• Korean journal of applied entomology
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• v.28 no.1
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• pp.16-22
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• 1989

A series of experiments was undertaken to learn bionomics and gowt plant resistance of the seedcorn maggot, Delia platura(Meigen), under controlled(24$\pm$$2^{\circ}C$, RH70$\pm$5%, and LD 16:8h)and field conditions. The preoviposition period for the flies was 9 days. The females survived for an average of 50(3-77) and the males for 24(1-59) days. A greater proportion of flies emerged between 6:00 A.M. and 9:00 A.M., soon after the sun rise. After the over-wintering, adults started to emerge in mid-April from pupae located near the soil surface, and peaked in late April by others located deeper. The sex ratio was about 1:1 with total samples of 1,609 females and 1,641 males. Weight of pupae reared from onion was heavier than those from other diets in the laboratory, however its size was samller than that of natural flies. Considerably more eggs were laid near pea seeds than other hosts tested. Among beans, Bapmitkong with blue seed-coat and a cowpea bean strain were preferred for oviposition. 'Namcheon` cultivar was found to be susceptible to attack by the larvae in the laboratory.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.4
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• pp.587-596
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• 2006

Most of oral streptococci express the Antigen I/II (AgI/II) proteins, cell wall anchored adhesions. AgI/II protein binds to salivary agglutinin glycoprotein, a component of tooth pellicle and to ligands in other bacteria. These associations play important roles in bacterial colonization. Recently, it was reported that diverse host molecules also interact with AgI/II protein and that these interactions induce inflammatory responses from host cells. Among mutans streptococci containing -type hemolytic activity, Streptococcus mutans is a causative agent for dental caries. Compared with many other strains of S. mutans, GS-5 strain is unique in that this bacterium expresses truncated secretory AgI/II protein due to the nonsense mutation in the agI/II gene. This indicates that S. mutans GS-5 has a different clinical role and a recent report supported this idea based on the results from clinically isolated S. mutans strains. Previously, we had cloned agI/II gene from S. mutans GS-5 and generated recombinant N-terminal AgI/II protein. In this study, we further produced a hybridoma line expressing anti-AgI/II monoclonal antibodies named as 1C11A. This antibody showed high sensitivity to AgI/II protein in Western blot and ELISA. This new reagent will provide a basis for investigating the mechanisms of AgI/II-related diseases.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.6
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• pp.1405-1415
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• 1994

The soil on the behavior of the nonlinear elastic work-hardening plasticity has a variety of stress paths due to the state of soil and the test conditions. The soil with a specific volume ${\upsilon}$ in principal stress space (${\sigma}_1$, ${\sigma}_2$, ${\sigma}_3$, and ${\upsilon}$v) displays the shape of an irregular hexagonal pyramid with an end cap. With variations of ${\upsilon}$ the size of the cap is changed but its shape remains unchanged and the movement of the cap is controlled by the increase or decrease of the plastic volumetric strain. By reflecting such a property of soil various cap models have been developed by researchers. In this thesis, a constitutive model of soil with a combination of the nonlinear elastic work-hardening plastic cap and the failure surfaces of Mohr-Coulomb (M-C cap model) has been developed. According to the the results of analyses using the work-hardening plastic cap model, the normally consolidated soil under shearing has experienced the work-hardening and plastic flow (movement of the cap). But in the shearing of the overconsolidated soil the elastic behavior is shown until the stress path has reached the failure surface and the cap does not move.

• Parasites, Hosts and Diseases
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• v.55 no.3
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• pp.247-254
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• 2017

ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student's t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.

• Geophysics and Geophysical Exploration
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• v.20 no.3
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• pp.185-192
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• 2017

A sequence of earthquakes with the main shock $M_L$ 5.8 occurred on September 12 2016 in the Gyeongju area. The main shock was the largest earthquakes in the southern part of the Korean peninsula since the instrumental seismic observation began in the peninsula in 1905 and clearly demonstrated that the Yangsan fault is seismically active. The mean focal depth of the foreshock, main shock, and aftershock of the Gyeongju earthquakes estimated by the crustal model of single layer of the Korean peninsula without the Conrad discontinuity turns out to be 12.9 km, which is 2.8 km lower than that estimated based on the IASP91 reference model with the Conrad discontinuity. The distribution of the historical and instrumental earthquakes in the Gyeongju area indicates that the Yangsan fault system comprising the main Yangsan fault and its subsidiary faults is a large fracture zone. The epicenters of the Gyeongju earthquakes show that a few faults of the Yangsan fault system are involved in the release of the strain energy accumulated in the area. That the major earthquakes of Gyeongju earthquakes occurred not on the surface but below 10 km depth suggests the necessity of the study of the distribution of deep active faults of the Yangsan fault system. The magnitude of maximum earthquake of the Gyeongju area estimated based on the earthquake data of the area turns out to be 7.3. The recurrence intervals of the earthquakes over magnitudes 5.0, 6.0 and 7.0 based on the earthquake data since 1978, which is the most complete data in the peninsula, are estimated as 80, 670, and 5,900 years, respectively. The September 2016 Gyeongju earthquakes are basically intraplate earthquakes not related to the Great East Japan earthquake of March 11 2011 which is interplate earthquake.

• Tunnel and Underground Space
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• v.17 no.5
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• pp.350-359
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• 2007

Most large cut slopes of open pit mines, roadways, and railways are steeply inclined and composed with rocks that do not contain soils. However, these rock slopes suffer both weathering and fragmentation. In the case of steep slopes, falling rock and collapse of a slope may often occur due to surface erosion. Cast-in place concrete and rubble work are the most widely used earth structure-based pressure supports that act as restraints against the collapse of the rock slope. In order to overcome the shortcomings of conventional retaining walls, a segmental grid retaining wall is being used with connects precasted segments to construct the wall. In this study, laboratory model test was conducted to estimate deformation behavior of segmental grid retaining wall with configuration of rear strecher, height and inclination of the wall. In order to examine the behavior characteristics of a segmental grid retaining wall, this research analyzes the aspects of spacial displacement through relative displacement according to change in the inclination of the wall. Also, the walls behavior according to the formation and status of the rear stretcher which serves the role of transferring the load from the header and the stretcher which make up the wall, the displacement of backfill materials in the wall, and the location of the maximum load were surveyed and the characteristics of displacement in the segmental grid retaining wall were observed. The test results of the segmental grid retaining wall showed that there was a sudden increase in failure load according to the decrease in the wall's height and the size of the in was greatly decreased. Furthermore, it revealed that with identical inclination and height, the structure of the rear stitcher did not greatly affect the starting point or size of maximum horizontal displacement, but rather had a stronger effect on the inclination of the wall.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.95-99
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• 1995

We have previously established a system for plant regeneration through somatic embryogenesis and Agrobacterium-mediated transformation of Korean ginseng. In this study to produce a fungus-resistant plant, we introduced a bean chitinase gene into ginseng using the transformation system. A binary vector pChi/748 was constructed by introducing the bean basic chitinase gene into EcoRI site of pGA748 which carries the CaMV 35S promoter governing the introduced gene and neomycin phosphotransferase II(NPT-II)gene as a positive selection marker. Cotyledonary explants were cocultured with A. tumefaciens strain LBA4404 harboring the binary vertor pChi/748 for 48 h, and transferred to MS medium supplemented with l mg/L2,4-D,0.1mg/L kinetin, 100 mg/L kanamycin, and 500mg/L carbenicillin. Kanamycin-resistant calli were formed on the cut surface of cotyledonary explants after one month of culture, and subsequently they gave rise to somatic embryos. Upon transfer onto medium containing 1 mg/L each of BA and GA$_3$, most of them converted to plantlets after 5 weeks of culture. The genomic DNA of eight kanamycin-resistant regenerants was subjected to polymerase chain reaction (PCR) using two specific 21-mer oligonucleotides derived from the chitinase gene. PCR-Southern blot analysis confirmed that the chitinase gene was incorporated into six out of the eight regenerants..

• KSBB Journal
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• v.20 no.6
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• pp.458-463
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• 2005

[ $H_2$ ] from CO and water was continuously produced in a trickle bed reactor(TBR) using Citrobacter amalonaticus Y19. When the strain C. was cultivated in a stirred-tank reactor under a chemoheterotrophic and aerobic condition, the high final cell concentration of 13 g/L was obtained at 10 hr. When the culture was switched to an anaerobic condition with the continuous supply of gaseous CO, CO-dependent hydrogenase was fully induced and its hydrogen production activity approached 16 mmol/g cell/hr in 60 hr. The fully induced C. amalonaticus Y19 cells were circulated through a TBR packed with polyurethane foam, and the TBR was operated for more than 20 days for $H_2$ production. As gas retention time decreased or inlet CO partial pressure increased, $H_2$ production rate increased but the conversion from CO to $H_2$ decreased. The maximum $H_2$ production rate obtained was 16 mmol/L/hr at the gas retention time of 25 min and the CO inlet partial pressure of 0.4 atm. The high $H_2$ production rate was attributed to the high cell density in the liquid phase circulating the TBR as well as the high surface area of polyurethane foam used as packing material of the TBR.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.569-576
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• 2007

Four hundred and fifty tilapias ($6.77{\pm}0.23$ g) were assigned randomly to six groups to evaluate the feasibility of the tested antibacterial peptides (ABPs) and oligosaccharides as substitutes for antibiotics. The control group was fed with a commercial tilapia diet; other five groups were fed with the same commercial diet supplemented with konjac glucomannan (KGLM), cluster bean galactomannan (CBGAM), and three animal intestinal ABPs derived from chicken, pig and rabbit at 100 mg/kg respectively. After 21 days of feeding, growth, disease resistance, and in vivo anti-adherence were determined. Furthermore, the inhibitory effect of tested agents on adhesion of Aeromonas veronii biovar sobria (A.vbs) strain BJCP-5 to tilapia enteric epithelia in vitro was assessed by cell-ELISA system. As a result, the tested agents supplemented at 100 mg/kg show significant benefit to tilapia growth and disease resistance (p<0.05), and the benefit may be correlated with their interfering in the contact of bacteria with host mucosal surface. Although none of the tested agents did inhibit the growth of BJCP-5 in tryptic soy broth at $100{\mu}g/ml$, all of them did inhibit the adhesion of A.vbs to tilapia enteric epithelia in vivo and in vitro. In vitro mimic assays show that three ABPs at low concentrations of $25{\mu}g/ml$ and $2.5{\mu}g/ml$ have the reciprocal dose-dependent anti-adherence effect. The inhibition of ABPs may be correlated with a cation bridging and/or receptor-ligand binding, but not with hydrophobicity. The KGLM and CBGAM inhibited the adherence of BJCP-5 to tilapia enteric epithelia with dose-dependent manner in vitro, and this may be through altering bacterial hydrophobicity and interfering with receptor-ligand binding. Our results indicate that the anti-adherence of the tested ABPs and oligosaccharides may be one of the mechanisms in promoting tilapia growth and resistance to A.vbs.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.25 no.1
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• pp.71-87
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• 1995

The purpose of this study was to investigate the early irradiation changes on the ultrastructure of the capillary endothelial cell in the rat submandibular glands. For the study, 110 Sprague-Dawley strain male rats were singly irradiated to their neck regions with the doses of 2Gy, 5Gy, and 10Gy by 6MV X -irradiation, and sacrificed on the 3 hours, 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after irradiation. The authors observed the histologic and ultrastructural changes of the capillary endothelial cell using the light and electron microscopes. The results were as follows: I. In the light microscopic examination, the capillary dilation was observed on the 6 hours group and the capillary density was slightly increased on the 12 hours group after 2Gy and 5Gy irradiation. And luminal size and capillary density were decreased on the 3 days and the 7 days groups after irradiation, after then, they were recovered. But capillary density was still decreased on the 14 days group after 10Gy irradiation. 2. In the transmission electron microscopic examination, the mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed on the 3 hours group after irradiation. After then, endothelial swelling, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous pinocytotic vesicles were observed after the 1 day group after irradiation. Thickened basal lamina and numerous pinocytotic vesicles were still observed until the 7 days group after irradiation. These changes were recovered to normal on the 14 days group after 2Gy and 5Gy irradiation, but not after 10Gy irradiation. 3. In the scanning electron microscopic examination, the dilation of conduits and constriction, and meandering were observed on the 1 day group after 10Gy irradiation. These changes were observed with increased coarseness of the surface of the vascular resin casting on the 3 days group after irradiation. 4. From the above results, endothelial swelling, proliferation of cytoplasmic process, and thickening of the basal lamina appeared before the 6 hours group after irradiation. And these changes may also induce the increase of the capillary number and luminal size, after then, capillary permeability was increased via the increase of the number of pinocytotic vesicles. The changes were observed earlier and more apparent with the increase of the irradiation doses under the dose of 10Gy irradiation.