• BMB Reports
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• v.48 no.3
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• pp.184-189
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• 2015

Src homology 2-containing protein tyrosine phosphatase 2 (SHP-2) is known to protect neurons from neurodegeneration during ischemia/reperfusion injury. We recently reported that ROS-mediated oxidative stress promotes phosphorylation of endogenous SHP-2 in astrocytes and complex formation between caveolin-1 and SHP-2 in response to oxidative stress. To examine the region of SHP-2 participating in complex formation with caveolin-1, we generated three deletion mutant constructs and six point mutation constructs of SHP-2. Compared with wild-type SHP-2, binding of the N-SH2 domain deletion mutant of SHP-2 to p-caveolin-1 was reduced greatly, using flow cytometric competitive binding assays and surface plasmon resonance (SPR). Moreover, deletion of the N-SH2 domain of SHP-2 affected $H_2O_2$-mediated ERK phosphorylation and Src phosphorylation at Tyr 419 in primary astrocytes, suggesting that N-SH2 domain of SHP-2 is responsible for the binding of caveolin-1 and contributes to the regulation of Src phosphorylation and activation following ROS-induced oxidative stress in brain astrocytes.

• Textile Coloration and Finishing
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• v.20 no.2
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• pp.9-18
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• 2008

Wool has excellent properties, such as heat retention, absorbency, and elasticity, but it has a disadvantage in washability because the fabric will felt and shrink greatly. Felting causes the interlocking of the fiber surface scales with one another. Therefore, the studies on wool finishing have been focused on shrink proofing. Precedent researches on wool shrink proofing are mostly on eco-friendly method. using enzyme. The purpose of this study is to examine the effect of L-cysteine, EDTA in papain treatment of wool fabrics. The specific contents of study are as follows. Depending on pH, temperature, treatment time, enzyme concentration and L-cysteine, EDTA concentration, weight loss, tensile strength, whiteness, SEM were examined. Each papain treatment conditions depending on L-cysteine, EDTA were optimized from these properties. Papain had very low activation without activators. The optimum conditions of papain treatment were pH 7.5, temperature $75^{\circ}C$, time 30minutes(L-cysteine), 180minutes(EDTA) and papain concentration 5%(o.w.f.). In the use of papain 5%(o.w.f.), the activators optimum concentration was L-cysteine 2%(o.w.f.), EDTA 7%(o.w.f.)

• Journal of The Korean Society of Integrative Medicine
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• v.2 no.3
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• pp.49-55
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• 2014

Purpose : The purpose of this study was to investigate the effect of cross bag weight and carrying bag position type on vastus medialis oblique and vastus lateralis muscle activity ratio during step up. Method : Twenty healthy subjects participated in this study. subjects randomized performed step up with bag weight less than 15%, more than 15% of totalbody weight and without bag and carrying bag position ipsilateral and contralateral. muscle activity was measured with surface electromyography. One-way analysis of variance with repeated measures was used with a significance level of 0.05 Result : The results of this study were as follows: 1. The muscle activity ratio decreased significantly ipsilateral bag position more than 15% of body weight(p<.05). Also, the muscle activity ratio increased significantly contra lateral bag position more than 15% of body weight(p<.05). Conclusion : In conclusion, it was found that applied carrying bag position with weight on VMO,VL muscle activity ratio during step up down. Therefore carrying bag position can be used to selective activate vastus medialis oblique muscle in subjects with patellofemoral pain syndrome.

• Molecules and Cells
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• v.25 no.1
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• pp.99-104
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• 2008

Gangliosides, sialic acid-containing glycosphingolipids, are implicated in many neuronal diseases, but the precise molecular mechanisms underlying their pathological activities are poorly understood. Here we report that TLR2 participates in the initiation of ganglioside-triggered inflammatory signaling responses. Using FACS analysis and immunofluorescence microscopy, we found that gangliosides rapidly enhanced the cell surface expression of TLR2 in microglia, while reducing that of TLR4. The ganglioside-dependent increase of TLR2 expression was also observed at the messenger and protein levels. We also showed that gangliosides stimulate the interaction of TLR2 with Myd88, an adaptor for TLRs, and obtained evidence that lipid raft formation is closely associated with the ganglioside-induced activation of TLR2 and subsequent inflammatory signaling. These results collectively suggest that TLR2 contributes to the ability of gangliosides to cause inflammatory conditions in the brain.

• BMB Reports
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• v.36 no.1
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• pp.128-137
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• 2003

Matrix metalloproteinases (MMPs), zinc dependent proteolytic enzymes, cleave extracellular matrix (ECM: collagen, laminin, firbronectin, etc) as well as non-matrix substrates (growth factors, cell surface receptors, etc). The deregulation of MMPs is involved in many diseases, such as tumor metastasis, rheumatoid arthritis, and periodontal disease. Metastasis is the major cause of death among cancer patients. In this review, we will focus on the roles of MMPs in tumor metastasis. The process of metastasis involves a cascade of linked, sequential steps that involve multiple host-tumor interactions. Specifically, MMPs are involved in many steps of tumor metastasis. These include tumor invasion, migration, host immune escape, extravasation, angiogenesis, and tumor growth. Therefore, without MMPs, the tumor cell cannot perform successful metastasis. The activities of MMPs are tightly regulated at the gene transcription levels, zymogen activation by proteolysis, and inhibition of active forms by endogenous inhibitors, tissue inhibitor of metalloproteinase (TIMP), and RECK. The detailed regulations of MMPs are described in this review.

• The Journal of Korean Physical Therapy
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• v.24 no.5
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• pp.319-324
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• 2012

Purpose: This study examined the muscle activities of the erector spinae (ES) and gluteus maximus (GM) during bridging exercises with and without abdominal-hollowing. Methods: Nineteen healthy subjects with no medical history of low back pain or hip flexion contracture were enrolled in this study. The subjects performed bridging exercises with and without abdominal hollowing or with and without a one-leg lift. The muscle activities of the ES and GM were measured by surface electromyography during bridging exercises under each condition. A 2 (abdominal hollowing)X2 (one-leg lift) repeated ANOVA was used to compare the normalized muscle activities of the ES and GM. Results: The muscle activity of the ES during bridging exercise with abdominal-hollowing was significantly smaller than that without abdominal-hollowing (p=0.00). The muscle activities of the GM during bridging exercise with abdominal-hollowing were significantly greater than those without abdominal-hollowing (p=0.00). In addition, the muscle activities of the GM during bridging exercise with one-leg lifting was significantly greater than that without one-leg lifting (p=0.00). Conclusion: Bridging exercise with abdominal-hollowing appears to be more effective on activating the GM muscle than that without abdominal-hollowing minimizing the activation of the ES muscle.

• The Journal of Korean Physical Therapy
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• v.32 no.5
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• pp.302-306
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• 2020

Purpose: The purpose of this study was to investigate comparisons of vastus medialis (VMO) and vastus lateralis (VL) muscle activities according to different heights during drop landing in flatfooted adults. Methods: Fifteen subjects with a flat foot arch and 15 subjects with a normal feet arch were participated. Subjects performed a double limb drop landing task from 20, 40, and 60 cm heights. Surface electromyography was used to measure the muscle activities of the VMO and VL during drop landing. Results: There were significant differences of muscle activities in the VMO, VL, and the VMO and VL ratio between groups. The electromyography values of VMO, VL, and the VMO and VL ratio in the normal group were significantly greater than in the flat foot group, and muscle activities and the VMO and VL ratio significantly increased with landing heights in the both groups. Conclusion: Our results indicated that muscle activity patterns of VOM and VL in the flat foot group were lower at heights than in the normal group, so calf tightness was negative effects on balance and gait ability, so assessment of muscle activation patterns in the knee extensors should be considered during exercise and treatment of flat feet.

• Parasites, Hosts and Diseases
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• v.38 no.2
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• pp.103-106
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• 2000

It is well known that the cysteine proteases in excretory-secretory product (ESP) of Paragonimus westermani newly excysted metacercariae (PwNEM) are capable of degrading IgG in vitro. Recent evidence suggests that the IgG-coated surface, such as found on parasites, is one of the most effective physiologic stimuli for granulocyte activation. Therefore, this study was designed to investigate the effect of excretory-secretory product (ESP) of PwNEM on superoxide production of granulocytes stimulated with IgG The 96-well plates were coated with human IgG (0, 10, 30, $100{\;}\mu\textrm{g}/ml$) in the absence or presence of ESP. When granulocytes were incubated in the wells coated with human IgG in the presence of ESP, the level of superoxide production of granulocytes was reduced to about 90% when compared to the cells incubated in the wells coated with IgG alone. This inhibitory effect of the ESP on IgG-induced superoxide production of granulocytes was concentration-dependent. These results suggest that ESP secreted by PwNEM may be important in the control of effector functions of granulocytes stimulated with IgG in human paragonimiasis.

• Korean Journal of Applied Biomechanics
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• v.22 no.3
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• pp.341-348
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• 2012

The purpose of this study was to compare the muscle activities of the thoracic extensor(TE) and lumbar extensor(LE) during trunk lift (TL) exercise according to exercise position. Seventeen healthy subjects with no medial history of back pain were recruited for this study. Subjects performed the TL exercise in prone, quadruped and heel-sitting positions. The activities of the TE and LE were measured using surface electromyography during TL exercise in each exercise position. A one-way repeated-measures analysis of variance (ANOVA) was used to compare the normalized muscle activities of the TE and LE and the TE/LE ratio. The results showed that there was not significant effect of exercise position on the muscle activities of TE(p>.05). However, there was significant effect of exercise position on the muscle activities of LE and the TE/LE ratio(p>.05). Post hoc pair-wise comparisons with Bonferroni correction showed that both muscle activities of LE and TE/LE ratio in prone position were significantly different in those in heel-sitting and quadruped positions, during TL exercise, respectively. The TE/LE ratio was the greatest for TL exercise in heel-sitting position. Therefore, for selective activation of the TE muscle, we recommend performing the TL exercise in heel-sitting or quadruped position.

• Nuclear Engineering and Technology
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• v.31 no.2
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• pp.133-138
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• 1999

Research on the etching reaction of UO$_2$ with CF$_4$/O$_2$gas mixture plasma is carried out. The reaction rates are investigated as a function of CF$_4$/O$_2$ ratio, plasma power, and substrate temperature. It is found that there exists an optimum CF$_4$/O$_2$ ratio around 4:1 at all temperatures up to 37$0^{\circ}C$ and surface analysis using XPS X-ray Photoelectron Spectroscopy) confirms the result. Peak rate at the optimum gas composition increases with increasing temperature. Highest rate obtained in this study leaches 1050 monolayers/min. at 37$0^{\circ}C$ under r. f. power of 150 W, which is equivalent to about 0.5${\mu}{\textrm}{m}$/min. The rate also increases with increasing r. f. power, thus, higher power and higher substrate temperature will undoubtedly raise the etching reaction rate much further. This reaction seems to be an activated process, whose activation energy will be derived in the following experiments.