• KSBB Journal
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• v.24 no.3
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• pp.227-238
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• 2009

In the recent years, some organic toxic chemicals were used for obtaining high-yield productivity in agriculture. The undegraded pesticides may remain in the agricultural foods through atmosphere, water, and soil and cause public health problems to environmental resources and human beings even at very low concentrations. Small amounts of pesticides can affect a central nervous system, resulting in immunogenic diseases, infertility problems, respiratory diseases and born marrow diseases, which can lead even to death. Monitoring of the environmental pesticide is one of the important issues for the human well-being. Several kinds of biosensors have been successfully applied to the detection of agrichemical toxicity. Also, few platforms for biocide detection have been definitely developed for the degradation and reaction of pesticides. Biochip and electrochemistry experiments involve immobilizing a receptor molecule on a solid substrate surface, and monitoring its interaction with an analyze in a sample solution. Furthermore, nanotechnology can be applied to make high-throughput analyses that are smaller, faster and sensitive than conventional assays. Some nanomaterials or nanofabricated surfaces can be coupled to biomolecules and used in antibody-based assays and enzymatic methods for pesticide residues. The operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in agrichemical defection research and also describe the label-free biosensor for pesticides using various useful detection methods.

• The KIPS Transactions:PartD
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• v.10D no.6
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• pp.935-948
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• 2003

An online scaling method adds new nodes to the shared-nothing database cluster and makes tables be reorganized while the system is running. The objective is to share the workload with many nodes and increase the capacity of cluster systems. The existing online scaling method, however, has two problems. One is the degradation of response time and transactions throughput due to the additional overheads of data transfer and replica's condidtency. The other is and inefficient recovery mechanism in which the overall scaling transaction is aborted by a fault. These problems deteriorate the availability of shared-nothing database cluster. To avoid the additional overheads throughout the scaling period, our scalingmethod consists of twophases : a parallel data transfer phase and a combination phase. The parallel data transferred datausing reduces the size of data transfer by dividing the data into the number of replicas. The combination phase combines the transferred datausing resources of spare nodes. Also, our method reduces the possibility of failure throughout the scaling period and improves the availability of the database cluster.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.7 s.361
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• pp.71-80
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• 2007

This paper describes an efficient hardware design of WiBro security processor (WBSec) supporting for the security sub-layer of WiBro wireless internet system. The WBSec processor, which is based on AES (Advanced Encryption Standard) block cipher algorithm, performs data oncryption/decryption, authentication/integrity, and key encryption/decryption for packet data protection of wireless network. It carries out the modes of ECB, CTR, CBC, CCM and key wrap/unwrap with two AES cores working in parallel. In order to achieve an area-efficient implementation, two design techniques are considered; First, round transformation block within AES core is designed using a shared structure for encryption/decryption. Secondly, SubByte/InvSubByte blocks that require the largest hardware in AES core are implemented using field transformation technique. It results that the gate count of WBSec is reduced by about 25% compared with conventional LUT (Look-Up Table)-based design. The WBSec processor designed in Verilog-HDL has about 22,350 gates, and the estimated throughput is about 16-Mbps at key wrap mode and maximum 213-Mbps at CCM mode, thus it can be used for hardware design of WiBro security system.

• Korean Journal of Acupuncture
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• v.27 no.2
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• pp.95-105
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• 2010

Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on interleukin(IL) production in mouse macrophage stimulatedby lipopolysaccaride(LPS). Methods : Productions of interleukins were measured y High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$(multi-analyte profiling beads) technology. To begin with, cell culture supernatant was obtained after treatment with LPS(1 ${\mu}g/mL$) and SB for 24 hour. Then, it was incubated with the antibody-conj${\mu}g$ated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. After incubating for 30 minutes, Strepavidin-conjugated Phycoerythrin(SAPE) was then added. Incubating for another 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Results : The results of the experiment are as follows. SB significantly inhibited the LPS-induced production of IL-3($9.15{\pm}0.35$ pg/mL) by $6.92{\pm}0.05,\;7.21{\pm}0.11,\;6.96{\pm}0.33,\;and\;7.45{\pm}0.74$ pg/mL at the concentration of 25, 50, 100, and 200 ${\mu}g/mL$ in mouse macrophage RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced production of IL-5($7.30{\pm}0.48$ pg/mL) by $6.50{\pm}0.29,\;6.30{\pm}0.25,\;6.30{\pm}0.25,\;and\;5.80{\pm}0.25$ pg/mL at the concentration of 25, 50 100, and 200 ${\mg}g/mL$ in RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced productiion of IL-9($17.26{\pm}0.19$ pg/mL) by $15.01{\pm}0.43$ pg/mL at the concentration of 25 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced productioh of IL-13($187.80{\pm}2.90$ pg/mL) by $152.80{\pm}4.25,\;172.80{\pm}3.97,\;162.10{\pm}6.67,\;and\;165.30{\pm}11.80$ pg/mL at the concentration fo 25, 50, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-17($18.30{\pm}0.95$ pg/mL) by $13.30{\pm}1.25,\;13.80{\pm}1.11,\;13.30{\pm}0.75,\;and\;14.00{\pm}1.08$ pg/mL at the concentration of 25, 50 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-23($43.90{\pm}0.83$ pg/mL by $39.50{\pm}1.26,\;38.00{\pm}1.78,\;and\;39.60{\pm}2.49$ pg/mL at the concentration of 25, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). Conclusions : These results suggest that SB has anti-inflammatory activity related with its inhibition of IL-3, IL-5, IL-13, IL-17, and IL-23 production in macrophages.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7575-7581
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• 2015

Corticotropin-releasing factor(CRF), one of the stress driven neuropeptides, was widely proposed to influence hair loss and re-growth. For the development of receptor antagonists, the screening system based on intracellular calcium signal process was developed and optimized. The aequorin parental cells were transfected with CRF1 receptor and alpha 16 promiscuous G protein cDNA to establish HEK293a16/hCRF1, a stable cell line for the human CRF1 receptor. In HEK293a16/hCRF1 cells, the range of sauvagine dose response was 12-fold higher($EC_{50}:15.21{\pm}1.83nM$) than in the transiently expressed cells, hence essential conditions for the antagonist screening experiments such as the robust signals and high solvent tolerance were secured. The standard antagonists for the CRF1 receptor, antalarmin and CP154526, resulted $IC_{50}$ values of $414.1{\pm}5.5$ and $290.7{\pm}1.9nM$, respectively. Similar results were presented with frozen HEK293a16/hCRF1 cells. Finally, our HEK293a16/hCRF1 cells with the aequorin based cellular functional assay can be a model system for the development of functional cosmetics and modulators that can have a clinical efficacy on hair re-growth.

• Microbiology and Biotechnology Letters
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• v.42 no.1
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• pp.41-50
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• 2014

In this study, the performances of PVA-encapsulation and non-encapsulation in a fed-batch bioreactor system were compared for biohydrogen production. Hydrogen production in the PVA-encapsulation bioreactor was not significantly different in comparison to the non-encapsulation bioreactor. However, the hydrogen gas in the encapsulation bioreactor could be stably produced when it was exposed to environmental difficulties such as pH impact by the accumulation of organic acids as fermentative metabolic products. Bacterial communities by DGGE analysis were differently shifted between the PVA-encapsulation and non-encapsulation bioreactors from the initial sludge. The community of hydrogen producing bacteria was stable during the experimental period in the PVA-encapsulation bioreactor compared to the non-encapsulation method. The absolute quantitation of the DNA copy number by a high-throughput droplet digital PCR system for six genera contributed to hydrogen production showing that the numbers of dominant bacteria existed at similar levels in the two bioreactors regardless of encapsulation. In both of two bioreactors, not only Clostridium and Enterobacter, which are known as anaerobic hydrogen producing bacteria, but also Firmicutes, Ruminococcus and Escherichia existed with $1{\times}10^5-1{\times}10^6$ copy numbers of ml-samples exhibiting rapid growth during the initial operation period.

• Journal of Internet Computing and Services
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• v.11 no.1
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• pp.35-48
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• 2010

For the next generation mobile communication system, diverse wireless network techniques such as beyond 3G LTE, WiMAX/WiBro, and next generation WLAN etc. are proceeding to the form integrated into the All-IP core network. According to this development, Beyond 3G integrated into heterogeneous wireless access technologies must support the vertical handover and network to be used of several radio networks. However, unified management of each network is demanded since it is individually serviced. Therefore, in order to solve this problem this study is introducing the theory of Common Radio Resource Management (CRRM) based on Generic Link Layer (GLL). This study designs the structure and functions to support the vertical handover and propose the vertical handover algorithm of which policy-based and MCDM are composed between LTE and WLAN systems using GLL and CRRM. Finally, simulation results are presented to show the improved performance over the data throughput, handover success rate and the system service cost.

• KIPS Transactions on Computer and Communication Systems
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• v.10 no.8
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• pp.215-222
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• 2021

In order to alleviate the limited resource problem and interference problem in cellular networks, the dual connectivity technology has been introduced with the cooperation of small cell base stations. In this paper, we design a new efficient and fair resource allocation scheme for the dual connectivity technology. Based on two different bargaining solutions - Generalizing Tempered Aspiration bargaining solution and Gupta and Livne bargaining solution, we develop a two-stage radio resource allocation method. At the first stage, radio resource is divided into two groups, such as real-time and non-real-time data services, by using the Generalizing Tempered Aspiration bargaining solution. At the second stage, the minimum request processing speeds for users in both groups are guaranteed by using the Gupta and Livne bargaining solution. These two-step approach can allocate the 5G radio resource sequentially while maximizing the network system performance. Finally, the performance evaluation confirms that the proposed scheme can get a better performance than other existing protocols in terms of overall system throughput, fairness, and communication failure rate according to an increase in service requests.

• Journal of Wetlands Research
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• v.24 no.2
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• pp.69-82
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• 2022

In this study, we investigated the bacterial community structure in organic rice-fish mixed farming paddy soil by using high-throughput sequencing technology. The results showed that compared with the organic rice cultivated soil, the content of AP (available phosphorus) increased by 310.23 % and the content of OM (organic matter) increased by 168.83%. The most abundant phyla in paddy soils were Proteobacteria, Bacteriodetes, and Chloroflexi, whose relative abundance was above 47.83%. Among the dominant genera, the relative abundance of Limisphaera in paddy soils was observed. Alpha diversity indicated that the bacterial diversity of paddy soils was similar among each other. The bacterial community structure was affected by the relative abundance of bacteria, not the species of bacteria. Principal Coordinated Analysis (PCoA) results showed that the bacterial communities in organic rice-fish mixed farming soil and organic paddy soil were correlated to each other; the bacterial community structure was distinctively grouped by four different systems (paddy soil under organic rice-fish mixed farming system, organic rice cultivation, and conventional rice cultivation), where the first two are closely related to each other than the third one. The results provide basal support for organic agri-cultivation while improving an ecological value at the same time.

• Journal of Internet of Things and Convergence
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• v.10 no.1
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• pp.39-45
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• 2024

Container volume is a very important factor in accurate evaluation of port performance, and accurate prediction of effective port development and operation strategies is essential. However, it is difficult to improve the accuracy of container volume prediction due to rapid changes in the marine industry. To solve this problem, it is necessary to analyze the impact on port performance using the Internet of Things (IoT) and apply it to improve the competitiveness and efficiency of Busan Port. Therefore, this study aims to develop a prediction model for predicting the future container volume of Busan Port, and through this, focuses on improving port productivity and making improved decision-making by port management agencies. In order to predict port container volume, this study introduced the Extreme Gradient Boosting (XGBoost) technique of a machine learning model. XGBoost stands out of its higher accuracy, faster learning and prediction than other algorithms, preventing overfitting, along with providing Feature Importance. Especially, XGBoost can be used directly for regression predictive modelling, which helps improve the accuracy of the volume prediction model presented in previous studies. Through this, this study can accurately and reliably predict container volume by the proposed method with a 4.3% MAPE (Mean absolute percentage error) value, highlighting its high forecasting accuracy. It is believed that the accuracy of Busan container volume can be increased through the methodology presented in this study.