• Title/Summary/Keyword: Survival Program

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Pseudomonas syringae pv. tomato DC3000 Improves Escherichia coli O157:H7 Survival in Tomato Plants

  • Namgung, Min;Lim, Yeon-Jeong;Kang, Min Kyu;Oh, Chang-Sik;Park, Duck Hwan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.12
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    • pp.1975-1981
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    • 2019
  • Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concern worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits from the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP-triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data support a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.

Over-expression of JunB inhibits mitochondrial stress and cytotoxicity in human lymphoma cells exposed to chronic oxidative stress

  • Son, Young-Ok;Heo, Jung-Sun;Kim, Tae-Geum;Jeon, Young-Mi;Kim, Jong-Ghee;Lee, Jeong-Chae
    • BMB Reports
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    • v.43 no.1
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    • pp.57-61
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    • 2010
  • Activator protein-1 can induce either cell survival or death, which is controlled by opposing effects of different Jun members. It is generally accepted that c-Jun is pro-apoptotic, but that JunD is anti-apoptotic in stress-exposed cells. Additionally, although there are reports suggesting that JunB plays a protective role, its role in stress-induced apoptosis remains unclear. Here, we investigated the role of JunB in $H_2O_2$-induced cell death using cells that over-expressed the protein or were transfected with si-JunB. Inhibition of JunB expression accelerated $H_2O_2$-mediated loss of mitochondrial membrane potential (MMP) and cytotoxicity. Conversely, over-expression of JunB protein led to significant inhibition of the MMP loss and cell death. The increase in JunB expression also attenuated nuclear relocation of apoptosis-inducing factor and mitochondrial Bcl-2 reduction that occurred following $H_2O_2$ exposure. These results suggest that JunB can signal survival against oxidant-mediated cell death by suppressing mitochondrial stress.

Evaluation of renewable energy deployment program using outcome evaluation method and its implication (Outcome evaluation method를 이용한 신.재생에너지 보급보조사업의 평가결과 및 시사점)

  • Moon, Chang-Kwon;Heo, Eun-Nyeong
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.300-300
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    • 2009
  • Outcome Evaluation은 미국의 DOE(Department Of Energy)산하 EERE(Energy Efficiency and Renewable Energy)에서 프로그램 평가를 위해 사용하는 General Program Evaluation Guide에서 언급된 5가지 평가방법 중 하나로 프로그램의 결과를 측정하여 평가하는 방법이다. 이는 프로그램을 시행한 후 일정한 기간이 지난 뒤 프로그램의 목적을 달성함으로써 얻어지는 결과를 추산하여 그 기간 동안 프로그램이 의도했던 목적을 위해 얼마나 잘 수행되었는지를 보여준다. 본 연구에서는 Outcome Evaluation을 이용하여 한국에서 진행되고 있는 신재생에너지 보급사업에 대한 평가를 하였다. 현재 한국의 신재생에너지 보급사업은 크게 보급지원제도와 기반조성사업으로 나누어져 있으나 본 연구에서는 보급지원제도에 중점을 두어 평가를 진행하였다. General Program Evaluation Guide에 따르면 Outcome Evaluation의 방법론은 engineering estimation에서 survival analysis에 이르기까지 다양한 방법론을 제시하지만 본 연구에서는 Tabulation과 Correlation을 주로 활용하였다. 먼저 평가의 대상이 되는 프로그램과 시기를 정하고 평가지표를 산정한 뒤 위와 같은 방법론을 활용해 평가를 진행했고, 그 시사점을 도출하였다.

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Survival of Erwinia amylovora on Surfaces of Materials Used in Orchards

  • Choi, Hyun Ju;Kim, Yeon Ju;Lim, Yeon-Jeong;Park, Duck Hwan
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.89-93
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    • 2019
  • Fire blight disease caused by the bacterium, Erwinia amylovora, was observed in apple and pear orchards in Korea in 2015. Since then, it has spread, sometimes over long distances to other orchards. Therefore, we examined the ability of E. amylovora to survive in soils and on the surfaces of common materials such as T-shirts, wrist bands, pruning shears, and rubber boots by both conventional PCR (cPCR) and quantitative PCR (qPCR) methods. E. amylovora was detected in all materials tested in this study and survived for sufficiently long periods to cause fire blight disease in new sites. Thus, based on the results of this study, sanitation protocols must be applied to equipment during orchard work.

A Study on Operation Concept of Naval Surface to Air Defense System with Complex Assets (함대공 방어체계 복합자산 운용개념 연구)

  • Taegu Kim;Woongjae Na;Seoyeon Yang;Yeojin Park;Donghyuk Shim;Da-Bin Ryu;Nahae Yun;In-Chul Park;Lae-Eun Kim
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.46 no.4
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    • pp.190-198
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    • 2023
  • The purpose of this study was to propose an operational concept for a ship in a fleet equipped with an interceptor missile system, a naval surface to air defense system, and to develop a simulation program that reflects it. The results of the defense activities of other ships in the fleet can be reflected by receiving information about the status of the enemy missiles. The allocation of defensive assets is based on the survival probability of the ship, not on the destruction of enemy attacks, which can be obtained as the product of the expected survival probability for each enemy missile. In addition, the concept of predicted survivability was introduced to assess the loss of future defense opportunities that would result from assigning a new command. A simulation program was also developed as a tool for realizing the proposed concept of operations, which generated cases.

An Analysis of Cancer Survival Narratives Using Computerized Text Analysis Program (컴퓨터 텍스트 분석프로그램을 적용한 암환자의 투병수기 분석)

  • Kim, Dal Sook;Park, Ah Hyun;Kang, Nam Jun
    • Journal of Korean Academy of Nursing
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    • v.44 no.3
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    • pp.328-338
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    • 2014
  • Purpose: This study was done to explore experiences of persons living through the periods of cancer diagnosis, treatment, and self-care. Methods: With permission, texts of 29 cancer survival narratives (8 men and 21 women, winners in contests sponsored by two institutes), were analyzed using Kang's Korean-Computerized-Text-Analysis-Program where the commonly used Korean-Morphological-Analyzer and the 21st-century-Sejong-Modern-Korean-Corpora representing laymen's Korean-language-use are connected. Experiences were explored based on words included in 100 highly-used-morphemes. For interpretation, we used 'categorizing words by meaning', 'comparing use-rate by periods and to the 21st-century-Sejong-Modern-Korean-Corpora', and highly-used-morphemes that appeared only in a specific period. Results: The most highly-used-word-morpheme was first-person-pronouns followed by, diagnosis treatment-related- words, mind-expression-words, cancer, persons-in-meaningful-interaction, living and eating, information-related-verbs, emotion-expression- words, with 240 to 0.8 times for layman use-rate. 'Diagnosis-process', 'cancer-thought', 'things-to-come-after-diagnosis', 'physician husband', 'result-related-information', 'meaningful-things before diagnosis-period', and 'locus-of-cause' dominated the life of the diagnosis-period. 'Treatment', 'unreliable-body', 'husband people mother physician', 'treatment-related-uncertainty', 'hard-time', and 'waiting-time represented experiences in the treatment-period. Themes of living in the self-care-period were complex and included 'living-as-a-human', 'self-managing-of-diseased-body', 'positive-emotion', and 'connecting past present future'. Conclusion: The results show that the experience of living for persons with cancer is influenced by each period's own situational-characteristics. Experiences of the diagnosis and treatment-period are negative disease-oriented while that of the self-care period is positive present-oriented.

Cancer Survival and Status of National Health Insurance in a Community (A일개 군지역 암등록자료의 국민건강보험 보장유무에 따른 암 생존율 차이)

  • Kweon, Sun-Seog;Choi, Jin-Su;Shin, Min-Ho;Kim, Hye-Yeon;Choi, Seong-Woo;Lee, Young-Hoon
    • Health Policy and Management
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    • v.19 no.2
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    • pp.127-134
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    • 2009
  • It is known that socioeconomic status(SES) of the cancer patient is associated with survival in recent studies, performed in other countries. The purpose of this study was to determine whether the association between status of national health insurance and survival is also present in a community in Jeonnam province, South Korea. The Gwangju-Jeonnam Cancer Registry, a population-based cancer registry, provided information to identify the cancer cases of study community diagnosed from 1998 to 2007. Total of 2,046 cases were identified during the period. There were significant associations between the status of national health insurance and survival for total cancer after adjusted by age, geographic accessibility to health care, and stage at diagnosis. However, this differences were not found in the analysis using only stomach and colorectal cancer cases. Despite of some limitations, this results suggest that the policy for reducing the difference according to the SES is required in national cancer management program.

Survival Factors among Medical Intensive Care Unit Patients with Carbapenemas-Producing Enterobacteriaceae (카바페넴분해효소 생성 장내세균속균종(CPE)이 획득된 내과계 중환자실 환자의 생존 영향 요인)

  • Choi, Ji Eun;Jeon, Mi Yang
    • Journal of Korean Biological Nursing Science
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    • v.22 no.4
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    • pp.249-259
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    • 2020
  • Purpose: Carbapenemase-producing Enterobacteriaceae (CPE) are associated with considerable mortality. This study was aimed to identify survival factors among medical care unit patients with CPE. Methods: We conducted a retrospective cohort; data were collected from September 2017 to June 2019 through electronic medical records. The data collected were general characteristics, disease-related characteristics, severity-related characteristics, and treatment-related characteristics. Data were analyzed based on frequency, mean, standard deviation, Chi-square test, Fisher's exact test, t-test, Pearson's correlation coefficient, and Cox proportional hazard model using SPSS/WIN 21.0 program. Results: Seventy-seven patients were included (59 survivors and 18 deceased) in the study. Univariate analysis identified factors for survival associated with acquired CPE as age (t= -1.56, p= .037), simplified acute physiology 3 (SAPS3) score of admission date (t= -2.85, p= .006), Glasgow coma scale (GCS) of CPE acquisition date (t= 2.38, p= .020), artery catheter at CPE acquisition date (χ2= 4.58, p= .032), vasoconstrictor agents use at CPE acquisition date (χ2= 6.81, p= .009), platelet at CPE acquisition date (t= 2.27, p= .025), lymphocyte at CPE acquisition date (t= 2.01, p= .048), calcium at CPE acquisition date (t= 2.68, p= .009), albumin at CPE acquisition date (t= 2.29, p= .025), and creatinine at CPE acquisition date (t= 2.24, p= .028). Multivariate Cox proportional hazard model showed that GCS at CPE acquisition date (HR= 1.14, 95% CI= 1.05-1.22), lymphocyte at CPE acquisition date (HR= 1.05, 95% CI= 1.00-1.10), and creatinine at CPE acquisition date (HR= 1.25, 95% CI= 1.04-1.49) were independent survival factors among medical intensive care unit patients with CPE. Conclusion: Based on the study results, it is necessary to develop nursing interventions that can aid in the management of patients with CPE and identify their effects.

The Effect of Topical Application of Heparin with Microneedling on Skin Flap Survival (미세바늘을 이용한 헤파린의 국소 도포가 피부 피판 생존에 미치는 영향)

  • Yang, Eunjung;Kim, Sugwon
    • Archives of Plastic Surgery
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    • v.36 no.3
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    • pp.254-261
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    • 2009
  • Purpose: The objective of this study was to evaluate the heparin effect for a viability of random - pattern dorsal flap in hairless mouse. Methods: A caudally - based random dorsal flap, measuring $1.5{\times}5cm$, was designed and heparin was applied topically after microneeding. Twenty five male hairless mice were randomly divided into control (Group1, n=5); received only microneedling (Group 2, n=5), only heparin(Group3, n=5), microneedling with saline(Group 4, n=5), and microneedling with heparin group(group5,n=5) to the flap during 7 days. The number of the capillaries were compared between the experimental groups and control group with respect to neovascularization after heparin application using imaging analysis program under hematoxylin - eosin stain. The capillary blood flow was measured by laser Doppler flowmetry. After seven days each animal was evaluated for the percentage area of the flap survival. Mann - Whitnety U test and Kruskal - Wallis statistical analysis of survival relationships was performed. Results: It can be observed increased number of the blood vessels in the experimental groups however it was not statistically significant. Blood flow of the haparin with microneedling group maintained higher than other experimental groups. Treated microneeding and heparin mice were significantly better flap viability than in controls (flap survival 67% and $54.4mm^2$ respectively; p<.01). Positive correlation was shown between flap survival rate and laser Doppler flux value only at first day after surgery. Conclusion: Heparin has a beneficial effect on capillary flow and improve peripheral circulatory disturbances in random pattern flaps.

Fitting Cure Rate Model to Breast Cancer Data of Cancer Research Center

  • Baghestani, Ahmad Reza;Zayeri, Farid;Akbari, Mohammad Esmaeil;Shojaee, Leyla;Khadembashi, Naghmeh;Shahmirzalou, Parviz
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7923-7927
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    • 2015
  • Background: The Cox PH model is one of the most significant statistical models in studying survival of patients. But, in the case of patients with long-term survival, it may not be the most appropriate. In such cases, a cure rate model seems more suitable. The purpose of this study was to determine clinical factors associated with cure rate of patients with breast cancer. Materials and Methods: In order to find factors affecting cure rate (response), a non-mixed cure rate model with negative binomial distribution for latent variable was used. Variables selected were recurrence cancer, status for HER2, estrogen receptor (ER) and progesterone receptor (PR), size of tumor, grade of cancer, stage of cancer, type of surgery, age at the diagnosis time and number of removed positive lymph nodes. All analyses were performed using PROC MCMC processes in the SAS 9.2 program. Results: The mean (SD) age of patients was equal to 48.9 (11.1) months. For these patients, 1, 5 and 10-year survival rates were 95, 79 and 50 percent respectively. All of the mentioned variables were effective in cure fraction. Kaplan-Meier curve showed cure model's use competence. Conclusions: Unlike other variables, existence of ER and PR positivity will increase probability of cure in patients. In the present study, Weibull distribution was used for the purpose of analysing survival times. Model fitness with other distributions such as log-N and log-logistic and other distributions for latent variable is recommended.