• Journal of Animal Science and Technology
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• v.64 no.5
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• pp.911-921
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• 2022

Maximum residue limits (MRL) for pesticides in feed have been set to protect public health and produce safe livestock products. In vivo experiments to establish MRL are essential, as livestock are commonly used to obtain reliable in vivo quantitative information. Here, we aimed to evaluate whether small laboratory animals can replace or reduce monogastric livestock in experiments to quantify pesticide residues in vivo after oral consumption through feed. First, 24 pigs and rats were randomly assigned to four groups and fed 0, 3, 9, or 30 mg/kg of sulfoxaflor. After four weeks, serum, muscle, fat, liver, kidney, and small intestine samples were collected, and sulfoxaflor residues were analyzed using liquid chromatography - tandem mass spectrometry. Sulfoxaflor residues in pig tissues were significantly correlated with those in rat tissues. Model equations were formulated based on the residual sulfoxaflor amount in pig and rat tissues. The calculated and measured sulfoxaflor residues in pigs and rats showed more than 90% similarity. Sulfoxaflor did not affect body weight gain, feed intake, or the feed conversion ratio. Therefore, we concluded that pesticide residue quantification in vivo to establish MRL could be performed using small laboratory animals instead of livestock animals. This would contribute to obtaining in vivo pesticide residue information and reducing large-scale livestock animal experiments.

• Journal of The Korean Society of Clinical Toxicology
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• v.13 no.1
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• pp.43-45
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• 2015

Sulfoxaflor is the first insecticide belonging to the sulfoximine class and is efficient against sap-feeding insects that are resistant to other insecticides. Sulfoxaflor acts as a neurotoxin to the central nervous system of insects compared with very low toxicity to mammalian. We report on a case of a 67-year-old male who ingested insecticide and received conservative treatment for mild metabolic acidosis and gastrointestinal symptoms.

• Korean Journal of Food Science and Technology
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• v.45 no.2
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• pp.148-155
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• 2013

Sulfoxaflor is a new active ingredient within the sulfoximine insecticide class that acts via a unique interaction with the nicotinic receptor. The MRLs (maximun residue limit) of sulfoxaflor in apple and pear are set at 0.4 mg/kg and that in pepper is set at 0.5 mg/kg. The purpose of this study was to develop an analytical method for the determination of sulfoxaflor residues in agricultural commodities using HPLC-UVD and LC-MS. The analysis of sulfoxaflor was performed by reverse phase-HPLC using an UV detector. Acetone and methanol were used for the extraction and aminopropyl ($NH_2$) cartridge was used for the clean-up in the samples. Recovery experiments were conducted on 7 representative agricultural products to validate the analytical method. The recoveries of the proposed method ranged from 82.8% to 108.2% and relative standard deviations were less than 10%. Finally, LC-MS with selected ion monitoring was also applied to confirm the suspected residues of sulfoxaflor in agricultural commodities.

• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.219-228
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• 2019

This study summarized processing factor (PF) by the stage of rice and barley processing based on JMPR reports from 2006 to 2016. We compared PF of 17 pesticides in rice products during the processing of rice grain, husked rice, polished rice, hulls, bran and cooked rice. Among the 17 pesticides, 12 pesticides except for 5 pesticides such as acephate, methamidophos, glufosinate, quinclorac and sulfoxaflor mostly decreased in pesticides when rice grain processed into brown rice. Pesticides tended to be partially reduced when processed from husked rice to polished rice. However, hulls and bran produced during the milling process were concentrated. Acephate and others, 5 pesticides are systemic pesticides, and pesticides are penetrated into foods, and a large amount of pesticides was not removed during the milling process. The remaining pesticide residues in polished rice were mostly removed after processing into cooked rice, and trace amounts of pesticide residues remained. In the comparison of 23 pesticides PF during the processing of barley products (pearl barley, flour, short, malt, beer, hulls and bran). Most of the pesticide except for 4 pesticide (ethephon, pyraclostrobin, penthiopyrad, sulfoxaflor), which are systemic pesticides, decreased during the process of pearl barley production out of the barley grain. The pesticide in the malt, which was made by steeping pearl barley was concentrated but when processed into beer, pesticide was remained only in trace amounts.

• The Korean Journal of Pesticide Science
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• v.20 no.2
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• pp.133-137
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• 2016

In Korea, six mealybug species have been reported on pears. This study investigated the occurrence of mealybugs in 19 pear orchards from 2013 to 2014. Two species, Crisicoccus matsumotoi Siraiwa (Hemiptera: Pseudococcidae) and Pseudococcus comstocki Kuwana were mainly found. The dominant species was C. matsumotoi identified on 73% of infested fruit. Toxicities of 14 registered insecticides (Buprofezin+Acetamiprid EC, Buprofezin+Amitraz EC, Buprofezin+Clothianidin SC, Buprofezin+Dinotefuran WP, Buprofezin+Etofenprox WG, Buprofezin+Thiacloprid SC, Buprofezin+Thiamethoxam SC, Benfuracarb WG, Acetamiprid WP, Clothianidin SC, Dinotefuran WG, Thiacloprid SC, Thiamethoxam WG, Sulfoxaflor SC) commonly used to control P. comstocki were evaluated to C. matsumotoi nymphs and adults at the recommended concentration. As a results, all insecticides exhibited strong insecticidal activity with 100% mortality of both nymphs and adults. These results indicate that the 14 insecticides can be used in control for C. matsumotoi in field.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.139-147
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• 2023

Leaf-spray in vitro bioassays appraise new aphicidal formulations for managing deleterious plant-feeding aphids. The formulation may utilize alternative and integrated strategies. However, leaf spraying even under controlled conditions may affect aphid reproduction and mortality. This study examines leaf spray applications for optimum and reproducible aphicidal results using tobacco leaves overlaid on cotton fabric or water agar surfaces. Infestation of the undersides of tobacco leaves with nymphs of green peach aphids was used in the assays. Spray distance and volume were optimized using water-sensitive paper to ascertain the best surface coverage. Overlays of the leaves on water agar caused less mortality and greater reproduction than the use of cotton fabric. The relative humidity of the insect-rearing chambers changed with the watering regime for the insect - rearing chambers with cotton fabric; 60% relative humidity was optimal. Relative humidity was not affected by the concentration of agar in the water agar chambers. Applications of the chemical aphicidal standard, Sulfoxaflor, under the optimized conditions exhibited similar times for lethality although the rate was faster with leaves on the cotton fabric than on water agar. These studies establish reproducible and sensitive techniques for assessing the lethality and effects on reproduction of potential aphicidal products.

• Korean journal of applied entomology
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• v.62 no.4
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• pp.267-275
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• 2023

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of insecticidal active ingredients in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of active ingredients, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan-Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.271-280
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• 2019

We investigated the control efficacy and phytotoxicity of unmanned aerial vehicle-applied pesticides against the insect Spodoptera exigua, a major emerging pest in Chinese cabbage. Phytotoxicity was investigated in cabbage and 7 crops cultivated in the perconducted on 8 surrounding crops including Chinese cabbage at 1 to 2 times the recommended pesticide dosage. We treated cabbage fields with spinetoram suspension concentrate (16×), methoxyfenozide, sulfoxaflor suspension concentrate (16×). Then, we used water-sensitive paper to measure the distribution pattern of falling pesticide particles and the degree of coverage. Two of the pesticides showed 97% control efficacy, however, control efficacy might differ in resistant populations. Phytotoxicity was not observed in Chinese cabbage and the 7 surrounding crops treated with 1 to 2 times the recommended pesticide dosage. Analysis of the distribution pattern of falling pesticide particles revealed that breeze caused particle diffusion. Thus, wind is an important factor affecting the uniform treatment and diffusion of multicopter-applied pesticides. It follows that setting optimal conditions is necessary for effective control and treatment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.231-236
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• 2021

The Aphid, Myzus persicae, and the bean bug, Riptortus clavatus, are major insects in crops. This study examined the insecticide susceptibility and phytotoxicity of insecticides dispersed using an Unmanned Aerial Vehicle (UAV, multi-copter) against the insects. Sulfoxaflor suspension concentrate (SC, 16X) on potato fields and etofenprox, methoxyfenzide suspo-emulsion(SE, 8X) on soybean fields were dispersed after deploying water-sensitive paper within the field to measure the distribution pattern and coverage index of the falling insecticide. Both insecticides showed a controlled mortality of 76.4% against aphids and 97.5% and 94.4% against the 2nd nymphal, and 5th nymphal stage of the bugs, respectively. The droplet distribution was less than 0.5mm, and coverage analysis revealed an inside and outside coverage of 3.1 and 1.6, respectively. The surrounding area was affected by insecticide spraying using a multi-copter. This study is expected to help expand UAV control and use it safely in the future.