• 한국양식학회지
• /
• 제21권4호
• /
• pp.317-324
• /
• 2008

본 연구는 2005년에 생산한 넙치 선발 1세대의 초기 성장에 대한 유전모수 및 유전능력평가 결과의 재현성을 검정하기 위해 부화 후 180일령 넙치의 성장형질 측정치를 EM-REML 알고리즘(Misztal, 2001)을 이용하여 유전모수와 육종가를 추정하였으며, 추정된 육종가를 기준으로 선발된 우량 친어와 열등한 친어간의 교배에 의해 2006년에 생산된 가계를 이용하여 유전 능력평가의 재현성을 검정하였다. 넙치 선발 1세대의 부화 후 180일령 체중, 전장, 체고, 체형 지수 및 비만도의 유전력은 각각 0.564, 0.590, 0.588, 0.306, 0.332로 추정되었다. 체중 및 전장의 각 교매그룹별 최소자승평균치에 있어서 우량친어간의 교배에 의해 생산된 가계($S{\times}S$)가 각각 $145.6{\pm}1.8\;g$, $22.4{\pm}0.1\;cm$로 나타났고, 우량친어와 열등친어간의 교배에 의해 생산된 가계($S{\times}I$)가 각각 $133.2{\pm}2.5\;g$, $22.1{\pm}0.1\;cm$로 나타났으며, 열등친어간의 교배에 의해 생산된 가계($I{\times}I$)가 각각 $114.0{\pm}2.1\;g$, $21.1{\pm}0.1\;cm$로 나타났다. 체형 관련 형질인 체형지수와 비만도의 각 교배그룹별 최소자승평균치에 있어서, $S{\times}S$ 가계가 각각 $2.756{\pm}0.005$, $12.74{\pm}0.04$로 나타났고, $S{\times}I$가계가 각각 $2.817{\pm}0.008$, $12.20{\pm}0.06$으로 나타났며, $I{\times}I$가계가 각각 $2.863{\pm}0.007$, $11.90{\pm}0.05$로 나타났다. 이상의 결과에서 넙치의 성장초기인 부화 후 180일령의 유전력은 대체적으로 높게 추정되어 선발육종 대상어종으로 적합한 것으로 판단되며, 유전능력평가의 재현성도 높은 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권3호
• /
• pp.314-319
• /
• 2005

To compare two different schemes, continuous scheme (CS) and discontinuous scheme (DCS), of once-weekly ovum pick up (OPU) with ultrasound-guided follicular puncture technique, Holstein heifers were randomly divided into two groups of five. After characterization of their two normal estrous cycles, the heifers were subjected to consecutive 20 weeks of once-weekly OPU under two schemes: the CS (one week interval between continuous OPU, total 100 OPU sessions performed) and the DCS (OPU fixed to the day 3 and day 10 of each estrus). Then, the status of ovaries and artificial insemination results were observed. On oocyte yield, the total number of punctured follicles using DCS was lower than that using CS, but the mean numbers of punctured follicles and recovered oocytes per session were higher in DCS than CS group. So the total number of recovered oocytes was similar in both groups. There were also no differences in the quality of recovered oocytes, nor in the developmental ability of oocytes fertilized in vitro between groups. The heifers in the DCS group showed regular estrous cycles with stable estrous signs through the periods of before, during, and after OPU, while those in CS group showed longer estrous cycles and less estrous signs during and/or after OPU compared with before period. Furthermore, the mean number of inseminations required for obtaining pregnancy after completion of the experiments was lower in DCS than CS group. The research demonstrates that similar quantity and quality oocytes can be achieved, and the side effects on donors are lower in DCS that needs fewer OPUs than CS group, and DCS is superior to CS.

• Journal of Microbiology
• /
• 제44권3호
• /
• pp.363-368
• /
• 2006

In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.

• Animal Bioscience
• /
• 제37권4호
• /
• pp.609-621
• /
• 2024

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

• 한국농림기상학회지
• /
• 제14권4호
• /
• pp.236-245
• /
• 2012

제주지역에 도입된 편백 18가계의 초기 생장부터 현재(42년생) 까지의 연령별 수고, 흉고직경, 재적생장을 분석하였다. Yako-7, Muei-1, Sangsong-9 가계가 우수한 생장을 하였으며, Sangeun-3, Yago-6, Hyena-2 가계의 생장이 저조한 것으로 나타났다. 연령별 평균생장을 분석한 결과, 수고생장은 9년생에서 15년생 사이의 생장이 다른 연령대에 비해 우수하였으며, 흉고직경의 생장은 특별한 경향이 관찰되지 않았다. 수고, 흉고직경, 재적의 가계유전력은 각각 0.68, 0.75, 0.75로 추정되었으며, 연령간의 생장을 비교한 결과, 유노상관 관계는 없는 것으로 판명되었다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권sup3호
• /
• pp.179-183
• /
• 2016

Breast cancer is the most prevalent type of cancer among women around the world, and mortality is primarily caused by micro-metastatic disease. The complex mechanisms of breast cancer invasion and metastasis are intrinsically related to the malignant cell type so that early detection of micro-metastases can help prolongation of survival for patient. The aim of the present research work was evaluation of the expression status of mammoglobin protein as a candidate molecular marker in the negative sentinel lymph node (SLN). Fifty tumor specimens, and 50 normal adjacent breast tissue samples from the same patients were selected on the basis of having more than 10% tumor content for RNA extraction from SLNs. Tumor samples and normal adjacent breast tissue were archived in the form of frozen fresh tissue in liquid nitrogen. Real-time PCR was performed on a Bioner life express gradient thermal cycler system. Mammoglobin gene overexpression in breast cancer metastasis was investigated. Single marker results were mammaglobin 66.7% and CK19 50.0%, with 58.3% for the two in combination. Due to improved outcome with at least 3 genes (83.3%), it seems, triple marker evaluation will be most likely useful for detecting micro-metastases instead of studying separate genes.

• International Journal of Oral Biology
• /
• 제39권3호
• /
• pp.153-157
• /
• 2014

Natural biopolymers such as collagen and fibrin have been widely used in bone regenerative applications. Despite the frequent use, their comparative biological propertiesis are largely unknown. In a previous study, we found the superiority of fibrin to collagen in the adsorption of serum proteins and the proliferation and differentiation of cultured osteoblasts. In this study, we used an in vivo model to evaluate how effectively fibrin supports bone regeneration, as compared with collagen. Collagen and fibrin were placed in critical size defects made on rat calvarial bones. Compared with collagen, fibrin supported substantially more new bone tissue formation, which was confirmed by micro-CT measurement and histological analyses. The cells in the regenerative tissues of the fibrin-filled defects were immunostained strongly for Runx2, while collagen-placed defects were stained weakly. These in vivo results demonstrate that fibrin is superior to collagen in supporting bone regeneration.

• Applied Microscopy
• /
• 제28권3호
• /
• pp.299-306
• /
• 1998

T-butyl alcohol (TBA) freeze-drying method originally designed by Inoue and Osadake (1989) was adopted to dry specimens for scanning electron microscopy and the results were compared with those dried using critical point dryer (CPD). Small pieces $(1\times1\times3mm)$ of liver of Sprague-Dawley rats were cut and fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer after anesthesia, and processed for scanning electron microscopy by several modifications of TBA freeze-drying methods and by the standard preparation method using CPD. The bile canaliculi and sinusoidal endothelial surface were observed. Tissue dehydrated with TBA before TBA freeze-drying preserved the structures best comparable to those prepared with CPD. This result suggests that combination of dehydration with TBA and TBA freeze-drying is a superior method to the original TBA freeze-drying method dehydrated with ethanol.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.148.2-149
• /
• 2003

Cucumber fruit mottle mosaic tobamovirus (CFMMV) causes severe mosaic symptoms with yellow mottling on leaves and fruits, and occasionally severe wilting of cucumber plants. No genetic source of resistance against this virus has been identified. The genes coding for the coat protein or the putative 54-kDa replicase were cloned into binary vectors under control of the SVBV promoter. Agrobacterium-mediated transformation was peformed on cotyledon explants of a parthenocarpic cucumber cultivar with superior competence for transformation. R1 seedlings were evaluated for resistance to CFMMV infection by lack of symptom expression, back inoculation on an alternative host and ELISA. From a total of 14 replicase-containing R1 lines, 8 exhibited immunity, while only 3 resistant lines were found among a total of 9 CP-containing lines. Line 144 homozygous for the 54-kDa replicase was selected for further resistance analysis. Line 144 was immune to CFMMV infection by mechanical and graft inoculation, or by root infection following planting in CFMMV-contaminated soil. Additionally, line 144 showed delay of symptom appearance following infection by other cucurbit-infecting tobamoviruses. Infection of line 144 plants with various potyviruses and cucumber mosaic cucumovirus did not break the resistance to CFMMV. The mechanism of resistance of line 144 appears to be RNA-mediated, however the means is apparently different from the gene silencing phenomenon. Homozygote line 144 cucumber as rootstock demonstrated for the first time protection of a non-transformed scion from soil inoculation with a soil borne pathogen, CFMMV.

• 한국양봉학회지
• /
• 제34권4호
• /
• pp.285-293
• /
• 2019

The honey bee queen shows extreme polyandry and controlling the mating partners can only be possible either by artificial insemination or having remote isolated mating locations. Here we report on the German North Sea island of Heligoland. Because of its location 60 km from the mainland, the lack of a local population of honey bees, its size of just 1.4 ㎢ and suitable weather conditions during the months of May to July, it is considered an ideal location for controlled inseminations of high-quality virgin queen bees with drones deemed genetically superior to others. Methods how to rear virgin queen bees are described and information is provided on the numbers of queen bees, their supporting workers and drone bees that are taken to the island in the mating season. The bee most commonly involved in the Heligoland mating trials has become Apis mellifera carnica strain "Baltica". In one summer, for example, 80 virgin queens (belonging to beekeepers from nine different locations in northern Germany) each with about 600 worker bees plus two drone populations of around 2,000 drones were taken by ship to Heligoland. On their return to the mainland no later than 3.5 weeks after the mating exercise, the beekeepers could register a mating success rate of 80%. This information can help operation management of the new remote mating centre of Weedo Island, Jeonbuk in Korea, which is currently under construction.