
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• 제4권4호
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• pp.305-314
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• 2000

Two new species of Asterocheridae are described from Korean seas: Acontiophorus pilosus associated with a bryozoan of Celeporina sp. in the Sea of Japan and Asteropontius bifurcatus associated with the antipatharian Antipathes japonicus in Jeju Island in Korea Strait. A key to the species of Acontiophorus is provided.

• Animal cells and systems
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• 제16권3호
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• pp.237-244
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• 2012

A taxonomic study of the Palearctic species of the genus Brachyserphus is presented. Two species are newly described as B. acuticaudatus sp. n. and B. semipunctatus sp. n. from South Korea and the Russian Far East. B. striatopropodeatus (Kolyada 1997 syn. n.) is synonymized under B. lucens (Provancher 1883) and a key to the Palearctic species is provided.

• 한국컴퓨터정보학회논문지
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• 제21권3호
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• pp.65-71
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• 2016

To reduce the expenses for development a novel drug, systems biology has been studied actively. Target prediction, a part of systems biology, contributes to finding a new purpose for FDA(Food and Drug Administration) approved drugs and development novel drugs. In this paper, we propose a classification model for predicting novel target genes based on relation between target genes and disease related genes. After collecting known target genes from TTD(Therapeutic Target Database) and disease related genes from OMIM(Online Mendelian Inheritance in Man), we analyzed the effect of target genes on disease related genes based on PPI(Protein-Protein Interactions) network. We focused on the distinguishing characteristics between known target genes and random target genes, and used the characteristics as features for building a classifier. Because our model is constructed using information about only a disease and its known targets, the model can be applied to unusual diseases without similar drugs and diseases, while existing models for finding new drug-disease associations are based on drug-drug similarity and disease-disease similarity. We validated accuracy of the model using LOOCV of ten times and the AUCs were 0.74 on Alzheimer's disease and 0.71 on Breast cancer.

• Bioinformatics and Biosystems
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• 제2권2호
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• pp.46-51
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• 2007

In the proteomics research using mass spectrometry, the protein database search gives the protein information from the peptide sequences that show the best match with the tandem mass spectra. The protein sequence database has been a powerful knowledgebase for this protein identification. However, as we accumulate the protein sequence information in the database, the database size gets to be huge. Now it becomes hard to consider all the protein sequences in the database search because it consumes much computing time. For the high-throughput analysis of the proteome, usually we have used the non-redundant refined database such as IPI human database of European Bioinformatics Institute. While the non-redundant database can supply the search result in high speed, it misses the variation of the protein sequences. In this study, we have concerned the proteomics data in the point of protein similarities and used the network analysis tool to build a new analysis method. This method will be able to save the computing time for the database search and keep the sequence variation to catch the modified peptides.

• Molecules and Cells
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• 제38권11호
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1124-1131
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• 2020

Techniques used for the regulation of gene expression facilitate studies of gene function and treatment of diseases via gene therapy. Many tools have been developed for the regulation of gene expression in mammalian cells. The Lac operon system induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) is one of the employed inducible systems. IPTG mimics the molecular structure of allolactose and has a strong affinity for the corresponding repressor. IPTG is known to rapidly penetrate into mammalian cells and exhibits low toxicity. In the present study, we developed a new inducible expression system that could regulate the expression of genes in mammalian cells using IPTG. Here we confirm that unlike other vector systems based on the Lac operon, this expression system allows regulation of gene expression with lactose in the mammalian cells upon transfection. The co-treatment with IPTG and lactose could improve the regulatory efficiency of the specific target gene expression. The regulation of gene expression with lactose has several benefits. Lactose is safe in humans as compared to other chemical substances and is easily available, making this technique very cost-effective.

• Molecules and Cells
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• 제26권6호
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• pp.616-620
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• 2008

Maintaining redox balance is one of the crucial requirements for a cell to endure stress from the outside. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) plays an important role in the ascorbate-glutathione cycle; one of the major ROS scavenging systems in most known biological systems. A cDNA clone of the DHAR gene from Oryza sativa (OsDHAR) was isolated and overexpressed in Escherichia coli BL21 (DE3) strain from the pET-28a(+) expression vector. The OsDHAR transformed E. coli cells showed significantly higher DHAR activity and a lower level of ROS than the E. coli cells transformed by an empty pET-28a(+) vector. Also, the DHAR-overexpressing E. coli strain was more tolerant to oxidant- and heavy metal-mediated stress conditions than the control E. coli strain. The results suggest that the overexpressed rice DHAR gene effectively functions in a prokaryotic system and provide protection to various oxidative stresses.