• Korean Journal of Ecology and Environment
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• v.49 no.4
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• pp.385-392
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• 2016

Geographical isolation may lead to the populations having different ecological characteristics. Geographic isolation have been known to cause echolocation call differences in bats. In order to look at geographic differences of echolocation calls of Great Horseshoe Bats (Rhinolophus ferrumequinum), populations of inland and Jeju island habitats were examined in Korea. The study areas were some abandoned mines in the inland and caves of Jeju island, which were known as rest sites of Great Horseshoe Bats during the active season. Recording was done in two ways: Hand-held, Free-flying. Recording pulse was analyzed into five parameters: Maximum Frequency (FMAX), Minimum Frequency (FMIN), Peak Frequency (PF), Duration (D), Inter pulse Interval (IPI). Interestingly, The present study shows that the difference in echolocation between the inland and island populations. The PF of bats inhabited inland was 69 kHz. but, the PF of bats inhabited island (Jeju) was 71 kHz. There was a difference between regions. Discriminant analyses also showed clear difference between the inland and the island populations. Especially, PF of Korea population is lower than that of Europe (82 kHz) and higher than that of Japan (65 kHz).

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.100-105
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• 2017

Under natural conditions, black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. Oviposition by black soldier flies is dependent on light intensity and wavelength. Therefore, continuous mass rearing of this fly requires determination of optimal artificial conditions of illumination. In this study, we compared the number of eggs laid under an artificial light source (750 watt HPL lamp) versus nature sunlight. Our results showed that compared to oviposition under natural sunlight, the use of one or two lamps for 7 hours, resulted in only 43 and 76%, of the total number of eggs laid under natural sunlight, respectively. We also investigated the hatchability of oviposited eggs under artificial illumination and under natural sunlight. The hatching rate under the former was much significantly lower than under the latter. Further detailed research is required to develop methods for successful mass rearing of black soldier fly throughout the year by means of an indoor system.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.51.3-51.3
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• 2019

Solar magnetograms are important for studying solar activity and predicting space weather disturbances1. Farside magnetograms can be constructed from local helioseismology without any farside data2-4, but their quality is lower than that of typical frontside magnetograms. Here we generate farside solar magnetograms from STEREO/Extreme UltraViolet Imager (EUVI) $304-{\AA}$ images using a deep learning model based on conditional generative adversarial networks (cGANs). We train the model using pairs of Solar Dynamics Observatory (SDO)/Atmospheric Imaging Assembly (AIA) $304-{\AA}$ images and SDO/Helioseismic and Magnetic Imager (HMI) magnetograms taken from 2011 to 2017 except for September and October each year. We evaluate the model by comparing pairs of SDO/HMI magnetograms and cGAN-generated magnetograms in September and October. Our method successfully generates frontside solar magnetograms from SDO/AIA $304-{\AA}$ images and these are similar to those of the SDO/HMI, with Hale-patterned active regions being well replicated. Thus we can monitor the temporal evolution of magnetic fields from the farside to the frontside of the Sun using SDO/HMI and farside magnetograms generated by our model when farside extreme-ultraviolet data are available. This study presents an application of image-to-image translation based on cGANs to scientific data.

• Journal of dental hygiene science
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• v.22 no.3
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• pp.148-155
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• 2022

Background: The use of dental floss along with a toothbrush is a well-known oral hygiene product that effectively removes dental plaque and reduces the risk of dental caries and periodontal disease. Despite the fact that various types of floss are being used, flossing methods based on the thread type are being taught. In addition, personal preference according to the floss types has not been investigated. In this study, individual preferences according to the floss types were investigated by experiencing various types of dental floss to both floss users and non-users. In addition, the change in intention to use dental floss in the future after flossing experience of non-users was investigated. Methods: General public participated in the individual interview survey (n=419). Subjects responded to the questionnaire after using all of the thread type, Y-type, and C-type floss. All statistics were expressed frequencies and percentages for categorical variables, and the chi-squared test was used to determine the statistical significance of associations between the variables. Results: As a result of the analysis, the preference of C-type floss was highest in both floss users and non-users. The biggest reason for not using dental floss was not knowing the necessity of flossing (36.4%). In addition, both floss users and non-users responded that C-type floss was suitable for flossing in the anterior and posterior regions. The change in the positive future intention to use dental floss after flossing experience of non-users was statistically significantly associated with age. Conclusion: This result suggests that there is a need to provide education on how to use dental floss in various forms. Based on this, it will be possible to lead a change in individual's attitude for future oral health through active and continuous flossing experience.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.1
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• pp.41.2-41.2
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• 2021

In this study, we generate He I 1083 nm images from Solar Dynamic Observatory (SDO)/Atmospheric Imaging Assembly (AIA) images using a novel deep learning method (pix2pixHD) based on conditional Generative Adversarial Networks (cGAN). He I 1083 nm images from National Solar Observatory (NSO)/Synoptic Optical Long-term Investigations of the Sun (SOLIS) are used as target data. We make three models: single input SDO/AIA 19.3 nm image for Model I, single input 30.4 nm image for Model II, and double input (19.3 and 30.4 nm) images for Model III. We use data from 2010 October to 2015 July except for June and December for training and the remaining one for test. Major results of our study are as follows. First, the models successfully generate He I 1083 nm images with high correlations. Second, the model with two input images shows better results than those with one input image in terms of metrics such as correlation coefficient (CC) and root mean squared error (RMSE). CC and RMSE between real and AI-generated ones for the model III with 4 by 4 binnings are 0.84 and 11.80, respectively. Third, AI-generated images show well observational features such as active regions, filaments, and coronal holes. This work is meaningful in that our model can produce He I 1083 nm images with higher cadence without data gaps, which would be useful for studying the time evolution of chromosphere and coronal holes.

• Korean journal of food and cookery science
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• v.23 no.2 s.98
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• pp.195-204
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• 2007

The objective of this study was to analyze dietitians' sanitary practices and perceptions of obstacles according to HACCP. Questionnaires were administered to 94 elementary school dietitians in the Gwangju and Jeonnam Regions of South Korea. The data were evaluated by a 5 scale Likert method and statistically analyzed The results of the study are as follows. Each subject was generally characterized as a university graduate 79.8%, with 10 to 15 years experience 37.6%, and in her mid to late thirties 39.4%. We found that direct management was the highest occurring form of food service system 96.8%. With regards to the type of food service operation quantitative service as 60.6%, and partial self-service was 30.9%, 89.4%of all served only lunch. In all, 93.6% of the teams collaborated, while their organic collaboration was 64.9%. When HACCP was applied, sanitary practices scored an average of 4.83, which was significantly higher than 4.62 when HACCP was not applied. The dietitians' perception of sanitary practices was as very high 4.75 of 5. Sanitation, in regards to food management, scored 4.89 among the detailed items. This proves that in relation to food management, sanitation is considered most important. When HACCP was not applied, the perception of obstacles was, on an average, 3.07, which was higher than 2.74 when HACCP was applied. The results for the detailed items, which focused on sanitary practices and different perceptions of obstacles according to HACCP, were as follows. When HACCP was applied, a high mean of 3.32 occurred for facilities and utilities shortages followed by degree of cooperation at 3.22, and then monitoring at 3.01. The item that dietitians viewed as the biggest obstacle was lack of departmental budget support (M=3.46). Other serious obstacles were facilities, facility sanitation, and utility shortages (M=3.38), as well as the aappropriateness of machines and cooking utensils and arrangements in the dining room (M=3.28). Stepwise multiple regression was used to better understand how much these perceptions of obstacles would influence sanitary practices when HACCP was applied. The item analysis of the HACCP obstacles proved that persons involved in food service, except the food service employees, didn't cooperate enough with their food service employees. As a result, this lack of cooperation had a negative effect on sanitary practices. Accordingly, it is necessary to determine the causes of for lack of cooperation and attract the active attention of those involved.

• Journal of Korea Technology Innovation Society
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• v.10 no.1
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• pp.98-120
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• 2007

This study analyzed the linkage relations of technological innovation between Daedeok Innopolis and other seven innovative clusters in industrial complexes which have played important roles as innovative centers in Korea. To begin with, we selected major technologies of Daedeok Innopolis for which the linkage with other clusters of innovative activities was analyzed quantitatively and graphically with association matrices, network analysis and dendrogram. Based on this network analysis, we analyzed the technological linkage among clusters and found the direction and strength of technological cooperation of the Daedeok Innopolis with other innovative clusters by technology field. This study analyzed the technological network between the Daedeok Innopolis and other clusters on 6 technology fields in which Daedeok has innovative capabilities. In the analysis was measured the centrality of Daedeok compared with that of other clusters in areas where Daedeok is relatively active in innovative activities. As a result, Daedeok showed the highest centrality with the active innovative activities. Except Daedeok, Banwol-Sihwa cluster showed the highest centrality in all of the 6 technology fields. Putting all the centrality of each innovative cluster and the linkage relations of Daedeok Innopolis together, it need to consider the following policy directions to strengthen the innovative linkage with other clusters. In Daedeok's main technology areas, it needs to increase the regional linkage with the clusters with relatively high centrality and relatively low linkage with Daedeok. For Changwon, Wonju and Gunsan with which Daedeok has the relatively low innovative linkage, it need to examine a new policy to improve the linkage with technologies related with the industry other clusters are nurturing. When Daedeok formulates a plan to cooperate with other clusters, it does not only have to consider the linkage relations of technological innovation analyzed in this study and but also has to examine the cooperative strategies from the comprehensive point of view considering every aspect of their strategic industries, supportive policies, industrial base and competitiveness. This study analyzed the linkage relations between regions based on the network analysis of patents only by co-invention. Consequently, it did not examine the factors inducing the co-invention, which is a limitation of the study and is to be investigated in the future. Also, the exploratory analysis on determinants of linkage intensity and the strategies to get synergy effects through solidified linkage will be the issues of further study.

• Molecules and Cells
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• v.44 no.9
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• pp.658-669
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• 2021

Enhancers have been conventionally perceived as cis-acting elements that provide binding sites for trans-acting factors. However, recent studies have shown that enhancers are transcribed and that these transcripts, called enhancer RNAs (eRNAs), have a regulatory function. Here, we identified putative eRNAs by profiling and determining the overlap between noncoding RNA expression loci and eRNA-associated histone marks such as H3K27ac and H3K4me1 in hepatocellular carcinoma (HCC) cell lines. Of the 132 HCC-derived noncoding RNAs, 74 overlapped with the eRNA loci defined by the FANTOM consortium, and 65 were located in the proximal regions of genes differentially expressed between normal and tumor tissues in TCGA dataset. Interestingly, knockdown of two selected putative eRNAs, THUMPD3-AS1 and LINC01572, led to downregulation of their target mRNAs and to a reduction in the proliferation and migration of HCC cells. Additionally, the expression of these two noncoding RNAs and target mRNAs was elevated in tumor samples in the TCGA dataset, and high expression was associated with poor survival of patients. Collectively, our study suggests that noncoding RNAs such as THUMPD3-AS1 and LINC01572 (i.e., putative eRNAs) can promote the transcription of genes involved in cell proliferation and differentiation and that the dysregulation of these noncoding RNAs can cause cancers such as HCC.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.343-354
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• 2014

Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1182-1190
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• 2007

The gene encoding for isoamylase of the Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 was cloned and expressed into Escherichia coli $DH5{\alpha}$. Isoamylase catalyzes the hydrolysis of ${\alpha}-1,6-glycosidic$ linkages specifically in amylopectin, glycogen, and derived oligosaccharides, while the enzyme did not hydrolyze ${\alpha}-1,4-glycosidic$ linkages of amylose. The isoamylase gene (glgX) had an open reading frame of 1,977 bp encoding 658 amino acid residues with a calculated molecular weight of 74,188 Da. The molecular weight of the enzyme was also estimated to be 74 kDa by activity staining of a SDS-PA gel. The mature GlgX had a calculated pI of 4.91. Isoamylase from Pcc LY34 had 70% amino acid identity with isoamylase from Pectobacterium chrysanthemi and contained the four regions conserved among all amylolytic enzymes. The isoamylase was optimally active at pH 7.0 and $40^{\circ}C$. GlgX was $Ca^{2+}-dependent$. The changes of Asp-335, Glu-370, and Asp-442 into Ala, respectively, using site-directed mutagenesis techniques showed that three residues are essential to isolamyalse (GlgX) activity. The sequences around those residues were highly conserved in isoamylase of different origins and GlgX of the glg operon in glycongen biosynthesis.