• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.20-24
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• 2006

Plasmid DNA (pDNA) is a product of interest for many biopharmaceutical companies and research laboratories, because of increase in the number of gene therapy protocols that use nonviral vectors. This work was undertaken to study the effect of antibiotic and dissolved oxygen concentration (DOC) on the production of a ColE 1-type plasmid (pVAX1-LacZ) hosted in Escherichia coli $DH5\alpha$ and cultured in a batch fermentor with 0.751 of Terrific Broth. A decrease in the DOC from $60\%\;to\;5\%$ was shown to increase the specific pDNA concentration approximately 1.5-fold, due to the downregulation of growth. Additionally, this increase in the pDNA concentration led to a 2.2-fold increase in the purity of cell lysates obtained after cell lysis. However, the use of higher DOC led to 2.8-fold higher volumetric productivity as a consequence of a faster growth rate, reducing the fermentation time from 24 to 8 h. Interestingly, the specific pDNA concentration, and pDNA productivity and purity were always higher $(10-15\%)$ in the absence of antibiotic. Overall, the data indicate that nonselective conditions can be used without compromising yield, productivity, and purity of pDNA.

• 한국콘텐츠학회논문지
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• 제17권8호
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• pp.520-534
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• 2017

기존의 성범죄 보도는 피해자 중심으로 이루어져 피해자에게 이중 고통을 주었다. 이 연구는 성범죄 보도에서 가해자와 피해자의 위계가 어떻게 작용하는지 보도량과 네트워크 분석을 통해 밝혔다. 가해자가 유명인인 가해자 우위의 관계와 가해자와 피해자의 지위가 비슷한 수평적 관계를 비교한 결과 가해자 우위의 관계의 보도량이 더 많았다. 가해자 우위의 관계에서 언론은 가해자의 사생활을 집중적으로 보도한 반면, 가해자-피해자 수평적 관계에서는 일반인 가해자보다 여성 피해자에게 주목했다. 가해자 우위의 관계에서는 사건의 귀인을 유명인에게 했고, 가해자-피해자 수평적 관계에서는 가해자의 주장을 지지하고, 피해자에게 사건을 귀인했다. 네트워크 분석과 프레임 분석을 통해 가해자 우위의 관계에서 언론은 가해자의 인권, 사생활을 보호하지 않는다는 것을 밝혔다.

• 대한정형외과스포츠의학회지
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• 제8권2호
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• pp.121-124
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• 2009

소아에서의 슬개골 골절은 성인을 포함한 전체 슬개골 골절의 약 2% 미만에서 발생하고, 모든 소아 골절의 약 1% 정도로 매우 드물게 발생한다. 성장중인 슬개골은 성인에서의 슬개골 골절과는 달리 골연골 골절 또는 견열 골절의 형태로 흔히 발생하며, 그 형태는 주로 소매형 골절로 나타난다. 소아 슬개골의 견열 골절 또는 소매형 골절은 슬개골의 상단부 혹은 하단부에서 발생하나, 대부분 슬개골 하단부가 주를 이루며, 상단부에 생긴 경우는 드물다. 저자들은 16세 소아에서 슬개골 상단부의 소매형 골절 1례를 경험하여 이를 문헌고찰과 함께 보고하는 바이다.

• 한국운동역학회지
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• 제27권4호
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• pp.239-245
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• 2017

Objective: The bobsleigh shoes used in the start section are one of the most important equipment for improving the competition. Despite the importance of the start section, there are no shoes that are specific for bobsleigh athletes in Korea and Korean athletes have to wear sprint spike shoes and practice the start instead of wearing bobsleigh shoes. The objective of the present study was to provide data for improving the performance of Korean bobsleigh athletes by investigating the differences in their split time, plantar pressure, and forefoot bending angle based on skill levels at the start of a run under the same conditions as training conditions. Method: Six Korean bobsleigh athletes were divided into two groups, superior (n=3) and non-superior (n=3). A digital speedometer measured the split time at the start; the Pedar-X system (Novel, Germany) measured plantar pressure. Plantar pressures and split times were measured as the athletes pushed a bobsleigh and sprinted at full speed from the start line to the 10-m mark on the bobsleigh track. An ultra-high-speed camera was used to measure the forefoot bending angle during the start phase. Results: Significant between-group differences were found in split times (p<.000; superior = 2.38 s, non-superior = 2.52 s). The superior group had a larger rearfoot (p<.05) contact area, maximum rearfoot force (p<.01), and a larger change in angles 3 and 4 (p<.05). Conclusion: At the start of a bobsleigh run, proper use of the rearfoot for achieving effective driving force and increasing frictional resistance through a wider frictional force can shorten start time.

• 한국병원경영학회지
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• 제2권1호
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• pp.65-79
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• 1997

This study aims to measure in-patients' willingness to choose the same hospital, and to define the related factors. The willingness was measured by the fact whether in-patients would return to the same hospital for their future hospitalization and can be said as the essence of ascertaining patients' satisfaction. Data was collected from 236 patients hospitalized in two hospitals selected according to its superiority, one being superior in medical technique, the other being superior in facility and equipment. To enhance the comparability between the two hospitals, the department and the diagnosis were matched, and structured questionnaires were self-fill-up. The main findings are as follows. Hospitals were analyzed by their superiority : medical services, facility and equipment. In case of hospitals with superior medical services, the willingness was proportional to positive doctor-patient relationship, to satisfaction with the medical level, and to the acknowledgement of utility in cure. In case of hospitals with superior facility and equipment, the willingness was proportional to the satisfactory state of overall facility. Two types of hospitals were combined and analyzed. The willingness for choosing hospitals with superior medical services was stronger than that for choosing the other hospitals. The satisfaction with overall facility, satisfaction with medical level, acknowledgement of utility in cure, positive doctor-patient relationship, and better consultation produced higher willingness to choose the same hospital. The willingness for the option shows to what degree the hospital suffices patients' expectation. Patients' understanding views were obviously influential. The satisfaction level for medical aspect was more influential than the level for non-medical aspect.

• 한국운동역학회지
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• 제19권3호
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• pp.481-488
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• 2009

본 연구는 스쿱 동작 시 남자 우수하키 선수들의 동작 특성을 구명하고 경기력 향상을 위한 기초자료를 제공하는데 그 목적이 있다. 이를 위해 8명의 남자 대학 학키 선수들을 두 집단(우수집단 Vs. 비우수집단)으로 구분하여 스쿱 동작을 3차원 동작분석을 이용하여 운동학적 변인을 산출하고 비교분석한 결과 다음과 같은 결론을 얻었다. 첫째, 우수그룹이 비우수 그룹에 비하여 전방으로의 무게중심 이동변화가 크고 속도도 다소 빠른 것으로 나타났으나 통계적으로 유의한 차이는 없는 것으로 나타났다 (p>.05). 둘째, 스틱헤드의 전방 선속도가 우수그룹이 빠른 것으로 나타났으나 통계적으로 유의한 차이는 없는 것으로 나타났다 (p>.05). 그러나 스틱의 수직 선속도는 통계적으로 차이가 있는 것으로 나타나 (p<.05) 비우수 그룹이 우수그룹에 비하여 공을 띄우는 기술이 부족한 것으로 판단된다. 셋째, 우수그룹이 비우수 그룹에 비하여 릴리즈 시 스틱을 강하게 내전시키며 공을 투사하는 것으로 나타났으며, 두 그룹간 통계적으로 유의한 차이가 있는 것으로 나타났다 (p<.05).넷째, 우수그룹이 비우수 그룹에 비하여 릴리즈 시 상체의 강한 내회전력을 이용하여 볼을 투사시키는 것으로 나타났다 (p>.05).

• 한국작물학회지
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• 제33권4호
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• pp.386-391
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• 1988

보리의 교배육종에서 교배조합의 양부를 초기세대에 검정할 수 있는가를 Sakai의 이론에 따라 구명하기 위하여 보리의 수량형질인 추수, 입수, 수중, 개체별 입중에 대해서 5개 교배조합을 가지고 조사를 실시한 결과를 요약하면 다음과 같다. 1. 입수의 경우 3개 조합에서 그리고 수중의 경우는 1개 조합에서 상가적유전분산이 부의 방향으로 계산되었고 교배조합의 우량도를 계산할 수 없었으나 나머지 조합과 형질에서는 무난히 계산이 가능하였다. 2. 수수에 대한, 교배조합의 우량도 Y는 SB 76588$\times$SB72648 조합이 제1위였다. 또 이 조합은 F$_4$ 집단의 상위 5 % 및 10%내 식물의 평균순위에서도 2위를 하여 수수에 대해서는 이 조합이 5개 조합중 가장 좋은 조합으로 인정되었다. 3. 수당입수에 있어서는 3개 조합에서 Y값의 추정이 불가능하였으나 나머지 2조합을 가지고 보았을 때 F$_4$ 집단의 순위가 높고 Y값도 높은 밀양 006$\times$수원20003 조합이 우량조합인 것으로 보이었다. 4. 수종에서는 Y계산이 불가했던 1개 조합을 제외한 나머지 조합에서 Y에 의한 순위와 F$_4$에의 한 순위와 대체로 일치하였고 밀양006$\times$수원 20003 조합이 우수조합으로 나타났다. 5. 개체별 입중에서도 Y값에 의한 순위와 F$_4$에 의한 순위가 대체로 일치하였고 SB76588$\times$SB72648 조합이 가장 우수한 조합인 것으로 나타났다.

• 대한가정학회지
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• 제12권2호
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• pp.609-619
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• 1974

The purpose of this study is to survey Korean mother's child-rearing methods and to see what influence each layed on children's school achievement. To obtain data, a questionaire was given to 607 boys and girls, who were selected from both public and private middle schools in Seoul. The student were consisted of two distinct groups, namely superior and inferior. Five top students from each class were categorized as the superior group and five bottom ones from each class were categorized as the inferior group. The questionaire was consisted of 7 items, namely, the needing method, feeding period, weaning time, mothers general attitudes about crying, sleeping arrangement and whether mothers workers or not. Summary of this study is as follows ; 1. As far as babies were breast-fed, there was no significant between the superior group and the interior group no matter what feeding period and weaning time were. 2. When the babies were fed by milk or both by milk and breast, the most favorable weaning time for intellectual development seemed to be between 6 and 9 months after birth. 3. The mother's general attitudes to babies crying and the sleeping arrangement have definite influence on the children's school achievement. It was shown that if mothers hugged babies as soon as they cried the babies tended to be superior, but the mothers of inferior group had tendency to let alone, until the babies stopped crying or hug after finishing their urgent works. Then the superior group was allowed to sleep alone in his own bed in the mother's room. On the contrary, the inferior group was kept in their mother bed or that of other family's without being given his own bed. 4. Whether mother had a job or not did not had any influence on children's school achievement. 5. Korean mothers have tendency to hug their babies as soon as when they find them cry and feed them with breast milk. The study also shows that the mothers rearing attitudes has certain influencial effect upon the children's school achievement.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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• pp.441-443
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• 2005

According as CCFL(Cold Cathode Fluorescent lamp) of light source in Backlight unit for Note PC (Personal computer) is presently needed to low power consumption and long life time, the development focus of CCFL is going on the discharge gas, phosphor and electrode material. First of all, discharge voltage characteristic of CCFL is closely connected with electrode material For low discharge voltage, the characteristic of electrode material is needed to low work function, low sputtering ratio and superior manufacturing property. We developed new CCFL with Nb/Ni Clad electrode superior to conventional CCFL. Because Nb/Ni Clad electrode with Ni material and Nb material, the electrical characteristic is superior to other electrode materials. The electrode of Nb/Ni Clad is composed that Ni of outside material has superior manufacturing property and Nb of inside material has low work function. Nb/Ni Clad of new electrode material is made by process of Rolling mill at high pressure and heat treatment. We compared electrical characteristic of Nb/Ni clad electrode with conventional Mo electrode by measurement. Mo electrode and Nb/Ni Clad electrode of cup type with diameter 1.1 mm and length 3.0mm are used to this experiment. Material content of Mo electrode is Mo 100%. But, Nb/Ni Clad electrode is composed by content of Nb 40% and Ni 60%. The result of comparison measurement between new CCFL with Nb/Ni Clad electrode and conventional CCFL was appeared that CCFL with Nb/Ni Clad electrode had superior characteristic than conventional CCFL. As a result of experiment, we completed Note PC with low power consumption and long life time by application of new CCFL with Nb/Ni Clad electrode.

• Journal of the Korean Wood Science and Technology
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• 제48권5호
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• pp.618-630
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• 2020

Muna teakwood, especially from old stands, has been popular as raw material for timber industries in Indonesia for the past ten decades. Due to the scarcity of this wood, superior-grown seedlings of Muna teakwood have been developed and widely planted. Since there is no information on its characteristics, therefore, the aim of this research was to investigate wood characteristics of the 8-year-old superior-grown teak from Muna Island to ensure their proper utilization as raw material for wooden furniture. Wood discs and boards from basal area of three different trees were used as the samples. Macroscopic and microscopic anatomical characteristics were observed following the IAWA's list, while their physical-mechanical properties were measured following British Standard 373-57. Results showed that anatomical characteristics of this wood sample are similar to regular teakwood, but its heartwood portion is higher. Differences among trees are found in regards to wood texture, growth ring width, as well as early and latewood portion. The green moisture content was lower than that of fast-growing teak of a similar age. The wood is more stable than the old teakwood, but its specific gravity is lower. In general, mechanical properties of this wood were higher than those of the regular fast-growing teakwood, but lower than the old one. Based on its specific gravity, this superior Muna teakwood was categorized as a Strength Class of III. The wood is suitable enough for wooden furniture manufacturing.