• Title/Summary/Keyword: Symbiotic fungus

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Microflora Occurring in the Fermentation by Tea Fungus (Tea fungus 발효음료 제조시 발효계의 미생물상)

  • 최미애;최경호;김정옥
    • Journal of Life Science
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    • v.6 no.1
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    • pp.56-65
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    • 1996
  • Black tea extractbsupplemented with 10% sucrose was fermented by fungus at 30$\circ$C. A pellicle thick as 7$\sim$8 mm covered entire surface of the medium and the wxtract converted to acidic beverage(abbreviated below as fermented black tea) by 14 days of fermentation. It was a kind of acetic acid fermentation depending on symbiotic microorganisms. During the fermentation strains of yeasts(Saccharomyces cerevisiae and Eeniella sp.)and bacteria(Bacillus subtilis, Kurthia zopfii, Gluconobacter oxydans and Deinicoccus sp.) were isolated from aqueous layer. Contrastly to it, a bacterial strain(Acetobacter aceti) was isolated from thick pellicle. The bacteria grew as a viscouse cluster on solid agar medium differently from usual strains of A. aceti. Fermented black tea had sweet-sour taste and sweet smell.

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Phylogenetic Relationships of the Mutualistic Fungi Associated with Macrotermes subhyalinus in Oman

  • Hilal S. AlShamakhi;Abdullah M. Al-Sadi;Lyn G. Cook
    • Mycobiology
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    • v.51 no.5
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    • pp.281-287
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    • 2023
  • The symbiotic association between fungus-gardening termites Macrotermes and its fungal symbiont has a moderate degree of specificity-although the symbiotic fungi (Termitomyces) form a monophyletic clade, there is not a one-to-one association between termite species and their fungus-garden associates. Here, we aim to determine the origin and phylogenetic relationships of Termitomyces in Oman. We used sequences of the internal transcribed spacer region (ITS) and the nuclear large subunit ribosomal RNA (LSU rRNA, 25S) gene and analyzed these with sequences of Termitomyces from other geographic areas. We find no evidence for more than a single colonization of Oman by Termitomyces. Unexpectedly, we find Termitomyces in Oman is most closely related to the symbiont of M. subhyalinus in West Africa rather than to those of geographically closer populations in East Africa.

Current Status of Korean Lichen Research -Beginning and Prospect

  • Hur Jae-Seoun;Koh Young Jin
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.16-18
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    • 2003
  • Lichen is one of the most widely distributed eucaryotic organisms in the world. Lichen is the result of a symbiotic association between two unrelated organisms - a fungus and an alga (or cyanobacterium). Researches in Korean lichens were mainly focused on investigation of Korean lichen flora and most of them were primitive and short-term based projects until 1990's. In this communication, current status and prospect of Korean lichen research are briefly discussed with emphasis of KoLRI (Korean Lichen Research Institute) activities and roles in national research projects on bioresource development in Korea.

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Mycelial Properties of Tremella fuciformis and Hypoxylon sp. (흰목이와 Hypoxylon sp.균의 균사적 특성)

  • Chang, Hyun-You
    • The Korean Journal of Mycology
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    • v.26 no.3 s.86
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    • pp.321-326
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    • 1998
  • Two strains, each of Tremella fuciformis and Hypoxylon sp. were isolated and their mycelial properties were investigated. T. fuciformis produced yeast-like conidia and dikaryotic mycelia from white to yellow color. The diameter of the hyphae was $1.5\;to\;3.0\;{\mu}m$, and septa with clamp connections were present. Secondary mycelia with clamp connections could produce fruitbodies on sawdust medium when the environmental conditions were suitable for fructification. The symbiotic fungus, Hypoxylon sp. produced white feather-like mycelia. But the color of old mycelia was changed to light yellow or light brown and pigmented the culture medium from light brown to brown or very dark green. Generally it did not produce conidia, but in a special case it produced conidia of which color was yellow green to grass green and of which shape was sub-elliptical with the size of approximately $3\;to\;5\;{\mu}m$.

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Histological Observation on the Symbiotic Relationships Between Gastrodia elata and Rhizomorph of Armillaria mellea (뽕나무버섯균 균사속과 천마의 공생관계에 대한 조직학적 관찰)

  • Kim, Yong-Kyoo;Km, Myung-Kon;Yoon, Sook;Hong, Jai-Sik
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.41-45
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    • 2000
  • The histological studies were conducted to investigate the symbiotic relationships between Gastrodia elata and Armillaria mellea by using light and electron microscopes. The fungus, A. mellea, penetrated into the cortex of G. elata, in which endomycorrhizal mycelia in the cortical cells appeared to be dissolved and digested, and seemed to be consequently used as nutritional sources for G. elata growth. Staining of infected tissues revealed that protein- and fat-like substances were localized in the cells. The nuclei of cells infected by the fungal mycelia were hypertrophied 1.5 to 2 times as those without the fungal infection.

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The Presence and Importance of VAM Spores in the Soil of Ginseng Gardens

  • Weber, Hans-Christian
    • Proceedings of the Ginseng society Conference
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    • 1998.06a
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    • pp.90-95
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    • 1998
  • Symbiotic associations between land plants and fungi have been known for more than one hundred years. Vesiculararbuscular mycorrhizas (VAM) are the most common symbiosis in flowering plants and can be recognized in almost all plant families. These fungal associations play a very important role in the growth and survival of plant species. However, with respect to the importance and intensity of the VAM, there is great variation among host species. Our knowledge of the VAM fungus-plant association in Araliaceae is very limited. After the first reports of the occurence of VAM in lateral roots of Panax species, mycorrhizal structures are now described as special structures representing the so-called Paris type. In this type, the development of new spores and vesicles is extremely low. This and the type of colonization of the fungus in Panax roots indicates on, one hand, the high intensity of the VAM and, on the other hand, a remarkable dependency for VAM in members of the Panax species. Therefore, it can be easily understood that cultivated Panax plants exhibit a significant uptake of nutrients and this leads to an extremely depleted soil at harvest. Further, the soil is nearly free of the spores of VAM fungi as they germinate each year on the newly developing Panax roots.

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Hepatoprotective and Curative Properties of Kombucha Tea Against Carbon Tetrachloride-Induced Toxicity

  • Murugesan, G.S.;Sathishkumar, M.;Jayabalan, R.;Binupriya, A.R.;Swaminathan, K.;Yun, S.E.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.4
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    • pp.397-402
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    • 2009
  • Kombucha tea(KT) is sugared black tea fermented with a symbiotic culture of acetic acid bacteria and yeasts, which is said to be tea fungus. KT is claimed to have various beneficial effects on human health, but there is very little scientific evidence available in the literature. In the present study, KT along with black tea(BT) and black tea manufactured with tea fungus enzymes(enzyme-processed tea, ET) were evaluated for hepatoprotective and curative properties against $CCl_4$-induced toxicity, using male albino rats as an experimental model by analyzing aspartate transaminase, alanine transaminase, and alkaline phosphatase in plasma and malondialdehyde content in plasma and liver tissues. Histopathological analysis of liver tissue was also included. Results showed that BT, ET, and KT have the potential to revert the $CCl_4$-induced hepatotoxicity. Among the three types of teas tried, KT was found to be more efficient than BT and ET. Antioxidant molecules produced during the fermentation period could be the reason for the efficient hepatoprotective and curative properties of KT against $CCl_4$-induced hepatotoxicity.

Characterization of a Septobasidium sp. Associated with Felt Disease of Schisandra chinensis

  • Choi, In-Young;Lee, Wang-Hyu;Lee, Jong-Jin;Park, Mi-Jeong;Ko, Jeong-Ae;Choi, Jeong-Ran;Shin, Hyeon-Dong
    • Mycobiology
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    • v.44 no.1
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    • pp.58-62
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    • 2016
  • Extensive disease surveys performed during the summers of 2013 and 2014 in Schisandra chinensis orchards resulted in the finding of a Septobasidium sp. associated with felt disease. The fungus was characterized to be symbiotic with a scale insect (Pseudaulacaspis cockerelli). Morphological and molecular characteristics of the Septobasidium isolates were investigated. The isolates were morphologically and phylogenetically close to S. bogoriense. We tentatively describe this isolate as a Septobasidium sp., mainly because of the limited amount of information available on the internal transcribed spacer region of the ribosomal DNA of Septobasidium spp.

Neocosmospora rubicola, an Unrecorded Endophytic Fungus Isolated from Roots of Glycyrrhiza uralensis in Korea

  • Kim, Jin-Hee;Kim, Dong-Yeo;Park, Hyeok;Cho, Jae Hee;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.63-67
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    • 2017
  • Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

Optimal Condition for Mycelial Production of Armillaria mellea (뽕나무버섯(Armillaria mellea) 균사체 생산의 최적화)

  • Kim, Myung-Kon;Choi, Han-Seok;Park, Hyo-Suk;Kim, Sung-Jun
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.187-191
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    • 2003
  • Armillaria mellea, honey mushroom is well known as a symbiotic fungus with Gastodia elata, The mycelial yields of the fungus were compared when cultured with various broth media. The highest yield of cell mass, 2.31 g dry weight/50mL, was obtained on germinated-malt extract broth (GMEB). The optimal broth concentration which was measured hand refractometer for mycelium production was $15\;Brix^{\circ}$. The optimal conditions estimated with response surface methodology under temperature, pH and incubation period were $25.9^{\circ}C$, pH 5.72, 15.22 days, respectively, on GMEB having $15\;Brix^{\circ}$ concentration for mycelial production of A. mellea.