• KSBB Journal
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• v.16 no.5
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• pp.466-473
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• 2001

A bacterium was isolated from soils in Suwon, Korea for the purpose of H$_2$S removal using a biofilter system. The isolate was gram-negative, rod-shaped, catalase-positive, motile, and the isolated bacterium showed a positve in utilizing energy sources including citrate, mannitol, sucrose, fructors, and trehalsoe. Based on its biochemical characteristics it was identified as Burkholderia(Pseudomonas) cepacia. The growth rate of the bacterium in thiosulfate medium with yeast extract was 0.15 hr$\^$-1/ and generation time was 4.6 hr. The cell productivity was 8.05 mg/L$.$h and the isolate grew logarithmically up to 12 hr. The maximum rate of sulfur oxidation was 0.18 g-S/L$.$h. The optimum pH and temperature for the growth of the bacterium were 7.0 and 30$\^{C}$, respectively. The pH range for the growth of B. cepacia was 5.0-8.0. The oxidation rate of thiosulfate was lowered by a substrate thiosulfate when the concentration was higher than 0.12 M. both growth rate and sulfur oxidation rate of Burkholderia(Pseudomonas) cepacia was enhanced about 1.5 times with the addition of 0.2% yeast extract. The removal of hydrogen sulfide was investigated by immobilized B. cepacia with Ca-alginate. The maximum rate removal for H$_2$S was 6.25 g$.$㎤ $.$h$\^$-1/ when 12 L/h of flow rate was supplied. From this study suggest the immobilized B. cepacia could have a potential for H$_2$S removal.

• Journal of Korean Society of Steel Construction
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• v.28 no.5
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• pp.373-382
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• 2016

Painting or thermally sprayed metal coating is often used in corrosion protection of steel structures. In recently, duplex coating system which combines thermally sprayed metals with paint is selected as a new generic type of coatings on steel structures under the highly corrosive environments. In this study, the structural steel specimens were surface treated, thermally sprayed with zinc, zinc-15%aluminum alloy, aluminum and aluminum-5%magnesium alloy, and finally sealing or painted with acrylic urethane. And as a reference specimens, steel specimens were painted with acrylic urethane after surface treatment. Circular defects with 1.0, 3.0 and 5.0 mm in diameters and line defect with 2.0 mm width, which reach the steel substrate were created on all specimens. The specimens were exposed into an environmental testing chamber controlled by the ISO 20340, which is a laboratory cyclic accelerated exposure test condition of spraying/UV/low temperature, for up to 175 days. Based on the corrosion tests, corrosion deterioration from the initial defects were evaluated and weathering performance of the specimens are compared.

• Polymer(Korea)
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• v.29 no.2
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• pp.146-150
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• 2005

A nano-stereolithouaphy (NSL) apparatus has been developed for fabrication of microstructures with the resolution of 150 nanometers. In the NSL process, a complicated 3D structure can be fabricated by building layer by layer, so it does not require any sacrificial layer or any supporting structure. A laminated layer was fabricated by means of solidifying liquid-state monomers using two-photon absorption (TPA) which was induced by a femtosecond laser. When the fabrication of a 3D laminated structure was finished, unsolidified liquid-stage resins were removed to develop the fabricated structure by dropping several droplets of solvent, then the polymerized structure was only left on the glass substrate. A microstructure is fabricated by vector scanning method to save the fabrication time. The shell thickness of a structure is very thin within 200 nm, when it is fabricated by a single contour scanning (SCS) path. So, a fabricated structure can be deformed easily in the developing process. In this work, a double contour scanning (DCS) method was proposed to reinforce the strength of a shell typed structure, and a microcup was fabricated to show the usefulness of the developed NSL system and the DCS method.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.351-357
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• 1997

The cyclodextrin glycosyltransferase(EC 3.2.1.19) from Bacillus firmus was purified by precipitating with ammonium sulfate followed by, DEAE-Sephadex A-50 column chromatography and Sephadex G-100 column chromatography. In this way, we were able to obtain the single band protein on SDS-PAGE with a yield of 12%, whose purity was 49 fold. The purified CGTase was identified as a protein having molecular weight of approximately 80,000 dalton and isoelectric point of 9.6. The optimum pH and temperature for the enzyme activity were 8.0 and $65^{\circ}C$, respectively. The enzyme was stable at between pH 5.5 and 9.0 and up to $50^{\circ}C$. After 24hr of enzyme reaction using soluble starch as substrate, the ratio of ${\alpha}-$, ${\beta}-$ and ${\gamma}-cyclodextrin$ production was 0.01 : 2.90 : 1.00, respectively. And this CGTase pro-duced mainly ${\beta}-$ and ${\gamma}-cyclodextrin$.

• Journal of Korea Water Resources Association
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• v.38 no.7 s.156
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• pp.545-554
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• 2005

The detailed interdisciplinary surveys were conducted on the pattern of habitat use of dominant fishes during the spawning and adult stage. The hydraulic parameters of the depth and velocity, discharge, substrate cover streams, and distribution of fish in the Yeonggang, WiCheon, HoeCheon, GeochangwiCheon, CheongdoCheon, DanjangCheon (the Nakdong River Basin) were measured. The Habitat Suitability Criteria was developed for the two fish species (Zacco Platypus and Zacco Temmincki) and life stages(spawning and adult), habitat conditions (depth, velocity and covet). The Physical Habitat Simulation of the Instream Flow Incremental Methodology was applied to calculate for optimal flow and the ecological flow recommendation was proposed by choosing the largest one in the optimal flow. The ecological flow recommendation was $5.0\;m^3/s{\sim}10.0\;m^3/s$ (e.g., $6.5\;m^3/s$ in the NaeseongCheon). Also, the ecological flow recommendations were compared with the existing ecological flow and flow duration analysis.

• Journal of the Korean Vacuum Society
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• v.21 no.3
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• pp.178-184
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• 2012

Carbon coils could be synthesized on nickel catalyst layer-deposited silicon oxide substrate using $C_2H_2$ and $H_2$ as source gases and $SF_6$ as an additive gas under thermal chemical vapor deposition system. The characteristics (formation density and morphology) of as-grown carbon coils were investigated as functions of additive gas flow rate and the cycling on/off modulation of $C_2H_2/SF_6$ flows. Even in the lowest $SF_6$ flow rate (5 sccm) in this work, the cycling on/off modulation injection of $SF_6$ flow for 2 minutes could give rise to the formation of nanosized carbon coils, whereas the continuous injection of $SF_6$ flow for 5 minutes could not give rise to the carbon coils formation. With increasing $SF_6$ flow rates from 5 to 30 sccm, the cycling on/off modulation injection of $SF_6$ flow confines the geometry for the carbon coils to the nanosized ones. Fluorine's role of $SF_6$ during the reaction was regarded as the main cause for the confinement of carbon coils geometries to the nano-sized ones.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.176-177
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• 2013

Surface plasmon polaritons (SPPs) have attracted the attention of scientists and engineers involved in a wide area of research, microscopy, diagnostics and sensing. SPPs are waves that propagate along the surface of a conductor, usually metals. These are essentially light waves that are trapped on the surface because of their interaction with the free electrons of conductor. In this interaction, the free electrons respond collectively by oscillating in resonance with the light wave. The resonant interaction between the surface charge oscillation and the electromagnetic field of the light constitutes the SPPs and gives rise to its unique properties. In this papers, we studied theoretical and experimental extraordinary transmittance (T) and reflectance (R) of 2 dimensional metal hole array (2D-MHA) on GaAs in consideration of the diffraction orders. The 2d-MHAs was fabricated using ultra-violet photolithography, electron-beam evaporation and standard lift-off process with pitches ranging from 1.8 to $3.2{\mu}m$ and diameter of half of pitch, and was deposited 5-nm thick layer of titanium (Ti) as an adhesion layer and 50-nm thick layer of gold (Au) on the semiinsulating GaAs substrate. We employed both the commercial software (CST Microwave Studio: Computer Simulation Technology GmbH, Darmstadt, Germany) based on a finite integration technique (FIT) and a rigorous coupled wave analysis (RCWA) to calculate transmittance and reflectance. The transmittance was measured at a normal incident, and the reflectance was measured at variable incident angle of range between $30^{\circ}{\sim}80^{\circ}$ with a Nicolet Fourier transmission infrared (FTIR) spectrometer with a KBr beam splitter and a MCT detector. For MHAs of pitch (P), the peaks ${\lambda}$ max in the normal incidence transmittance spectra can be indentified approximately from SP dispersion relation, that is frequency-dependent SP wave vector (ksp). Shown in Fig. 1 is the transmission of P=2.2 um sample at normal incidence. We attribute the observation to be a result of FTIR system may be able to collect the transmitted light with higher diffraction order than 0th order. This is confirmed by calculations: for the MHAs, diffraction efficiency in (0, 0) diffracted orders is lower than in the (${\pm}x$, ${\pm}y$) diffracted orders. To further investigate the result, we calculated the angular dependent transmission of P=2.2 um sample (Fig. 2). The incident angle varies from 30o to 70o with a 10o increment. We also found the splitting character on reflectance measurement. The splitting effect is considered a results of SPPs assisted diffraction process by oblique incidence.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.3
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• pp.167-173
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• 2011

The Effects of external resistance on electrical properties such as current density, power density and coulombic efficiency were investigated in two-chamber type MFCs using a ferricyanide as reducing agent. A stable electricity was produced when a constant time elapsed after innoculation of mixed cultures into the anode compartment; voltages from 0.13 to 0.16 V was measured at $50{\Omega}$ of external resistance. When the external resistance was increased, the current density decreased and the power density rapidly increased and then slowly decreased. Big variation of electrical properties was observed in high-current density region due to the concentration loss related with substrate consumption in repeated experiments changing the external resistance. The maximum power density ($175.8mW/m^2$) and coulombic efficiency (46.1%) were obtained at $100{\Omega}$ of the external resistance which is nearest with the internal resistance ($134{\Omega}$) of MFC system.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.58-62
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• 2006

Rapid assay of enzyme is a primary requirement for successful application of directed evolution technology. Halo generation on a turbid plate would be a method of choice for high throughput screening of enzymes in this context. Here we report a new approach to prepare turbid plates, by controlling the crystallization of tyrosine to form needle-like particles. In the presence of tyrosine phenol-lyase (TPL), the needle-like tyrosine crystals were converted to soluble phenol rapidly than the usual rectangular tyrosine crystals. When an error-prone PCR library of Citrobacter freundii TPL was spread on the turbid plate, approximately 10% of the colonies displayed recognizable halos after 24 hours of incubation at $37^{\circ}C$. Representative positives from the turbid plates were transferred to LB-medium in 96-wellplates, cultivated overnight, and assayed for the enzyme activity with L-tyrosine as the substrate. The assay results were approximated to be proportional to the halo size on turbid plates, suggesting the screening system is directly applicable to the directed evolution of TPL. Actually, two best mutants on the turbid plates were identified to be $2{\sim}2.5$ and 1.5-fold improved in the activity.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.267-273
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• 1982

Among 12 kinds of ginsenosides in ginseng saponin, ginsenoside-Rb$_1$was contained the most abundantly. But ginsenoside-Rd which is similar to ginsenoside-Rb$_1$in structure, was known to be superior to ginsenoside-Rb$_1$pharmaceutically. In order to convert ginsenoside-Rb$_1$into ginsenoside-Rd by microbial enzyme treatment, a Rhizopus sp. was selected among various strais of molds found in rotten ginseng roots. Enzyme was prepared from the extract of wheat bran koji culture by ammonium sulfate precipitation (1.0 sat'd) and succeeding ammonium sulfate fractionation method (0.6-0.9 sat'd). For the purpose of use as substrate, saponins were purified by the several purification steps from alcohol extract of red ginseng roots. We obtained the total saponin which was composed of 36.5% of ginsenoside Rb$_1$, 12.2% of ginsenoside-Rd and other ginsenosides. For increase of ginsenoside-Rb$_1$ component ratio, we also obtained further purified ginsenoside-Rb group saponin containing 54.5% of ginsenoside-Rb$_1$, 1.1% of ginsenoside- Rd and other ginsenosides from purified the total saponin. In the enzymatic reaction system including the total saponin or the ginsenoside-Rb group saponin, we confirmed the specific conversion of ginsenoside-Rb$_1$to ginsenoside-Rd proportionally and no change of any other ginsenoside patterns by thin layer chromatography and high performance liquid chromatography.