• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.35-50
• /
• 2022

In this review, the compositions of the sex pheromones of 22 lepidopteran species were analyzed and briefly arranged by comparing the research results conducted in different locations worldwide. These compositions can be used as monitoring tools for pests of rice, maize, leguminous crops, perilla, and sesame in Korea. The sex pheromone compounds and those compositions of four species were successfully identified within Korean populations. The optimal compositions of sex pheromones of three other species were selected through behavioral studies using synthetic chemicals of the known pheromone compounds in laboratories and fields, without chemical identification processes in the sex pheromone compounds of Korean populations. For a few species, the roles of the minor components of the sex pheromone volatiles should be re-assessed for use in the development of optimal monitoring lures.

• Genomics & Informatics
• /
• v.12 no.2
• /
• pp.71-75
• /
• 2014

The tRNA structure contains conserved modifications that are responsible for its stability and are involved in the initiation and accuracy of the translation process. tRNA modification enzymes are prevalent in bacteria, archaea, and eukaryotes. tRNA Gm18 methyltransferase (TrmH) and tRNA $m^1G37$ methyltransferase (TrmD) are prevalent and essential enzymes in bacterial populations. TrmH involves itself in methylation process at the 2'-OH group of ribose at the 18th position of guanosine (G) in tRNAs. TrmD methylates the G residue next to the anticodon in selected tRNA subsets. Initially, $m^1G37$ modification was reported to take place on three conserved tRNA subsets ($tRNA^{Arg}$, $tRNA^{Leu}$, $tRNA^{Pro}$); later on, few archaea and eukaryotes organisms revealed that other tRNAs also have the $m^1G37$ modification. The present study reveals Gm18, $m^1G37$ modification, and positions of $m^1G$ that take place next to the anticodon in tRNA sequences. We selected extremophile organisms and attempted to retrieve the $m^1G$ and Gm18 modification bases in tRNA sequences. Results showed that the Gm18 modification G residue occurs in all tRNA subsets except three tRNAs ($tRNA^{Met}$, $tRNA^{Pro}$, $tRNA^{Val}$). Whereas the $m^1G37$ modification base G is formed only on $tRNA^{Arg}$, $tRNA^{Leu}$, $tRNA^{Pro}$, and $tRNA^{His}$, the rest of the tRNAs contain adenine (A) next to the anticodon. Thus, we hypothesize that Gm18 modification and $m^1G$ modification occur irrespective of a G residue in tRNAs.

• Korean journal of applied entomology
• /
• v.28 no.4
• /
• pp.237-243
• /
• 1989

These studies were conducted to investigate the development of chemical resistance in two-spotted spider mite (Tetranychus urticae Koch) through alternate selection for a given period of time with four acaricides including ethion of organophophours compound, dicofol of organo-chlorine compound, cyhexatin of organo-tin compound, and biphenthrin of synthetic pyrethroid compound; and to examine multiple-resistance among the acaricides to the selected populations. The development levels of chemical resistance in the two-spotted spider mite were greatly varied under alternate selection with the different chemical-group combination of four acaricides. Resistant levels of the ethion/dicofol-selected population at 13th-13th generation showed 49.4-fold resistance to ethion and 18.1-fold to dicofol; the ethion/cyhexatin-selected population at 9th-9th generation exhibited 28.1-fold to ethion and 5-fold to cyhexatin; the ethion/biphenthrin-selectd population at 10th-10th generation revealed 39.8-fold to ethion and 19.2-fold to biphenthrin. However, the dicofol/cyhexatin-selected population at 9th-9th generation showed 11.3-fold to dicofol and 4.9-fold to cyhexatin, and the dicofol/biphenthrin-selected population at 12th-12th generation exhibited 11.2-fold to dicofol and 9.4- fold to biphenthrin, while the cyhexatin/biphenthrin-selected population at 9th-9th generation revealed 3.7-fold to cyhexatin and 7.7-fold to biphenthrin. In case of alternate selection, the ehtion/dicofol-selected population revealed moderate multiple-resistance level to biphenthrin. The ethion/cyhexatin-selected and the ethion/biphenthrin-selected populations showed low mutiple-resistance level to biphenthrin and dicofol, respectively. However, the dicofol/cyhexatin-selected, the dicofol/biphenthrin-selected and the cyhexatin/biphenthrin-selected populations exhibited high multiple-resistance level to ethion.

• Food Science and Biotechnology
• /
• v.17 no.3
• /
• pp.564-568
• /
• 2008

The effects of sodium copper chlorophyllin (SCC) on bioaccessibility and uptake of mercury from fish were investigated using an in vitro digestion coupled with a Caco-2 cell. Fish along with SCC was subjected to a simulated in vitro digestion, which simulates both the gastric and small intestinal phase in vivo. Mercury bioaccessibility, the amount of mercury released from fish to aqueous phase following a digestion, was measured. Various amounts of SCC (0.1-25 mg) significantly reduced mercury bioaccessibility in a dose dependent manner by 49-89% compared to the negative control (fish without SCC) (p<0.05). Mercury bioaccessibility in varying molar ratios of mercury to positive control, 2,3-dimercapto-1-propane sulfonate (DMPS) was between 24 and 52%. Mercury uptake by Caco-2 cells from test media containing aqueous phase following in vitro digestion was measured after 6 hr incubation at $37^{\circ}C$. Cellular mercury uptake with increasing amount of SCC ranged from 0.352 to $0.052\;{\mu}g$ mercury/mg protein, while those in DMPS treatment were between 0.14 and $0.27\;{\mu}g$ mercury/mg protein. Our study suggests that SCC can reduce mercury absorption following fish consumption and may be efficient as a synthetic chelating agent for long term chronic mercury exposure in fish eating populations.

• The Bulletin of The Korean Astronomical Society
• /
• v.38 no.2
• /
• pp.75-75
• /
• 2013

Recent observations have shown that some massive globular clusters (GCs) host multiple stellar populations having different heavy element abundances enriched by supernovae. They usually accompany multiple red giant branches (RGBs) in the color-magnitude diagrams (CMDs), and are distinguished from most of the other GCs which display variations only in light element abundances. In order to investigate the star formation histories of these peculiar GCs, we have constructed synthetic CMDs based on the updated versions of Yonsei.Yale ($Y^2$) isochrones and horizontal branch evolutionary tracks which include the cases of enhancements in both helium and the total CNO abundances. To estimate ages and helium abundances of subpopulations in each GC, we have compared our models with the observations on the Hess diagram by employing a ${\chi}^2$ minimization technique. In this talk, we will present our progress in the population modeling for these GCs with multiple RGBs.

• Research in Plant Disease
• /
• v.23 no.4
• /
• pp.295-305
• /
• 2017

This study aimed to investigate the plant growth-promoting and biocontrol potential of a grass culture with Paenibacillus ehimensis KWN8 on tomato. For this experiment, treatments of a chemical fertilizer (F), a bacterial grass culture (G), a 1/3 volume of G plus 2/3 F (GF), and F plus a synthetic fungicide (FSf) were applied to tomato leaves and roots. The result showed that the severity of Alternaria solani and Botrytis cinerea symptoms were significantly reduced after the application of the bacterial grass culture (G and GF) and FSf. In addition, root mortality in G and GF was lower compared to F. Tomato plants treated with G or GF had better vegetative growth and yield compared to F. Application of G affected the fungal and bacterial populations in the soil. In conclusion, treatment with a bacterial grass culture decreased disease severity and increased tomato growth parameters. However, there were no statistically significant correlations between disease occurrence and tomato yields. This experiment presents the possibility to manage diseases of tomato in an environmentally friendly manner and to also increase the yield of tomato by using a grass culture broth containing P. ehimensis KWN38.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.10
• /
• pp.1704-1707
• /
• 2007

Monitoring of the phage-host system of Microlunatus phosphovorus indigenous in activated sludge was attempted. A laboratory-scale activated sludge process was operated for 5 weeks with synthetic wastewater. The phage-host system population in the process was monitored by plaque assay and FISH methods at every 3 days. During the process operation, the phage-host system populations were more or less steady, except for 1 week in the middle of the operation. In that period, initially M. phosphovorus decreased significantly and its lytic bacteriophages increased, and then M. phosphovorus increased back to its original level while its lytic bacteriophages decreased. This observation suggests that lytic bacteriophages should be considered as one of the biological factors affecting the bacterial population dynamics in activated sludge processes.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2017.05a
• /
• pp.81-81
• /
• 2017

Nanotechnology is of great importance to molecular biology and medicine because life processes are maintained by the action of a series of molecular nanomachines in the cell machinery. Recent advances in nanoscale materials that possess emergent physical properties and molecular organization hold great promise to impact human health in the diagnostic and therapeutic arenas. In order to be effective, nanomaterials need to navigate the host biology and traffic to relevant biological structures, such as diseased or pathogenic cells. Moreover, nanoparticles intended for human administration must be designed to interact with, and ideally leverage, a living host environment. Inspired by nature, we use peptides to transfer biological trafficking properties to synthetic nanoparticles to achieve targeted delivery of payloads. In this talk, development of nanoscale materials will be presented with a particular focus on applications to three outstanding health problems: bacterial infection, cancer detection, and traumatic brain injury. A biodegradable nanoparticle carrying a peptide toxin trafficked to the bacterial surface has antimicrobial activity in a pneumonia model. Trafficking of a tumor-homing nanoprobes sensitively detects cancer via a high-contrast time-gated imaging system. A neuron-targeted nanoparticle carrying siRNA traffics to neuronal populations and silences genes in a model of traumatic brain injury. Unique combinations of material properties that can be achieved with nanomaterials provide new opportunities in translational nanomedicine. This framework for constructing nanomaterials that leverage bio-inspired molecules to traffic diagnostic and therapeutic payloads can contribute on better understanding of living systems to solve problems in human health.

• Journal of Life Science
• /
• v.9 no.1
• /
• pp.84-91
• /
• 1999

Sediment samples from the waste-oil spilled sites were screened for microorganisms able to degrade the components of crude oil, and 3 strains that could degrade were obtained. The isolated 3 strains (Xl, X2 and X3) metabolized naphthalene and 2-methyl naphthalene about 80$\%$ as well as hexane and hexadecane about 60~70$\%$ as a sole carbon source in 7 days. The degradation of the waste oil was about 60$\%$. The addition of synthetic surfactant, Triton-X 100 or Tween 20 slightly inhibited the growth of the populations. Xl and X2 were gram negative and X3 was gram positive. Xl and X3 showed ampicillin resistancy. Xl strain having 30kb plasmid has been selected for genetic study. The plasmid was isolated and transformed into E. coli. showing the possibility of the genetically engineered degrader.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.15 no.2
• /
• pp.149-162
• /
• 2012

Habitat loss and fragmentation are ongoing major anthropogenic impacts on landscapes, which can strongly affect ecosystems, populations and species. The studies on forest fragmentation have been progressed, but the studies on definition of forest fragmentation and their synthetic trend in South Korea still leaves much to be desired. Therefore we set the definition of forest fragmentation and reviewed the related papers. We can give a definition that forest fragmentation is not only a process of spatial landscape transformation, but also a process of ecosystem change by it. The trend of studies on forest fragmentation divided into two fields, landscape and ecosystem changes. Forest fragmentation caused by land change altered the composition and configuration of forest patches. Most studies related on that analyzed the change using landscape index. The effects of forest fragmentation on ecosystem subdivided into three fields : biodiversity, edge effects, and invasive species, but the studies in South Korea is short. The study on relations between forest fragmentation and ecosystem change is necessary to face the Convention on Biological Diversity in South Korea. In addition, the fundamental studies on biodiversity is important to mitigate against forest fragmentation.