• Journal of Animal Science and Technology
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• 제48권6호
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• pp.897-906
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• 2006

본 연구는 셀레늄강화 한우고기 생산을 위하여 셀레늄강화 버섯폐배지(Se-SMC)가 함유된 셀레늄사료(사료내 셀레늄함량: 0.9ppm)를 활용하여 급여사양기간(2개월, 3개월, 4개월)을 달리 하였을 때, 비육후기 거세한우의 조직 내 셀레늄 함량과 혈중 글루타치온 과산화효소(GSH-Px) 활성에 미치는 영향을 조사하여 최대 포화축적기간을 제시하고자 실시하였다. 본 연구에 사용된 비육후기 거세한우는 30두로 각 급여기간(2개월: 평균 체중 677kg, 3개월: 평균 체중 610kg 및 4개월: 평균 체중 524kg)에 대한 대조구 및 Se-SMC 급여구로 나누어 처리구당 5두씩 배치하여 총 6처리구로 구분하여 실시하였다. 대조구와 Se-SMC 급여구에 사용된 실험사료는 0.1과 0.9ppm의 셀레늄을 각각 함유하였다. 각 급여기간이 종료되면 도축하여 후지와 간을 채취하였으며 이를 셀레늄 함량분석에 이용하였다. 그리고 혈중 셀레늄 농도 및 글루타치온 과산화효소활성을 분석하기 위하여 도축 시에 채혈을 실시하였다. 건물섭취량은 Se-SMC 급여 및 급여기간에 의하여 유의한 차이가 나타나지 않았다. 또한 일당증체량은 대조구 및 Se-SMC 급여구간 그리고 급여기간 간에 유의한 차이가 나타나지 않았을 뿐만 아니라 총증체량 또한 각 급여기간내 대조구와 Se-SMC 급여구 간에 유의한 차이가 나타나지 않았다. Se-SMC 급여는 혈중 셀레늄농도를 유의하게 증가시켰으나(P<0.001), 급여기간에 따른 혈중셀레늄 농도에는 유의한 영향을 미치지 않았다. 아울러 혈중 GSH-Px 활성은 혈중 셀레늄농도에서 나타난 양상과 유사한 경향을 나타내었고, 급여기간에 의한 효과는 나타나지 않았다. 후지내 셀레늄 함량은 급여기간(P<0.05)이 증가함에 따라, 그리고 Se-SMC 급여(P<0.001)에 의하여 유의하게 증가하였다. 하지만, Se-SMC의 3개월 급여군과 4개월 급여군 간에는 유의한 차이가 나타나지 않았다. 간내 셀레늄 함량은 Se-SMC의 급여기간이 증가함에 따라 다소 증가하는 경향을 나타내었으나 유의한 효과는 나타나지 않았다. 하지만, 동일한 급여기간 내 Se-SMC는 대조구에 비하여 유의하게 높았다(P<0.01).이상의 결과로부터 근육내 셀레늄 축적을 위한 적정 Se-SMC 급여기간은 후지와 간내 셀레늄 함량을 고려할 때 약 2 내지 3개월의 급여가 충분할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1501-1505
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• 2008

A broiler experiment was conducted to compare the effects of duration and level of iron-methionine chelate (Fe-Met) supplementation on the iron, copper (Cu) and zinc (Zn) content of broiler meat. Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each treatment had 5 replicates of 10 birds (5 males and 5 females) each. Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control and two levels of Fe-Met (100 or 200 ppm in Fe) supplemented for either the whole period (0-5 wk) or grower period (4-5 wk). Production performance was not significantly affected by treatments. Iron content in the muscles (breast, leg and wing) and organs (liver and spleen) were significantly (p<0.05) increased as the level and duration of Fe-Met supplementation increased. The highest concentration of iron was shown in Fe-Met 200 fed for the whole period. Liver contained the highest amount of iron followed by spleen, leg muscle, wing muscle and breast muscle. Supplementation of Fe-Met 200 for the grower period resulted in higher iron concentration in liver and spleen than supplementation of Fe-Met 100 for the whole period. However, the same treatment resulted in lower iron concentration in muscles (breast, leg and wing) than the treatment of Fe-Met 100 for the whole period. In order to achieve the highest iron enrichment in the muscles, Fe-Met should be supplemented at 200 ppm in Fe for the whole period (5 wks). Fe-Met supplementation increased copper concentration in all muscles and organs except wing muscle. Zinc concentration decreased in breast and wing muscle but tended to increase in leg muscle, liver and spleen by Fe-Met 200 supplementation. Color of muscle was not significantly affected by Fe-Met treatments. However, redness of leg and breast muscle, and yellowness of leg and breast muscle tended to increase by supplementation of Fe-Met for the whole period. It was concluded that iron content of broiler meat can be effectively enriched by supplementation of 200 ppm of Fe as Fe-Met for 5 wks.

• Journal of Animal Science and Technology
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• 제59권4호
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• pp.7.1-7.13
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• 2017

Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

• Nutritional Sciences
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• 제7권1호
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• pp.35-40
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• 2004

This study was performed to evaluate the effect of iron supplementation on iron-deficiency-related indices, oxidative stress and antioxidative enzyme activity in female marathoners. Fourteen teenage female marathoners participated in the study. Subjects were divided into two groups: mild anemic and control, depending on their hemoglobin (Hb) level. The mild anemic group had significantly lower RBC count and hematocrit (Hct) and Hb levels compared to the control group. The mild anemic group (〈12.5g Hb/dI, n=7) was given iron supplements (60mg Fe/day) for four weeks during the summer training period. RBC count, Hct and Hb levels showed an increasing tendency through iron supplementation, and significant differences in these variables between the anemic and control groups disappeared in the post-period. There was no difference in plasma malondialdehyde (MDA) between the anemic and control groups. However, catalase (CAT) and glutathione peroxidase (GPx) activity were significantly higher in the anemic group. The significant difference in enzyme activity between the groups disappeared in the post-period. In addition, superoxide dismutase activity significantly decreased after iron supplementation. In conclusion, antioxidative enzyme activity was up-regulated in an anemic condition and mild iron supplementation decreased the antioxidant enzyme activity of female marathoners while improving their anemic condition.

• Asian-Australasian Journal of Animal Sciences
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• 제2권2호
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• pp.85-90
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• 1989

Forty bull calves of Sahiwal crosses were fed either urea treated or untreated rice straw with 4 levels of Gliricidia (0, 1, 2, and 4 kg fresh material). Dry matter intake (DMI) of straw and Gliricidia was measured during 2 periods. Straw intakes in period 1 and 2 were significantly different (P<0.001). Supplementation of Gliricidia depressed the DMI of straw during the second period (P<0.01), but not in the first period. Urea-ammonia treatment increased straw intake and total intake in both periods, but the increase in dry matter digestibility (DMD) of the ration was not significant (P>0.05). Liveweight gain (LWG) was increased significantly, both by urea ammonia treatment (P<0.01) and by supplementation with Gliricidia (P<0.001). Animals on treated straw gained on an average $137\;g{\cdot}d^{-1}$ more than those on untreated straw.

• Journal of Nutrition and Health
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• 제32권6호
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• pp.661-670
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• 1999

Effects of Ca supplementation on blood pressure(BP) response to dietary Na level were studied in 15 normotensive healthy college women with family history of hypertension. All subjects, randomly divided into 3 groups, ate low Na diet(1816mg/day) prepared in the laboratory during the first 2 weeks and normal Na diet(4064mg/day) of their own home for the next 8 weeks. The one group received daily 1g Ca supplement at both low and normal Na diet periods, the second group took daily 1g Ca supplement only at normal a diet period, and the last group took placebo during both periods. Average Ca and energy intakes at the basal of and during the trial ranged 450-600mg and 1735-1878kcal, respectively. Systolic/diastolic BP was decreased by 9.2-9.8/4.4-4.5 mmHg during low Na diet period and was increased again during next normal Na diet period. However simultaneous Ca supplementation during both low and normal Na diet period suppressed the elevation of systolic/diastolic BP occurred at normal Na diet period. Ca supplementation only at normal Na diet peroid did not affect the BP elevation. The increase of serum Ca and Ca/Mg ratio and the decrease of serum Na and Na/K ratio might be related to the BP lowering effects of Ca supplementation. In conclusion, Ca supplement could attenuate BP elevation induced by increasing Na intake. The BP lowering effect of Ca supplementation was not appeared at low Na intake. Further studies were needed to make it certain. Low Na intake was also confirmed as an effective diet control for lowering blood pressure.

• Asian-Australasian Journal of Animal Sciences
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• 제4권4호
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• pp.341-351
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• 1991

Sixteen Holstein heifer calves were used in an 112-day trial to study the effects of probiotic supplementation on growth performance and rumen metabolism. Calves were divided into four groups of four calves each, with two groups receiving the probiotic supplement and two groups serving as controls. Calves were limited to 1.6 kg dry matter of a corn-barley based grain mix per day. Long-stem bromegrass hay was fed as forage the first 56 days and bromegrass silage the last 56 days of the trial. Probiotic (28 g/d/calf) was fed along with the grain mix twice daily. Data were analyzed for the entire trial and also for the separate hay and silage feeding periods. Total weight gain and average daily gain were not affected (p>.05) by probiotic supplementation. Dry matter intake was lower (p<.05) and feed efficiency (kg feed/kg weight gain) was improved slightly during the hay feeding period for the probiotic-supplemented calves. Wither height gain was greater (p<.05) during the hay period and lower (p<.05) during the silage period for probiotic-supplemented calves. Heart girth gain was improved (p<.07) by probiotic supplementation, particularly during the hay feeding period (p<.05). Total rumen volatile fatty acid (VFA) concentration was higher (p<.05) with the probiotic-supplemented calves. Molar proportions of individual VFA were not affected (p>.05). Rumen ammonia-N and plasma urea-N concentration were lower (p<.05) for probiotic-supplemented calves during the hay feeding period. Total tract nutrient digestibility was not affected (p>.05). Some improvements in animal performance and changes in rumen and blood metabolites were observed when calves were supplemented with probiotic. Effects due to probiotic supplementation were most pronounced during the hay feeding period.

• Asian-Australasian Journal of Animal Sciences
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• 제30권1호
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• pp.42-50
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• 2017

Objective: Two trials were conducted in order to examine the effects of level of supplemental methionine on productive performance, dietary energetic, plasma amino acid concentration, and digestive function. Methods: Dietary treatments consisted of a steam-flaked corn-based diet containing urea as the only source of supplemental nitrogen supplemented with no supplemental amino acid (control), or control plus 1.01% lysine and 0.032%, 0.064%, 0.096%, or 0.128% methionine. In Trial 1, 150 Holstein steer calves ($127{\pm}4.9kg$) were utilized to evaluate the influence of treatments on growth-performance, dietary energetic, plasma amino acid concentration during the first 112 days of growing period. During the initial 56-d period calves received the 5 experimental diets. During the subsequent 56-d period all calves were fed the control diet. Results: During the initial 56-d period, methionine supplementation increased (linear effect, p<0.01) plasma methionine. In the presence of supplemental lysine, increases on level of methionine in diet did not affect average daily gain. However, increased gain efficiency (quadratic effect, p = 0.03) and estimated dietary net energy (NE; linear effect, p = 0.05). Estimated metabolizable methionine supply was closely associated ($R^2=0.95$) with efficiency NE utilization for maintenance and gain. During the subsequent 56-d period, when all calves received the control diet (no amino acid supplementation), plasma amino acid concentrations and growth performance was not different among groups. However, the effects of methionine supplementation during the initial 56-period carried over, so that following a 56-d withdrawal of supplementation, the overall 112-d effects on gain efficiency (quadratic effect, p = 0.05) dietary NE (linear effect, $p{\leq}0.05$) remained appreciable. In Trial 2, 5 cannulated Holstein steers were used to evaluate treatment effects on characteristics of digestion and amino acid supply to the small intestine. There were no treatment effects on flow of dietary and microbial N to the small intestine. Postruminal N digestion increased (p = 0.04) with increasing level of supplemental methionine. Methionine supplementation linearly increased (p<0.01) duodenal flow of methionine. Likewise, lysine supplementation increased an average of 4.6% (p = 0.04) duodenal flow of lysine. In steers that received non-supplemented diet, observed intestinal amino acid supply were in good agreement with expected. Conclusion: We conclude that addition of rumen-protected methionine and lysine to diets may enhance gain efficiency and dietary energetics of growing Holstein calves. Observed amino acid supply to the small intestine were in good agreement with expected, supportive of NRC (2000, Level 1).

• 운동영양학회지
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• 제13권3호
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• pp.185-192
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• 2009

The aim of this study was to examine the use of such dietary supplementation practices, opinions, taking period for dietary supplement, and among elite Judoists varying in age and gender. Subjects were recruited from Korea National Team Judoists in elite athlete training centers (Taenuing in Seoul, Korea and surrounding area), a total of 223 elite Judoists (2003y n=34, 2005y n=41, 2006y n=101, 2007y n=30, 2008y n=17). Data were collected from personal interviews with Judoists who participated at the Olympic games, Asian game and World Championship game since 2003-2008. Elite Judoists representing mean age of 2003y (men: 23.5±2.7, women: 22.6±2.4), 2005y (men: 23.4±2.6, women: 22.5±2.3), 2006y (men: 20.3±3.2, women: 21.9±2.4), 2007y (men: 25.3±2.6, women: 22.5±2.8) and 2008y (men: 23.9±3.9) completed a validated questionnaire assessing dietary supplementation practices and opinions. Older Judoists were significantly more likely to report greater dietary supplementation usage; to be advised by oneself; health food store and pharmacy retailers; to decrease taking period days per week and past 6 month. Relative to gender, significant differences were observed for the types of dietary supplementation reported; supplementation practice trend; reasons for dietary supplementation use.

• Asian-Australasian Journal of Animal Sciences
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• 제28권8호
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• pp.1133-1139
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• 2015

This study aimed to determine the effects of parenteral selenium (Se) and vitamin E supplementation on economic impact, milk yield, and some reproductive parameters in high-yield dairy cows in the dry period and in those at the beginning of lactation. At the beginning of the dry period, cows (n = 323) were randomly divided into three groups as follows: Treatment 1 (T1), Treatment 2 (T2), and Control (C). Cows in group T1 received this preparation 21 days before calving and on calving day, and cows in group T2 received it only on calving day. The cows in the control group did not receive this preparation. Supplementation with Se increased Se serum levels of cows treated at calving day (p<0.05). Differences in milk yield at all weeks and the electrical conductivity values at the 8th and 12th weeks were significant (p<0.05). Supplementation with Se and Vitamin E decreased the incidence of metritis, the number of services per conception and the service period, but had no effects on the incidence of retained fetal membrane. A partial budgeting analysis indicated that Se supplementation was economically profitable; cows in group T1 averaged 240.6$ per cow, those in group T2 averaged 224.6$ per cow. Supplementation with Se and Vitamin E has been found to increase serum Se levels, milk yield, and has positive effects on udder health by decreasing milk conductivity values and incidence of sub-clinical mastitis.