• Journal of Animal Science and Technology
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• 제49권6호
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• pp.839-846
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• 2007

본 시험은 인삼부산물 급여 유무에 따른 돼지 생산성, 혈액, 도체 및 육질 특성에 관하여 조사하였다. 시험 동물은 2원 교잡종(LandraceYorkshire) 육성－비육돈 24두를 공시하였으며 개시 체중은 65.81±2.02kg이였다. 기초 사료를 대조구(CON)로 설정하였고, 처리구(GBP)은 인삼 부산물을 기초사료의 lupin 2.5%로 대체하였다. 시험구는 완전임의 배치법으로 암·수 동일한 비율로 각 처리구당 4마리씩 3반복 실시하였다. 생산성에서 일당사료섭취량은 GBP가 CON 보다 유의적으로 낮았다(P<0.0001). 혈장의 생화학적 조성에서는 total protein, blood urea nitrogen, glucose, albumin, calcium 그리고 inorganic phosphate는 각각 GBP가 CON 보다 유의적으로 높은 함량을 나타내었다. 도체 및 육질 특성은 GBP 및 CON간에 유의적인 차이가 나타나지 않았다. 돈육의 총 ginsenoside 함량은 GBP가 CON 보다 유의적으로 높은 함량을 나타내었고 TBRAs는 저장 6일과 13일에 GBP가 CON 보다 유의적으로 낮았다. 이상의 시험 결과를 종합하면, 인삼부산물 급여는 혈장의 화학적 조성의 변화를 가져왔고, 돈육의 ginsenoside의 함량을 증가시키고 지방산패도를 감소시켰는데 이 사실은 인삼부산물이 돼지의 사료첨가제로 이용될 수 있는 가능성을 나타낸다.

• 농업과학연구
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• 제45권4호
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• pp.665-675
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• 2018

A total of 80 pigs [(Landrace ${\times}$ Yorkshire) ${\times}$ Duroc] with an average body weight of $72.9{\pm}2.6kg$ were used in the present study to investigate the effects of fermented whole crop wheat and barley with or without supplementing inoculums throughout the restricted feeding in finishing pigs. There were 4 replicate pens per treatment. Pigs were fed ad libitum throughout the experiment as the control (CON), and the other four groups were restricted to 10% in the CON diet and fed ad libitum fermented whole crop cereals: fermented whole crop barley with inoculums; fermented whole crop barley without inoculums; fermented whole crop wheat with inoculums; and fermented whole crop wheat without inoculums. During the entire experiment, the average daily feed intake (ADFI) decreased in the fermented barley and fermented wheat groups compared to the CON, while no difference was observed in the average daily gain (ADG), feed efficiency (gain : feed ratio, G : F) between the control and fermented whole crop barley, wheat diet group. Dry matter and nitrogen digestibility did not show a significant difference among the treatments. In the blood constituents, concentrations of blood urea nitrogen were significantly lower in pigs fed fermented whole crop barley without inoculum diets compared with the other treatments. In conclusion, restricted feeding with fermented whole crop barley and wheat regardless of the supplementing inoculums showed no significant difference in growth performance compared to the CON. This suggests that there is a possibility that fermented whole crop barley and wheat could replace part of the conventional diets.

• 한국산학기술학회논문지
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• 제21권7호
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• pp.277-284
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• 2020

본 실험은 모돈 사료 내 Portulaca oleracea L.(POL)의 첨가가 번식성적, 혈액성상, 면역반응 및 분 중 미생물에 미치는 영향을 구명하기 위해 수행하였다. 본 실험을 위해 총 20두의 모돈(Landrace×Yorkshire; 229.2±1.15 kg)을 공시하였으며, 개시체중에 기반하여, 2처리 10반복, 반복당 1두씩 완전임의 배치하였다. 실험 처리구는 옥수수-대두박 기초사료를 대조구로 하여 기초사료 내 POL을 0.05% 첨가한 처리구가 포함되었으며, 분만 직후부터 포유기간 동안 수행하였다. 사료 내 POL을 첨가하였을 때, 모돈의 체중, 등지방두께 및 번식성적에서는 유의적인 차이가 나타나지 않았다(p>0.05). 혈액성상에서 사료 내 POL의 첨가하였을 때, 모돈의 림프구가 유의적으로 감소하는 것으로 나타났다(p<0.05). 또한, 면역반응에서 사료 내 POL을 첨가하였을 때 포유모돈의 혈 중 IL-1β의 수치는 유의적으로 감소하였으며(p<0.05), IgG는 증가하는 경향이 관찰되었다(p=0.051). 분 중 미생물에서는 POL 처리구에서 포유모돈의 coliforms이 감소하는 경향을 확인하였다(p=0.063). 본 실험의 결과들을 종합해보면, 사료 내 POL의 첨가는 번식성적에 부정적인 영향을 미치지 않으며, 모돈의 혈액성상, 면역반응 및 분 중 미생물에 긍정적인 효과를 미치는 것을 시사한다.

• 대한수의학회지
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• 제47권3호
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• pp.273-279
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• 2007

Swine influenza is an acute, infectious respiratory disease caused by type A influenza viruses in pigs. In the previous studies, serological surveys have indicated the presence of H3N2 swine influenza virus (SIV) since 1995 in Korea. And the percentage of the antibody-positive rate was 39.12% in the survey determining the prevalence of H1N1 SIV antibodies in 2002. The purpose of this study was therefore to investigate the sero-prevalence of SIV regard to the age of the pig and the season between June 2004 and May 2005. In this study, a total of 932 sera were used. These sera were randomly selected from blood samples, which were submitted to Department of Veterinary Pathology, Kangwon National University and Department of Veterinary Virology, Seoul National University from June 2004 to May 2005. These sera have been tested by ELISA test kit (IDEXX Lab, USA) for the SIV H3N2, H1N1 respectively. SAS version 9.1 was used for the statistical analysis based on the age of the pig and the season. The overall sero-prevalence of the antibody against H3N2 SIV was 20.82% (194/932). The overall sero-prevalence of the antibody against H1N1 SIV was 37.23% (347/932). The overall dual sero-prevalence of the antibody against H3N2 and H1N1 SIV was 10.62% (99/932). H3N2 has significant difference in statistically regarding the age of the pig and the season (p<0.0001). H1N1 has significant difference in statistically regarding the age of the pig (p<0.0001) but has not significant difference in statistically regarding the season (p=0.5882).

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1237-1243
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• 2002

We have developed a reliable and noninvasive method for swine genotyping of single locus nuclear gene with aged single hair follicles delivered by general mail. The method is based on booster and nested PCR amplification with step-wise increase of primers and dNTPs concentrations followed by restriction endonuclease digestion. To establish this method, the ryanodine receptor (RYR 1) locus which is an economically important trait in swine industry was employed for genotyping experiment. The 3-step PCR amplication method is much less dependent on the quantity and quality of template DNA and produces enough amplification product for the detection on the ethidium bromide-stained gel such as RFLP analysis. A total of 120 pigs were subjected to the RYR 1 genotyping analysis using three-step PCR method which amplified enough quantity of PCR products from the aged single hair follicles for RFLP analysis and genotyping results were identical to the results of the corresponding ethanol-fixed skeletal muscle tissue. This approach will be a great help for porcine breeders and investigators in genotyping of swine. They can receive genotyping results later by simply plucking single hairs of their pigs at farm and sending them in general mail to the diagnostic laboratory which eliminates the inconveniences to collect ear tissue or blood cells from pigs, or the investigator's need for travel to farms in order to collect fresh hair samples.

• 대한수의학회지
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• 제45권1호
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• pp.55-61
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• 2005

Serum samples of 1,175 pigs from 148 Korean swine farms not using Mycoplasma hyopneumoniae (M. hyo) vaccines were collected for seroepidemiological study of M. hyo infection by indirect ELISA method. Informations of each farm were provided about province where the farm was located and season when blood samples were collected. Then, the selected farms were divided into farm units which had 5 serum samples according to production stages : sow, suckling piglet (<30 days old), nursery pig (30-70 days old), and growing pig (>70 days old). Seroprevalence of M. hyo infection according to production stages, province, and season was investigated by using ELISA-positve rate of the selected samples for each study. This study showed that 85.34% (78.94-91.78%, 95% CI) of farms were positive to M. hyo infection and 34.81% (32.09-37.53%, 95% CI) among pigs were sero-positive to M. hyo infection in Korean swine farms. In the study of seroprevalence by production stage, most farms had sows and growing pigs which were sero-positive to M. hyo infection (sow: 83.05%, growing pigs: 87.72%) and most pigs seemed to be naturally infected by M. hyo at 8-10 weeks of age. Also, M. hyo infection showed seasonal pattern that most pigs were infected in late fall to early winter. However, in the study of seroprevalence by province, there was no significant correlation between province and M. hyo sero-positive rate.

• 대한수의학회지
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• 제10권2호
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• pp.53-57
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• 1970

Hog cholera (HC) virus detection from the artificially infected pigs was made using fluoreescent antibody technique (FAT) and END method. It was observed that the swine origin virulent was detected in most of the organs tested at the early stage of the infection, while the tissue culture attenuated virus was detected only in blood (transitionally), lung, and tonsil.

• 한국임상수의학회지
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• 제21권3호
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• pp.286-290
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• 2004

Cytogenetic and hematological analysis was performed in peripheral blood of pig in the vicinity of Yeonggwang nuclear power station and control area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from pig was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy. MN rates in lymphocytes of pig from Yeonggwang nuclear power station and control area were 10.60/1,000 and 11.10/1,000, respectively. There were no significant differences in MN frequencies and hematological values in pig between Yeonggwang and control area. The study indicates that the MN assay in lymphocyte of pig is a rapid, sensitive and accurate method that can be used to monitor a large population exposed to radiation.

• 동물자원연구
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• 제28권2호
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• pp.56-63
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• 2017

본 연구는 고온기 때 사료 내 다른 에너지 수준 및 비테인 첨가 급여가 육성돈의 영양소 소화율 및 생리학적 변화에 미치는 영향을 구명하기 위해 실시하였다. 실험동물은 삼원교잡종($L{\times}Y{\times}D$; initial body weight, $73.5{\pm}0.5kg$) 거세 수퇘지 12두를 사용하였고 대사틀에 배치하였다. 실험기간은 고온기인 7~8월에 실시하였다. 실험계획은 에너지 2수준(3,300 및 3,400kcal/kg)과 비테인 2수준(0 및 0.5%)이며 $4{\times}4$ Latin square로 하였다. 조단백질 소화율은 고에너지 사료(3,400kcal/kg)가 저에너지 사료보다 유의적으로 높았다(p<0.01). 그러나, 비테인급여는 영양소소화율에 영향을 미치지 않았다. 혈액생화학적 분석 결과에서는 에너지 수준 및 비테인 첨가가 육성돈 내 생리적 변화를 보이지 않았다. 면역반응을 나타내는 혈중 IgG에서는 고에너지 사료가 저에너지사료보다 높았으나(p<0.05) 스트레스 지표를 나타내는 cortisol농도에서는 차이가 나지 않았고, 비테인 첨가급여는 IgG 및 cortisol 농도 변화를 나타내지 않았다. 결론적으로 사료 내 비테인 첨가급여보다 에너지 수준을 높이는 것이 돼지 체내에 더 긍정적인 효과를 보이며, 여름철 고온스트레스를 받는 돼지 사료 내 고에너지를 급여했을 때 어떠한 결과가 나오는지 추후 더 연구해 볼 만한 것으로 사료된다.

• 대한수의학회지
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• 제41권1호
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• pp.89-98
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• 2001

This study was conducted to detect the toxoplasma specific-DNA in circulating blood and organs collected from slaughtered pigs at slaughtering house and experimentally infected pigs with Toxoplasma gondii tachyzoites by polymerase chain reaction(PCR), and also PCR was applied to diagnose for acute phase of swine toxoplasmosis as a newly developed diagnostic test. The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with Tp parasite detection by mouse inoculation(MI). On the other hand, latex agglutination test(LAT) was conducted to detect the serum antibodies comparing with the detection of toxoplasma by PCR and MI. The results obtained were summarized as follows. PCR was able to determine at the lowest level of $10^0/ml$ T. gondii in blood samples which were blended with a serial diluted T gondii in vitro. On the other hand, $10^2/5g$ of T gondii could detect from a variety of tissues including lung, diaphragm, liver, heart, spleen and brain in vitro. The primer was proved to specifically determine T gondii in blood and tissues in vitro but it did not detect Neospora caninum used as a negative control. DNA of T. gondii was effectively extracted by freezing, thawing and grinding twice both tissues mixed with T gondii in vitro and in experimentally infected pig's tissues. PCR detected specific DNA in the blood of experimentally infected pigs at 108 hrs and 120 hrs post-infection, it was the same time that the pigs showed fever and parasitaemia. In case of tissue, specific DNA was, however, detected only lung from experimentally infected pigs. Even though the duration of acute phase was from 3 to 7 days post-infection, but the latex agglutination test (LAT) results appeared from 8 days post-infection. A comparison of sensitivity in determining T gondii in blood samples between PCR and MI, PCR positive rate ranged from 25 to 33.3%, but that of MI covered from 75 to 100%.