• The KIPS Transactions:PartB
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• v.17B no.4
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• pp.303-308
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• 2010

In this paper, we propose a medical image registration technique combining the gradient vector flow and modified conditional entropy. The registration is conducted by the use of a measure based on the entropy of conditional probabilities. To achieve the registration, we first define a modified conditional entropy (MCE) computed from the joint histograms for the area intensities of two given images. In order to combine the spatial information into a traditional registration measure, we use the gradient vector flow field. Then the MCE is computed from the gradient vector flow intensity (GVFI) combining the gradient information and their intensity values of original images. To evaluate the performance of the proposed registration method, we conduct experiments with our method as well as existing method based on the mutual information (MI) criteria. We evaluate the precision of MI- and MCE-based measurements by comparing the registration obtained from MR images and transformed CT images. The experimental results show that the proposed method is faster and more accurate than other optimization methods.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.6
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• pp.41-47
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• 2011

This paper is concerned with the DW-MCMA(Dual Weight vector Modified Constant Modulus Algorithm) adaptive equalization algorithm using the dual weight vector in order to improve the convergence characteristic and residual inter-symbol interference which are used as the performance index for an adaptive equalizer. The equalizer is used to reduce the distortion caused by the inter-symbol interference on the wireless and the wired band-limited channel that connect the transmitting system and receiving system. The CMA is widely known as the representative algorithm for equalization. In order to transmitting the mass information with a high speed through the channels, a fast convergence speed in the equalizer performance that is able to minimize overhead needed for equalization is acquired. In this paper, By the computer simulation, we confirmed that the proposed DW-MCMA has the faster convergence speed and the smaller residual inter-symbol interference than the conventional CMA and MCMA.

• Journal of Embryo Transfer
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• v.31 no.3
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• pp.161-168
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• 2016

Nucleotide metabolism in endothelium is variable between different species. Recent studies demonstrated that this variability could contribute coagulation dysfunction, even though organs of the alpha 1,3-galactosyltransferase gene knockout pig were transplanted into the primate. CD73 (ecto-5'-nucelotidase) is an enzyme at cell surface catalyzing the hydrolysis of adenosine triphosphate to adenosine, which plays role on a substance for anti-inflammatory and anti-coagulant. Thus, overexpression of CD73 in endothelial cells of the pig is considered as an approach to reduce coagulopathy. In this study, we constructed a human CD73 expression vector under control of porcine Icam2 promoter (pIcam2-hCD73), which is expressed specifically at endothelial cells, and of CMV promoter as a control (CMV-CD73). First, we transfected the CMV-CD73 vector into HEK293 cells, and then confirmed CD73 expression at cell surface by flow cytometry analysis. Next, we transfected the pIcma2-CD73 and CMV-CD73 vectors into primary porcine fibroblasts and endothelial cells. Consequence was that the pIcma2-CD73 vector was expressed only at the porcine endothelial cells, meaning that the pIcam2 promoter lead to endothelial cell-specific expression of CD73 in vitro. Finally, we nucleofected the pIcam2-hCD73 vector into passage 3 fibroblasts, and enforced hygromycin selection of 400mg/ml. We were able to obtain forty three colonies harboring pIcam2-CD73 to provide donor cells for transgenic cloned porcine production.

• The KIPS Transactions:PartB
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• v.15B no.4
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• pp.285-292
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• 2008

Color histograms have been used as feature vectors to characterize the color features of given images, but they have a limitation in efficiency by generating high-dimensional feature vectors. In this paper, we present a method to reduce the dimension of the feature vectors by applying PCA (principal components analysis) to the color histogram of a given vehicle image. With SVM (support vector machine) method, the dimension-reduced feature vectors are used to recognize the colors of vehicles. After reducing the dimension of the feature vector by a factor of 32, the successful recognition rate is reduced only 1.42% compared to the case when we use original feature vectors. Moreover, the computation time for the color recognition is reduced by a factor of 31, so we could recognize the colors efficiently.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.67.1-67.1
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• 2015

In the past two decades, diverse methods and computer codes for reconstruction of coronal magnetic fields have been developed. Some of them can reproduce a known analytic solution quite well when the magnetic field vector is fully specified by the known solution at the domain boundaries. In practical problems, however, we do not know the boundary conditions in the computational domain except the photospheric boundary, where vector magnetogram data are provided. We have developed a new, simple variational method employing vector potentials. We have tested the computational code based on this method for problems with known solutions and those with actual photospheric data. When solutions are fully given at all boundaries, the accuracy of our method is almost comparable to best performing methods in the market. When magnetic field vectors are only given at the photospheric boundary, our method excels other methods in "figures of merit" devised by Schrijver et al. (2006). Our method is expected to contribute to the real time monitoring of the sun required for future space weather prediction.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.193-199
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• 2010

We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene expression. The glycoprotein hormone hEPO is an essential for viability and growth of the erythrocytic progenitors. Retrovirus vector system used in this study has two features including tetracycline-controllable promoter and woodchuck hepatitis virus posttranscriptional regulator element (WPRE). The former is for to reduce the possibility of physiological disturbance due to constitutional and unregulated expression of hEPO gene in the transgenic chicken. The latter is for maximum expression of the foreign gene when we turn-on the gene expression. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 325 injected eggs, 28 chicks hatched after 21 days of incubation and 16 hatched chicks were found to express the hEPO gene delivered by the vector. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. The recombinant hEPO in transgenic chicken serum had N- and O-linked carbohydrate simillar to that produced from in vitro cultured cells transformed with hEPO gene.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.5 no.1
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• pp.13-19
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• 2012

In this paper, we propose the pulmonary nodule detection method based on hierarchical 3D block analysis. The proposed system consists of two main part. In the first part, we select the block which is need to analysis. In the second part, we analysis the selected blocks. We extract the shape based features of the object in the selected blocks. Support Vector Machine is applied to the extracted features to classify into nodules and non-nodules.

• Journal of Korea Multimedia Society
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• v.8 no.6
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• pp.827-835
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• 2005

In this paper, self-networking and replaceable structure in vector graphics contents are presented for wireless internet service. The wireless networks over 2G or 3G are limited in the sense of the speed and the cost. Considering these characteristics of wireless network, self-networking method and replaceable structure in downloaded contents are introduced in order to save the amount of data and provide variations for contents. During the display of contents, a certain data for the contents is downloaded from the server and it is managed appropriately for the operation of the contents. The downloaded materials are reflected to the original contents using replaceable structure. Also, the downloading and modification are independent of the play. In this implementation, the data consists of control data for control and resource data for image, sound or text. Comparing to the conventional methods which download the whole data, the amount of the transmitted data is very small since only the difference is downloaded. Also, during the play of the contents, the changes are adopted immediately. The whole functions are implemented in wireless handset and the various applications are discussed.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.34-41
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• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.525-528
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• 2002

The objective of this study is to investigate characteristics of flow by the Rod shape and the choice of the turbulent intensity enhancement section. The Rod was setup vertically to the way of a nozzle exit flow and nozzle diameter is 17mm. Rod height is 5mm and its shapes are square, triangle, and circle. Characteristics of fluid such as velocity vector distribution, kinetic energy, turbulent intensity, and etc. were visualized, observed, and considered at 3 kinds of Re No. such as 2000, 3000, and 4000. The characteristics of flow field were investigated in each case of the distance rate from the nozzle exit to impinging plate(H/B=8, 10). The temperature of water is $20^{\circ}E$ and the measurement region divided by 3 sections(I, II, III). The nozzle diameter is 17mm. As the experimental result by PIV measurement, scale of the vector profile showed a tendency to an unbalance parabola distribution as increasing of the Re No. When the impinging plates such as square, triangle, and circle shape are installed respectively in front of the flow accelerated, rod shape of the highest velocity vector is circle shape and rod shape of the highest turbulent Intensity is square shape.