• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.443-448
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• 2008

Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline cellulose (Avicel), the reporter protein is guided to the cellulose fibers that are increasingly exposed as the scouring process proceeds. Among the tested reporter proteins, a thermostable ${\beta}$-glycosidase (BglA) from Thermus caldophilus was found to be most appropriate, showing a higher applicability and stability than GFP, DsRed2, or a tetrameric ${\beta}$-glycosidase (GUS) from Escherichia coli, which were precipitated more seriously during the expression and purification steps. When cotton fabrics with different scouring levels were treated with the BglA-CBD and incubated with X-Gal as the chromogenic substrate, an indigo color became visible within 2 h, and the color depth changed according to the conditions and extent of the scouring.

• 한국환경과학회지
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• 제9권4호
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• pp.351-367
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• 2000

Air-lift biofilm reactor should be an admirable process substituting conventional activated sludge process, because of its small area requirement as well as high volumetric loading capacity and stability against loading and chemical shocks. However most of the past research on the performance of ABR was focused on the sewage treatment. This research studied the applicability of ABR to treat high strength wastewater. A bench-scale ABR was operated to treat high strength synthetic wastewater, tannery wastewater and petrochemical wastewater, and its applicability was conclusive In case of synthetic wastewater, ABR showed good performance in which the substarate removal efficiency was higher that 80% even under short HRT(1.4 hr) and high volumetric loading rate(9.3 kgCODcr/$m^3$.day). When ABR was applied to treat tannery wastewater, it was suggested that the maximum volumetric loading rate and F/M ratio should be 7.7kgCODcr/$m^3$.day, 0.76 $day^{-1}$, respectively. And high substrate removal efficiency over than 90 % was observed with 4,000 mgCODcr/L of petrochemical wastewater. Even though effluent concentration was quite high, ABR should be applicable to treat the high strength wastewater, because of its high loading capacity.

• Transactions on Electrical and Electronic Materials
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• 제13권2호
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• pp.73-77
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• 2012

Operation of liquid crystal displays (LCDs) can be improved by monolithic integration of the pixel transistors with light emitting diodes (LEDs) on a single substrate. Conventional LCDs make use of filters to control the backlighting which reduces the overall efficiency. These LCDs also utilize LEDs in series which impose failure and they require high voltage for operation with a power factor correction. The screen of small hand-held devices can operate from moderate brightness. Therefore, III-V nanowires that are grown along with transistors over Silicon substrates can be utilized. Control of nanowire LEDs with nanowire transistors will significantly lower the cost, increase the efficiency, improve the manufacturing yield and simplify the structure of the small displays that are used in portable devices. The steps to grow nanowires on Silicon substrates are described. The vertical n-type and p-type nanowire transistors with surrounding gate structures are characterized. While biased at 0.5 V, nanowire transistors with minimum radius or channel width have an OFF current which is less than 1pA, an ON current more than 1 ${\mu}A$, a total delay less than 10 ps and a transconductance gain of more than 10 ${\mu}A/V$. The low power and fast switching characteristics of the nanowire transistor make them an ideal choice for the realization of future displays of portable devices with long battery lifetime.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 추계학술대회 초록집
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• pp.58.1-58.1
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• 2010

Stainless Steel의 유연한 기판을 사용하여 ZnO:Al/Ag의 후면전극에서 Ag 증착 실험조건 변화를 통해서 light trapping을 개선한 n-i-p 구조의 플렉서블 미세결정질 실리콘 박막 태양전지를 제작하였다. 실험 방법으로 마그네트론 스퍼터를 사용하여 Stainless Steel 기판 위에 ZnO:Al/Ag를 증착하여 후면전극으로 사용되는 back reflector를 제작하였으며 그 위에 미세결정질 실리콘 박막을 증착하였다. Back reflector에서 Ag 박막의 증착 온도가 증가할수록 표면결정 성장으로 roughness가 증가하여 반사도를 증가하였다. 또한, Ag 박막 증착 두께와 압력 변화에 따른 광학적 특성변화를 Atomic Force Microscope(AFM), Scanning Electron Microscopy(SEM),UV-visible-nIR spectrometry로 조사하여 최적의 조건을 찾았으며 개선된 back reflector의 특성이 n-i-p 구조의 플렉서블 미세결정질 실리콘 박막 태양전지에 적용하여 light trapping의 증가가 태양전지에서 광학적인 특성 변화 및 효율 향상에 영향을 주는지 Photo IV와 EQE(External Quantum Efficiency)를 통하여 조사하였다.

• 한국재료학회지
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• 제28권3호
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• pp.159-165
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• 2018

In order to increase the efficiency of the sputtering method widely used in thin film fabrication, a dc sputtering apparatus which supplies both high frequency and magnetic field from the outside was fabricated, and cobalt thin film was fabricated using this apparatus. The apparatus can independently control the applied voltage, the target-substrate distance, and the target current, which are important parameters in the sputtering method, so that a stable glow discharge is obtained even at a low gas pressure of $10^{-3}$ Torr. The fabrication conditions using the sputtering method were mainly performed in $Ar+O_2$ mixed gas containing about 0.6 % oxygen gas under various Ar gas pressures of 1 to 30 mTorr. The microstructure of Co thin films deposited using this apparatus was examined by electron diffraction pattern and X-ray techniques. The magnetic properties were investigated by measuring the magnetization curves. The microstructure and magnetic properties of Co thin films depend on the discharge gas pressure. The thin film fabricated at high gas pressure showed a columnar structure containing a large amount of the third phase in the boundary region and the thin film formed at low gas pressure showed little or no columnar structure. The coercivity in the plane was slightly larger than that in the latter case.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2011년도 제42회 하계학술대회
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• pp.1485-1486
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• 2011

A layer of $TiO_2$ thin film less than ~500nm in thickness, as a blocking layer, was coated by sol-gel method directly onto the anode electrode to be isolated from the electrolyte in dye-sensitized solar cells (DSCs). This is to prevent the electrons from back-transferring from the electrode to the electrolyte (I-/I3-). The effects of heat treatment conditions of the gel and as-coated film on the thickness and consolidation to substrate were studied. The flexible DSCs were fabricated with working electrode of Ti thin foil coated with blocking $TiO_2$ layer, dye-attached mesoporous $TiO_2$ film, gel electrolyte and counter electrode of Pt-deposited indium doped tin oxide/polyethylene naphthalate (ITO/PEN). The photo-current conversion efficiency of the cell was 5.3% ($V_{oc}=0.678V$, $J_{sc}=12.181mA/cm^2$, ff=0.634) under AM1.5, 100 mW/$cm^2$ illumination.

• 전기학회논문지
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• 제59권3호
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• pp.588-593
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• 2010

Sputter deposition on a Pt counter electrode was studied using radio frequency (RF) plasma as the improvement of incident photon to current conversion efficiency (IPCE) for dye-sensitized solar cells (DSCs). Effects of the sputtering thickness and the incident angle on a Pt counter electrode for DSCs were investigated. Experiments to get the optimal sputtering time for the performance of the DSCs were carried out. And it is found that the optimized sputtering time was 120 seconds, in addition, the incident angles of the substrate was adjusted from $0^{\circ}$ to $60^{\circ}$. The maximum efficiency of 5.37% was obtained at the incident angle of $40^{\circ}$ with an active cell area of $1cm^2$.

• 상하수도학회지
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• 제24권6호
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• pp.703-712
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• 2010

This study performed to extract operation factors of major organic wastes, which were food wastes and waste activated sludge generated in industries in order to use them as a substrate for bio-H2 production. According to the results of experimental analysis for hydrogen production capacity by various organic concentrations, the hydrogen production yield was the highest at 80 g/L, and the efficiency was improved by the pretreatment of waste activated sludge (acid treatment, alkali treatment). Hydrogen production efficiency was improved by mixing food wastes and waste activated sludge if waste activated sludge was below than 30%, however, it was decreased when it was more than 50%. The impacts of heavy metals on the hydrogen production shows that the inhibition level depends on the concentration of Cr, Zn, and Cu, Fe was able to enhance the hydrogen production.

• 한국전기전자재료학회논문지
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• 제26권4호
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• pp.298-303
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• 2013

In this study, the W-interconnected dye-sensitized solar cell (DSSC) modules composed of a number of rectangular cells connected in series were investigated, where neighboring cells are processed in reverse. The DSSC modules, a module of dimension about 200 mm ${\times}$ 200 mm, were fabricated with different working electrode width ranging from 5 mm to 21 mm. The short-circuit current of the module increased as the working electrode width increased. Whereas, the decrease in the working electrode width resulted in the increase of the conversion energy efficiency, fill factor, and open-circuit voltage, which is explained by the fact that the possibility that electrons are recombined along their path on the transparent conductive oxide substrate decreases. The module with the conversion energy efficiency of 3.59% was obtained with the working electrode width of 5 mm.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.318-324
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• 1998

A ${\beta}$-galactosidase with high transgalactosylic activity was purified from a Bacillus species, registered as KFCC10855. The enzyme preparation showed a single protein band corresponding to a molecular mass of 150 kDa on SDS-PAGE and gave a single peak with the estimated molecular mass of 250 kDa on Sephacryl S-300 gel filtration, suggesting that the enzyme is a homodimeric protein. The amino acid and sugar analyses revealed that the enzyme is a glycoprotein, containing 19.2 weight percent of sugar moieties, and is much more abundant in hydrophilic amino acid residues than in hydrophobic residues, the mole ratio being about 2:1. The pI and optimum pH were determined to be 5.0 and 6.0, respectively. Having a temperature optimum at $70^{\circ}C$ for the hydrolysis of lactose, the enzyme showed good thermal stability. The activity of the enzyme preparation was markedly increased by the presence of exogenous Mg (II) and was decreased by the addition of EDTA. Among the metal ions examined, the most severely inhibitory effect was seen with Ag (I) and Hg (II). Further, results of protein modification by various chemical reagents implied that 1 cysteine, 1 histidine, and 2 methionine residues occur in certain critical sites of the enzyme, most likely including the active site. Enzyme kinetic parameters, measured for both hydrolysis and transgalactosylation of lactose, indicated that the enzyme has an excellent catalytic efficiency for formation of the transgalactosylic products in reaction mixtures containing high concentrations of the substrate.