• International Journal of Oral Biology
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• 제30권3호
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• pp.77-84
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• 2005

EGCG[(-)-epigallocatechin gallate], is a major component of green tea has been considered as a major antioxidant constituent. It has been considered as potential chemopreventive and chemotherapeutic agents. However, very little is known about the cellular actions by which EGCG mediates its therapeutic effects. Various aspects of antioxidant activity of EGCG were evaluated in this study. EGCG itself did not show significant cytotoxicity. Significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was observed in all ranges of concentration ($0.8-100{\mu}g/ml$) used in this study. Protective effect of EGCG against hydrogen peroxide induced cell death was observed. Relatively high lipid peroxidation inhibitory activity were detected ($IC_{50}$ was about $20{\mu}g/ml$). EGCG also dose-dependently enhanced the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in V79-4 cells. In concentrations of $100{\mu}g/ml$ of EGCG, activities of SOD, CAT and GPX were measured as 36.9 U/mg of protein, 22.9 U/mg of protein and 17.8 U/mg of protein, respectively. When these values were compared with those of the control groups (24.9 U/mg of protein, 14.9 U/mg of protein and 11.7 U/mg of protein), the relative increases were calculated as 48, 54 and 52%, respectively. Taken together, our findings suggest that EGCG can act as an antioxidant by scavenging radicals and enhancing antioxidant enzyme activities.

• Archives of Pharmacal Research
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• 제26권6호
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• pp.463-465
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• 2003

Four protostane-type triterpenes, alisol B 23-acetate (1a), alisol C 23-acetate (2a), alisol B(3a), and alisol A 24-acetate (4a), were isolated from the rhizome of Alismatis plantago-aquatica L. var. orientale Samuelson (Alismataceae) and eleven protostane derivatives (compounds 1-11) were obtained by selective modification from alisol B 23-acetate (1a). These compounds were investigated for their anti-complement activity against the classical pathway of the complement system. Alisol B (3a) and alisol A 24-acetate (4a) exhibited anti-complement activity with $IC_{50} values of 150 and 130 \mu$ M. Among the synthetic derivatives, the tetrahydroxylated protostane triterpene (9) showed moderate inhibitory activity with $IC_{50} value of 97.1 \mu$ M. Introduction of an aldehyde group at C-23 (10; $IC_{50} value, 47.7 \mu$ M) showed the most potent inhibitory effect on the complement system in vitro.

• Natural Product Sciences
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• 제14권4호
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• pp.249-253
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• 2008

Two oleanane-type triterpenes (1, 2) and their glycosides (4-6), and one ursane-type triterpene (3) have been isolated from a methanolic extract of Patrinia saniculaefolia Hemsley (Valerianaceae) through repeated silica gel and reversed-phase C-18 column chromatography. Their chemical structures were determined as oleanolic acid (1), oleanonic acid (2), 23-hydroxyursolic acid (3), 3-O-${\alpha}$-L-arabinopyranosyl-oleanolic acid (4), 3-O-${\beta}$-D-glucopyranosyl-oleanolic acid (5), and oleanolic acid 3-O-[${\alpha}$-D-xylopyranosyl-($1{\rightarrow}3$)-${\beta}$-D-glucuronopyranoside-6-O-butyl-ester] (6) on the basis of their MS, $^1H$-, and $^{13}C$-NMR spectral data. All compounds were isolated from the whole plant of the P. saniculaefolia for the first time. These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Among them, compounds 1 - 3 exhibited anti-complement activity with $IC_{50}$ values of 470.1, 212.2, and 121.0 ${\mu}M$, respectively, whereas compounds 4 - 6 were inactive. These results suggest that the carbonyl or hydroxy group at C-3 in the oleananeand/or ursane-triterpenes are important for the anti-complement activity against the classical pathway.

• 한국육종학회지
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• 제40권4호
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• pp.422-429
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• 2008

본 실험에서 Lilium longiflorum과 11품종에 대한 biochemical, enzymology 등의 분석방법을 적용하여, 화색생합성조절유전자 chalcone synthase(CHS), flavanone 3-hydroxylase(FHT)의 활성을 분석한 결과는 다음과 같다. 1. Flavonoid의 기본골격형성을 위한 첫 효소인 CHS가 malonyl-CoA와 p-coumaroyl-CoA 기질을 촉매하여 naringenin(NAR) 산물을 생성하였고, caffeoyl-CoA와 p-coumaroyl-CoA 기질에는 eridictiol(ERI)를 생성하지 않아, 백합의 CHS는 기질특이성을 나타냈다. 2. L. longiflorum에서 FHT 효소는 NAR를 기질로써 사용하여, dihydrokaempferol(DHK) 산물을 생성하였고, 그 외 11백합품종에서도 FHT 효소의 뚜렷한 활성이 확인되었다.

• 대한방사선기술학회지:방사선기술과학
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• 제41권6호
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• pp.637-641
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• 2018

To compare of body composition and backmusclestrength between physical activity group (PAG) and non-physical activity group (Non-PAG). From Jun 14 to July 13 2018, 66 students were divided into PAG (male 20, female 13) and Non-PAG (male 18, female 15) from questionnaires for general characteristics and activity. All participants were measured body composition (Inbody 720, Biospace, Korea) and backmusclestrength (T.K.K. 5402, TAKEI, Japan) after obtaining written informed consent. Statistical analysis was performed by independent t-test and cross-sectional test using SPSS ver. 19.0 (USA). Sex and body mass index were not significant difference between two groups. Exercise was significant difference between PAG and Non-PAG(100% vs. 45.5%, p<0.001), especially running and push-up, sit-up(p<0.001). Body composition was no significant difference between PAG and Non-PAG (p>0.05). Backmusclestrength was significantly higher in PAG than that in N on-PAG (p<0.01). It is suggested that exercise affects to Backmusclestrength.

• 한국보건간호학회지
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• 제33권1호
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• pp.20-32
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• 2019

Objectives: This study identified biopsychosocial factors associated with metabolic syndrome among Korean men and women aged over 40 years. Methods: Secondary data from the 2010-2016 Korean National Health and Nutrition Examination Survey were used for this study. Based on the biopsychosocial model, psychosocial factors (stress, depression, smoking, binge alcohol consumption, physical activity) were assessed with control of biomedical (age, body mass index, family history of hypertension, dyslipidemia, type 2 diabetes mellitus, and cardiovascular disease) and biosocial factors (educational level and economic status). Data from 8,624 men and 7,321 women were analyzed by logistic regression analysis using a complex sample procedure. Results: Among men, current or past smoking habits (Adjusted Odds Ratio [AOR]: 1.349; 95% Confidence Interval [CI]: 1.155-1.575, p<.001) and binge alcohol consumption (AOR: 1.570, CI: 1.389-1.774, p<.001) were associated with metabolic syndrome. In addition, moderate (AOR: 1.205, CI: 1.038-1.400, p=.014) and low levels of physical activity (AOR: 1.296, CI: 1.109-1.514, p=.001) were associated with metabolic syndrome. Among women, low level of physical activity (AOR: 1.276, CI: 1.017-1.602, p=.036) was associated with metabolic syndrome. Conclusion: Gender specific interventions such as encouraging physical activity and prevention of smoking and excessive alcohol drinking are important to prevention of metabolic syndrome.

• Physical Therapy Rehabilitation Science
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• 제13권1호
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• pp.134-142
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• 2024

Objective: This study aimed to compare the subjective health status, physical activity index, and perceived stress rate of obese children with those of healthy children through propensity matching analysis and to use the results to help manage obese children. Design: Descriptive correlation study. Methods: This study conducted a secondary analysis using raw data from the National Health and Nutrition Examination Survey. Propensity matching (1:2) was conducted between obese and healthy adolescents. Results: The number of days of physical activity was 1.22 days for the obese adolescent group and 1.01 days for the non-obese adolescent group, which was higher in the obese group (p=0.003). The subjective health status was 3.43 and 3.81 in the obese and non-obese adolescent groups, respectively, showing a higher difference in the normal group (p<0.001). No statistical difference was observed in the perceived stress rate (p=0.871) or strength exercises (p=0.190) between the two groups. Conclusion: This study suggests the need for development measures to effectively improve youth physical activity, muscle-strengthening programs, stress management, and subjective health status in obese and normal groups.

• 한국식품영양학회지
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• 제25권4호
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• pp.747-754
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• 2012

The objective of this study was to investigate free amino acid composition, antioxidant activity, alcohol dehydrogenase activity and the sensory quality attributes for the development of functional soy sauce using Hutgae (Hovenia dulcis Thunb) fruit, which is well-known for improving liver function and alleviating various negative physiological effects following heavy consumption of alcoholic beverages. Soy sauces adding six types of extract from Hutgae fruit (HF) were prepared (SSH1: HF 20%, SSH2: HF 10%, SSH3: HF 20%/40 days NaCl extract, SSH4: HF 20%/20 days NaCl extract, SSH5: HF 20% water bath extract, SSH6: freeze-drying powder from HF 20% aqueous extract), compared with soy sauce using the conventional method. These soy sauces were used for determining alcohol dehydrogenase activity by NADH absorbance, the antioxidant effect by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and sensory evaluation by sensory scaling. Total free amino acid contents for most samples were in the range of 327.3 to 375.5 mg%, and then, aspartic acid and glutamic acid content of SSH1 and SSH5 were higher than that of others. DPPH radical scavenging activity was shown to be the highest in SSH4, also SSH1, SSH5 and SSF6 were shown to be higher than the control group. Alcohol dehydrogenase activity was shown to be the highest in SSH5. In sensory evaluation, the highest intensity of roast smell was observed in SSH4 while sweet taste was shown to be the highest in SSH5, and SSH3 and SSH5 revealed higher overall acceptability. From these results, Hutgae fruit soy sauces demonstrated antioxidant activity and alcohol dehydrogenase activity. In conclusion, soy sauces containing the water bath extract of Hutgae fruit may be used as a functional seasoning.

• Perspectives in Nursing Science
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• 제15권1호
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• pp.18-28
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• 2018

Purpose: This study was designed to explore the levels of physical activity and fatigue among nurses and to identify the relationship between these variables. Methods: Participants were 89 nurses working at an intensive care unit and an operating room in a children's hospital. Data were collected using self-reported questionnaires including the International Physical Activity Questionnaire and Brief Fatigue Inventory. Results: Most of the nurses' physical activity was work related, and there was a significant difference according to the working unit. Nurses with higher work stress and lower job satisfaction showed higher levels of fatigue than their counterparts did. Work-related physical activity and the interference of fatigue with relationships were related positively, whereas transport and leisure time physical activity were negatively related to usual fatigue and the interference of fatigue with life enjoyment. Conclusion: The levels of physical activity and fatigue of nurses were higher than those observed among other populations. The appropriate level of physical activity for these nurses needs to be investigated carefully. Also, in the intervention for nurses' fatigue, their level of work-related and leisure-time physical activity should be considered separately, and their work environment and psychological satisfaction level should be included.

• 한국식품저장유통학회지
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• 제15권2호
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• pp.269-273
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• 2008

매실의 개체당 평균 수분 함량은 $88.34{\sim}90.04%$ 범위로서 수확시기에 따른 차이가 없었으며 조단백질과 조지방은 수확시기별로 수확시기가 늦어질수록 함량이 다소 감소하였으나 조섬유는 수확시기가 늦어짐에 따라 함량이 증가하였다. 또한 polyphenol 함량은 매실이 성숙함에 따라 점차 증가하는 경향을 보였다. methanol, water, hexane, ethylacetate 및 ether 추출물의 항균활성 검색 결과 methanol에서 가장 크게 나타났으며 수확시기가 늦어질수록 항균활성은 증가하는 경향을 나타내었다. 항균활성을 가진 메탄을 추출물의 활성은 $100^{\circ}C$에서 30분 가열한 조건에서도 안정함을 확인하였고, pH 변화에 따른 안정성은 큰 차이를 보이지 않았다. 용매에 따른 전자공여능은 methanol > ethylacetate > water > ether > hexane 순으로 높게 나타났으며 수확시기에 따른 차이는 크지 않았다.