• Journal of Bio-Environment Control
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• v.16 no.2
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• pp.108-114
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• 2007

This study was conducted to clarify the effects of the different mixing ratios of substrate mixtures based on peat moss and the concentration of nutrient solution on the growth of tomato (Lycopersicon esculentum Mill.) seedlings. Substrates such as peat moss, rice hull, carbonized rice hull, decomposed sawdust, perlite and granular rock wool were mixed and used. The concentration of nutrient solution were adjusted to EC $0.5{\sim}1.5mS/cm$. The volumetric moisture contents became higher as peat moss mixed were much more. Total porosities in all substrate mixtures were over 80%, and pH in substrate mixtures became lower as the volume of peat moss mixed higher. Mixing ratios of substrates suitable for the production of tomato seedlings with the higher quality were peat moss:rice hull:carbonized rice hull:decomposed sawdust:perlite=25:10:25:20:20(v/v). The plant growth was not significant among the different substrate mixtures. However, plant growth such as plant height, leaf area, and total dry weight became significantly increased as EC increasing.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.2
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• pp.91-95
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• 2009

To understand the effect of attachment substrate on the growth of benthic microalgae, we experimentally investigated the growth of benthic microalgae Nitzschia sp. (Jinhae Bay strain) with additions of glass beads in different sizes. The glass beads used in this study are 0.09-0.15 mm (G1), 0.25-0.50 mm (G2), 0.75-1.00 mm (G3) and 1.25-1.65 mm (G4). No addition of glass beads used as controls. Highest specific growth rate (0.37/day) and maximum cell density ($9,232{\pm}840$ cells/mL) of Nitzschia sp. showed at the smallest glass beads (G1), and the specific growth rate and maximum cell density were decreasing with increasing size of glass beads (specific growth rate and maximum cell density of G4 was 0.24/day and $6,397{\pm}524$ cells/mL, respectively). Moreover, specific growth rate of the control experiment (0.23/day) was significantly lower than their of G1 to G3 experiment. The results indicated that the attachment substrate for benthic microalgae as Nitzschia sp. is important factor which affecting the growth rate as well as cell density. Therefore, the physiological experiment of benthic microalgae seems to be necessary to preliminary experiment, which is addition or not of the attachment suitable substrate and the grain size for the target species of benthic microalgae.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.150-154
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• 2016

To determine a favorable substrate formulation for Grifola frondosa, physicochemical conditions, culture properties, and yields according to various substrate formulations were investigated. Based on these analyses, T4 (80:5:15 ratio of oak sawdust to dried bean-curd refuse to corn husk) resulted in a shorter cultivation period and higher yields (weight of fresh mushrooms harvested at maturity) than those of other treatments. The physicochemical properties of T4 were pH 5.4, 2.4% crude fat contents, 54 C/N ratio, 74.3% porosity, and 0.26 g/cm3 bulk density. These results emphasize the importance of optimal substrate development on the production efficiency of G. frondosa mushrooms and have implications for commercial applications.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.290-295
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• 2019

This study was carried out to select the optimal condition for prothallus propagation and sporophyte formation of Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike and to provide basic data for mass production system. In the propagation, 300 mg of prothallus were inoculated in different kinds of medium [1/4, 1/2, Murashige and Skoog (MS), Knop], sucrose (0, 1, 2, 3, 4%), and cultured for 8 weeks. Sporophyte formation studies were carried out by inoculating blended prothallus into artificial soils. The soils were mixed with horticultural substrate, peatmoss, perlite, and decomposed granite at different ratios or only with the horticultural substrate. Then the prothallus was cultivated for 12 weeks to figure out the formation and growth of the sporophytes. The growth and development of prothalli were excellent on MS medium. According to medium components, prothallus growth was favorable in all treatments except 0% sucrose treatment and the highest in 2% sucrose. In the experiment of soil mixtures, sporophyte formation was the highest in the horticultural substrate:perlite 2:1 (v:v) and horticultural substrate:decomposed granite 2:1 (v:v) treatment, and the overall growth was good in the horticultural substrate:decomposed granite 2:1 (v:v) mixed soil.

• Journal of Life Science
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• v.18 no.7
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• pp.980-985
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• 2008

This study was carried out to develope an alcoholic drink by fermentation of onion extract using Saccharomyces cerevisiae. The optimal conditions for ethanol production were obtained by standing culture at $25^{\circ}C$ for 5 days with 5% inoculum volume. At the results by flask culture, the growth curve of used S. cerevisiae reached to the stantionary phase at 48 hr and the death phase at 90 hr, whereas ethanol production reached maximum at 114 hr. Under the above conditions, a large scale production was carried out. A standing culture in 5 l fermenter showed the similar results to its flask culture, but progressed 24 hr rapidly more than that of the flask culture. A fed-batch culture was performed by addition of the onionic medium supplemented with 10% (v/v) sucrose after 72 hr from the fermenting start. The fed-batch culture could prevent S. cerevisiae from entering into the death phase and maintain constant level of alcohol production. A continuous culture was able to carry out by adding per every 24 hr the onionic medium supplemented with 10% (v/v) sucrose after 72 hr from the fermenting start. Although S. cerevisiae used showed a little decreased growth, alcohol production maintained roughly the constant level at the maximum yield. To enhance the quality of this alcoholic drink, $2-O-{\alpha}-D-glucopyranosyl$ L-ascorbic acid (AA-2G) was supplemented into the onion extract of the substrate for fermentation. As resulted at this study, this alcoholic drink containing AA-2G should be used as a functional fermented alcohol drink strengthened with vitamin C.

• Journal of Bio-Environment Control
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• v.5 no.2
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• pp.131-137
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• 1996

This analysis has been conducted to get some basic data of the composition of culture solution mostly in inorganic ions, and water quality in hydroponic farms in Chollabuk - do. pH range was shown from 6.00 to 7.65 and the average was 6.58. EC range was shown from 0.01 to 0.94 mS/cm and the average was 0.28 mS/cm. Na concentration ranged from 4.71 to 145.44 ppm and Cl concentration ranged from 0 to 171.80 ppm were shown and their average were 24.24 ppm and 26.33 ppm, respectively. Na and Cl concentrations were higher as 7.7%, compared to those of other farms, which could cause the salt accumulation in the substrate mats. K, Ca and Mg concentrations were ranged from 0.93 to 17.38 ppm, 2.29 to 34.30 ppm and 0.70 to 18.61 ppm, respectively. Average concentrations were 3.06 ppm in K, 13.00 ppm in Ca and 4.91 ppm in Mg. 50, and PO$_4$ concentration ranged from 0.63 to 59.79 ppm and 0 to 4.28 ppm were shown and their average were 18.11 and 0.51 ppm. Cu concentration was ranged from 0 to 0.32 ppm and 0 to 6.22 ppm in Zn concentration. Their average were 0.02 ppm and 0.52ppm, respectively.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.80-88
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• 1995

This survey has been conducted, mostly in inorganic ions, to get some basic data for the culture solution composition, analyzing water quality of some hydroponic farms. pH range was shown from 5.95 to 7.61 and the average of 6.75. Relatively wide range of EC, from 0.07 to 0.97 mS/cm and the average of 0.35 mS/cm were obtained. 19.5 percent of farms investigated showed over 0.5 mS/cm of EC, which means more careful culture solution composition and its management are needed in these farms. Na concentration ranged from 5.0 to 41.4 ppm and Cl concentration ranged from 10 to 99 ppm were shown and their average were 20.38 ppm and 35.16 ppm, respectively. Higher Na concentration compared to standard of 11.5 ppm was shown in 75% of farms and Higher Cl concentration compared to standard of 35.5 ppm was shown in 33.3% of farms. These concentration were considered rather high, which can cause salt accumulation in substrate mats. Ca and Mg concentrations were ranged from 1.60 to 131 ppm and 0.96 to 34.1 ppm, respectively. Average concentrations were 26.11 ppm in Ca and 8.10 ppm in Mg. In case of HCO$_3$, 24 to 295 ppm of concentration range and average of 63.13 ppm were obtained. Fe range was 0.01 to 0.87 ppm and its average was 0.14 ppm. This results showed that Fe elimination was necessary in well water.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.69-77
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• 2006

The experiment was conducted to investigate the nutrition absorption pattern in the growth stage and develope the optimal nutrient solution hydroponically grown the cherry tomato 'Koko' in closed substrate culture system with the nutrient solution of National Horticultural Research Station in Japan into 1/2S, 1S, and 2S. When plant was grown in 1/2S, the growth and yield were high and the pH and EC in the root zone were stable. Suitable composition of nutrient solution for cherry tomato was $NO_3-N\;6.8,\;PO_4-P\;2.7,\;K 3.2,\;Ca\;3.6\;and\;Mg\;1.1\;me\;L^{-1}$ in the early growth stage, $NO_3-N\;7.3,\;PO_4-P\;2.2,\;K\;3.7,\;Ca\;3.6;and\;Mg\;1.1\;me\;L^{-1}$ in the late growth stage by calculating a rate of nutrient and water uptake. To estimate the suitability for the nutrient solution in a development of cherry tomato developed by Wongkwang university in Korea (WU), plant was grown in perlite substrate supplied with different solution and strengths(S) by research station for greenhouse vegetable and floriculture in the Netherlands (Proefstation voor tuinbouw onder glas th Mssldwijk; PTG) of 1/2S, 1S, and 2S, respectively. The growth was good at the PTG and WU 2S in the early stage and the PTG of 1S and WU of 1S and 2S in the late stage. The highest yield of cherry tomato obtained in the WU of 2S. pH and EC in root zone of WU of 2S were stable during the early and late growth stage. Therefore when cherry tomato plant was grown in WU of 2S of EC $1.6{\sim}2.0\;dS\;m^{-1}$in the nutrient solution, not only stable growth and yield but also fertilizer reduction can be obtained than that of PTG.

• Journal of Bio-Environment Control
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• v.11 no.4
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• pp.168-174
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• 2002

A new circulating hydroponic system was invented for oriental melons grown in the greenhouse. For developing nutrient solution management techniques, we examined the changes of nutrient contents of circulating solution in three different types of new hydroponic systems. The yield and fruit quality of oriental melons in Hydroponics were better than those in soil culture. The substrate culture was appropriate fer hydroponics of oriental melons, and NFT was turned to be the opposite due to the physiological disorder during hot seasons. Yamazaki's melon solution with EC 2.0dS.m$^{[-10]}$ was the most appropriate for oriental melons. The new circulating hydroponic system seemed to be appropriate for oriental melons because of the stable EC, pH and the macro- and micro-element contents. NO$_3$-N, Ca and Mg contents in the circulating solution kept a good balance in all types of hydroponics. However, p content, compared to other types, decreased by the degree of 1 me.L$^{[-10]}$ in perlite medium. K content showed irregular status in perlite but showed the stable status in cocopeat. Generally, microelements, except Mo, showed stable absorption in the substrate culture. However, in NET, most of the elements showed irregular absorption except B and Mn. Microelement absorption, especially Cu, Zn and Mo, decreased during hot seasons.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.70-76
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• 2003

Continuous anaerobic hydrogen production with a mixed culture was investigated. With a sucrose concentration of 5g COD/L in the feed, hydrogen production exceeded $0.5mole\;H_2/mole\;hexose$ was found at the early stage, however it did not maintain longer than 9days. It was assumed that the failure was caused by insufficient active hydrogen producing bacteria in the reactor. Therefore, effects of pH control, repeated heat treatment and substrate concentration on sustainable continuous anaerobic hydrogen production was examined to find out operating conditions to sustainable hydrogen production. Decrease of hydrogen production was not overcome by only pH control at 5.3. Repeated heat treatment could recover hydrogen producing activity without any external inoculum supply. However, frequent heat treatment was needed because the treated sludge also showed the tendency in decrease of hydrogen production. With a sucrose concentration of 30g COD/L in the feed, hydrogen production maintained $1.0-1.4mole\;H_2/mole\;hexose$ in continuously stirred tank reactor and $0.2-0.3mole\;H_2/mole\;hexose$ in anaerobic sequencing batch reactor) for 24days. More than 90% of soluble organics in effluent was organic acids, in which n-butyrate was the most one.