• Journal of Biomedical Engineering Research
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• v.44 no.3
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• pp.176-181
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• 2023

During cancer treatment, the patient's response to drugs appears differently at the cellular level. In this paper, an image-based cell phenotypic feature quantification and key feature selection method are presented to predict the response of patient-derived cancer cells to a specific drug. In order to analyze the viability characteristics of cancer cells, high-definition microscope images in which cell nuclei are fluorescently stained are used, and individual-level cell analysis is performed. To this end, first, image stitching is performed for analysis of the same environment in units of the well plates, and uneven brightness due to the effects of illumination is adjusted based on the histogram. In order to automatically segment only the cell nucleus region, which is the region of interest, from the improved image, a superpixel-based segmentation technique is applied using the fluorescence expression level and morphological information. After extracting 242 types of features from the image through the segmented cell region information, only the features related to cell viability are selected through the ReliefF algorithm. The proposed method can be applied to cell image-based phenotypic screening to determine a patient's response to a drug.

• Journal of Information Processing Systems
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• v.19 no.4
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• pp.417-426
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• 2023

To improve the effect of image restoration and solve the image detail loss, an image dehazing enhancement algorithm based on mean guided filtering is proposed. The superpixel calculation method is used to pre-segment the original foggy image to obtain different sub-regions. The Ncut algorithm is used to segment the original image, and it outputs the segmented image until there is no more region merging in the image. By means of the mean-guided filtering method, the minimum value is selected as the value of the current pixel point in the local small block of the dark image, and the dark primary color image is obtained, and its transmittance is calculated to obtain the image edge detection result. According to the prior law of dark channel, a classic image dehazing enhancement model is established, and the model is combined with a median filter with low computational complexity to denoise the image in real time and maintain the jump of the mutation area to achieve image dehazing enhancement. The experimental results show that the image dehazing and enhancement effect of the proposed algorithm has obvious advantages, can retain a large amount of image detail information, and the values of information entropy, peak signal-to-noise ratio, and structural similarity are high. The research innovatively combines a variety of methods to achieve image dehazing and improve the quality effect. Through segmentation, filtering, denoising and other operations, the image quality is effectively improved, which provides an important reference for the improvement of image processing technology.