• The Korean Journal of Mycology
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• v.46 no.1
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• pp.58-68
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• 2018

Sunn hemp (Crotalaria juncea) is used as a nitrogen-fixing green manure in Korea to improve soil quality, reduce soil erosion, and suppress weeds and nematodes. In 2014, wilting sunn hemp plants were observed in green manure-cultivated fields in Wanju, Korea. Leaves of the infected plants began yellowing, starting with the lower leaves, eventually leading to their death. Moreover, a number of dark perithecia were observed on the wilting stems. Six isolates were obtained from these perithecia by single spore isolation. Based on their morphological characteristics, the isolates were identified as Fusarium udum (teleomorph: Gibberella indica). Macroconidia were slightly curved with almost hooked apical cell, and microconidia were formed on false heads by monophialides. Chlamydospores were produced abundantly in the hyphae, either singly or in clusters. To confirm the identification, multilocus sequence analysis was conducted using translation elongation factor 1 alpha (TEF), calmodulin (CAL), and histone 3 (HIS3). The sequences of TEF, CAL, and HIS3 showed 94.4~96.2%, 99.7%, and 99.6~99.8% similarity to the reference sequences of F. udum in NCBI GenBank, respectively. Pathogenicity was tested on sunn hemp and two soybean cultivars using the inoculation method of soil drenching with spore suspension. The wilting symptoms were observed only in sunn hemp and one cultivar of soybean (cv. Teagwang) after 14~21 days of inoculation. This is the first report of wilt disease in sunn hemp caused by Fusarium udum in Korea.

• The Research Journal of the Costume Culture
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• v.7 no.2
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• pp.211-219
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• 1999

The purpose of this study was to trace and identify termilogies recorded im kei lim yu sa. ma(麻), kyun(絹), po(布), je(苧), and je-po(苧布) were included in this study. For the analysis of this research comparative analytical methods was employed. Some significant findings and results of this study can be summarized as follows : 1. Text : hemp is called san(麻曰三). The word san of Koryo was derived from Indian sann, san, sana, sunn, 2. Text : Kyun is called jyi or kib(絹曰及). The word jyi(及) of Koryo was from jyi or kib() of Silla. 3. Text : Hemp cloth is called bey(bei), pey(pei), bai(布曰背). Be (베) has two meanings of hemp cloth and cloth in Korean. In the text it is interpreted as hemp cloth. 4. Text : Ramie or China grass is called mar, maw, mao, mu (毛). (苧曰毛). It is considered that the word mau(mo in Korean) (毛) in text is correct and word mau(毛) meaning China grass of Koryho is derived from mah or mak that is an old Egyptian name for flax or linen. The word mau(mo) (毛) of Koryo seems to be a transliteration of mah(or mak) in Korean or a variety of it. 5. Raime cloth or China grass cloth is clalled mau shy (苧布曰毛施). Silmar words to mau shy(毛施) of Koryo were found in Chinese, Manchurian and japanese as well as in Ancient Hebrew.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.125.3-126
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• 2003

A near full-length sequence of a new tobamovirus infecting Hibiscus rosa-sinensis L. was determined. The genome consists of 58 nucleotides (nt) 5' UTR, followed by a 4.9 kb ORF which methyl transferase helicase domain (128 kDa), readthrough protein RNA dependent RNA polymerase (RdRp) 185 kDa and a 52 kDa protein. The 128 kDa protein had a maximum homology of 51.4 ％ to TMGMV and amino acids (an) were 54.3 ％ identical to TMV- vulgare strain. The 185 kDa RdRp had a maximum homology of 53.5％ to TMV-Ob and KGMMV-Y and a 59.6％ homology at the an level to CGMMV-SH. The MP gene encodes 282 aa and its theoretical molecular weight is 30.4 kDa. The nt and an sequence identities of MP ranged from 38.8％ to 43.9％ and 30.9％ to 37.9％, respectively. The CP gene encodes 163 residues and with a theoretical molecular weight of 18.2 kDa The (nt) and aa sequences of the CP were 46.9 ％ to 51.6％ and 45.3％ to 57.1％ identical to other tobamoviruses, respectively. The predicted virion origin of assembly (OAS) was located in the CP gene. Phylogenetic trees generated based on the nt and as sequences of RdRp, MP and CP genes indicated that this new virus clustered with subgroup II tobamoviruses. Although the CP ORF of this virus shared a high nt and aa sequence identity with Sunn-hemp mosaic virus (SHMV), Western analysis showed that it is serologically unrelated to SHMV. We propose the name Hibiscus virus S (HVS) for this Singapore isolate. This is the first report on a near full-length sequence of a Tobamovirus that infects hibiscus.