• The Korean Journal of Zoology
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• v.32 no.4
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• pp.412-419
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• 1989

Monoclonal antibodies (MAbs) reacting with the plasmalemma of Amoeba proteus were produced. Specificity of the 3 MAbs was determined by transfer blotting of the SDS polvacryfamide gel. AMS antibody reacted with the mucopolysaccharide bands of the spacer gel, 220 KD and 50 KD proteins of the resolving gel. The maior glycoprotein bands (175 KD, 165 KD) and 50 KD protein of the plasmalemma were recognized by AUG antibody. A third, AMP antibody reacted with the 50 KD protein only. In immunofluorescence microscopy of the enzyme treated cells, the antigens of these MAbs were sensitive to proteases, but not sensitive to neuraminidase. In the assay of cell to substratum attachment after binding with the antibody, AMG and AMP antibodies exerted no effect, but AMS hindered the attachment and cell spreading. Thus the effective components of the plasmalemma in cell to substratum attachment appear to be the mucopolysaccharides and 220 KD protein. The membranes of latex particle infested phagosomes did not show any distinction from the plasmalemma in fluorescence microscopy. Phagosome membranes of amoebae appear to be derived from the plasma membrane without selection in terms of the antigen composition. Amoeba Proteus의 세포막과 반응하는 단세포군 항체를 생산하였다. SDS polyacrylamide gel을 transfer blotting하여 이들 항체의 반응 특이성을 조사해 본 결과 AMS 단항체는 PAS로 염색되는 spacer gel의 mucopolysaccharide 린드, resolving gel의 220 KD 및 50 KD 단백질과 반응하였으며, 세포막의 주요 당단백질인 175 KD 및 165 KD 빈드와 50 KD 단백질은 AMG 단항체에 의해서 인지되었다. 그리고 AMP단항체는 공통인 50 KD 단백질과 특이하게 반응하였다. 효소처리한 아메바의 면역형광칠미경적 조사에서 이들 항체에 대한 항원분자들은 모두 단백질분해효소에 민감하였으며 neuraminidase에 대해서는 변화가 없었다. 이들 항체를 결합시킨 아메바의 용기표면 부착 가능성을 분석한 결과 AMP 및 AMG 단항체는 아무런 영향을 미치지 못하였으며 AMS 단항체는 세포의 용기표면 부착 및 세포의 펴짐을 저해하였다. 따라서 아메바의 용기표면 부착은 mucopolysaccharide 및 220 KD 단백질에 의해서 매게되는 것으로 나타났다. 그리고 latex particle을 담고 있는 식포막은 면역형광형미경적 조사에서 세포막과 차이가 없었다. 따라서 겐포막은 항원 조성에 있어서 비 선택적으로 세포막에서 유도되는 것으로 나타났다.

• The Journal of Korean Academy of Prosthodontics
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• v.45 no.3
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• pp.375-381
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• 2007

Statement of problem. Prior to determining an optimal width of micropatterned grooves provided on titanium substrata, we have done a pilot study using surface topographies in combined microm and submicrom levels. Purpose. The purpose of this study was twofold 1) to assess the proliferation and 2) to analyze the expression of genes encoding the intracellular signaling proteins involved in cell-substratum adhesions and adhesion-dependent G1 phase cell cycle progression of human gingival fibroblasts plated on smooth and microgrooved/acid-etched titanium substrata. Material and methods. Three groups of titanium discs as NE0 (smooth Ti substrata), E15 (Ti substrata with microgrooves of $15{\mu}m$ of spacing and $3.5{\mu}m$ in depth and with further acidetching), and E30 (Ti substrata with microgrooves of $30{\mu}m$ spacing and $3.5{\mu}m$ in depth and with further acid-etching) served as the human gingival fibroblasts' substrata. Viability and proliferation of fibroblasts were determined using an XTT assay. Gene expressions of fibronectin, ${\alpha}5$ integrin, CDK4, and $p27^{kip}$ were analyzed in RT-PCR. Cell-substratum interactions were analyzed in SEM. Results. From the XTT assay at 24 h incubation, the mean optical density (OD) value of E15 was significantly greater than the values of E30 and NE0. At 48 and 96 h however, the mean OD values of E30 were significantly greater than the values of E15 and NE0. No differences in the expression of PCR transcripts at 96 h incubations were noted between groups, whereas at 48 h, an unexpected increase in the expression of all the transcripts were noted in E15 compared with other two groups. Fibroblasts were observed to orient and adhere inside the microgrooves. Conclusion. Micropatterned grooves and acid-etching on Ti substrata alter viability and gene expression of adhered human gingival fibroblasts.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.457-464
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• 1995

Hela cells, a transformed human epithelial cell line, attach to various substrata but subsequent spreading is specific to collagen or gelatin. The spreading is initiated by the activation of phospholipase $A_2$ (PLA$_2$) which produces arachidonic acid (AA) as a consequence of cell surface collagen receptor clustering. This study examines the mechanism of PLA$_2$activation and which phospholipids are hydrolyzed by PIA$_2$ to release AA in response to Hela cell adhesion to a gelatin substratum. The levels of phosphatidyicholine decreases, among various phospholipids, during attachment and spreading of Hela cells. Lysophosphatidyicholine Is the only lysophospholipids formed during ileLa cell adhesion indicating that clustered collagen receptors activate PLA$_2$to hydrolyze posphatidylcholine to AA and lysophosphatidylcholine. Among various molecular entitles which are known to regulate PLA$_2$ activation, we have previously shown that PLA2 activation is not mediated by either changes in $Ca_2$+ levels, alkalinization of cytoplasmic p11, or activation of protein hinase C. It is also likely that PIA2 activation is not mediated by either pertussis or cholera toxinsensitive G proteins as those toxins do not affect both AA release and cell spreading.

• Journal of Wetlands Research
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• v.23 no.4
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• pp.317-326
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• 2021

The present study was carried out with a view to understand the distribution and ecology of Clihton retropictus (Martens, 1879), a class II endangered wildlife designated and protected by the Ministry of Environment, Republic of Korea, and to suggest the effective conservation and management measures for this species and its habit. First, the distribution of Clihton retropictus was investigated nationwide, and environmental factors such as salinity and substratum were analyzed in consideration of the ecological characteristics of this species. In addition, the individual size, density, and movement of Clihton retropictus per each district surveyed were analyzed. Due to its nature of inhabiting in the brackish water zone, the analysis showed that the salinity of this species was 0.7 ~ 16.6‰ at low tide and 1.0 ~ 17.3% at high tide. It was learned from the analysis of substratum that its composition in each river under investigation was slightly different, but most of substratum had a higher composition ratio than gravel (pebble, 16 ~ 64 mm). On the other hand, the size of each individual was checked by measuring the length of its diameter, and there was almost no change in the individual when the inhabited range of Clihton retropictus was short. However, there was tendency that the longer the habitat range, the bigger the individual size. From surveying the population density by season, it was made clear that, during the summer (July to August) and fall (September to October), the density was relatively higher while the number of individuals decreased significantly during winter (November to December) when the water temperature was lowered. In an effort to effectively establish conservation and management measures, the moving distance was measured, and it became known that, during the surveys in summer and fall when the individuals were active, the individuals moved in more distances than during the winter survey. The results of this study are expected to be utilized as basic data for establishing effective management plans such as creation of habitats for Clihton retropictus, its migration projects and selection of migration destinations which get unavoidable due to development projects.

• Fisheries and Aquatic Sciences
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• v.4 no.2
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• pp.93-97
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• 2001

Morphological features of the two Korean Acrosorium species, A. flabellatum Yamada and A. venulosum (Zanardini) Kylin (Delesseriaceae, Rhodophyta) are described based on specimens collected from several localities along the coasts of Korea. Acrosorium flabellatum is easily distinguished from other Acrosorium species in lacking rhizoids in branchlets for attachment to substratum. The flabellate branching is also characteristic for this species. By contrast, Acrosorium venulosum is distinct from others in having the unique hooked branchlets. Taxonomic features found in the Korean Acrosorium species are compared, and a key to the species is provided based on this and previous data.

• Animal cells and systems
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• v.8 no.3
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• pp.139-143
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• 2004

Two new species of the genus Psammocinia (Dictyoceratida, Irciniidae), P. conulosa n. sp. and P. ulleungensis n. sp., are described from Namhaedo Island and Ulleungdo Island, Korea. Psammocinia conulosa n. sp. seems to be close to both P. amodes Cook and Bergquist, 1998 and P. hawere Cook and Bergquist, 1996 on the basis of the skeletal structure. However, these three species are clearly separated by the following differences: Shape of P. amodes is spatulate and thin, with a broad blade narrowing to a semi-cylindrical stalk; P. hawere forms cups with a shallow excavated bowl, and attached to the substratum by a narrow base. The whole surface of the new species is very finely conulose. Psammocinia ulleungensis n. sp. is similar to P. gageoensis Sim and Lee, 2001 in shape, but this species is easily distinguished from P. gageoensis by the simple skeletal structure.

• Journal of Environmental Health Sciences
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• v.27 no.1
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• pp.100-105
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• 2001

Microstructural examinations were performed on the anaerobic biofilm from reactor filled with PE support media. Optical microscope, SEM and fluorescent microscope were used for qualitative and morphological studies on the attached microorganism under anaerobic condition. Microorganisms were attached in crevices where protection from shear forces of surfaces where easy to contact with support media surface. A hypothesis for biofilm accumulation occurs on a surface such as polymer support media is presented schematically : 1st step ; cell-support media attachment, 2nd step ; cell-support media attachment and cell-cell attachment, 3rd step ; attached biofilm from neighboring crevices joins together and growing, 4th step ; mature and irregualar biofilm was formed. In SEM photographs, shape and structures of biofilm were observed, but microorganism species and methanogens were not identified. A large number of methanogenic bacteria were identified on the surface of PE substratum by fluorescence under 480nm of radiation and it was estimated that methanogenic bacteria was related to initial attachment of bacteria under anaerobic condition.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.502-506
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• 2005

2003년 11월과 2004년 4월의 2회에 걸쳐 상용조류발전소 건설 예정지인 울돌목에서 발전소 건설로 인해 야기될 주변생태환경의 변화를 파악하기 위해 4개 지점의 주변 암반조간대에 형성된 기존의 부착생물상 조사가 수행됐다. 울돌목 주변의 암반조간대에 형성된 부착생물상은 서식 형태에 따라 크게 대형해조류(Macroalgae)를 포함한 고착생물, 소 이동성 부착동물, 이동성 소형 무척추동물 등으로 구분된다 부착생물상은 서식양상에 있어 각각의 정점별 특이성이 관찰되었으며, 이는 각기 다른 비생물학적 요인에 따라 지역 특유의 서식환경이 조성되어 있음을 시사하는 간접적인 결과로 간주된다. 이같은 지역 간 생물상 차이는 조위에 따른 수직분포 분석결과 주로 중부조간대를 중심으로 형성된 생물상에 따라 대별되고 있음이 발견되었다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.137-144
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• 1994

Spherical multicellular aggregates of adult rat hepatocytes (spheroid) which have tissue like structure, were formed and immobilized in the pores of polyurethane foam (PUF) which was used as a culture substratum. These hepatocyte/spheroids, about 100 $\mu\textrm{m}$ in diameter, have maintained higher differentiated functions than those of hepatocyte/monolayer for about 3 weeks in serum-free medium. Then, we designed a prototype module of an artificial liver support system using a PUF/spheroid packed-bed, in which hepatocyte/spheroids were immobilized at high density. The urea synthesis activity of the artificial liver was maintained at least 10 days in 100% rat blood plasma. We start examining the performance of hybrid artificial liver in an ex vivo extracorporeal experiment with an acute hepatic failure rat.

• Magazine of the Korea Concrete Institute
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• v.8 no.1
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• pp.121-129
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• 1996

본 연구에서는 대기 습도변화에 의한 콘크리트 보수체(기층 콘크리트/보수 모르터)의 파괴현상을 조사하기 휘해서 보수체내의 비정상적인 습도분포를 계산하였다. 계산된 습도분포에 의해서 기층과 보수층 경계면 부위의 습도차이가 보수층 두께(0.5~2.5cm)와 보수작업전 기층 콘크리트 표면의 습윤처리시간(0~72hr)을 주요 변수로 하여 조사되었고, 이는 주로 시멘트 모르터로 보수된 접촉면이 없는 보수체에서 수행되었다. 계산 및 조사결과 보수층 두께가 감소하고 습윤 처리시간이 증가할수록 경계면 부위의 습도차이는 감소하는 경향을 보였고, 특히 보수후 습도차이가 음수값이 될 때의 시간을 하나의 수식으로 표시하였다.