• Title/Summary/Keyword: Swollen surface

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Pre-Infection Behavior of the Pitch Canker Fungus Fusarium circinatum on Pine Stems

  • Thoungchaleun, Vilakon;Kim, Ki-Woo;Lee, Don-Koo;Kim, Chang-Soo;Park, Eun-Woo
    • The Plant Pathology Journal
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    • v.24 no.2
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    • pp.112-117
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    • 2008
  • Pre-infection behavior of Fusarium circinatum on stems of pine species was investigated with scanning electron microscopy. Two-year-old stems of Pinus densiflora and p. rigida were inoculated with the fungal conidial suspension and subjected to $25^{\circ}C$ for up to 16 hr. Most microconidia germinated 12 hr after inoculation on pine stems. Conidia produced germ tubes from either one or both ends of microconidia. Germ tubes grew over the stem surface and appeared to enter host tissues through natural openings on pine stems. Surface cracks in the cork were entrance sites of germ tubes of F. circinatum. In addition, host cell wall cracks were often found at the tip of germ tubes. The cuticle appeared to be eroded either at the tip of germ tubes or around germlings. Germ tubes also produced appressoria-like structures, exhibiting swollen tips of germ tubes on the stem surface. There seems to be no significant differences in the pre-infection behavior of F. circinatum on stems between the two pine species.

Water Lubrication System Supported by High-density Hydrophilic Polymer Brush

  • Kobayashi, Motoyasu;Ishihara, Kazuhiko;Takahara, Atsushi;Suzuki, Atsushi;Kaido, Masataka;Zhe, Wang
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.343-343
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    • 2006
  • Surface-initiated atom transfer radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) was carried out to produce high-density poly(MPC) brush on silicon wafer. Frictional properties of poly(MPC) was investigated by by sliding a glass ball (${\phi}\;10\;nm$) on the substrates over a distance of 20 mm at a sliding velocity of 90 mm/min under loading of 0.49 N at 298 K. Higher friction coefficients were observed in dry N2 atmosphere and in toluene condition, whereas the friction coefficients decreased to 0.02 in humid air and in water. It is supposed that water-swollen poly(MPC) brush works as a lubricant to moderate the interaction between brush and probe.

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Effects of Riser Design and Chemical Composition on the Formation of Shrinkage Cavity in Gray and Ductile Iron Castings

  • Yu, Sung-Kon
    • Journal of Korea Foundry Society
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    • v.24 no.2
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    • pp.101-107
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    • 2004
  • 회주철 및 구상흑연주철에 있어서 압탕방안 및 합금원소가 수축결함의 생성에 미치는 영향을 연구하였다. 두 종류의 압탕방안으로 실린더형상의 계단상시편을 제조하였으며 회주철의 경우 5조성(ISO 150, 200, 250, 300, 350), 구상흑연주철의 경우 6조성(SG 10, 20, 30, 40, 50, 60)을 사용하였다. 회주철 및 구상흑연주칠 공히, 1차 압탕방안의 경우 액상수축에 의한 1차수축결함이 후육부의 표면에 발생하였으며 수축결함의 내면은 매끄러웠다. 회주철의 경우 응고수축에 의한 2차수축결함은 생성되지 않았으나 구상흑연주철의 경우 모든 시편의 내부열점에 2차수축결함이 발생하였고 그 내면은 거칠었다. 2차압탕방안의 경우 회주철의 모든 시편에서는 1차 및 2차수축결함이 발생되지 않았다. 그러나 구상흑연주철의 경우 탄화물 생성원소가 첨가된 SG 40, 50 및 60의 3조성에서 2차수축결함이 열점에 생성되었다. 견고한 ���V주형을 사용하였기 때문에 주형벽이동으로 인한 표면팽창은 어느 경우에도 관찰되지 않았다.

Enhancing the Absorption Properties of Biomass-based Superabsorbent Terpolymer

  • Kim, Jung Soo;Kim, Dong Hyun
    • Elastomers and Composites
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    • v.55 no.4
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    • pp.249-256
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    • 2020
  • Superabsorbent polymers (SAPs) can absorb and retain ten to thousand times their dry mass of water because of their three-dimensional hydrophilic structures. Conventional SAPs are mainly composed of poly(acrylic acid sodium salt) derived from petrochemicals. The present work is aimed at limiting the use of the petrochemical component by replacing it with a biomass-based material. First, the core-SAP was prepared via the terpolymerization of itaconic acid, vinylsulfonic acid, and cellulose, and the optimum conditions in terms of material input ratio were determined. Following this, the core-SAP was surface-crosslinked by esterification with butane diol to improve its liquid permeability and absorbency under load (AUL). The liquid permeability was measured according to the amount of 0.9 wt.% NaCl solution passing between the swollen SAP particles under a given pressure, and the AUL was estimated from the weight of this solution absorbed under 0.3 psi pressure.

Isolation and Identification of Fusicoccum Species from Quercus dentata

  • Kim, Ki Woo;Kim, Pan-Gi;Lee, Myung-Bo
    • Journal of Korean Society of Forest Science
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    • v.96 no.5
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    • pp.515-519
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    • 2007
  • An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to $300{\mu}m$ in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately $8.0{\times}2.7{\mu}m$. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.

Preparation of Silicone Rubber Membrane and its Porosity (Silicone Rubber Membrane의 제조 및 기공특성)

  • Lee, Seung-Bum;Kim, Hyung-Jin;Hong, In-Kwon
    • Elastomers and Composites
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    • v.30 no.3
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    • pp.185-194
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    • 1995
  • Membrane process has been employed to separate a specific substance from gas or liquid mixture, and treat wastewater. This is due to the fact that the substance of mixture can be permeated and separated selectively by membrane. Since Initial equipment and operation costs are not expensive, membrane process has been adopted in various fields such as petroleum Industry, chemistry, polymer, electronics, foods, biochemical industry and wastewater treatment. In this study, $CaCO_3$ particles impregnated in silicone rubber network were extracted by using supercritical carbon dioxide and pore distribution of silicone $rubber-CaCO_3$ was investigated with varying amount of extract. Silicone rubber has excellent mechanical properties such as heat-resistance, cold-resistance etc. and $CaCO_3$ has microporous structure. It is possible to make silicone $rubber-CaCO_3$ composite sheets via work-intensive kneading processes. In so doing $CaCO_3$ particles become distributed and impregnated in silicone rubber network. Supercritical carbon dioxide diffuse through composite sample, then sample is swollen. $CaCO_3$ in silicone rubber network Is dissolved in supercritical carbon dioxide, and its sites become pores. Pore distribution, pore shape and surface area are observed by SEM(scanning electron microscope) micrograph and BET surface area analyzer examination respectively. Pore characteristics of membrane suggest the possibilities that the membrane can be used for process of mixture separation and wastewater treatment.

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Preparation and in vitro Evaluation of a Buoyant Hydrogel Matrix with Hydroxypropylcellulose and Carbopol (히드록시프로필셀룰로오스와 카르보폴을 이용한 부유성 히드로겔 매트릭스의 제조 및 in Vitro 평가)

  • Kim, Sang-Hun;Lee, Min-Suk;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.26 no.2
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    • pp.137-144
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    • 1996
  • The study was carried out for the preparation and evaluation of a buoyant hydrogel matrix (BHM), which is buoyant in a neutral or in pH 2.0 buffer solution, by the aspects of buoyancy, swelling, and drug release. Physical mixtures of HPC and CP in various molar ratio were employed as a mucoadhesive polymer which swells and controls the rate of drug release. Anhydrous citric acid and sodium bicarbonate in the molar ratio of 1:3 were employed as effervescing agents which provide a buoyancy for the mucoadhesive polymeric matrix. The buoyancy in vitro was expressed as both floating time$(T_{fl})$ and surfing time$(T_{sf})$, which are the time required for floating from the bottom to the surface of the medium and the time to keep the floated state at the surface of medium during release studies, respectively. A close relationship was observed between the buoyancy and the amount of effervescing agent added. $T_{fl}$ of the buoyant hydrogel matrices were decreased to about 10 seconds linearly with increasing the amount of effervescing agent in the range of 5 to 15%. $T_{sf}$ of the buoyant hydrogel matrices were varied from 1 to 3 hr depending on the amount of effervescing agent. The swelling was observed by changes in diameter of the buoyant hydrogel matrices in distilled water or acidic buffer solution, resulted in dependences on pH and the amount of effervescing agents. The release of hydrochlorothiazide from the buoyant hydrogel matrices were followed by apparent zero-order kinetics, while the buoyant hydrogel matrices were floated at the surface and maintaining their swollen shapes.

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Comparative study on the external micro-morphology of 3 kinds of minute pollen and spore Herbs (Pini Pollen, Typhae Pollen, Lygodii Spora) utilizing scanning electron microscope (주사전자현미경을 이용한 3종 화분포자류(花粉胞子類) 한약재(송화분(松花粉), 포황(蒲黃), 해금사(海金沙))의 미세형태 비교연구)

  • Kim, Young-Sik;Song, Jun-Ho;Choi, Goya;Lee, Guemsan;Ju, Young-Sung
    • The Korea Journal of Herbology
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    • v.35 no.1
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    • pp.9-18
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    • 2020
  • Objectives : We tried to establish standards for genuine by discriminating 3 kinds of pollen and spore herbs that are highly to be mixed with each other. Using an scanning electron microscope, we prepare a standard for discriminating external morphological characters of minute herbs, thereby contributing to the stable supply of genuine herbal medicines. Methods : Standard samples were confirmed by literature review on external morphological characteristics of original plants and herbal medicines, and collection and identification of original plants. The herbal medicines on the market were purchased and classified with using naked eye observation and magnifier. Finally, micromorphological identifications were conducted using an scanning electron microscope. Results : 1. Pini Pollen was clearly distinguished by its relatively medium size and a pollen grain with two swollen reticulate sacci at both ends. The verrucate ornamentation on the exine surface of the corpus and a sunken leptoma germ pore may be used as a discrimination criteria. 2. Typhae Pollen was distinguished by its relatively small size and a saccus on the end of a pollen grain. Reticulate ornamentation of exine surface of the corpus, and a slightly clear ulcerate germ pore can be used as a discrimination criteria. 3. Lygodii Spora was distinguished by its relatively large triangular-ovate shape and trichotomous fissure. Verrucate-tuberculate ornamentation of exine surface and trilete aperture could be used as a discrimination criteria. Conclusion : These results indicate that the use of electron microscopy is very effective for discriminating the external morphology of minute herbal medicines.

Three-dimensional analysis of the arrangement of microtubules of the outer segment in the ciliary-type photoreceptor cell in the Onchidium dorsal eye

  • Katagiri, Nobuko;Shimatani, Yuichi;Katagiri, Yasuo
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.284-286
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    • 2002
  • The inverted retina of the Onchidium dorsal eye (DE) is composed only of ciliary-type photoreceptor cells (CC's). The outer segment (OS) of the CC is a concentric lamellar structure consisting of many modified ciliary membranes and stains positively with anti-$\beta$-tubulin antibody. Near the base of the OS there are about 30 basal bodies each connecting individually to a cilium. The cilia are rod-shaped at the base, progressing upwards to a flattened sheet-like shape with increasing surface area. Three-dimensional analysis on serial sections demonstrates the ladle-shape of a modified cilium. Many modified cilia wrap around each other like the leaves of a cabbage. Nine pairs of microtubules (MT's) are located regularly in a ring at the base of the cilium, gradually losing their regular arrangement towards the periphery, where they separate into two subgroups that are contained within two swollen portions of a modified cilium. Within the CC of the Onchidium DE, MT's in the modified cilium exist as two poles extending longitudinally in a thin expanded ciliary membrane. This arrangement may support the photoreceptive OS and serve to maintain its structural integrity.

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First Record of Monstrilloid Copepods in Korea: Description of a New Species of the Genus Cymbasoma (Monstrilloida, Monstrillidae)

  • Chang, Cheon-Young
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.2
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    • pp.126-132
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    • 2012
  • A new monstrilloid species belonging to the genus $Cymbasoma$ is described as a preliminary result from nocturnal surveys using a light trap on the east and south coasts of South Korea. Monstrilloid copepods are first recorded in South Korea. $Cymbasoma$ $striifrons$ n. sp. resembles $C.$ $striatum$ (Isaac, 1974) and $C.$ $tumorifrons$ Isaac, 1975 in sharing the character combination of transverse striations on forehead, somewhat small body (generally slightly less or longer than 1 mm), single lobed leg 5 bearing a short medial seta in female, and smooth lateral margin of anal somite without notch and wrinkles. However, $C.$ $striifrons$ n. sp. differs from $C.$ $striatum$ by relatively short and swollen cephalothorax, and rather strongly wrinkled genital somite. $Cymbasoma$ $striifrons$ n. sp. is also distinguished from $C.$ $tumorifrons$ in lacking a rounded protuberance on anterior margin of ventral surface and by relatively short cephalothorax and the number of ventral nipple-like processes on cephalothorax. Herein the new species is described and illustrated, with some comments on the morphological comparison with its allied species.