• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.126-129
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• 2003

A relatively high homology between Escherichia coli prolidase and Alteromonas organophosphorous acid anhydrolase suggests that E. coli prolidase may have an activity to d egrade toxic organophosphorous compounds. To confirm this suggestion, we cloned and expressed a prolidase gene (pepQ) of E. coliBL2l. The recombinant E. coli prolidase that consisted of 443 amino acid residues exhibited activity and stereochemical selectivity against organopho sphorous compounds, although its activity was two to three orders of magnitude less than that of the other organophosphorous acid hydrolase isolated from Pseudomonas diminuta.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.510-512
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• 1997

The effects of noscapine a phthalide isoquinoline alkaloid, on dopamine biosynthesis and tyrosine hydroxylase (TH) activity in PC12 cells were investigated. Noscapine showed 74.6% inhibition on dopamine content in PC12 cells at a concentration of $20{\mu}M.$ $IC_{50}$ of noscapine was $6.8{\mu}M.$ TH activity was inhibited by the treatment of noscapine in PC12 cells (20.9% inhibition at 20 .mu.M). Therefore, the inhibition of TH activity by noscapine might be involved in at least one component of the reduction of dopamine biosynthesis in PC12 cells.

• Korean Journal of Child Studies
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• v.29 no.1
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• pp.33-48
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• 2008

This study analyzed social development and self-efficacy in use of internet in adolescents. Subjects were 510 7th, 8th, 10th, and 11th grade students in Daegu. Instruments were the modified Activities Questionnaire for Students (Heitner & Erica, 2(02), the Personality Inventory for Youth (Heitner & Erica, 2002) and the Self-Efficacy Scale (Song, 1998). Findings were that (1) male students use more synchronous (multi-game) but female students use more asynchronous (e-mail) and asocial (web-surfing) internet activity. (2) Synchronous social internet (multi-game) users were more social and more efficient than asynchronous (e-mail) and asocial (web-surfing) internet users.

• Korean Journal of Child Studies
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• v.20 no.3
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• pp.123-137
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• 1999

This study investigated the influence of audience type (mother or close friend) age, and gender on children's goals and strategies for emotional regulation in angry and in sad situations. Hypothetical vignette methodology was used with 314 children in grades 5 and 7. In angry situations, all boys and all 5th grade children regulated anger more with instrumental motives while 7th grade girls showed more prosocial motives. Children showed more prosocial and rule oriented motives with peers and relational motives with mothers. In angry situations, children used aggression regulation strategies more toward peers and activity regulation strategies more toward mothers. Children's age and sex explained sadness regulation motives better than audience type with peers, but children used more activity regulation strategies with mothers in sad situations. When sad, fifth graders used more verbal and facial expression strategies than 7th graders while boys used more activity regulation strategies than girls.

• Textile Coloration and Finishing
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• v.23 no.3
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• pp.187-194
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• 2011

This study used Jeju scoria to dye cotton fabric and measured its dyeability, colorfastness, antibacterial activity, deodorization efficiency, ultraviolet protection, and far-infrared emission. The cotton fabric was colored to yellowish red and optimal dyeing can be achieved at a temperature of $80^{\circ}C$ for a dyeing time of 120 minutes with a colorant concentration of 25%(o.w.b). The colorfastness to light, rubbing, perspiration, and washing was 8, 5, 5, and 4~5 ratings respectively, where the wash colorfastness remained after 15 wash cycles. The cotton fabric dyed with Jeju scoria demonstrated excellent antimicrobial activity to Staphylococcus aureus and high deodorization efficiency. Ultraviolet protection factor was as high as 50+. The Jeju scoria can be used as a new colorant for the natural dyeing of silk.

• BMB Reports
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• v.31 no.4
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• pp.339-344
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• 1998

The plant flavonoids, including apigenin which is found in especially high concentrations in edible plants, are reported to protect against chronic diseases including several types of cancer. We observed that apigenin inhibited not only the growth of human melanoma cell line SK-MEL 28 but also the telomerase activity. We also noted the telomerase activity inhibition by genistein, a tyrosine kinase inhibitor found in soybean, which suggests that the telomerase activity of SK-MEL 28 cells may be regulated by the protein phosphorylation. Furthermore, we observed that apigenin induced SK-MEL 28 cell apoptosis with p53 upregulation. Taken together, our results indicate that apigenin plays a role as an antimelanoma component in edible plants.

• Microbiology and Biotechnology Letters
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• v.13 no.1
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• pp.65-70
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• 1985

The production media, partial purification and the enzymatic characteristics of laccase from Pleurotus ostreatus were investigated. Among various media tried the double strength onion media showed much higher production of laccase compared to glucose and/or CMC added media. The laccase from Pleurotus ostreatus has the optimum pH of 5.94 for the activity and appears to be stable at relatively broad pH spectrum (4.7-8.7). The experiments on the temperature stability shows that above 90% activity could be preserved after holding at $30^{\circ}C$ for 40 min., 60% activity was remained after 40 min. at $50^{\circ}C$. The Km value of the laccase from Pleurotus ostreatus was estimated to be 3.209mM and the molecular weight of laccase was estimated to be 55,000 by SDS-Polyacrylamide gel electrophoresis.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.526-529
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• 2002

Lysis of erythrocytes isolated from human, rabbit, and mouse blood samples was investigated with the culture supernatant of Xenorhabdus nematophilus in a primary form. Prior to use, the culture supernatant of the bacteria was concentrated and the concentrate was dialyzed against Tris-HCl buffer (10 mM, pH 8.1) by ultrafiltration using PM-5 membrane with a molecular weight cut-off of 5,000. At $30^{\circ}C$, the supernatant exhibited no lytic activity towards three types of erythrocytes. However, at $4^{\circ}C$, the supernatant showed selective lytic activity towards rabbit erythrocytes within 90 min. yet did not lyze human or mouse erythrocytes. Microscopic examination clearly revealed that most of the rabbit erythrocytes had been fumed into ghost forms.

• Journal of Applied Biological Chemistry
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• v.49 no.4
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• pp.131-134
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• 2006

The in vitro anti-fungal activity of hydroxylated fatty acids obtained from microbial conversion by Psuedomonas aeruginosa PR3 using ricinoleic acid(RA), eicosadienoic acid(EDA) and conjugated linoleic acid(CLA) as substrates, was investigated. Bioconverted hydroxylated fatty acids showed different anti-fungal activities potentials against the range of phytopathogenic fungi such as Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Sclerotonia sclerotiorum, Colletotricum capsici, Fusarium solani and Phytophthora capsici. RA and EDA showed up to 50% fungal mycelial inhibition at the concentration of $5{\mu}l\;ml^{-1}$. RA, EDA and CLA also exhibited anti-fungal activities with minimum inhibitory concentration(MIC), ranging from 500 to $1000{\mu}g\;ml^{-1}$. Screening was also carried out using varied concentrations of bioconverted RA and EDA for determining the anti-fungal effect on the spore germination of different fungi. Bioconverted RA and EDA showed a considerable degree of spore germination inhibition.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.271.3-272
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• 2002

This study shows that sphingosine-1-phosphate (SPP) significantly inhibits melanin synthesis in a concentration-dependent manner, and that the activity of tyrosinase was also reduced in SPP-treated cells. In contrast. a specific extracellular signal-regulated protein kinase (ERK) pathway inhibitor, PD98059 increased tyrosinase activity and melanin production, and PD98059 restored the reduced tyrosinase activity and pigmentation induced by SPP. (omitted)