Thirty strains of genus Agrocybe which had been kept in National Institute of Horticultural and Herbal Science, were tested for the formation of fruiting body. Nineteen strains of collected genus Agrocybe were formed fruiting body at bottle culture of sawdust medium. Five strains (including ASI 19003) and thirteen strains (including ASI 19007) could be grouped as A. cylindracea and A. chaxingu. Although ASI 19008 showed the formation of fruiting body, morphological characteristics were significantly different from the two groups. Cultural period of ASI 19003 strain at sawdust substrates was 29 days and ASI 19007 train was 30 days in liquid spawn inoculation. The yield and quality of ASI 19003 strain was excellent in the spring, fall, and winter, whereas ASI 19007 strain was excellent in the summer. Accordingly, these cultivar might be contribute to farmers' income by stable year-round production if using the season-oriented strain.
Proteins and lipids not only provide a source of energy to the cell, but also play vital roles in modifying the physical properties and function of the biological membranes. In the present study, we investigated the biochemical constituents, viz. proteins and lipids, in growing oocytes of goat antral follicles during summer and winter seasons. Goat genitalia in phosphate buffered saline (pH 7.4) were brought to the laboratory within one hour of slaughter under aseptic conditions at $37^{\circ}C$. Oocytes were aspirated from normal small (<3 mm in diameter) and large (>3 mm) follicles and pooled for biochemical estimations. A significant increase in the amount of protein and lipid was observed with the growth of the oocyte. The amount of protein varied non-significantly with the season, while the amount of lipid varied significantly. The amounts of phospholipid, cholesterol, free fatty acid, and triglyceride increased with the growth of the oocyte, but no significant effect of season in these constituents was observed. Lysolecithin, sphingomyelin, and sterols were the polar lipids identified in both oocytes prepared from small follicles (small oocytes) as well as large follicles (large oocytes). In addition, the small oocytes also contained phosphatidyl serine, while large oocytes contained phosphatidyl glycerol phosphate and phosphatidyl inositol. Among non-polar lipids, triglycerides and long chain alcohols appear only in small oocytes and not in large oocytes. Monoglycerides, 1,2-diglycerides, 1,3-diglycerides and o-dialkyl glycerol ethers, fatty acids, fatty acid methyl esters, and wax esters were identified in both small and large oocytes. Information on biochemical composition of growing oocytes is relevant to oocyte and embryo competence, culture and cryopreservation.
Changes in the bacterial populations of a 5-stage biological nutrient removal (BNR) process, with a step feed system for wastewater treatment, were monitored by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA fragments. DGGE analysis indicated seasonal community changes were observed, however, community profiles of the total bacteria of each reactor showed only minor differences in the samples obtained from the same season. The number of major bands was higher in the summer samples, and decreased during the winter period, indicating that the microbial community structure became simpler at low temperatures. Since the nitrogen and phosphate removal efficiencies were highly maintained throughout the winter operation period, the bacteria which still remaining in the winter sample can be considered important, playing a key role in the present 5-stage BNR sludge. The prominent DGGE bands were excised, and sequenced to gain insight into the identities of the predominant bacterial populations present, and most were found to not be closely related to previously characterized bacteria. These data suggest the importance of culture-independent methods for the quality control of wastewater treatment.
The authors performed clinical and mycological studies on Tinea cruris of 842 outpatients who had visited the dermatology clinic of Seoul National University Hospital from 1975 to 1979. 1) The incidence of Tinea cruris was 4.4% in maximum and 3.0% in minimum of the total superficial mycotic diseases cases, so there are no remarkable changes of the yearly prevalence rate. 2) Tinea cruris was more frequent among males, and the male cases were 15.2 times higher than those of female cases. 3) By the monthly distribution, Tinea cruris shows most high in summer season (June, July and August). 4) The age distribution group for Tinea cruris varied from the first to the eighth decade, but most of them were in their third decade. 5) Concurrent infection of Tinea cruris with other types of dermatophytoses(Tinea) was noted in 19.0% of the cases. Patients with Tinea cruris and Tinea pedis were most highly observed in 51.3%, and on next. Tinea cruris and Tinea corporis 25.6% in all of the concurrent infection cases. 6) The strains were identified by culture on ordinary Sabouraud's glucose agar media and abtained three species and 99 strains. a) Trichophyton rubrum was most common causative organism of Tinea cruris. Trichophyton rubrum was isolated 64 strains (64.6%). b) Trichophyton mentagrophytes was isolated 27 stains (27.3%) and Epidermophyton floccosum was 8 strains (8.1%).
Upper temperature tolerance of the turbot Scophthalmus maxim us, one of the popular aquaculture species in European community and China, was evaluated in terms of survival and growth at $20^{\circ}C$, $23^{\circ}C$, $26^{\circ}C$, or $29^{\circ}C$. Best growth was achieved at temperature $20^{\circ}C$ in this experiments. The fish exposed to $20^{\circ}C$ or $23^{\circ}C$ were comparable in survival, condition factor and feed conversion efficiency reminiscent of the latter temperature to be agreeable for the fish. The temperature over $23^{\circ}C$ appeared to be the temperatures beyond the fish can tolerate. For instance, the fish exposed to 26 showed mortality of 60.9% by day 60; none of the fish exposed to $29^{\circ}C$ survived beyond day 7. Culture densities between 80 and $200\;fish/m^2$ did not influence the survival, growth, condition factor and specific growth rate of the fish. The final production of the culture density experiment was $10\;kg/m^2$ on average. These results imply that the location where water temperature remains lower than $25^{\circ}C$ during summer can be a candidate site for the turbot aquaculture.
In this study, nine scarf designs expected to be effective for accent design for the fashion style of new senior women were suggested by reflecting purchase behavior and scarf preference after conducting surveys and an analysis by targeting 136 new senior women in order to propose scarf design-matching with the preferences of new seniors. As a result of the study on the scarf purchase features of new seniors, it was revealed that the purchase time for scarves was mainly autumn and winter, even though it is regardless of season, and in spring and summer, they seldom purchased scarves. The purchase frequency was four times a year, and what they first thought of at the time of purchase was represented in the order of color, design, and the material of the scarf. They most preferred department stores, mixed and achromatic colors, cotton and silk fabrics, natural and geometric patterns, and long scarves of a rectangular shape. In the case of the consumer attributes of scarves, it was revealed that $50^{th}$ desired more individuality-oriented, unique scarf designs than $60^{th}$, and $60^{th}$ desired scarf designs with convenient management considering others' attention compared with $50^{th}$. As concepts for scarf designs, the aspects of individuality, co-existence, and maturity were extracted by reflecting the features of new senior women, and a total of nine scarf designs were suggested by developing three sub-designs for each concept.
Kim, Chul Ki;Choi, Yong-Jin;Lee, Chul-Woo;Rim, Yeon-Taek;Ryu, Jae-Keun
Microbiology and Biotechnology Letters
/
v.25
no.1
/
pp.89-95
/
1997
The current processer of the textile wastewater treatment are mostly consisted of a combination of a physico-chemical and a biological treatment. The overall efficiency of these processes is, however, assessed to be fairly low. It is even worse during the summer season when temperature of the wastewater rises above 40$\circ $C. Therefore, a feasible process of the textile wastewater treatment which can work efficiently at higher temperatures was investigated in this work. We used a bench scale reactor consisted of one 4 liter anaerobic and one 8 liter aerobic tank, and the thermophilic symbiotic PVA degraders, Pasteruella hemolytica KMG1 and Pseudomonas sp. KMG6 that had been isolated in our laboratory. In the preliminary flask experiments, we observed that the thermophilic symbiotic PVA degraders could not grow in the wastewater substrate. Then, we isolated the mutant strains by acclimating the KMG1and KMG6 strains to the wastewater medium. The mutant symbionts (KMG1-1 and KMG6-1) were isolated through 6 times successive transfers into the fresh wastewater medium after 5 days culture for each. The mutant strains obtained grew well in the mixed medium composed of 75% wastewater and 25% synthetic medium, and supplemented with 0.5% PVA as a sole carbon source. During the culture for 14 days at pH 7.0 and 40$\CIRC $C, the bacteria assimilated about 89% of the added PVA. The symbionts degraded equally well all the PVA substrates of different molecular weight (nd=500~30000). In contrast to the flask experiments, in the reactor system the mutant strains showed very low levels of the PVA and COD removal rates. However, the new reactor system with an additional aerobic tank attained 82% removal rate of COD, 94% of PVA degradation and 71% of color index under the conditions of 5% inoculm on the tank 2, incubation temperature of 40$\circ $C, dissolved oxygen level of 2~3 mg/l and retention time of 30 hours. This result ensures that the process described above could be an efficient and feasible treatment for the PVA contained textile wastewater at higher temperatures.
Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC) of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964 and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest in TMM and MYA at $25^{\circ}C$. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing inside soil and walls of a few inoculation sites, while mycelial mats growth up to $5{\sim}8$ cm were observed in the roots of pine tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation, no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake. The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the appropriate environmental conditions.
The main focus of this paper is to provide clear understanding about the concept of make-up as total fashion by defining the essential meaning of it. First, we would like to go over the concept of make-up and total fashion, then analyze the make-up image, the changes of colors and images of make-up overtime and fashion that have influenced over those changes. In this paper, we analyze the trend of fashion and make-up in 1990s and their relevancy. Through this paper we hope that make-up can be accepted as a part of total fashion in its relationship with other elements such as shoes, clothes and accessory and that it can be considered as a independent art that has direct influence on people. The trend of Spring/Summer between 1990 and 92is the image of natural beauty and comfortable and modernistic image that can be experienced through nature. In 1992, there was a revival look of 1960s and 1970s. Make-up also followed this trend. The overall color tone was to give natural and stable images with pastel tone to soften the images. This tone brought back the ecology of 1960s. The theme of restoration in humanity and nostalgia was reflected in clothing, and these trend changed the ecology of make-up to peace, love and romantic ecology which was expressed in coral, blue, and green tone to create intelligent image of woman. Year 1996 could be called as color revolution period that emphasized the unique and individual expression of each person. In 1997, black, pastel and brown colors were the result of reinterpreting the classic and sexy images of 1960s to natural and modernistic image of 1997. Purple color started to be introduced to us. The beginning of 1990s Fall/Winter season was based on ecology concept that emphasized the natural image. Until 1995 it appeared that spring/summer and fall/winter trend had no big differences. But from 1995 seasonal differences in trend are appeared and there were various make-up designs. In 1995, 1996, brown color lines make-up comes to mix with romantic image and developed into wine, orange, neon colors. These color were the symbol of property and sentiment and gold make-up emphasizing the eye area was the tendency of that period. In 1997, the fear of coming end of century was expressed as decadent image and at that time ethnic image, romantic image appeared with vivid color lines, gold, red and violet.
Lee, Jong Nam;Kim, Hye Jin;Kim, Ki Deog;Yoo, Dong Lim;Suh, Jong Taek
Horticultural Science & Technology
/
v.32
no.5
/
pp.739-743
/
2014
This work characterizes 'Yeolha', a new strawberry (Fragaria ${\times}$ ananassa Duch.) cultivar that was released by the Highland Agriculture Research Center, RDA, in 2013. This cultivar originated from a 2008 cross between 'Goha' and 'Elsinyo' and showed excellent ever-bearing characteristics including continuous a flowering habit under long-day and high temperature conditions. It was originally named 'Saebong No. 5' after examining its characteristics and productivity in summer culture from 2010 to 2013. After two regional adaptability tests in 2012, it was selected as an elite cultivar and renamed 'Yeolha'. The general characteristics of 'Yeolha' include a semi-spreading plant type, elliptic leaves, and strong vigor in growth. Its fruit is conical and red. T he s oluble s olids content of 'Yeolha' is 8 .6%, w hich is 0.6% h ig her than t hat of 'Flamenco', b ut f ruit hardness is lower than that of 'Flamenco'. The average fruit weight of 'Yeolha' is about 12.1 g and the marketable yield is $28,133kg{\cdot}ha^{-1}$, which is 117% higher than that of 'Flamenco'. 'Yeolha' is resistant against Fusarium wilt. In addition, 'Yeolha' is suitable for four-season cultivation as a high harvesting cultivar because it shows continuous flowering under long-day and high temperature conditions.
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