• Title/Summary/Keyword: Successful factors

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Optimization of a Virus-Induced Gene Silencing System with Soybean yellow common mosaic virus for Gene Function Studies in Soybeans

  • Kim, Kil Hyun;Lim, Seungmo;Kang, Yang Jae;Yoon, Min Young;Nam, Moon;Jun, Tae Hwan;Seo, Min-Jung;Baek, Seong-Bum;Lee, Jeom-Ho;Moon, Jung-Kyung;Lee, Suk-Ha;Lee, Su-Heon;Lim, Hyoun-Sub;Moon, Jae Sun;Park, Chang-Hwan
    • The Plant Pathology Journal
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    • v.32 no.2
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    • pp.112-122
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    • 2016
  • Virus-induced gene silencing (VIGS) is an effective tool for the study of soybean gene function. Successful VIGS depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. Recently, we developed a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative expression of GmPDS between non-silenced and silenced plants was measured by qRT-PCR. We found that gene silencing efficiency was highest at a photoperiod of 16/8 h (light/dark) at a growth temperature of approximately $27^{\circ}C$ following syringe infiltration to unrolled unifoliolate leaves in cotyledon stage with a final SYCMV:GmPDS optimal density $(OD)_{600}$ of 2.0. Using this optimized protocol, we achieved high efficiency of GmPDS-silencing in various soybean germplasms including cultivated and wild soybeans. We also confirmed that VIGS occurred in the entire plant, including the root, stem, leaves, and flowers, and could transmit GmPDS to other soybean germplasms via mechanical inoculation. This optimized protocol using a SYCMV-based VIGS system in the soybean should provide a fast and effective method to elucidate gene functions and for use in large-scale screening experiments.

Medico-Legal Consideration of Hemopneumothorax - Closing Claim Study- (${\cdot}$기흉과 관련된 의료법학적 문제에 대한 고찰 -종결된 사건을 중심으로-)

  • Bae, Hyu-Na;Cheon, Young-Jin
    • Journal of Chest Surgery
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    • v.39 no.2 s.259
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    • pp.117-126
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    • 2006
  • Background: The purpose of this study is to describe the characteristics of malpractice claims related to hemopneumothorax and to identify the causes and potential preventability of such claims. Material and Method: A retrospective study was performed by reviewing the records in the Lawnb website and Lx CD-rom: the records on closed malpractice claims involving hemopneumothorax were abstracted from the files available for analysis. The records were reviewed and were analysed to determine the etiology of hemopneumothorax, patient age, results of lawsuit and indemnity payment, underlying diseases, cause of death or complications, and the factors associated with a successful defense. Result: Seven closed claim involving hemopneumothorax were founded in the data for malpractice. Three claims were supreme court decision, one was a high court decision and three claims were district court decision. The most common cause of death was tension pneumothorax. Four of which resulted in indemnity payments. Conclusion: While malpractice claims involving hemopneumothorax were uncommon, they resulted in a high rate and amount of indemnity payments. Claims are more common in pediatric patients. In case of iatrogenic hemopneumothorax, post-procedural X-ray can improve patient outcome and is also associated with decreased indemnity risks. Informed consent is also important.

An Effects of Youth Founder's Entrepreneurship and Internal Locus of Control on Possibility of Start-up Success (청년창업자의 기업가정신과 내적 통제소재가 창업성공 가능성에 미치는 영향)

  • Kim, Soo-Hyun;Yoo, Bong-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.6
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    • pp.2677-2687
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    • 2013
  • Entrepreneurship of a founder and psychological characteristics are very important factors for the start-up success. To understand the importance like this, this study investigated effect relationships of the entrepreneurship and psychological characteristics on the start-up success. For this, 122 copies of the questionnaire were empirically analyzed for the youth entrepreneurs who entered the youth founder academy which is founded and managed by the Small and Medium Business Corporation. According to results of analysis, both the entrepreneurship consisting of proactivenss, risk taking and autonomy, and the internal locus of control, the psychological characteristics were found to have positive effects on the start-up success. Therefore, this study suggested theoretical and practical implications, as below, according to the results. Firstly, we must be aware of the importance of the entrepreneurship from the perspective of a successful establishment, and, prospective entrepreneurs, who are planning to the start-up, have to judge their entrepreneurship and, only then, make a decision. Secondly, we have to put our best efforts into developing more effective education on start-up, which deals with improving youth founder's entrepreneurship. Therefore, education institutions for start-up are needed in order to develop educational programs such as "Entrepreneurship Training Programs". Thirdly, we must recognize that the role of family is important because most entrepreneurship and psychological characteristics are formed in a person's childhood. Forth, the entrepreneur, themselves, must realize that they has to make an effort to improve their entrepreneurship. Lastly, youth founder's internal locus of control has to be enhanced. Thus, greater attention is required from both the entrepreneur themselves, and educational institutions for start-up.

Prediction of Uniaxial Compressive Strength of Rock using Shield TBM Machine Data and Machine Learning Technique (쉴드 TBM 기계 데이터 및 머신러닝 기법을 이용한 암석의 일축압축강도 예측)

  • Kim, Tae-Hwan;Ko, Tae Young;Park, Yang Soo;Kim, Taek Kon;Lee, Dae Hyuk
    • Tunnel and Underground Space
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    • v.30 no.3
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    • pp.214-225
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    • 2020
  • Uniaxial compressive strength (UCS) of rock is one of the important factors to determine the advance speed during shield TBM tunnel excavation. UCS can be obtained through the Geotechnical Data Report (GDR), and it is difficult to measure UCS for all tunneling alignment. Therefore, the purpose of this study is to predict UCS by utilizing TBM machine driving data and machine learning technique. Several machine learning techniques were compared to predict UCS, and it was confirmed the stacking model has the most successful prediction performance. TBM machine data and UCS used in the analysis were obtained from the excavation of rock strata with slurry shield TBMs. The data were divided into 8:2 for training and test and pre-processed including feature selection, scaling, and outlier removal. After completing the hyper-parameter tuning, the stacking model was evaluated with the root-mean-square error (RMSE) and the determination coefficient (R2), and it was found to be 5.556 and 0.943, respectively. Based on the results, the sacking models are considered useful in predicting rock strength with TBM excavation data.

Safening Mode of Action of 1, 8-Naphthalic Anhydride on Corn and Soybean Against Herbicide Bensulfuron and Imazaquin (제초제(除草劑) bensulfuron과 imazaquin에 대한 1, 8-naphthalic anhydride(NA)의 옥수수와 콩에 대한 약해경감작용기구(藥害輕減作用機構))

  • Hwang, I.T.;Choi, J.S.;Kim, J.S.;Cho, K.Y.;Chun, J.C.
    • Korean Journal of Weed Science
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    • v.14 no.1
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    • pp.8-15
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    • 1994
  • The mode of safening action and potency of the 1, 8-naphthalic anhydride(NA) were investigated in corn(Zea mays) and soybean(Glycine max) treated with herbicide bensulfuron[2-{{{{{(4,6-dimethoxy-2-pyrimidinyl)amino}carbonyl}amino}sulfonyl}methyl}benzoic acid] and imazaquin[2-{4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl} 3-quinolinonecarboxylic acid]. Seed dressing with 0.2%(w/w) NA showed successful protection in corn against injury from herbicide bensulfuron and imazaquin but not in soybean. Safening factors of NA against bensulfuron and imazaquin were 10.2 and 5.0, respectively, in corn, while they were both 1.3 in soybean. In vivo, Glutathione-S-transferase(GST) activity of NA-treated corn and soybean increased 1.8-and 1.3-fold, respectively, but the activity was not affected by the herbicides in vitro. Acetolactate synthase(ALS) levels of NA-treated corn was increased 1.3-fold, but not changed in soybean. Tolerance of ALS activity to the herbicides was slightly greater in ALS obtained from NA-treated corn than that from the untreated, whereas the difference was not found in soybean. A significant increase of ACCase due to NA occurred in corn, but not in soybean. The herbicides did not affect in vitro ACCase activity.

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Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

A study on quality of handicapped inpatients' service satisfaction at special dental clinic and their transformed perceptions toward to the dental treatment services (일부 복지관 장애인치과 내원 환자들의 서비스 만족도 및 진료 후 변화 인식도 연구)

  • Park, Soon-Ju;Choi, Seong-Woo;Park, Sun-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.6
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    • pp.1001-1014
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    • 2010
  • Objectives : The purpose of this study is about discovering the basic references to find the ways to vitalize handicapped dental clinics. Methods : The study was analyzed by the satisfactions of those patients who took the advantages of using the dental care at B-welfare center and also their transformed perceptions after the services are influenced. The analysis was the questionnaire consisting of 100 items and survey data. Results : 1. The satisfaction of the dental service for the disabled was respectively high which was the average of 4.49. 2. Their satisfactions of receiving kindness services by volunteers and employees were the highest in the entire research of successful handicapped dental services and it was 4.78 overall. 3. The satisfaction of using handicapped dental clinic has the higher range of female users than male's. In the mean time treatment details of the dental care and the satisfaction towards to the volunteers and faculty at the center show the statistical significance gap. 4. The oral care service after experiencing the dental clinic for handicapped relived their discomforts of using the regular dental clinic which shows their highest satisfaction as it is the point of 4.75. 5. the change perception after dental treatment for handicapped has the higher range of females than men's and solving the problems of mouth reference and discomfort of using regular clinics show the statistical significance gap. 6. In the change perception after having dental treatment for handicapped the thought of the possibility of periodical dental care shows the highest perception when the number of visiting is usually shorter and it shows the statistical significance. Conclusions : According to the satisfaction of those inpatients who use free dental care services that belong to dental clinics for handicapped in a part of Seoul welfare centers human services were appeared as the most important factor due to their advantages of taking services from volunteers and staff members. On the other hand to enhance the medical treatment information and environment which showed the weakest factors each inpatient should be specifically specialized for their needs and also further study on plans which enhance their perceptions toward to a better quality of oral-related life is required after using dental treatment service.

The Clinical Evaluation of The Reconstruction of Radial Forearm Free Flap in the Head and Neck Cancer Surgery (두경부 악성 종양 절제술후 요골 전완 유리피판을 이용한 재건술의 평가)

  • Kim Hyun-Jik;Lim Young-Chang;Song Mee-Hyun;Lee Won-Jae;Choi Eun-Chang
    • Korean Journal of Head & Neck Oncology
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    • v.19 no.2
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    • pp.164-169
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    • 2003
  • Background and Objectives: The reconstruction is very important in Head and neck cancer surgery to repair the defect created by resection of tumors, to enable successful wound healing, to restore function and to provide acceptable cosmesis. The radial forearm free flap has been the most useful reconstructive flap because it provides a moderate amount of thin, pliable, relatively hairless skin and comparatively simple to do with minimal morbidity. The aims of this study is to estimate the outcome of the reconstruction with radial forearm free flap with the several factors in 140 head and neck cancer cases in our hospital for last 10 years. Materials and Methods: Retrospective review of the records of 140 patients underwent resection of the head and neck tumors and reconstruction with a radial forearm free flap from 1993 to 2003. The age, sex of the patients, Primary site, the complication of donor and recipient site, flap survival rate, median time to start diet, patient subjective symtoms about swallowing and articulating and the fact of revision reconstructive surgery were analyzed. Results: In primary pathologic site, 56 cases were oral cavity cancers, 44 cases, oropharyngeal cancers and 22 cases, hypopharyngeal cancers. Flap survival rate was 93.6% (13 leases). On donor site, wound dehiscence, hematoma, sensory change and infection were noted and on recipient site, most common complication were fistula and wound dehiscence. The complication rate of recipient's site was 19.1 % and donor site, 3.5%. In 118 cases (84.3%), the patients could take all kinds of food. Swallowing difficulty were noted in 22 cases 05.7%). In 5 cases, there was articulation difficulty but most of patients except patients having total laryngectomy (18 cases) couldn't have any difficulty in articulation and speaking. Conclusion: We conclude that the radial forearm free flap is the most appropriate reconstructive material for treating the defect in head and neck reconstruction.

Problem Solver's Responses According to the Sentence Structures of Mathematical Word Problems (수학 문장제의 문장 구조에 따른 초등학생의 문제해결 반응 비교 분석)

  • Kang, Wha-Na;Paik, Suck-Yoon
    • Journal of Educational Research in Mathematics
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    • v.19 no.1
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    • pp.63-80
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    • 2009
  • This paper has a purpose to find out the important points about linguistic factors suited to the assessment purpose and mathematics teaching/learning that a word-problem sentence has to possess. We also examine the degree of understanding of sentence and the perceptive/emotional reactions of students toward two different kinds of word-problem sentences that have same mathematical contents, but different linguistic structures. The objects of this thesis are 124 students from the third to sixth grade in an elementary school. We execute assessment of simple-sentence-word-problem and complex-sentence-word-problem that have same mathematical contexts, but different linguistic structures. Then we have compared and examined their own process of solving the two types word-problems and we make up questionnaire and have an interview with them. The conclusions are as followings: First, simple-sentence-word-problem is more successful to suggest an information for solving a problem than complex one. Second, it is hard to find the strategy for solving a problem in complex-sentence-word-problem than simple one. Third, students think that suggested information and mathematical knowledge are different according to the linguistic structure in the process of perceiving the information after reading a word-problem. Fourth, in spite of same sentence type, the negative mental reaction is showed greatly to complex-sentence-word-problem even before solving a problem.

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Rhabdomyosarcoma Involving Maxillary Sinus and Orbit (상악동과 안와를 침범한 횡문근육종 1례)

  • Oh Yong-Suk;Kang Jin-Hyoung;Han Ji-Youn;Hong Young-Sun;Kim Hoon-Kyo;Lee Kyung-Shik;Kim Dong-Jip;Kim Min-Sik;Cho Seung-Ho;Suh Byung-Do;Yoon Sei-Chul
    • Korean Journal of Head & Neck Oncology
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    • v.10 no.2
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    • pp.218-224
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    • 1994
  • Soft tissue sarcoma of the head and neck is not frequent neoplasm, accounting for less than 1% of all malignant neoplasm in the region. The histological varieties include osteogenic sarcoma, malignant fibrous histiocytoma, rhabdomyosarcoma, fibrosarcoma, tenosynovial sarcoma, angiosarcoma and chondrosarcoma. Rhabdomyosarcomas of the head and neck usually occur in children under the age of 10 years (over 70%) and rarely develop in adults over the age of 20 years. The prevalent sites of involvement include the orbit, nasal cavity, external ear, paranasal sinus and soft tissue of mouth and the primary location of tumor is considered to be one of the important prognostic factors. Before the 1960s, when surgical resection was the only method of treatment, the 5-year survival rate was less than 20%, but recently it has been greatly improved by the multimodality treatment, combining surgery with chemotherapy and radiation therapy. Here we treated a rhabdomyosarcoma woman with three cycles of high dose chemotherapy followed by radiation therapy. After the, completion of preoperative treatments, successful result of more than partial response was achieved. Three months later total maxillectomy and radical neck dissection was performed. There was no evidence of tumor infiltration in the resected tumor and regional lymphnodes but metastasized tumor cells in cervical lymphnodes were detected. Tumor cell infiltration was also found on the bone marrow biopsy to evaluate the pancytopenia which occurred during postoperative recovery. Two months later she died of secondary bone marrow failure. We think that this multimodality treatment combining pre-operative chemotherapy, radiotherapy and surgery might play an important role in curative resection and eyeball preservation in patients with rhabdomyosarcoma involving the eyeball.

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