• 한국수산과학회지
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• 제40권2호
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• pp.68-72
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• 2007

The flow properties of sulfated polysaccharides purified from the ascidian Halocynthia roretzi tunic were investigated. The sulfated polysaccfarides produced aqueous solutions of low apparent viscosity with pseudoplastic flow behavior. The respective activation energies of TCA-treated and TCA-nontreated solutions were $2.2248{\times}10^4\;and\;1.442{\times}10^4J/kg{\cdot}mol$ at a 150L/s shear rate. The viscosity of the sulfated polysaccharides solution was increased by the addition of sugar, while it was not changed by the addition of NaCl.

• 한국해양생명과학회지
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• 제2권1호
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• pp.7-11
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• 2017

Sulfated polysaccharides are known to be immune-stimulators in mammals and can be used as food additives to enhance immunity. In this study, the immune-stimulating activity of water-soluble anionic macromolecules F₂ fractionation isolated from Codium fragile using ion-exchange chromatography was tested in olive flounder, Paralichythys olivaceus, in vitro and in vivo. The gene expression of interleukin (IL)-1β was adopted to check the immune-affection. As a result, in vitro study revealed that the expression of IL-1β was significantly upregulated in head kidney cells by 1 and 5 ㎍/ml of polysaccharides 4 h and by 5 ㎍/ml of polysaccharides at 24 h. In vivo, IL-1β gene expression in head kidney was significantly upregulated by 20 and 100 ㎍ of the polysaccharides at day 1 post-i.p. injection, while downregulated at day 3 but not significant. Meanwhile, in peritoneal cells, it was upregulated by 20 ㎍ of the polysaccharides at day 1 but the upregulation was sustained until day 3 though it was not significant. These results indicate that the sulfated polysaccharides from C. fragile are an immune-stimulator and might be potential feed additives for olive flounder.

• 생약학회지
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• 제9권4호
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• pp.157-167
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• 1978

Atherosclerosis is associated with the presence of extracellular lipid droplets and large fatty deposits, both of which are to be covered at the surface mainly by zwitterionic phospholipids. The development of atherosclerosis is often associated with the accumulation of calcium. Furthermore, the presence of glycosaminoglycans directly underlying fatty deposits in human aorta has been demonstrated. Also, the possible involvement of the interaction between sulfated polysaccharide and lipoprotein in the development of atherosclerosis has been suggested in view of the presence of both low density lipoproteins and glycosaminoglycans, as well as their complexes, in atherosclerotic aortas. Therefore interactions of sulfated polysaccharides with low density lipoproteins which serve as a vehicle for cholesterol and cholesterol ester and with zwitterionic phospholipids have been studied extensively by a number of workers to provide mechanisms. In this paper, the mechanism of the interaction of sulfated polysaccharides with low density lipoproteins and the mechanism of the interaction between sulfated polysaccharides and zwitterionic phospholipids are reviewed. The possibility of the occurence of these interactions in the body are also considered.

• 약학회지
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• 제36권4호
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• pp.350-356
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• 1992

Anticoagulant activities were tested for the fifteen kinds of medicinal plants by measuring activated partial thromboplastin time (aPTT). Of them five kinds or species (Artemisia princeps, Sanguisorba officinalis, Artemisia apiacea, Eclipa alba, Schizonepeta tenuifolia) were selected and fractionated for the preparation of acidic polysaccharides. They were extracted with water by refluxing and the extracts were precipitated with ethanol. The precipitates were separated based on charge using a DEAE-Sephadex. The low salt and high salt fractions were sulfated with anhydrous pyridine and chlorosulfonic acid complex. In vitro anticoagulant activities of sulfated polysaccharides were tested by measuring aPTT, prothrombin time (PT), and factor Xa clotting time using normal human plasma. No relationship was found between the amount of uronic acids and anticoagulant activities, but the sulfated ones show the increase of activities. In vivo anticoagulant properties of the sulfated polysaccharide from Artemisia apiacea were also tested by the intraveneous administration of three different doses (3,5 and 10 mg/kg) to rats. APTT and PT were increased significantly and the action of factor Xa and thrombin mediated through antithrombin III were inhibited slightly.

• Ocean and Polar Research
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• 제38권2호
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• pp.129-137
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• 2016

This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by $CaCl_2$ (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about $1.84{\times}10^3$ and $1.42{\times}10^3kDa$, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance Anion-Exchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2002년도 학술발표대회
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• pp.31-31
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• 2002

• ALGAE
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• 제38권4호
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• pp.217-240
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• 2023

Sulfated polysaccharides (SPs) isolated from seaweed have emerged as remarkable bioactive compounds with a wide spectrum of biological activities and have substantial value in the scientific and industrial domains. The current study explores the diverse biological activities of SPs and their relationship with their structures. This aids in an in-depth examination of the multifaceted biological activities of SPs, including anticoagulant, anti-inflammatory, antiviral, antioxidant, and immunomodulatory properties, which underpin their potential health benefits. Furthermore, the current study explores the complicated properties of SPs, with their extraction methodologies and techniques for precise characterization. Elucidation of the commercial significance of SPs derived from brown, red, and green seaweed by highlighting their potential applications has emphasized their importance in human well-being. Further, this review emphasizes the challenges needed to overcome research and industrial innovations for SPs. Collaboration among researchers, industry stakeholders, and regulatory authorities can overcome these challenges and elevate the potential of SPs to revolutionize industries such as pharmaceuticals, cosmeceuticals, food, and biotechnology.

• 한국수산과학회지
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• 제31권3호
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• pp.447-451
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• 1998

우렁쉥이 (Halocynthia roretzi)의 껍질에서 최근 다양한 생리적 기능이 알려진 황산화다당를 분리하여 화학성 분과 조성 당을 분석하였으며 , 기능성을 살펴보았다. 황산화다당의 황산기함량은 $2.3\%$, 황산콘드로이틴은 $25.7\%$, 그리고 uronic acid의 함량은 $5.6\%$였다. 조성당 분석결과, 5탄당으로는 arabinose $0.7\%$와 xylose가 $0.4\%$였고, 6탄당으로는 glucose가 $1.5\%$였으나. 특이하게 galactose의 함량이 $60\%$이상 검출되었다. 한편, hexosamine으로는 N-acety1gucosamine이 $9.4\%$ 및 N-acetylgalactosarnine이 $14.8\%$ 검출되었으며, uronic acid로는 D-glucuronic acid가 $10.8\%$ 검출되었다. 지방흡수력은 대조구인 chitin과 chitosan에 비하여 다소 떨어졌으며, 추출한 황산화다당의 지방흡수력은 대략 $280\%$ 정도였다. 한편 포말성 및 포말안정성은 각각 0.70 과 0.50으로 비교적 우수하였다. 유화능 및 유화안정성은 $49.2\%$와 $48.3\%$ 정도였으며, 황산화다당의 항혈액응고능은 APTT가 65초로 저지효과가 인정되었으나, PT저지효과는 없었다. ACE저해능의 측정 결과. 약간의 ACE저해효과가 있었으며, 채택된 실험방법에 따라 동일시료의 경우에 있어서도 저해효과 값이 차이가 있었다.

• 한국수산과학회지
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• 제34권6호
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• pp.632-637
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• 2001

우렁쉥이 껍질에서 분리한 황산다당을 부분정제하여 몇 가지 화학조성과 전기영동을 하였다. 추출된 황산다당의 화학적 조성은 sulfate, uronic acid, protein, chondroitin sulfate, amino sugar, hexosamine 등으로 이루어져 있었다. 전기영동결과 autoclave 처리에 의해 추출된 조다당류는 폭넓은 단일 band를 나타내었으나, neutrase 처리구는 세 개의 band로 구분되었다. Autocalve 처리구의 분자량은 약 40,000 정도로 추정되며, neutrase 처리구의 경우 고분자 band는 100,000 이상, 두 개의 저분자 band 중 하나는 약 22,000 정도, 또 하나의 band는 약 5,000 정도인 것으로 추정되었다. 이온교환수지 및 겔 여과하여 얻은 전당획분의 화학적 조성은 부분정제 전과 거의 유사하였다. Sephadex G-25로 gel 여과한 시료의 조성당은 arabinose, xylose 및 glucose는 흔적량 정도이었으나 galactose는 $71.4\%$로 대부분을 차지하였고, N-acetylgalactosamine 함량이 $14.5\%$ 정도이었고, D-glucuronic acid가 $6.3\%$ 정도 검출되었다. Sephadex G-100과 G-100-25로 정제한 당의 전기영 동 패턴은 거의 유사하게 단일 band의 양상이나 G-25 처리구가 다소 좁은 band를 나타내었다. 부분정제한 황산다당과 표준품인 chondroitin sulfates의 FT-IR spectrometer 분석 결과, 거의 유사한 결과를 나타내었다. 황산기나 에스테르 등 몇몇 중요한 원자단의 신축 진동수가 IR spectrum의 지문영역 ($500\sim700cm^{-1}$$)에서 나타났다. 황산기 중의 S=O 신축진동이 $1,240cm^{-1}$에서, C-O-S신축진동의 경우 수평향배는 $820cm^{-1}$에서 나타났다.

• 한국수산과학회지
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• 제35권6호
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• pp.671-675
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• 2002

우렁쉥이 껍질에서 추출한 autoclave 추출구와 neutrase 추출구는 지방흡수력의 경우 대조시료인 chitin과 chitsan에 비해 떨어지는 대략 250$\~$$300\%$ 정도이었으나, 포말성은 각각 0.70과 0.67로, 포말안정성은 0.50과 0.38로 우수하였다 Autoclave 추출구 및 neutrase 추출구의 유화능은 각각 $49.2\%$와 $47.1\%$, 유화안정성의 경우 각각 $48.3\%$와 $44.8\%$ 정도였으며 특히, 색소흡착능은 각각 $85.2\%$와 $85.6\%$로 대조시료에 비해 다소 우수하였다 항혈액응고능은 모든 시료에서 hPn 저 지효과는 나타났으나 PT값은 약 14.5초로 일정하여 혈액응고저지 효과가 거의 없었다. 특히 autoclave 처리구를 DEAE-cellulose ion exchange chromatography하여 얻은 F4 (획분4)는 APTT가 75초로 가장 효과적 이 었으며, Sephadex G-100의 $F_2$가 70초 정도였다. ACE저해능은 모든 시료에서 ACE 저해효과가 있었으며, 채택된 실험방법에 따라 동일시료 경우에 있어서도 저해효과 값이 차이가 났으며, 특히 DEAE-cellulose 이온교환 처리한 $ F_4$ (획분4)가 $35.7\%$로 가장 효과적이었다.