• Title/Summary/Keyword: Swarming

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Mutation in clpxoo4158 Reduces Virulence and Resistance to Oxidative Stress in Xanthomonas oryzae pv. oryzae KACC10859

  • Cho, Jung-Hee;Jeong, Kyu-Sik;Han, Jong-Woo;Kim, Woo-Jae;Cha, Jae-Soon
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.89-92
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    • 2011
  • Cyclic AMP receptor-like protein (Clp), is known to be a global transcriptional regulator for the expression of virulence factors in Xanthomonas campestris pv. campestris (Xcc). Sequence analysis showed that Xanthomonas oryzae pv. oryzae (Xoo) contains a gene that is strongly homologous to the Xcc clp. In order to determine the role of the Clp homolog in Xoo, a marker exchange mutant of $clp_{xoo4158}$ was generated. Virulence and virulence factors, such as the production of cellulase, xylanase, and extracellular polysaccharides (EPS) and swarming motility were significantly decreased in the $clp_{xoo4158}$ mutant. Moreover, the mutation caused the strain to be more sensitive to hydrogen peroxide and to over-produce siderophores. Complementation of the mutant restored the mutation-related phenotypes. Expression of $clp_{xoo4158}$, assessed by reverse-transcription realtime PCR and clp promoter activity, was significantly reduced in the rpfB, rpfF, rpfC, and rpfG mutants. These results suggest that the clp homolog, $clp_{xoo4158}$, is involved in the control of virulence and resistance against oxidative stress, and that expression of the gene is controlled by RpfC and RpfG through a diffusible signal factor (DSF) signal in Xanthomonas oryzae pv. oryzae KACC10859.

PI-based Containment Control for Multi-agent Systems with Input Saturations (입력 포화가 존재하는 다중 에이전트 시스템을 위한 PI기반의 봉쇄제어)

  • Lim, Young-Hun;Tack, Han-Ho;Kang, Shin-Chul
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.1
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    • pp.102-107
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    • 2021
  • This paper discusses the containment control problem for multi-agent systems with input saturations. The goal of the containment control is to obtain swarming behavior by driving follower agents into the convex hull which is spanned by multiple leader agents. This paper considers multiple leader agents moving at the same constant speed. Then, to solve the containment problem for moving leaders, we propose a PI-based distributed control algorithm. We next analyze the convergence of follower agents to the desired positions. Specifically, we apply the integral-type Lyapunov function to take into account the saturation nonlinearity. Then, based on Lasalle's Invariance Principle, we show that the asymptotic convergence of error states to zero for any positive constant gains. Finally, numerical examples with the static and moving leaders are provided to validate the theoretical results.

Duplex Control for Consensus of Multi-agent Systems with Input Saturations (입력포화가 존재하는 다중 에이전트 시스템의 일치를 위한 이종제어)

  • Lim, Young-Hun
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.14 no.4
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    • pp.284-291
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    • 2021
  • In this paper, we study the consensus problem for multi-agent systems with input saturations. The goal of consensus is to achieve a swarming behavior of multi-agent systems by reaching the agreement through information exchange. This paper considers agents modeled by first-order dynamics with input saturations. In order to guarantee the global convergence of the agents, it is assumed that the agents are stable. Moreover, considering the disturbances, we propose the PI based duplex control method to achieve the consensus. The proposed P controller and I controller are composed of different information network. Then, we investigate the conditions of the information networks and the control gains of P, I controllers to achieve the consensus applying the Lyapunov stability theorem and the Lasalle's Invariance Principle. Finally, we conduct the simulations to validate the theoretical results.

A Case Study on FPV Drone Combats of the Ukrainian Forces (우크라이나군의 FPV드론 전투 사례 연구)

  • Kang-Il Seo;Sang-Keun Cho;Sang-Hyuk Park
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.3
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    • pp.263-270
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    • 2023
  • The Ukraine-Russia war is a testing ground for High-Tech weapons and the first full-scale drone warfare. The Ukrainian military has been aggressively deploying a variety of reconnaissance and attack drones on the battlefield, and more recently, FPV drones, also known as racing drones, have been fitted with bombs as a game-changer in small-unit combat. To better understand these FPV drones, this article reviews their definition, aircraft components, and key characteristics, and draws implications from the Ukrainian military's FPV combat experience. In the future, the combination of artificial intelligence and swarming technology will make FPV drones even more lethal. Accordingly, the need to develop FPV drones suitable for the future operational environment on the Korean Peninsula is increasing, and follow-up research is needed to specify fighting methods and optimize related technologies.

Research on Vocalization for Korean Pop Song: Case Foom The YongPhil Cho's "A Lady out of Window" (대중가요 발성법 연구: 조용필의창밖의 여자중심으로)

  • Cho, Tae-Seon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.3004-3010
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    • 2009
  • The Korean pop music people enjoy today is mostly based on the pop music from foreign countries. Therefore, not only is the history very short, but also not much research is done in the Korean pop music field. Especially in terms related with the singing method, in other words the breathing and vocalization of Korean singers, it is difficult to define where it is actually situated. To add a little, it seems as if it is swarming somewhere in the middle of Korean traditional music singing methods and classical music vocalization. As pop music made its way into Korea, Korean singers had to go through many trial and errors to sing the songs of singers that had different physical conditions. Singers getting vocal nodules because of excessive vocalization, was not a surprise at that time. Yongphil Jo, a Korean singer, wanted to overcome his limits and maximize his vocal abilities. He found the answers in Korean traditional music. One of his representative songs, "The lady out the window", is one of Jo's title songs and it is the song that made a turning point in the Korean pop music vocalization. The purpose of this research is to understand the circumstances of Korean pop music through the periodical and musical background of the past and find out why the song (The lady out the window) was such an epochal vocal method at that time. Moreover, this paper covers how the breathing and vocalization is expressed by analyzing the flow, the emotional expressions, and the techniques of "The lady out the window."

Interaction of Pseudostellaria heterophylla with Quorum Sensing and Quorum Quenching Bacteria Mediated by Root Exudates in a Consecutive Monoculture System

  • Zhang, Liaoyuan;Guo, Zewang;Gao, Huifang;Peng, Xiaoqian;Li, Yongyu;Sun, Shujing;Lee, Jung-Kul;Lin, Wenxiong
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2159-2170
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    • 2016
  • Many plant-pathogenic bacteria are dependent on quorum sensing (QS) to evoke disease. In this study, the population of QS and quorum quenching (QQ) bacteria was analyzed in a consecutive monoculture system of Pseudostellaria heterophylla. The isolated QS strains were identified as Serratia marcescens with SwrIR-type QS system and exhibited a significant increase over the years of monoculture. Only one QQ strain was isolated from newly planted soil sample and was identified as Bacillus thuringiensis, which secreted lactonase to degrade QS signal molecules. Inoculation of S. marcescens to P. heterophylla root could rapidly cause wilt disease, which was alleviated by B. thuringiensis. Furthermore, the expression of lactonase encoded by the aiiA gene in S. marcescens resulted in reduction of its pathogenicity, implying that the toxic effect of S. marcescens on the seedlings was QS-regulated. Meanwhile, excess lactonase in S. marcescens led to reduction in antibacterial substances, exoenzymes, and swarming motility, which might contribute to pathogensis on the seedlings. Root exudates and root tuber extracts of P. heterophylla significantly promoted the growth of S. marcescens, whereas a slight increase of B. thuringiensis was observed in both samples. These results demonstrated that QS-regulated behaviors in S. marcescens mediated by root exudates played an important role in replanting diseases of P. heterophylla.

Development and Commercialization of Artificial Reefs from Waste Mulch Plastic Films (농업용(農業用) 멀칭 폐(廢)비닐로부터 인공어초(人工魚礁) 개발(開發) 및 상용화(商用化))

  • Kim, Hea-Tae;Shon, Sang-Jin;Kim, Myoung-Ho;Choi, Mi-Ran;Baek, Wook-Jin
    • Resources Recycling
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    • v.16 no.5
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    • pp.19-24
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    • 2007
  • Reefs are the marine structure that can give resting, inhabiting, feeding and nursing spaces for a variety of fishes. Usually artificial reefs are made of cement and steels respectively in Japan as well as in Korea. However since resources deficiency has been getting serious, other materials are taken into consideration for the basic bodies of artificial reefs. About 300 thousand tons of waste agricultural plastic films are generated every yew in Korea, but no effective recycling techniques have been developed. In this sense, artificial reefs made of waste agricultural plastic films are the most representative symbol of the recycled products in the Resource Recycling Era. In particular, since these reefs could be made of the semi-cleaned waste agricultural plastic films that still contain high portion of soil, it is very environmentally friendly not only in manufacturing process but also in using under water. Furthermore they have some evident advantages as follows; 1) high fish swarming effect 2) good initial attachment of the marine growths 3) extremely low corrosion to brine 4) easy adjustment of the gravity 5) economical manufacture, transportation and jettison 6) excellent safety to ecosystem caused by lower elution of toxic substances 7) good recyclable property after application and so on.

A study on the honeycomb entry and exit counting system for measuring the amount of movement of honeybees inside the beehive (벌통 내부 꿀벌 이동량 측정을 위한 벌집 입·출입 계수 시스템 연구)

  • Kim, Joon Ho;Seo, Hee;Han, Wook;Chung, Wonki
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.857-862
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    • 2021
  • Recently, rapid climate change has had a significant impact on the bee ecosystem. The decrease in the number of bees and the change in the flowering period have a huge impact on the harvesting of beekeepers. Accordingly, attention is focused on smart beekeeping, which introduces IoT technology to beekeeping. According to the characteristics of beekeeping, it is impossible to continuously observe the beehive in the hive with the naked eye, and the condition of the hive is mostly dependent on knowledge from experience. Although a system that can measure partly through sensors such as temperature/humidity change inside the hive and measurement of the amount of CO2 is applied, there is no research on measuring the movement path and amount of movement of bees inside the beehive. Part of the migration of honeybees inside the hive can provide basic information to predict the most important cleavage time in beekeeping. In this study, we propose a device that detects the movement path of bees and measures and records data entering and exiting the hive in real time. The device proposed in this study was developed according to the honeycomb standard of the existing beehive so that beekeeping farms could use it. The development method used a photodetector that can detect the movement of bees to configure 16 movement paths and to detect the movement of bees in real time. If the measured honeybee movement status is utilized, the problem of directly observing the colony with the naked eye in order not to miss the swarming time can be solved.