• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.201-207
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• 2000

The freshwater green alga Chlorococcum sp. grew on NH_4^{+},{\;}NO_3^{-}$, urea, yeast extract, and peptone as the nitrogen source showing similar pattens of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction fo SC was urea. The dffects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were stydied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM phosphate, 1.6 mM sulfate, 170 mM NACl, and $50{\;}\mu\textrm{M}$ iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improned SC prodution by manipulating the nutrient levels in the modified media were descussed.

• Food Engineering Progress
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• v.14 no.1
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• pp.65-74
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• 2010

Culture conditions of L. plantarum TJ-LP-002, the garlic resistant strain isolated from pakimchi (green onion kimchi), were investigated for the use of feed additives. Acetic acid, citric acid, lactic acid, and tartaric acid were detected in the culture supernatant, and especially the concentrations of lactic acid and acetic acid significantly increased during cultivation. The antimicrobial activity of L. plantarum TJ-LP-002 was not affected by proteases, calatase or cellulase, which showed that the antimicrobial activity might be due to the production of acids rather than proteinaceous antimicrobial substances. L. plantarum TJ-LP-002 was resistant to neomycin sulfate, spectinomycin dihydrochloride, and lincomycin hydrochloride, sensitive to streptomycin sulfate, and intermediate resistant to ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, and kanamycin sulfate. The optimum initial pH of medium, fermentation temperature and time for the cell growth and antibacterial activity were pH 7.0, 30${^{\circ}C}$ and 24hr, respectively. The optimal composition of culture medium for the cell growth and antimicrobial activity was 3%(w/v) glucose as a carbon source, 3%(w/v) yeast extract as a nitrogen source, and manganese sulfate and ammonium citrate as inorganic salts. The combinatorial supplementation of these inorganic salts, rather than sole addition as an inorganic salt, resulted in better antibacterial activity.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.205-211
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• 1987

Cephalosporium acremonium MAR-80, a strain of methionine analogue-resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using $Na_{2}^{35}SO_{4}$ as a tracer in the resting cell system. From this result, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low actibity of methionine uptake, which was operated by somewhat simple diffusion. From these studies, it could by anticipated that the improved productivity of cephalosporin C and lower dependence of cephalosporin C production on methionine were related to increased uptake rate of sulfate.

• Economic and Environmental Geology
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• v.28 no.3
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• pp.243-249
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• 1995

In order to investigate the origin of sulfate in rain waters and to evaluate the feasibility of using sulfur isotope method as a tracer of atmospheric pollutants, the sulfur isotopic ratio of sulfate in rain waters collected in Chonju city from October 1994 to March 1995 was monitored and was compared with those of possible sources proposed by previous works. The pH of rain waters shows an intermediate acidic range from 4.45 to 6.88 and their daily variation appears to be well correlated with to the amount of precipitation. The sulfur isotopic ratios of sulfate in rain waters show a highly restricted range from 0.0 to + 1.8‰. The ${\delta}^{34}S$ values are similar to those of soil and pine tree surrounding Chonju city, but largely deviate from those of China. D-parameter($d={\delta}D-8{\delta}^{18}O$) of rain waters varies from 9.4 to 28.8. The values indicate that the rain waters in Chonju city are originated from the rainy front of China continent. All data obtained from this study suggested that sulfate in the rain waters collected in Chonju city was mainly derived from the sulfur dioxide gas emitted by the petroleum combustion. Therefore, sulfur isotopic study for the precipitation provided an excellent tool for environmental assessment in this region and for tracing the source of atmospheric pollutants.

• Korean Journal of Food Science and Technology
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• v.5 no.4
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• pp.224-230
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• 1973

The culture used wheat bran as media for four kind of mold strains such as Aspergillus oryzae, Aspergillus kawachii, Aspergillus usamii and Rhizopus javanicus to examine which strain could higher the activity of amylase most which is used for alcohol fermentation. It also provided three different kind of wheat bran media containing starch of 47%, 51% and 55% respectively for each strain. For each media it also added three different kind of nitrogen sources; ammonium sulfate, casein, and ammonium sulfate and casein equally mixed. Each nitrogen source added was subordinately differentiated into three different percentages, 2%, 4% and 6% respectively, except the 2% for the ammonium sulfate. The results obtained were summarized as follows (1) The activity of ${\alpha}-amylase$ was highest in the media of starch value 47% of wheat bran with 6% of casein added. (2) The activity of ${\beta}-amylase$ was highest in the media of starch value 51% of wheat bran with 2% of the equal mixture of ammonium sulfate and casein added. (3) The activities of both ${\alpha}-amylase$ and ${\beta}-amylase$ of Aspergillus usamii were highest in the media of starch value 47% wheat bran with no addition of nitrogen source. (4) Of the four strains examined, the activities of ${\alpha}-amylase$ and ${\beta}-amylase$ cultured in Rhizopus javanicus were both relatively higher. (5) The activities of ${\alpha}-amylase$ and ${\beta}-amylase$ of the strains examined became lower as the percentage of starch contents increased except in Rhizopus javanicus.

• Journal of Korean Society for Atmospheric Environment
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• v.2 no.2
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• pp.66-74
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• 1986

Rain samples were collected at 10 sites in the Seoul area during the period of August through November, 1985. THe concentrations of the major cations $(H^+, Ca^{++}, MG^{++}, Na^+, K^+, NH_4^+)$ and the major anions $(SO_4^=, NO_3^-, Cl^-)$ were measured to characterize the main sources of chemical ions in rainwater. Correlating concentrations of ions to pH, calculated coefficients ranged from 0.1485 to 0/4296. Sulfate shows the largest coefficient indicating that sulfate is more closely associated with hydrogen ion than other ions. This may suggest that sulfuric acid contributes more to the acidity of rainwater in Seoul. It appears that the major chemicals measured in rainwater are from the anthropogenic sources of air pollution. Predominant chemicals are acidic at the Guro-, the Sinseol-, the Yangnam-, and the Ssangmun-dong with sulfate being the most predominant. IT also indicates that alkaline substances resulting from soil and dust have a significant effect on pH values of rainwater by neutralizing actions. According to Granat-model analysis, it is estimated that the relative contributions to the rainwater acidity in Seoul are 84% from sulfuric acid, 8% from nitric acid and 8% from hydrochloric acid.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.90-93
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• 2003

Sampling of waters from each stage of treatment system, SAPS (Successive Alkalinity Producing System), and spring water near the Hanchang coal mine of Kangwon. Province were carried out periodically and analyzed to evaluate the source and possible path of groundwater contamination by acid mine drainage(AMD). Chemical and sulfur isotope compositions showed that spring water was affected by seepage from mine tailings, and seepage of stonewall, a part of treatment system, was affected by both seepage from mine tailings and mine adit drainage. Through the treatment system no appreciable decrease of sulfur content was identified. And almost similar sulfur isotope compositions of water from each stage of the treatment system may suggest incomplete or very poor sulfate reduction by sulfate reducing bacteria.

• Atmosphere
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• v.20 no.3
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• pp.221-228
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• 2010

Aerosol particles with different size-cuts ($PM_{10}$, $PM_{2.5}$, and $PM_{1.0}$) were collected at Gosan Superstation on Jeju Island from August 2007 to June 2008. Mean concentrations of $PM_{10}$, $PM_{2.5}$ and $PM_{1.0}$ were $29.28{\mu}gm^{-3}$, $17.83{\mu}gm^{-3}$, and $14.30{\mu}gm^{-3}$, respectively. Soluble ions comprised 45.7%, 53.9%, and 60.3% of the total mass of $PM_{10}$, $PM_{2.5}$, and $PM_{1.0}$, respectively. While sulfate was the most dominant species of fine mode ($PM_{1.0}$), nitrate was enriched in coarse mode ($PM_{1.0-10}$). When the concentrations of coarse mode particles were greatly increased, nitrate tended to be enhanced in coarse mode with high calcium but low sulfate concentrations. During the high $PM_{1.0}$ events, however, nitrate was increased with sulfate at fine mode. Particularly, nitrate concentrations were substantially enhanced during high particle episodes, leading high ratios of nitrate to sulfate in air under northwest wind during wintertime. On the other hand, the levels of nitrate were lower than those of sulfate at average particle concentrations. The backward air mass trajectories indicated that nitrate concentrations were elevated in air arriving Gosan passing through Santung peninsula or near South Korea.

• Korean Journal of Microbiology
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• v.21 no.3
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• pp.115-126
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• 1983

The results that the effect of 6 detergents on the structural changes and biochemical composition of bacterial membrane of Escherichia coli and Bacillus cereus are as follows ; 1. Population growth of the bacteria was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was decreased by sodium dodecyl sulfate and palmitoyl choline, in E.coli and was decreased by palmitoyl carnitine and palmitoyl choline at the low concentration, in B. cereus. 2. The electron micrograph showed that cell wall lysis or cell collapse were observed in the treatment of sodium dodecyl sulfate and palmitoyl choline, and also cell wall was condensed by triton X-100 and sodium deoxy cholate, in E.coli. And in B. cereus, endospore formation of the bacteria was stimulated by palmitoyl choline, and cell lysis or structural changes of the membrane were observed in the treatment of sodium dodecyl sulfate, sodium cholate, and triton X-100, respectively. 3. As to the effect of detergent on the biochemical composition of biomembrane, the content of carnitine, in E.coli, and B.cereus, the content of structural protein and phospholipid were decreased by treatment of sodium dodecyl sulfate and structural protein was denatured by palmitoyl choline. 4. The profile of membrane protein revealed that the bacterial membrane were composed of various proteins. By dint of this result, some of membrane proteins were solubilized or changed to small molecules by the treatment of sodium dodecyl sulfate and palmitoyl choline, in E.coli and membrane protein of the biomembrane by treatment of sodium dodecyl sulfate, sodium deoxy cholate, palmitoyl choline, and palmitoyl carnitine were confirmed to be different profile as compared with those of the control, in B. cereus. Therefore, it is suggested that sodium dfodecyl sulfate and palmitoyl choline soulbilized biomembranes or inhibited membrane transport and that palmitoyl carnitine and sodium deoxy cholate were used as an energy source or stimulating the membrane transport, in E.coli. And, it is suggested that all of detergents were inhibited biomembrane synthesis, expet saponin, in B.cereus.

• Journal of the Korean Chemical Society
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• v.54 no.5
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• pp.567-572
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• 2010

5,10-Methenyltetrahydrofolate synthetase from chicken liver was purified through 30-70% ammonium sulfate fractionation, Q Sepharose Fast Flow anion exchange and Source 15Phe hydrophobic interaction chromatography. Specific activities of cell extract, ammonium sulfate, Q Sepharose Fast Flow and Source 15Phe were 0.0085, 0.031, 0.80 and 1.27 U/mg, respectively. Purification fold activities of cell extract, ammonium sulfate, Q Sepharose Fast Flow and Source 15Phe were 1, 3.7, 94.1 and 149.4, respectively. HPLC gel permeation chromatography and SDS-polyacrylamide electrophoresis experiments indicated that the enzyme is a monomeric protein with a molecular weight of 22.8 kDa. Km for 5-methyl THF and Mg-ATP were $7.1\;{\mu}M$ and $63\;{\mu}M$, respectively. Optimum temperature and pH were $30^{\circ}C$ and 6.0, respectively. The data for metal ion specificity and stoichiometry showed that the maximum activity was obtained with a 1:l. ratio of $Mg^{2+}$. The ATP and Km values increased in the order of MgATP, MgCTP, MgUTP and MgGTP, and the maximum activities also decreased in the same order, indicating MgATP as the most efficient substrate. The enzyme was chemically modified only by tetranitrometane and 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide, indicating that tyrosine and carboxylate are present in the active site.