• Journal of the Korean GEO-environmental Society
• /
• v.11 no.8
• /
• pp.57-63
• /
• 2010

For optimum scale design of containment area, a series of laboratory tests using column were performed in this study as followings; sedimentation test and self-weight consolidation test for dredged soil, and suspended solid concentration test for supernatant. Containment area has been designed and evaluated, based on field condition and concentration of suspended solid of effluent water. In addition, the relation between width of containment area and target concentration of suspended solid was analyzed. The results show that concentration of suspended solid decreases as the width of containment area decreases and the length of containment area increases. It was also observed that influence of change in ponding depth should be considered to predict the change in suspended solid concentration in supernatant discharged as disposal is conducted; the lower target suspended solid concentration of effluent water, the more important.

• Journal of Veterinary Clinics
• /
• v.21 no.1
• /
• pp.23-28
• /
• 2004

1,2-benzopyrone has been shown to affect on the activation and stimulation of macrophage. To examine the immunostimulating effect of 1,2-benzopyrone on the phagocytic response of canine peripheral blood mononuclear cells (PBMC) as well as polymorphonuclear cells (PMN), the phagocytic activity of phagocytes was analyzed by flow cytometry system using FITC-labelled latex. The 1,2-benzopyrone did not show any direct effect on phagocytic response of PBMC and PMN. But it showed an enhanced effect on the phagocytic response of monocyte-rich cells fractioned by cell size from dot plot profile in flowcytometric cytography of PBMC. The phagocytic activity of these cells was also enhanced by addition of culture supernatant from PBMC treated with 1,2-benzopyrone. Similarly, the phagocytic activity of PMN but not PBMC in the same procedures was enhanced by culture supernatant from PBMC treated with 1,2-benzopyrone. However, the culture supernatant from PMN treated with 1.2-benzopyrone did not show the enhancing effect on phagocytic activity for monocyte-rich cells and PMN. These results, therefore, suggested that enhanced phagocytic activity of canine peripheral blood PMN and monocytes may be mainly mediated by humoral factor(S) released from PBMC treated with 1,2-benzopyrone.

• Parasites, Hosts and Diseases
• /
• v.44 no.1 s.137
• /
• pp.35-41
• /
• 2006

Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addition, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membrane LPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchange chromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by SOS-PAGE and thin layer chromatography. The effect of mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J77 4.1 A) was studied. Both sLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amount of NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages.

• Food Science of Animal Resources
• /
• v.28 no.1
• /
• pp.105-108
• /
• 2008

The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

• Korean Journal of Veterinary Research
• /
• v.26 no.2
• /
• pp.265-276
• /
• 1986

The bovine serum factor influencing the growth of swine testicle (ST) cell and the END effect of hog cholera SN test was studied. Throughout the experimental studies. following results were obtained and summarized. 1. Bovine whole serum of 16(76.2%) and 4(19.0%) samples out of 21 have shown a positive ST cell growth and the END effect, respectively. However, all of 21(100%) and 8(38.1%) samples out of 21 serum supernatant fractions, prepared from the bovine whole serum, have shown positive ST cell growth and END effect, respectively. 2. In the SDS-polyacrylamide gel electrophoretic analysis of the bovine whole serum and the supernatant fractions, ST cell growth inhibiting factor was proved present in globulin fraction and in whole gel plate as a diffusible component. 3. The END ineffective component present in the whole serum and its supernatant fraction was proved to be BVDV neutralizing antibody. 4. The difference of osmolarity, optical density, pH, degree of precipitant formation following heat cold treatment, A/G ratio as we11 as electrophoretic pattern and NDV SN index of the samples were not correlated to the degree of 57 cell growth and to the END effectiveness.

• Korean Journal of Food Science and Technology
• /
• v.19 no.5
• /
• pp.441-445
• /
• 1987

The subcellular distribution of lipoxygenase in germinating soybean seeds (Glycine max[L.] AmSoy) was investigated by using differential centrifugation and sucrose density gradient fractionation. Most of lipoxygenase -1 and -2/3 activities was present in the supernatant fraction after differential centrifugation of homogenates prepared from three-day-old seedlings; only 1.5% of lipoxygenase activity remained in particulate fractions. The results of a sucrose density gradient fractionation (three-day-old) showed that the lipoxygenase activity coincided with acid phosphatase at the densities of 1.19, 1.23, $1.25g/cm^3$, even though most of lipoxygenase and acid phosphatase activities appeared in supernatant fractions. There was no indication that mitochondria contained any lipoxygenase activity, and it does not appear that glyoxysomes and ER contained any lipoxygenase activity either.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.7 no.1
• /
• pp.27-32
• /
• 2006

We separated semiconducting single-walled carbon nanotubes(sem-SWCNT) from the HiPco-SWCNTS by dispersion with octadecylamine(ODA). The mixture of acid-treated SWCWTS and ODA was heated at $120^{\circ}C$ for 120hours. ODA physisorbs selectively on the side-wall of sem-SWCNTS. The ODA-treated CNTs were dispersed in tetrahydrofuran(THF) via sonication. The ODA-physisorbed sem-SWCNT can be retained in the supernatant of THF, but met-SWCNT and unabsorbed sem-SWCNT were precipitated in THF. Raman spectra with 514 nm and 1074 nm were investigated. The amount of sem-SWCNT in the supernatant and precipitant was about 94 % and 50 %, respectively.

• Journal of Korean Society on Water Environment
• /
• v.22 no.3
• /
• pp.540-545
• /
• 2006

The anaerobic digester supernatant (ADS) with high $NH_4-N$ concentration often results in a $NH_4-N$ overloading to the mainstream process of municipal wastewater treatment plant (MWTP). The nitrogen removal from the ADS is therefore important in order to achieve a stable mainstream process performance as well as to prevent $NH_4-N$ overloading due to ADS. Recently because of several advantages compared to the full nitrification, many works have shown interests in controlling the build-up of $NO_2-N$ in nitritation processes. The application of nitritation could save the aeration power compared to the full nitrification processes. In addition, the denitrification of $NO_2-N$ could reduce organic carbon requirements compared to the $NO_3-N$ denitrification. The purpose of this research was to find out the characteristics of the ADS nitritation and $NO_2-N$ accumulating factors from the laboratory reactor study. As a result based on the long-term laboratory experiment, it can be concluded that the degree of nitritation was closely related with the availability of alkalinity, free ammonia (FA), solid retention time (SRT) and solid concentration in the nitritation reactor.

• The Plant Pathology Journal
• /
• v.37 no.3
• /
• pp.205-214
• /
• 2021

The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant's defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.1
• /
• pp.75-82
• /
• 2023

Lactic acid bacteria (LAB) isolated from kimchi (a traditional Korean dish typically made of fermented cabbage) can provide various health benefits, including anti-obesity, antioxidant, antiinflammatory, anticancer, and antimicrobial effects. In this study, we examined the antimicrobial and immunomodulatory effects of Lactiplantibacillus plantarum WiKim0125 cultured in de Man, Rogosa, and Sharpe (MRS) medium containing vegetable waste. Live bacterial cells were eliminated via supernatant filtration or heat treatment. The cell-free supernatant (CFS) obtained from culture broth containing kimchi cabbage waste (KCW), cabbage waste (CW), or onion waste (OW) showed significantly higher antimicrobial activity against skin pathogens (Propionibacterium acnes and Staphylococcus aureus) and foodborne pathogens (Escherichia coli and Salmonella typhimurium), with inhibition zones ranging between 4.4 and 8.5 mm, compared to that in conventional MRS medium (4.0-7.3 mm). In lipopolysaccharide-stimulated RAW264.7 cells, both supernatant and heat-inactivated Lb. plantarum WiKim0125 from culture media containing KCW and CW suppressed the production of inflammatory cytokines (72.8% and 49.6%, respectively) and nitric oxide (62.2% and 66.7%, respectively) without affecting cell viability. These results indicate that vegetable waste can potentially increase the antimicrobial and immunoregulatory potency of LAB while presenting a molecular basis for applying postbiotics to health products.