• Journal of the Korean earth science society
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• v.22 no.5
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• pp.415-422
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• 2001

Studies of atmospheric general circulation in the troposphere and stratosphere are very important to understand the influence of human activities on the global climate and its change. Recently, the existence of an annual cycle in the circulation has been reported by a number of studies. In this study, the residual mean meridional circulation is calculated by the TEM momentum and continuity equations for the period from December 1985 to November 1995 (10 years), and the long-term variations of the circulation and mass fluxes across the 100hPa surface are examined. The multiple regression statistical model is used to obtain quantitatively the long-term variations. This study is focused especially on mean meridional circulation in the troposphere and stratosphere associated with ENSO (El Ni${\tilde{n}}$o-Southern Oscillation) which is known as a cause of the unusual weather, global climate, and its change. The results show that the global scale troposphere-stratosphere mean meridional circulation is intensified during El Ni${\tilde{n}}$o event and QBO (quasi-biennal oscillation) easterly phase and weakened during La Ni${\tilde{n}}$o event and QBO westerly phase. The signal of Mount Pinatubo volcanic eruption in June 1991 is obtained. Due to the volcanic eruption the global scale troposphere-stratosphere mean meridional circulation is abruptly intensified.

• Journal of Navigation and Port Research
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• v.37 no.3
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• pp.257-262
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• 2013

A ship operating in rough sea may suffer from an undesirable motion which may severely degrade the performance of equipment onboard and give a person an uncomfortable feeling. Hence, roll stabilization received a considerable attention and various devices including bilge keels, stabilizing fins, gyroscopic, anti-rolling tanks, rudders and flaps have been conceived and utilized for the purpose. The Coanda effect is evident when a jet stream is applied tangential to a curved surface of a hydrofoil since then the jet increases the circulation around the foil and consequently the lift. Model tests and numerical simulation have been conducted to examine the practicality of a fixed type fin stabilizer augmented by the Coanda jet. The results show that the lift coefficient of the modified Coanda fin at the zero angle of attack identically coincides with that of the original fin at ${\alpha}=\26^{\circ}$ when Coanda jet is supplied at the rate of $C_j$ = 0.25. It is also shown that fixed type fin stabilizers for active control of the motions of ships and the other mobile units without rotation can be put to practical use if the Coanda effect is applied.

• Korean Journal of Remote Sensing
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• v.27 no.2
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• pp.181-190
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• 2011

Since the radar satellite missions such as TerraSAR-X and COSMO-SkyMed were launched in 2007, the spatial resolution of spaceborne SAR(Synthetic Aperture Radar) images reaches about 1 meter at spotlight mode. In 2011, the first Korean SAR satellite, KOMPSAT-5, will be launched, operating at X-band with the highest spatial resolution of 1 m as well. The improved spatial resolution of state-of-the-art SAR sensor suggests expanding InSAR(Interferometric SAR) analysis in urban monitoring. By the way, the shadow and layover phenomena are more prominent in urban areas due to building structure because of inherent side-looking geometry of SAR system. Up to date the most conventional algorithms do not consider the return signals at the frontage of building during InSAR phase and SAR intensity simulation. In this study the new algorithm introducing multi-scattering in layover region is proposed for phase and intensity simulation, which is utilized a precise LIDAR DSM(Digital Surface Model) in urban areas. The InSAR phases simulated by the proposed method are compared with TerraSAR-X spotlight data. As a result, both InSAR phases are well matched, even in layover areas. This study will be applied to urban monitoring using high resolution SAR data, in terms of change detection and displacement monitoring at the scale of building unit.

• Biomedical Science Letters
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• v.2 no.1
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• pp.13-20
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• 1996

A Recent discovery of surface antigens in cells has led to the success of quantitative measurement of T-cell subpopulations, and this has especially opened the way for an epoch-making development in the understanding and classification of cellular immune mechanisms. It is known that phenotypes of T-cell subpopulations exist in many forms according to the variation of species or animal experimental models. In Korea, Clonorchis sinensis still gives rise to public concern as it infects more than eighty million people and threatens the public by causing cirrhosis of the liver, or liver cancer when liver infection becomes prolonged and chronic. Up until now there has been much progress in research and improvement in the classification system of Clonorchis sinensis in the area of humoral immunity, but as for research in the area of cellular immune mechanisms, there is almost none. Knowing all these circumstances, the authors delved for the characterization of Iymphocyte subpopulations with mice as Clonorchis sinensis in the area of cellular immunity, and obtained the following results. That is, we injected Clonorchis sinensis antigens mixed in Freund's ajuvant solution intraperitoneally in mice and measured the T-cell subpopulation characterization of spleen lymphocytes with flow cytometry. The results of these measurements showed that CD2, CD5 and CD8 decreased early following injections but then in-creased again seven weeks after the injections. CD4, however, showed a slight increase shortly after the injection but then a fair increase seven weeks after the injection.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.1
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• pp.46-52
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• 2011

A study on the improvement for cycle efficiency of closed-type ocean thermal energy conversion (OTEC) was studied to obtain the basic data for the optimal design of cycle. For that, OTEC cycle with a generator, a reheater and a multi-turbine was simulated and analyzed. The basic thermodynamic model for OTEC is Rankine cycle and the surface seawater of $26^{\circ}C$ and deep seawater of $5^{\circ}C$ were used for the heat source of evaporator and condenser, respectively. Ammonia is used as the working fluid. The cycle efficiency increased when generator is added with 0.9 generator effectiveness. When the reheater and multi-turbine are applied in the basic cycle, the cycle efficiency showed 3.14% and the capacity of heat exchanger decreased for same total cycle power. For the OTEC cycle with the generator, the reheater and the multi-turbine showed the highest cycle efficiency and increased the efficiency by more than 6.5% comparing with the basic OTEC cycle.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.247-255
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• 2005

ice yield and plant growth response to nitrogen (N) fertilizer may vary within a field, probably due to spatially variable soil conditions. An experiment designed for studying the response of rice yield to different rates of N in combination with variable soil conditions was carried out at a field where spatial variation in soil properties, plant growth, and yield across the field was documented from our previous studies for two years. The field with area of 6,600 m2 was divided into six strips running east-west so that variable soil conditions could be included in each strip. Each strip was subjected to different N application level (six levels from 0 to 165kg/ha), and schematically divided into 12 grids $(10m \times10m\;for\;each\;grid)$ for sampling and measurement of plant growth and rice grain yield. Most of plant growth parameters and rice yield showed high variations even at the same N fertilizer level due to the spatially variable soil condition. However, the maximum plant growth and yield response to N fertilizer rate that was analyzed using boundary line analysis followed the Mitcherlich equation (negative exponential function), approaching a maximum value with increasing N fertilizer rate. Assuming the obtainable maximum rice yield is constrained by a limiting soil property, the following model to predict rice grain yield was obtained: $Y=10765{1-0.4704^*EXP(-0.0117^*FN)}^*MIN(I-{clay},\;I_{om},\;I_{cec},\;I_{TN},\; I_{Si})$ where FN is N fertilizer rate (kg/ha), I is index for subscripted soil properties, and MIN is an operator for selecting the minimum value. The observed and predicted yield was well fitted to 1:1 line (Y=X) with determination coefficient of 0.564. As this result was obtained in a very limited condition and did not explain the yield variability so high, this result may not be applied to practical N management. However, this approach has potential for quantifying the grain yield response to N fertilizer rate under variable soil conditions and formulating the site-specific N prescription for the management of spatial yield variability in a field if sufficient data set is acquired for boundary line analysis.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1239-1248
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• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1982-1992
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• 2019

The alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete for self-repairing. A statistical experimental design was used to develop an economical medium for its mass cultivation and sporulation. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain: the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The estimated growth and sporulation of Bacillus sp. strain AK13 in the proposed medium were 3.08 ± 0.38 × 10⁸ and 1.25 ± 0.12 × 10⁸ CFU/ml, respectively, which meant that the proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 ± 0.7 × 10⁹ CFU/g. The AK13 spore powder was mixed with cement paste, yeast extract, calcium lactate, and water. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 10⁶ CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.8
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• pp.465-471
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• 2015

Adsorption experiments for radionuclides such as $^3H$, $^{90}Sr$ and $^{99}Tc$ were conducted using fractured rock collected in unsaturated zone. The released radionuclide through artificial barrier from the near surface repository can be transported by the flow of rainfall or pore water through fractures in unsaturated zone and reach to groundwater flow. Therefore, it is important to investigate transport behavior (retardation) of radionuclides through fractured rock for the safety assessment and long-term performance of repository. Fractured rock samples were collected and characterized by X-ray microtomography (XMT) analysis, which can be used to develop a more robust unsaturated fracture transport model. When fracture-filling materials are exist, distribution coefficient of $^{90}Sr$ is higher than without fracture-filling materials. In this study, batch sorption distribution coefficient ($K_d$) of radionuclide was determined and used to increase our understanding of radionuclide retardtion through fracture-filling materials.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.305-310
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• 2003

Hydrophobic core variant of ubiquitin appeared to have partially folded structure at pH around 2. The intrinsic tryptophan fluorescence emission maximum of this ubiquitin variant at pH 2 showed slight blue shift compare to that of unfolded state, suggesting that some residual tertiary structures remain in this solvent condition. At the same solvent condition, this ubiquitin variant binds with hydrophobic dye, 8-anilinonaphthalene-1-sulfonic acid(AMS), which is known to bind to exposed hydrophobic surface. Furthermore, far-UV circular dichroic spectrum of this ubiquitin variant in the diminished pH was remarkably different from the far-UV CD spectrum of the native state or unfolded state. Based on the molar ellipticity at 220 nm, this ubiquitin variant at pH 2 appeared to have significant amount of secondary structures. All these observations suggest that this ubiquitin variant in the diminished solvent pH has loosely folded hydrophobic core with some secondary structures, which are key features of molten globule conformation. Since molten globule has long been considered as a protein folding intermediate, it is considered that this hydrophobic core variant ubiquitin will serve as a valuable model to study protein folding process.