• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.386-391
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• 2022

This study is about a technique for obtaining collagen by extracting fat by treating collagen-containing liposuction effluent in the presence of supercritical fluid. Using a supercritical solvent, a collagen extract could be obtained from animal-derived fat in a short time (about 6 hours), and about 2-3% of collagen by mass compared to the raw material could be obtained. The presence of collagen in the extract obtained by supercritical extraction was confirmed by SDS-PAGE, and it was confirmed that it was type 1 collagen having a relatively large molecular weight. In addition, the growth factors of IGF-1, bFGF, VEGF and NGF were analyzed to find out which growth factors were present in the collagen obtained by supercritical extraction, and it was found that these growth factors were contained in the extract. There was no significant difference in DNA content per mg of sample before and after supercritical treatment. Further in-depth studies are likely to be needed on decellularization technology using the supercritical process. In conclusion, the extracellular matrix obtained through the solvent extraction process using a supercritical fluid contains growth factors above a certain amount even after decellularization and removal of fat, so that it was found that not only biocompatibility is greatly increased, but also tissue regeneration can be rapidly induced.

• Nuclear Engineering and Technology
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• v.40 no.2
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• pp.107-116
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• 2008

The present paper describes the preliminary compilation, assessment and examination of the supercritical heat transfer(SCHT) database. The availability and reliability of the SCHT data are discussed. Similarities in thermodynamic supercritical properties and SCHT behaviour of water, $CO_{2}$ and R-134a have been examined and some tentative conclusions are made. Finally, the future experimental and analytical program at the University of Ottawa is described.

• Food Science and Preservation
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• v.7 no.2
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• pp.177-183
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• 2000

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83$^{\circ}$C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.

• Food Science and Industry
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• v.43 no.1
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• pp.35-52
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• 2010

• Journal of environmental and Sanitary engineering
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• v.16 no.4
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• pp.69-76
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• 2001

The catalytic oxidation of volatile organic compounds (VOCs), which were benzene and toluene, was studied in the supercritical carbon dioxide($SC-CO_2$) media. In $SC-CO_2$ media, the deep oxidation conversion of VOCs was increased with the temperature and pressure. The deep oxidation conversion in SC -$CO_2$ media is better than that in air media at same pressure condition. This can be explained by the solubility of VOCs in $SC-CO_2$. The many intermediates produced by the partial oxidation of VOCs were detected from off-line samples. The intermediates were Identified as benzene, toluene, benzaldehyde, phenol, naphthalene, 1,1`-biphenyl, benzoic acid, 3-methylphenol, 1,1'-(1,2-ethanediyl)bis- benzene, 1,1'-(1,2-ethene- diyl)bis-benzene, anthracene, and so on. The amount of intermediates was decreased as the molar radio of oxygen to carbon dioxide was decreased. When the molar ratio of oxygen to carbon dioxide was 1 : 16, the deep conversion was kept constant. Thus, the catalytic oxidation process in $SC-CO_2$ media can be combined on-line with supercritical fluid extraction of environmental matrices and supercritical regeneration of used adsorbent. Thus, the nontoxic $SC-CO_2$ media process was suggested as the new VOCs control technology.

• Analytical Science and Technology
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• v.5 no.2
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• pp.153-158
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• 1992

In this work, we developed supercritical fluid chromatographic methods for the samples which are difficult to analyze with conventional GC or HPLC. Long-chain Hydrocarbons, mink oils and soybean oils unsuitable for GC because of their low volatility or limited thermal stability were separated by SFC due to the high solvating properties of supercritical carbon dioxide fluids. In our research, a new method for the analysis of polar fatty acids and pesticides was developed. This method should be used to overcome problems with polar samples in SFC.

• Clean Technology
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• v.15 no.1
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• pp.9-15
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• 2009

Catalytic hydrodechlorination of PCBs (polychlorinated biphenyls) included in the transformer oil was carried out to detoxify PCBs and to recycle the treated oil. Catalysts such as 0.98 wt% Pt and 0.79 wt% Pd on ${\gamma}$-alumina (${\gamma}-Al_2O_3$) support, 12.8 wt% Ni on ${\gamma}-Al_2O_3$, and 57.6 wt% Ni on silica-alumina ($SiO_2-Al_2O_3$) support were used for the catalytic hydrodechlorination. Various supercritical fluids such as carbon dioxide, propane and isobutane were used as reaction media. The effects of reaction temperature, reaction time, catalysts, and supercritical fluids on the catalytic hydrodechlorination were examined in detail. The detoxification degree increased in the order of Ni > Pd > Pt. This is possibly due to higher metal loading and larger metal size of the Ni catalyst. Below $175^{\circ}C,\;scCO_2$ was found as the most effective reaction media for the catalytic hydrodechlorination of PCBs included in the transformer oil.

• Journal of Electrical Engineering and Technology
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• v.11 no.6
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• pp.1783-1786
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• 2016

The paper investigates the insulation properties of the supercritical $CO_2$ ($SCCO_2$) fluid as an insulating medium for electrical apparatuses. The insulating material is crucial for electrical apparatuses and $SF_6$ gas has been widely used for high power electrical apparatuses. There have been many research efforts to develop substituents for $SF_6$ gas because of high global warming potential. We obtained above 350 kV/mm insulation strength with 12.0 MPa $SCCO_2$. The positive and negative IEC standard pulses are applied between two 10 mm diameter spherical electrodes. The insulation strength of $SCCO_2$ is at least 2.5 times higher than that of $CO_2$ gas at 6.0MPa. The insulation strength of $SCCO_2$ fluid is about 10 times higher than that of $SF_6$ at 0.5MPa which is the ordinary operating pressure of electrical switchgears. Using the result, we expect that the time for switching and dielectric recovery could be reduced using $SCCO_2$ fluid as an insulating medium.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1857-1864
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• 2018

Phthalate plasticizers residue in food is a serious threat to public health. Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing. In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated. The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum. No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores. However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores. Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil. Our results suggested SFE is a potential approach to remove phthalate from food related products.

• Journal of Pharmaceutical Investigation
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• v.35 no.2
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• pp.89-94
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• 2005

The micron or nano-sized lysozyme as a model protein drug was prepared using solution enhanced dispersion by supercritical fluid (SEDS) process at various conditions (e.g., solvent, temperature and pressure) to investigate the feasibility of pulmonary protein drug delivery. The lysozyme particles prepared were characterized by laser diffraction particle size analyzer, scanning electron microscopy (SEM) and powder X-ray diffractometry (PXRD). The biological activity of lysozyme particles after/before SEDS process was also examined. Lysozyme was precipitated as spherical particles. The precipitated particles consisted of 100 - 200 nm particles. Particle size showed the precipitates to be agglomerates with primary particles of size $1\;-\;5 \;{\mu}m$. The biological activity varied between 38 and 98% depending on the experimental conditions. There was no significant difference between untreated lysozyme and lysozyme after SEDS process in PXRD analysis. Therefore, the SEDS process could be a novel method to prepare micron or nano-sized lysozyme particles, with minimal loss of biological activity, for the pulmonary delivery of protein drug.