• Title/Summary/Keyword: Synthesis

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Arginine Supplementation Recovered the IFN-γ-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2α Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells

  • Xia, Xiaojing;Che, Yanyi;Gao, Yuanyuan;Zhao, Shuang;Ao, Changjin;Yang, Hongjian;Liu, Juxiong;Liu, Guowen;Han, Wenyu;Wang, Yuping;Lei, Liancheng
    • Molecules and Cells
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    • v.39 no.5
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    • pp.410-417
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    • 2016
  • During the lactation cycle of the bovine mammary gland, autophagy is induced in bovine mammary epithelial cells (BMECs) as a cellular homeostasis and survival mechanism. Interferon gamma ($IFN-{\gamma}$) is an important antiproliferative and apoptogenic factor that has been shown to induce autophagy in multiple cell lines in vitro. However, it remains unclear whether $IFN-{\gamma}$ can induce autophagy and whether autophagy affects milk synthesis in BMECs. To understand whether $IFN-{\gamma}$ affects milk synthesis, we isolated and purified primary BMECs and investigated the effect of $IFN-{\gamma}$ on milk synthesis in primary BMECs in vitro. The results showed that $IFN-{\gamma}$ significantly inhibits milk synthesis and that autophagy was clearly induced in primary BMECs in vitro within 24 h. Interestingly, autophagy was observed following $IFN-{\gamma}$ treatment, and the inhibition of autophagy can improve milk protein and milk fat synthesis. Conversely, upregulation of autophagy decreased milk synthesis. Furthermore, mechanistic analysis confirmed that $IFN-{\gamma}$ mediated autophagy by depleting arginine and inhibiting the general control nonderepressible-2 kinase (GCN2)/eukaryotic initiation factor $2{\alpha}$ ($eIF2{\alpha}$) signaling pathway in BMECs. Then, it was found that arginine supplementation could attenuate $IFN-{\gamma}$-induced autophagy and recover milk synthesis to some extent. These findings may not only provide a novel measure for preventing the $IFN-{\gamma}$-induced decrease in milk quality but also a useful therapeutic approach for $IFN-{\gamma}$-associated breast diseases in other animals and humans.

Selection and Analysis of Genomic Sequence-Derived RNA Motifs Binding to C5 Protein

  • Kim, Kwang-sun;Ryoo, Hye-jin;Lee, June-Hyung;Kim, Mee-hyun;Kim, Tae-yeon;Kim, Yool;Han, Kook;Lee, Seol-Hoon;Lee, Young-hoon
    • Bulletin of the Korean Chemical Society
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    • v.27 no.5
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    • pp.699-704
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    • 2006
  • Escherichia coli RNase P is a ribonucleoprotein composed of M1 RNA and C5 protein. Previously, analysis of RNA aptamers selected for C5 protein from a synthetic RNA library showed that C5 protein could bind various RNA molecules as an RNA binding protein. In this study, we searched cellular RNA motifs that could be recognized by C5 protein by a genomic SELEX approach. We found various C5 protein-binding RNA motifs derived from E. coli genomic sequences. Our results suggest that C5 protein interacts with various cellular RNA species in addition to M1 RNA.

THE EFFECT OF NATURAL EXTRACTS ON CELL GROWTH AND CYTOKINE PRODUCTION (생약 추출물이 세포성장 및 cytokine 생산에 미치는 영향)

  • Ryu, In-Cheol;Son, Seong-Heui;Chung, Chong-Pyoung;Bae, Ki-Hwan
    • Journal of Periodontal and Implant Science
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    • v.23 no.1
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    • pp.37-47
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    • 1993
  • The native connective tissue attachment of the periodontium is known to be a complex consisting of gingival fibroblasts, periodontal ligament cells, gingival epithelial cells, cementum, alveolar bone and extensive extracellular matrix (collagen, glycoprotein and proteoglycans). The purpose of this study was to evaluate the effects of natural extracts on DNA, collagen and protein synthesis and inhibition of cytokine production in the gingival and periodontal ligament fibroblasts and gingival epithelial cells. Healthy gingival tissue was obtained from orthodontic treatment patients, and gingival epithelial cells, gingival fibroblasts and periodontal ligament cells were isolated and cultured from the samples. After treated with Ginseng protein, Pluronic F-68, Scutellariae Radix, centella asiatica, PDGF, IGF, DNA synthesis, total protein and collagen synthesis, and cytokine production of gingival epithelial cell, gingival fibroblast and periodontal ligamentcells were measured. MTT method for DNA synthesis, Peterkofsky and Dingerman method for total protein and collagen synthesis, and IL-1 ELISA kit for cytokine production were used. The proliferation of epithelial cells was enhanced in Centella asiatica, Ginseng protein, Pluronic F-68 and Scutellariae Radix. The activities of PDL cells were increased in PDGF, IGF, and Pluronic F-68. Higher collagen synthesis was observed in Scutellariae Radix and total protein synthesis was increased in Scutellariae Radix and PDGF. The inhibitory effects on IL-1, IL-6, $TNF-{\alpha}$ were observed in all exrracts.

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Efficient Design Methodology based on Hybrid Logic Synthesis for SoC (효율적인 SoC 논리합성을 위한 혼합방식의 설계 방법론)

  • Seo, Young-Ho;Kim, Dong-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.3
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    • pp.571-578
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    • 2012
  • In this paper, we propose two main points. The first is the constraint for logic synthesis, and the second is an efficient logic synthesis method. Logic synthesis is a process to obtain the gate-level netlist from RTL (register transfer level) codes using logic mapping and optimization with the specified constraints. The result of logic synthesis is tightly dependent on constraint and logic synthesis method. Since the size and timing can be dramatically changed by these, we should precisely consider them. In this paper, we present the considering items in the process of logic synthesis by using our experience and experimental results. The proposed techniques was applied to a circuit with the hardware resource of about 650K gates. The synthesis time for the hybrid method was reduced by 47% comparing the bottom-up method and It has better timing property about slack than top-down method.

A Study on the A nalysis and Synthesis in Mathematics Education Based on Euclid's 'The Data' and 'On Divisions' (유클리드의 자료론(The Data)과 분할론(On Divisons)에 기초한 수학교육에서 분석과 종합에 대한 고찰)

  • Suh, Bo-Euk
    • Education of Primary School Mathematics
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    • v.14 no.1
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    • pp.27-41
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    • 2011
  • This study is the consideration to 'The Data' and 'On Divisions' of Euclid which is the historical start of analysis and synthesis. 'The Data' and 'On Divisions' compared to Euclid's Elements is not interested. In this study, analysis and synthesis were examined for significance. In this study, means for 'analysis' and 'synthesis' were examined through an analysis of 'The Data' and 'On Divisions'. First, the various terms including analysis and synthesis were examined and the concepts of the terms were analyzed. Then, analysis was divided into 'external analysis' and 'internal analysis'. And synthesis was divided into 'theoretical synthesis' and 'empirical synthesis'. On the basis of this classification problem presented in elementary textbooks and the practical applications were explored.

Style Synthesis of Speech Videos Through Generative Adversarial Neural Networks (적대적 생성 신경망을 통한 얼굴 비디오 스타일 합성 연구)

  • Choi, Hee Jo;Park, Goo Man
    • KIPS Transactions on Software and Data Engineering
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    • v.11 no.11
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    • pp.465-472
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    • 2022
  • In this paper, the style synthesis network is trained to generate style-synthesized video through the style synthesis through training Stylegan and the video synthesis network for video synthesis. In order to improve the point that the gaze or expression does not transfer stably, 3D face restoration technology is applied to control important features such as the pose, gaze, and expression of the head using 3D face information. In addition, by training the discriminators for the dynamics, mouth shape, image, and gaze of the Head2head network, it is possible to create a stable style synthesis video that maintains more probabilities and consistency. Using the FaceForensic dataset and the MetFace dataset, it was confirmed that the performance was increased by converting one video into another video while maintaining the consistent movement of the target face, and generating natural data through video synthesis using 3D face information from the source video's face.

Effect of Orally Administered Branched-chain Amino Acids on Protein Synthesis and Degradation in Rat Skeletal Muscle

  • Yoshizawa, Fumiaki;Nagasawa, Takashi;Sugahara, Kunio
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.133-140
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    • 2005
  • Although amino acids are substrates for the synthesis of proteins and nitrogen-containing compounds, it has become more and more clear over the years that these nutrients are also extremely important as regulators of body protein turnover. The branched-chain amino acids (BCAAs) together or simply leucine alone stimulate protein synthesis and inhibit protein breakdown in skeletal muscle. However, it was only recently that the mechanism(s) involved in the regulation of protein turnover by BCAAs has begun to be defined. The acceleration of protein synthesis by these amino acids seems to occur at the level of peptide chain initiation. Oral administration of leucine to food-deprived rats enhances muscle protein synthesis, in part, through activation of the mRNA binding step of translation initiation. Despite our knowledge of the induction of protein synthesis by BCAAs, there are few studies on the suppression of protein degradation. The recent findings that oral administration of leucine rapidly reduced $N^{\tau}$-methylhistidine (3-methylhistidine; MeHis) release from isolated muscle, an index of myofibrillar protein degradation, indicate that leucine suppresses myofiblilar protein degradation. The details of the molecular mechanism by which leucine inhibits proteolysis is just beginning to be elucidated. The purpose of this report was to review the current understanding of how BCAAs act as regulators of protein turnover.