• Korean Journal of Food Science and Technology
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• v.36 no.4
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• pp.531-536
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• 2004

Synthesis conditions were optimized using response surface methodology for producing structured lipids (SL) by interesterification of DHA-enriched algae oil derived from microalgae, Schizochytrium sp. and corn oil. Reaction was performed fer 24 hr at $55^{\circ}C$ catalyzed by immobilized lipase from Rhizomucor miehei (RM IM) in shaking water bath. Major fatty acids of SL were palmitic (21.70 mol%), oleic (20.20 mol%), and linoleic (27.34 mol%) acids, and DHA (15.06 mol%). To separate newly synthesized SL-triglycerides (TG) species, HPLC with evaporative light scatting detector (ELSD) was used. Production conditions were optimized using central composite design with reaction temperature $(35-75^{\circ}C,\;X_1)$, reaction time $(2-42\;hr,\;X_2)$, and enzyme concentration $(2-14%,\;X_3)$ as variables. When variables were $70.28^{\circ}C\;(X_1),\;28.74\;hr\;(X_2),\;and\;11.30%\;(X_3)$, maximum content of selected three peaks of synthesized SL-TG species was predicted as 6.97 area%.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.9
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• pp.521-527
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• 2016

Nanoscale zero valent iron (nZVI) has been intensively studied for the treatment of a plethora of pollutants through reductive reaction, however, the nano size should be of concern when nZVI is considered for water treatment, due to difficulties in recovery. The loss of nZVI causes not only economical loss, but also potential risk to human health and environment. Thus, the immobilization onto coarse or structured support is essential. In this study, two representative processes for nZVI immobilization on granular activated carbon (GAC) were evaluated, and optimized conditions for synthesizing Fe/GAC composite were suggested. Both total iron content and $Fe_0$ content can be significantly affected by preparation processes, therefore, it was important to avoid oxidation during preparation to achieve higher reduction capacity. Synthesis conditions such as reduction time and existence of intermediate drying step were investigated to improve $Fe_0$ content of Fe/GAC composites. The optimal condition was two hours of $NaBH_4$ reduction without intermediate drying process. The prepared Fe/GAC composite showed synergistic effect of the adsorption capability of the GAC and the degradation capability of the nZVI, which make this composite a very effective material for environmental remediation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.3C
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• pp.271-280
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• 2008

M channel near-perfect-reconstruction(NPR) pseudo-QMF banks are a hybrid of conventional pseudo-QMF design and spectral factorization approach where the analysis and synthesis filters are cosine-modulated versions of the prototype-lowpass filter(p-LPF). However, p-LPF H(z) does not have linear-phase symmetry as well as magnitude-distortion optimization since it is obtained by spectral factorization of $2M^{-th}$ band filter $G(z)=z^{-(N-1)}H(z^{-1})H(z)$. A fair amount of attention, therefore, has been focused on the design of filter banks for reducing only alias-cancellation distortion without reconstructed-amplitude distortion. In this paper, we propose a new method for designing linear-phase p-LPF in NPR pseudo-QMF banks, which is based on Maxflat(maximally flat) FIR filters with closed-form transfer function. In addition, p-LPF H(z) is optimized in this approach so that the 2M-channel overall distortion response represented with $G(z)=H^2(z)$ approximately becomes an unit magnitude response. Through several examples of NPR pseudo-QMF banks, it is shown that the peek ripple of the overall magnitude distortion is less than $3.5{\times}10^{-4}\;({\simeq}-70dB)$ and analysis/synthesis filters have the sharp monotone-stopband attenuation exceeding 100 dB.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.8B
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• pp.1460-1468
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• 2000

The goal of this research is the real-time implementation of MPEG-1 Layer III audio decoder using the fixed-point digital signal processor of TMS320C6201 The main job for this work is twofold: one is to convert floating-point operation in the decoder into fixed-point operation while maintaining the high resolution, and the other is to optimize the program to make it run in real-time with memory size as small as possible. We, especially, devote much time to the descaling module in the decoder for conversion of floating-point operation into fixed-point operation with high accuracy. The inverse modified cosine transform(IMDCT) and synthesis polyphase filter bank modules are optimized in order to reduce the amount of computation and memory size. After the optimization process, in this paper, the implemented decoder uses about 26% of maximum computation capacity of TMS320C6201. The program memory, data ROM, data RAM used in the decoder are about 6.77kwords, 3.13 kwords and 9.94 kwords, respectively. Comparing the PCM output of fixed-point computation with that of floating-point computation, we achieve the signal-to-noise ratio of more than 60 dB. A real-time operation is demonstrated on the PC using the sound I/O and host communication functions in the EVM board.

• Korean Journal of Food Science and Technology
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• v.46 no.3
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• pp.328-333
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• 2014

Folate is a water-soluble vitamin B that is required for the synthesis of amino acids and nucleic acids. It plays an important role in cell division and cell growth in several living organisms. The purpose of this study was to screen strong folate-synthesizing bacteria and to optimize their culture conditions for folate production. Folate production was quantified by microbiological assays by using folate-dependent strain Lactobacillus rhamnosus KCTC 3237. Folate derivatives were identified by LC-MS/MS. Of the 65 strains of bifidobacteria and lactobacilli tested, L. plantarum Fol 708 demonstrated the greatest ability to produce folate. Its optimal pH for folate production was 5.5 in a pH-controlled, lab-scale fermenter. Coculturing L. plantarum Fol 708 with L. brevis GABA 100 in a milk medium enhanced the level of folate produced in comparison to culturing L. plantarum Fol 708 alone.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.36 no.4
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• pp.341-350
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• 2023

The templated grain growth (TGG) method has gained significant attention for its ability to produce highly textured piezoelectric ceramics with significantly enhanced performance, making it a promising method for transducer and actuator applications. However, the texturing process using the TGG method requires the optimization of multiple steps, which can be challenging for beginners in this field. Therefore, in this tutorial, we provide an overview of the TGG method mainly based on our previous published works, including its various processing steps such as synthesizing anisotropic-shaped templates with size and size distribution control using the molten salt synthesis technique, tape casting, and identifying key factors for proper alignment of the templates in the target matrix system. Our goal is to provide a resource that can serve as a basic reference for researchers and engineers looking to improve their understanding and utilization of the TGG method for producing textured piezoelectric ceramics.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.93-99
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• 2011

Amongst a number of potential nonviral vectors, cationic liposomes have been actively researched, with both gemini surfactants and bola amphiphiles reported as being in possession of good structures in terms of cell viability and in vitro transfection. In this study, a cholesterol-based diquaternary ammonium gemini surfactant (Chol-GS) was synthesized and assessed as a novel nonviral gene vector. Chol-GS was synthesized from cholesterol by way of four reaction steps. The optimal efficiency was found to be at a weight ratio of 1:4 of lipid:DOPE (1,2-dioleoyl-L-${\alpha}$- glycero-3-phosphatidylethanolamine), and at a ratio of between 10:1~15:1 of liposome:DNA. The transfection efficiency was compared with commercial liposomes and with Lipofectamine, 1,2-dimyristyloxypropyl-3-dimethylhydroxyethylammonium bromide (DMRIE-C), and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTAP). The results indicate that the efficiency of Chol-GS is greater than that of all the tested commercial liposomes in COS7 and Huh7 cells, and higher than DOTAP and Lipofectamine in A549 cells. Confirmation of these findings was observed through the use of green fluorescent protein expression. Chol-GS exhibited a moderate level of cytotoxicity, at optimum concentrations for efficient transfection, indicating cell viability. Hence, the newly synthesized Chol-GS liposome has the potential of being an excellent nonviral vector for gene delivery.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1183-1190
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• 1999

This paper presents an efficient hardware architecture to improve the frame memory interface occupying the largest hardware area together with motion estimator in implementing MPEG-2 video encoder as an ASIC chip. In this architecture, the memory size for internal data buffering and hardware area for frame memory interface control logic are reduced through the efficient memory map organization of the external SDRAM having dual bank and memory access timing optimization between the video encoder and external SDRAM. In this design, 0.5 m, CMOS, TLM (Triple Layer Metal) standard cells are used as design libraries and VHDL simulator and logic synthesis tools are used for hardware design add verification. The hardware emulator modeled by C-language is exploited for various test vector generation and functional verification. The architecture of the improved frame memory interface occupies about 58% less hardware area than the existing architecture[2-3], and it results in the total hardware area reduction up to 24.3%. Thus, the (act that the frame memory interface influences on the whole area of the video encoder severely is presented as a result.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.12
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• pp.1539-1547
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• 1999

캐리-세이브 가산기 (CSA)는 회로 설계 과정에서 빠른 연산 수행을 위해 가장 널리 이용되는 연산기 중의 하나이다. 그러나, 현재까지 산업체에서 CSA를 이용한 설계는 설계자의 경험에 따른 수작업에 의존하고 있고 그 결과 최적의 회로를 만들기 위해 매우 많은 시간과 노력이 소비되고 있다. 이에 따라 최근 CSA를 기초로 하는 회로 합성 자동화 기법에 대한 연구의 필요성이 대두되고 있는 상황에서, 본 논문은 연산 속도를 최적화하는 효율적인 CSA 할당 알고리즘을 제안한다. 우리는 CSA 할당 문제를 2단계로 접근한다: (1) 연산식의 멀티 비트 입력들만을 고려하여 최소 수행 속도 (optimal-delay)의 CSA 트리를 할당한다; (2) (1)에서 구한 CSA 트리의 수행 속도 증가가 최소화 (minimal increase of delay) 되는 방향으로 CSA들의 캐리 입력 포트들에 나머지 싱글 비트 입력들을 배정한다. 실제 실험에서 우리의 제안된 알고리즘을 적용하여 연산식들의 회로 속도를 회로 면적의 증가 없이 상당한 수준까지 줄일 수 있었다.Abstract Carry-save-adder (CSA) is one of the most widely used implementations for fast arithmetics in industry. However, optimizing arithmetic circuits using CSAs is mostly carried out by the designer manually based on his/her design experience, which is a very time-consuming and error-prone task. To overcome this limitation, in this paper we propose an effective synthesis algorithm for solving the problem of finding an allocation of CSAs with a minimal timing for an arithmetic expression. Specifically, we propose a two step approach: (1) allocating a delay-optimal CSA tree for the multi-bit inputs of the arithmetic expression and (2) determining the assignment of the single-bit inputs to carry inputs of the CSAs which leads to a minimal increase of delay of the CSA tree obtained in step (1). For a number of arithmetic expressions, we found that our approach is very effective, reducing the timing of the circuits significantly without increasing the circuit area.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.439-447
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• 2018

The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at $20^{\circ}C$ in $2{\times}YT$ medium in host E. coli strain ${\Delta}gcvR$ transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% whole-cell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.