• Rapid Communication in Photoscience
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• v.3 no.3
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• pp.48-49
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• 2014

For in vitro myelination system, Schwann cells and neuronal cells of rat were cocultured. Schwann cells and neuronal cells, respectively, were obtained from dorsal root ganglion of rat embryos (E15). This method includes four steps: first step of suspension of the embryonic dorsal root ganglion cells, second step of addition of anti-mitotic cocktail, third step of purification of dorsal root cells, and fourth step of addition of Schwann cells to dorsal root ganglion cells. We made a highly purified population of myelination in a short period through this procedure and identified myelination basic protein using antibody of myelination basic protein.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.3
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• pp.161-166
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• 2003

In order to improve the fatigue lift of a lower control arm in the vehicle suspension, a new shape optimization procedure is presented. In this approach, the shape control point concept is introduced to reduce the numbers of shape design variables. Also, the two-level orthogonal way is employed to evaluate the design sensitivity of fatigue life with respect to those shape design variables, because the analytical design sensitivity information is not directly supplied from the commercial CAE softwares. In this approach, only the six design variables are used to approximate the shape of lower control arm. Then, performed are only 10 fatigue life analyses including the baseline design, 8 DOE models and the final design. The final design, the best combination obtained from the sensitivity information, can maximize the fatigue lift nearly two times as that of the baseline design, while reducing the 12 percentage of weight than it.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.5
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• pp.48-55
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• 2007

A bushing component of a vehicle suspension system is tested to capture the nonlinear behavior of rubber bushing element using the MTS 3-axes rubber test machine. The results of the tests are used to model the artificial neural network bushing model. The performances from the neural network model usually are dependent on the structure of the neural network. In this paper, maximum error, peak error, root mean square error, and error-to-signal ratio are employed to evaluate the performances of the neural network bushing model. A simple simulation is carried out to show the usefulness of the developed procedure.

• Journal of the Korean Society for Railway
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• v.10 no.2 s.39
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• pp.211-216
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• 2007

Rubber components are widely used in many application such as vibration isolators, damping, ride quality. Rubber spring is used in primary suspension system for railway vehicle. Characteristics and useful life prediction of rubber spring was very important in design procedure to assure the safety and reliability. Non-linear properties of rubber material which are described as strain energy function are important parameter to design and evaluate of rubber spring. These are determined by physical tests which are uniaxial tension, equi-biaxial tension and pure shear test. The computer simulation was executed to predict and evaluate the load capacity and stiffness for rubber spring. In order to investigate the useful life, the acceleration test were carried out. Acceleration test results changes as the threshold are used for assessment of the useful life and time to threshold value were plotted against reciprocal of absolute temperature to give the Arrhenius plot. By using the acceleration test, several useful life prediction for rubber spring were proposed.

• International Journal of Vascular Biomedical Engineering
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• v.4 no.1
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• pp.17-26
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• 2006

Diabetes mellitus (DM) is a metabolic disorder, characterized by varying or persistent hyperglycemia, which induces several changes in the erythrocyte membrane and its cytoplasm, leading to alteration in the deformability. Techniques applied to measure this are based on filtration of erythrocyte suspension through a membrane and to obtain diffraction pattern under sheared conditions. Ektacytometry requiring less quantity of blood with disposable flow chamber used to measure the deformability of erythrocytes obtained from patients with diabetes and also associated with nephropathy and retinopathy. A decreasing trend of deformability in these patients is observed. The shape parameter form factor, as determined by image processing procedure, increases with the increased of blood glucose levels and shows a pattern similar to filtration time of erythrocyte suspensions through cellulose membranes. Further work is suggested to detect micro-level changes in cell membrane in diabetic patients

• Wind and Structures
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• v.5 no.6
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• pp.479-492
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• 2002

A finite element aerodynamic model that can be used to analyse flutter instability of long span bridges in the time domain is presented. This approach adopts a simplified quasi-steady formulation of the wind forces neglecting the vortex shedding effects. The governing equations used are effective only for reduced velocities $V^*$ sufficiently great: this is generally acceptable for long-span suspension bridges and, then, the dependence of the wind forces expressions of the flutter derivatives can be neglected. The procedure describes the mechanical response in an accurate way, taking into account the non-linear geometry effects (large displacements and large strains) and considering also the compressed locked coil strands instability. The time-dependence of the inertia force due to fluid structure interaction is not considered. The numerical examples are performed on the three-dimensional finite element model of the Great Belt East Bridge (DK). A mode frequency analysis is carried out to validate the model and the results show good agreement with the experimental measurements of the full bridge aeroelastic model in the wind tunnel tests. Significant parameters affecting bridge response are introduced and accurately investigated.

• Structural Engineering and Mechanics
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• v.59 no.6
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• pp.1019-1035
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• 2016

This paper proposes a new foundation model called "Dynamic foundation model" for the dynamic analysis of plates on foundation subjected to a moving oscillator. This model includes a linear elastic spring, shear layer, viscous damping and the special effects of mass density parameters of foundation during vibration. By using finite element method and the principle of dynamic balance, the governing equation of motion of the plate travelled by the oscillator is derived and solved by the Newmark's time integration procedure. The accuracy of the algorithm is verified by comparing the numerical results with the other numerical results in the literature. Also, the effects of mass and damping ratio of system components, stiffness of suspension system, velocity of moving oscillator, and dynamic foundation parameters on dynamic responses are investigated. A very important role of these factors will be shown in the dynamic behavior of the plate.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.451-453
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• 2013

Common root rot caused by Cochliobolus sativus is a serious disease of barley. A simple and reliable method for assessing this disease would enhance our capacity in identifying resistance sources and developing resistant barley cultivars. In searching for such a method, a conidial suspension of C. sativus was dropped onto sterilized elongated subcrown internodes and incubated in sandwich filter paper using polyethylene transparent envelopes. Initial disease symptoms were easily detected after 48h of inoculation. Highly significant correlation coefficients were found in each experiment (A, B and C) between sandwich filter paper and seedling assays, indicating that this testing procedure was reliable. The method presented facilitates a rapid pre-selection under uniform conditions which is of importance from a breeder's point of view.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2003.11a
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• pp.163-167
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• 2003

In this paper, an air-suspension type RF MEMS inductor is fabricated, and an appropriate de-embedding scheme for 3-dimenstional MEMS structure is applied and verified with inductance calculation algorithm. With the presented de-embedding method, parasitics from contanct pads and feeding lines are effectively and accurately de-embedded using open and short calibration procedure, and only spiral and posts can be characterized as a high-Q inductor structure. The validity of the de-embedding method is verified by the comparison of the measured and calculated inductances of two 1.5 and 2.5 turn square spiral inductors. The open-short de-embedded inductance error is below 5% each case in comparison with the calculated value based on H.M. Greenhouse's algorithm.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.309-314
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• 2000

Rubber is used as a primary suspension component in a vehicle or a mechanical structure. The aim of this study is to establish a procedure for measuring the material properties of rubber and to analyze the differences of material properties of virgin rubber and heat aged rubber. The effect of mechanical conditioning was shown and included in the procedures of tensile. compression. simple shear. and pure shear tests. In addition. using engine oil was recommended for compression test in order not to have barrelling. Moreover, based on the material properties of vil·gin rubber and heat aged rubber. equations of C10 and IRHD were proposed as a function of heat aging temperature and time.