• 제목/요약/키워드: Swine Blood

검색결과 128건 처리시간 0.038초

Analysis of in vitro apoptosis induced by virulent Korean isolate of classical swine fever virus in peripheral blood B cell line

  • Kim, Seon-Mi;Lim, Seong-In;Song, Jae-Young;Hyun, Bang-Hun
    • 대한수의학회지
    • /
    • 제52권4호
    • /
    • pp.259-262
    • /
    • 2012
  • Classical swine fever (CSF) is a highly contagious disease among swine that has an important economic impact on worldwide. One clinical symptom of CSF is leukopenia, in particular lymphopenia, which is a characteristic event that occurs early in the course of CSF. Though lymphopenia associated with apoptosis, the pathogenic mechanism underlying the lymphopenia has not been well studied. To understand these mechanisms, we investigated the response of porcine B cell lines to infection with SW03, virulent strain isolated from swine tissue in Korea. This study demonstrated that SW03-infected L35 cell were induced apoptosis through the detection of activated caspase-3. In addition, SW03 infection leaded to alterations in pro-apoptotic, Bax, and anti-apoptotic, Bcl-xL proteins of Bcl-2 family. Our results would suggest that SW03-infected L35 cells induced apoptosis via intrinsic mitochondrial pathway.

돼지인플루엔자바이러스 A형 H3 국내 분리주에 대한 혈청학적 역학조사 (Sero-prevalence against a H3 subtype isolate of swine influenza virus)

  • 김종란;이재영;송대섭;오진식;박봉균
    • 대한수의학회지
    • /
    • 제42권4호
    • /
    • pp.523-529
    • /
    • 2002
  • A Total of 703 swine sera from 55 swine farrns (Mar. 1998 through Feb. 2001) were nation-wide collected for the presence of the antibody to influenza A virus H3 subtype isolate. The presence of antibody was tested by hernagglutination inhibition with chicken red blood cells and seropositiveness was determined by HI titer ${\geq}1$: 40. Sero-prevalence was evaluated based on year, season, region and age, respectively. In consequence, there were seme differences by year, season and region, respectively. High susceptibility was routinely observed in 60 and 90 day-old piglets. Therefore, it seems that the sero-prevalence to swine influenza virus H3 subtype isolate is useful for the prevention and control of swine influenza in Korea.

돼지(자돈)의 성장에 따른 혈액학치 및 혈액화학치에 관한 연구 (Studies on the Hematology and Blood Chemistry of Swine (Piglets) from Birth to Weaning)

  • 박무현;강정부;이효종
    • 대한수의사회지
    • /
    • 제20권12호
    • /
    • pp.756-761
    • /
    • 1984
  • This experiment was conducted to investigate the levels of hematological and blood chemical components in piglets from birth to weaning. Animals used were 8 newborne healthy piglets with about 1.3kg body weight. The number of RBC, Hb concentration, PCV(

  • PDF

Improving behavior characteristics and stress indices of gestating sows housed with group housing facility

  • Jeong, Yongdae;Choi, Yohan;Kim, Doowan;Kim, Joeun;Min, Yejin;Jung, Hyunjung;Kim, Younghwa
    • Journal of Animal Science and Technology
    • /
    • 제62권6호
    • /
    • pp.875-883
    • /
    • 2020
  • This study was conducted to investigate the effects of group-housing facility (GHF), compared to an individual confinement stall (CON), on the reproductive performance, behavior, and stress hormones of gestating sows. A total of 50 primiparous sows (Landrace × Yorkshire) were randomly allocated into either CON (n = 25) or GHF (n = 25) during the gestation period. One week before parturition, the sows were transferred into conventional farrowing crates, and cross-fostering was conducted within 1 d of delivery. Blood was collected for analyses of stress indices at 75 d of gestation and postpartum. Reproductive performance was estimated during the period of birth to weaning. Behavior patterns were identified at 90 d of gestation. Litter size was not different between the CON and GHF treatments. Weaning to estrus interval, however, tended to be lower in the GHF than in the CON (p < 0.1). Activity, treating, belly nosing, and exploring behaviors were observed only in the GHF group, whereas rubbing was shown only with the CON. Serum cortisol concentration was lower in the GHF than in the CON at 75 d of gestation (p < 0.05). Sows housed in the GHF showed lower epinephrine and norepinephrine concentrations than those housed in the CON at postpartum (p < 0.05). The GHF sows demonstrated more natural behavior characteristics associated with stress relief than the CON sows with no adverse effects on reproductive performance. Therefore, these results suggest that GHF could be applied as an alternative housing facility to improve animal welfare on swine farms.

전과정 평가에 의한 양돈 바이오매스의 물질 및 에너지 자원화 잠재량 연구 (Bioenergy and Material Production Potential by Life Cycle Assessment in Swine Waste Biomass)

  • 김승환;김창현;윤영만
    • 한국토양비료학회지
    • /
    • 제44권6호
    • /
    • pp.1245-1251
    • /
    • 2011
  • 본 연구는 축산부문에서 주요한 가축종인 돼지의 사육과 정과 도축 가공과정에서 발생하는 양돈 바이오매스의 발생특성을 조사 분석하고, 전과정 평가 기법을 활용하여 물질(퇴 액비) 및 에너지 (바이오가스) 자원화 잠재량을 평가함으로써 지역단위 바이오매스 순환단지 조성을 위한 기초자료를 확립하고자 하였으며, 이를 위해 양돈 바이오매스의 발생 단계를 사양단계와 도축 가공단계로 구분하여 각각의 단계에서 발생하는 양돈바이오매스의 물질 및 에너지 자원화 잠재량을 평가하였다. 사양단계는 성장단계(사육기간, 평균체중)에 따라 자돈 (1~9주, 23.4 kg), 육성돈 1기 (10~15주, 50 kg), 육성돈 2기 (16~21주, 80 kg), 비육돈 (22~26주, 110 kg)의 단계로 분류하고 도축 가공단계에서 발생하는 혈액과 폐내장류, 장내 잔재물로 구분하여 생산량을 산정하여 양돈 바이오매스의 물질 및 에너지 자원 잠재량을 평가한 결과 돼지 1두에서 발생하는 바이오매스의 총량은 542.02 kg로 나타났다. 양돈 바이오매스는 분 $210.68kg\;head^{-1}$, 뇨 $315.78kg\;head^{-1}$가 발생하는 것으로 평가되었으며, 분뇨 발생량은 성장단계별로 자돈 14.2%, 육성돈 1기 19.6%, 육성돈 2기 30.9%, 비육돈 35.2%를 차지하는 것으로 나타났다. 양돈 바이오매스에서 기인하는 매탄 생산 잠재량은 $24.56Nm^3\;head^{-1}$이였으며, 사양 단계에서 기인하는 메탄 생산 잠재량이 92.9%를 차지하는 것으로 나타났다. BMP 시험에 의한 최대 메탄생산량은 $16.58Nm^3\;head^{-1}$로 나타나 매탄 생산 잠재량의 67.5%가 에너지로 전환 가능하였으며, 94.4%가 사양 단계에서 기인하는 것으로 나타났다.

인천지역에서 발생한 돼지콜레라의 역학적 특성 (Characteristics of outbreak for the classical swine fever (CSF) at Incheon metropolitan area in 2002)

  • 권효정;변재원;이정구;김경호;박은정;이성모;황현순
    • 한국동물위생학회지
    • /
    • 제26권1호
    • /
    • pp.1-9
    • /
    • 2003
  • The aim of this survey was to investigate the characteristics of outbreak farm determined as the classical swine fever(CSF) at Gangwha-gun and Seo-gu, Incheon metropolitan area from October 7 to November 25 in 2002. Sixty pigs in six different farms were confirmed to the CSF and a total of 9,106 pigs containing 3,194 related epidemiologically was slaughtered to stop spreading of the disease. Clinical signs of pigs diagnosed with the CSF were high fever, anorexia, depression, paralysis of hindlimbs, cyanosis, etc and gross lesions were typically represented with hemorrhage of submandibular and superficial lymph node, infarction of spleen, and petechial (ecchymotic) hemorrhage of kidney and skin. But some outbreak farms had not shown remarkable symptoms, so they were confused with other bacterial diseases. White blood cell (WBC) counts, the classical swine fever virus(CSFV) antigen and antibody enzyme linked immunosorbent assay(ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR) results about six farms indicated that total 60 pigs were infected with the CSFV. Although the origin and infection route of the CSFV were not clear, but the transmissions between farms were mainly through indirect contact such as the movement of farm personal and vehicles from outbreak farm.

Toxoplasma gondii의 조직배양(組織培養)에 관한 연구(硏究) I 돼지의 Buffy Coat Cell Culture에서의 증식소견(增植所見) (Studies on the Toxoplama gondii in Buffy Coat Cell Culture I. Multiplication of Toxoplasma gondii in Swine Origin Buffy Coat Cells)

  • 김종면
    • 대한수의학회지
    • /
    • 제13권1호
    • /
    • pp.67-73
    • /
    • 1973
  • Toxoplasma gondii (Tp), RH strain, was inoculated into cultured buffy coat cells obtained from the swine blood. The main reason for adopting swine lies in the animal's unusual susceptibility to Tp, As for the culture method used in the experiment, those well proved methods practised by Cho, Merchant, Moore and Tarnvik were mainly referred to as a starting point: hence, the author's method has been turned out to be the modified or supplementary form of those methods. Observations were made on the phase of multiplication of Tp in the cytoplasm. The results obtained were as follows: 1. Better growth and multiplication of Toxoplasma gondii were noticeably observed in the swine buffy coat cell, inoculated after three-to-five day cultivation of the cell. 2. In the lapse of the observation period, there appeard Toxoplasma gondii rarely available in the earlier stage, which had been inoculated into the cell after three-to-five day cultivation. In other words, Toxoplasma gondii started to show itself in seven or eight hours after inoculation, most outstandingly noticeable between twenty four hours and forty eight hours. Thereafter the disintegration stage of Toxoplasma gondii was observed.

  • PDF

Seroprevalence of swine influenza and porcine reproductive and respiratory syndrome in Korea

  • Jeong, Kwang;Park, Young-Il;Jin, Wen;Han, Jeong-Hee
    • 한국동물위생학회지
    • /
    • 제30권2호
    • /
    • pp.197-203
    • /
    • 2007
  • A total of 501 serum samples were selected from blood samples that were submitted to Department of Veterinary Pathology, Kangwon National University from all provinces in Korea from September 2001 to August 2002. Their sera were examined for antibodies to swine influenza virus subtype H1N1 (SlV H1N1) and porcine repro-ductive and respiratory syndrome virus (PRRSV) according to the age of pig, season, and herd size using enzyme-linked immunosorbent assay. The seroprevalence of SIV H1N1, PRRSV, and dual infection were 39.12%, 61.48%, and 25.95%, respectively. The seroprevalence of SIV H1N1 according to herd size was not significant differences (p>0.05). The results showed that the PRRSV infection spread widely in swine herds throughout the country.

수송시간이 돼지의 혈액성상과 육질에 미치는 영향 (Effect of Transport Time on the Blood Profile and Meat Quility of Slaughter Pigs)

  • 이제룡;서종태;허태영;정재두;하영주;이진우;이정일;이중동
    • Journal of Animal Science and Technology
    • /
    • 제45권5호
    • /
    • pp.857-864
    • /
    • 2003
  • 여름철 수송시간이 돼지의 혈액성상 및 돈육품질에 미치는 영향을 구명하고자, 생체중 110kg 내외의 비육돈 120두를 공시하여 실험하였다. 수송시간은 농가에서 도축장까지 20분과 2시간으로 하였고 계류는 약 1시간 30분 실시하였다. 돼지 혈액은 도축장 하차직후 채취하였다. 관행적인 방법으로 도축 후 도체특성을 조사하였다. 도축 1시간 후 pH$_1$과 도체의 피부손상을 측정한 후, 도체를 냉각시키고 사후 24시간에 최종 pH$_{u}$ 및 돈육품질을 조사하였다. 20분과 2시간 수송사이 도체중, 등지방두께 및 도체등급은 차이를 나타내지 않았다. 돼지의 심리적 스트레스 수준을 측정하는 Cortisol과 물리적 스트레스 수준은 측정하는 LDH는 2시간 수송이 20분 수송에 비해 유의하게(P〈0.05) 높게 나타났다. 돈육의 이화학적 특성 중 pH$_{u}$는 20분 수송이 높은 경향을 나타냈고, 가열 감량, 육즙 감량 및 경도는 차이를 나타내지 않았다. CIE L$^{*}$, a$^{*}$ b$^{*}$와 주관적인 육색인 NPPC에도 수송시간에 의해 영향을 받지 않았고 또한 도체의 피부손상도 차이가 인정되지 않았다. 이상의 결과, 돈육품질은 현저한 차이를 보이지 않았지만 2시간 수송한 돼지가 스트레스를 많이 받는 것으로 나타났다.

배양산삼 분말 및 그 발효산삼배양액 분말 첨가가 비육돈의 생산성 및 도체 특성에 미치는 영향 (Effects of Dietary Supplementation of Cultured Wild-ginseng Powder or its Fermented Culture Byproducts on Growth Performance and Carcass Parameters in Finishing Pigs)

  • 박준철;김영화;정현정;이성대;장해동;김인철;이상진;이재정;이찬호;이상석
    • Journal of Animal Science and Technology
    • /
    • 제48권6호
    • /
    • pp.819-826
    • /
    • 2006
  • 본 시험은 발효산삼배양액 분말 및 배양산삼 분말 급여에 따른 돼지 생산성과 혈액, 도체 및 육질 특성에 관하여 조사하였다. 시험동물은 2원 교잡종(Landrace×Yorkshire) 육성비육돈 36두를 공시하였으며, 개시체중은 65.81±2.02kg 이였다. 시험사료는 기초사료를 대조구로 설정하였고, 처리구 1(T1)은 기초사료의 lupin 2.5%를 발효산삼배양액 분말로 배합하였고, 처리구 2(T2)는 기초사료의 lupin 0.2%를 배양산삼 분말로 배합하였다. 시험구는 암수 동일한 비율로 각 처리구당 4마리씩 3반복으로 완전임의 배치하여 실시하였다. 생산성에서 일당 증체량은 유의적인 차이가 나타내지 않았다. 사료섭취량은 대조구보다 처리구인 T1과 T2가 유의적으로 낮았다(P<0.05). 사료요구율은 시험구간에 유의적인 차이가 없었다.혈장의 생화학적 조성에서 total protein은 T1이 대조구보다 유의적으로 높았다(P<0.05). Blood urea nitrogen은 시험구간에 유의적인 차이는 없었다. Glucose와 albumin은 T1이 다른 시험구에 비하여 유의적으로 높았다(P<0.05). Calcium은 T1이 대조구보다 유의적으로 높았고(P<0.05), inorganic phosphate는 T1이 다른 시험구에 비하여 유의적으로 높았다(P<0.05).혈장의 지질 구성물의 조성에 있어서 triglyceride는 T1이 다른 시험구에 비하여 유의적으로 높았다(P<0.05). Total cholesterol은 시험구간에 유의적인 차이는 없었으나, HDL cholesterol은 T1이 대조구에 비하여 유의적으로 높았다(P<0.05).도체 및 육질 특성에서 도체중량, 도체율, 정육율, 등지방두께는 시험구간에 유의적인 차이가 없었다. 그리고 고기내 수분과 조지방도 시험구간에 유의적인 차이는 나타내지 않았다.이상의 결과를 종합하면, 발효산삼배양액 분말 및 배양산삼 분말 급여는 돼지의 생산성, 도체 및 육질 특성에는 영향을 주지 않았고, 혈장의 구성물질의 변화에 영향을 주었다. 따라서 본 연구에 이용한 발효산삼배양액 분말 및 배양산삼 분말은 돼지 사료 원료로서 이용 가능성을 확인할 수 있었다.