• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.157-165
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• 2019

Di- and tri-methylation of lysine 4 on histone H3 (H3K4me2 and H3K4me3, respectively) are epigenetic markers of active genes. Complex associated with Set1 (COMPASS) mediates these H3K4 methylations. The involvement of COMPASS activity in secondary metabolite (SM) biosynthesis was first demonstrated with an Aspergillus nidulans cclA knockout mutant. The cclA knockout induced the transcription of two cryptic SM biosynthetic gene clusters, leading to the production of the cognate SM. Monascus spp. are filamentous fungi that have been used for food fermentation in eastern Asia, and the pigment Monascus azaphione (MAz) is their main SM. Monascus highly produces MAz, implying that the cognate biosynthetic genes are highly active in transcription. In the present study, we examined how COMPASS activity modulates MAz biosynthesis by inactivating Monascus purpureus cclA (Mp-cclA) and swd1 (Mp-swd1). For both ${\Delta}Mp-cclA$ and ${\Delta}Mp-swd1$, a reduction in MAz production, accompanied by an abated cell growth, was observed. Suppression of MAz production was more effective in an agar culture than in the submerged liquid culture. The fidelity of the ${\Delta}Mp-swd1$ phenotypes was verified by restoring the WT-like phenotypes in a reversion recombinant mutant, namely, trpCp: Mp-swd1, that was generated from the ${\Delta}Mp-swd1$ mutant. Real-time quantitative Polymerase chain reaction analysis indicated that the transcription of MAz biosynthetic genes was repressed in the ${\Delta}Mp-swd1$ mutant. This study demonstrated that MAz biosynthesis is under the control of COMPASS activity and that the extent of this regulation is dependent on growth conditions.

• Journal of Ocean Engineering and Technology
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• v.36 no.5
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• pp.313-325
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• 2022

Recently around the world, coastal erosion is paying attention as a social issue. Various constructions using low-crested and submerged structures are being performed to deal with the problems. In addition, a prediction study was researched using machine learning techniques to determine the wave attenuation characteristics of low crested structure to develop prediction matrix for wave attenuation coefficient prediction matrix consisting of weights and biases for ease access of engineers. In this study, a deep neural network model was constructed to predict the wave height transmission rate of low crested structures using Tensor flow, an open source platform. The neural network model shows a reliable prediction performance and is expected to be applied to a wide range of practical application in the field of coastal engineering. As a result of predicting the wave height transmission coefficient of the low crested structure depends on various input variable combinations, the combination of 5 condition showed relatively high accuracy with a small number of input variables defined as 0.961. In terms of the time cost of the model, it is considered that the method using the combination 5 conditions can be a good alternative. As a result of predicting the wave transmission rate of the trained deep neural network model, MSE was 1.3×10^-3, I was 0.995, SI was 0.078, and I was 0.979, which have very good prediction accuracy. It is judged that the proposed model can be used as a design tool by engineers and scientists to predict the wave transmission coefficient behind the low crested structure.

• Membrane Journal
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• v.32 no.6
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• pp.465-474
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• 2022

The suction pressure was measured with respect to operational time by the backwashable flat sheet membrane module in membrane bioreactor (MBR). The membrane module having the nominal pore size of 0.2 ㎛ and the effective membrane area of 128cm² was submerged in MLSS 8,000 mg/L active sludge aqueous solution. The suction pressure was observed with respect to permeation flux and the quorum quenching (QQ) treatment. The effects of FR and SFCO operation methods were compared and analyzed in the experimental groups: vacant bead (VB), BH4 and DKY-1 beads. The suction pressure reduction was the most effective for the permeation flux 40 L/m² ⋅h with the injection of DKY-1 QQ beads. Also, the suction pressure reduction by the backwashing method was more than twice for using DKY-1 QQ beads.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.49 no.1
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• pp.43-48
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• 2023

The biocompatibility and durability of implant fixtures are major concerns for dentists and patients. Mechanical complications of the implant include abutment screw loosening, screw fracture, loss of implant prostheses, and implant fracture. This case report aims to describe management of a case of fixture damage that occurred after screw fracture in a tissue level, internal connection implant and microscopic evaluation of the fractured fixture. A trephine bur was used to remove the fixture, and the socket was grafted using allogeneic bone material. The failed implant was examined by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), which revealed a fractured fixture with both normal and irregular bone patterns. The SEM and EDS results give an enlightenment of the failed fixture surface micromorphology with microfracture and contaminated chemical compositions. Noticeably, the significantly high level of gold (Au) on the implant surface and the trace amounts of Au and titanium (Ti) in the bone tissue were recorded, which might have resulted from instability and micro-movement of the implant-abutment connection over an extended period of time. Further study with larger number of patient and different types of implants is needed for further conclusion.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1238-1249
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• 2023

In this study, we sought to investigate the production and optimization of biosurfactants by soil fungi isolated from petroleum oil-contaminated soil in Saudi Arabia. Forty-four fungal isolates were isolated from ten petroleum oil-contaminated soil samples. All isolates were identified using the internal transcribed spacer (ITS) region, and biosurfactant screening showed that thirty-nine of the isolates were positive. Aspergillus niger SA1 was the highest biosurfactant producer, demonstrating surface tension, drop collapsing, oil displacement, and an emulsification index (E₂₄) of 35.8 mN/m, 0.55 cm, 6.7 cm, and 70%, respectively. This isolate was therefore selected for biosurfactant optimization using the Fit Group model. The biosurfactant yield was increased 1.22 times higher than in the nonoptimized medium (8.02 g/l) under conditions of pH 6, temperature 35℃, waste frying oil (5.5 g), agitation rate of 200 rpm, and an incubation period of 7 days. Model significance and fitness analysis had an RMSE score of 0.852 and a p-value of 0.0016. The biosurfactant activities were surface tension (35.8 mN/m), drop collapsing (0.7 cm), oil displacement (4.5 cm), and E₂₄ (65.0%). The time course of biosurfactant production was a growth-associated phase. The main outputs of the mathematical model for biomass yield were Yx/s (1.18), and µ_max (0.0306) for biosurfactant yield was Y_p/s (1.87) and Y_p/x (2.51); for waste frying oil consumption the So was 55 g/l, and Ke was 2.56. To verify the model's accuracy, percentage errors between biomass and biosurfactant yields were determined by experimental work and calculated using model equations. The average error of biomass yield was 2.68%, and the average error percentage of biosurfactant yield was 3.39%.

• The Journal of the Acoustical Society of Korea
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• v.29 no.7
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• pp.448-457
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• 2010

Vibrations and wave propagations in waveguide structures can be analysed efficiently by using waveguide finite element (WFE) method. The WFE method only models the 2-dimensional cross-section of the waveguide with finite elements so that the size of the model and computing time are much less than those of the 3-dimensional FE models. For cylindrical shells or pipes which have simple cross-sections, the external coupling with fluids can be treated theoretically. For waveguides of complex cross-sectional geometries, however, numerical methods are required to deal with external fluids. In this numerical approach, the external fluid is modelled by the boundary elements (BEs) and connected to WFEs. In order to validate this WFE/BE method, a pipe submerged in water is considered in this study. The dispersion diagrams and point mobilities of the pipe simulated are compared to those that theoretically obtained. Also the acoustic powers radiated from the pipe are predicted and compared in both cases of air and water as an external medium.

• Journal of the Society of Naval Architects of Korea
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• v.32 no.1
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• pp.118-131
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• 1995

The shock fracture analysis for the structures of navy vessels subject to underwater explosions or of high speed vessels frequently subject to impact loads has been carried out in two steps such as the global or macro analysis and the fine or micro analysis. In the macro analysis, Doubly Asymptotic Approximation(DAA) has been applied. The three main failure modes of structure members subject to strong shock loading are late time fracture mode such as plastic large deformation mainly due to dynamic plastic buckling, and the early time fracture mode such as tensile tearing failure or transverse shear failure. In this paper, the tensile tearing failure mode is numerically analyzed for the micro analysis by calculating the dynamic stress intensity factor $K_I(t)$, which shows the relation between stress wave and crack propagation on the longitudinal stiffener of the model. Especially, in calculating this factor, the numerical caustic method developed from shadow optical method of caustic well known as experimental method is used. The fully submerged vessel is adopted for the macro analysis at first, of which the longitudinal stiffener, subject to early shock pressure time history calculated in macro analysis, is adopted for the micro analysis.

• Journal of Periodontal and Implant Science
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• v.47 no.5
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• pp.312-327
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• 2017

Purpose: This study assessed marginal bone remodeling and soft tissue esthetics after the loading of single bone-level implants in the anterior maxilla. Methods: An open, single-arm observational clinical trial with 3 years of follow-up was performed, including 22 implants. The patients presented with a single tooth gap in the anterior maxilla (tooth positions 14-24), with natural or restored adjacent teeth. An implant was placed at least 8 weeks post-extraction and healed submerged for 6 weeks. After the second-stage operation, a fixed provisional prosthesis was provided. The final restoration was placed 6 months after the provisional restoration. The time of the provisional crown connection was considered to be the baseline in this study. Esthetic parameters and the marginal bone level were assessed at 6, 12, 24, and 36 months. Results: All implants were well integrated in the bone. A statistically significant increase was found in the mean implant stability quotient between the time of the provisional prosthesis and the time of the final prosthesis. Most implants (95.5%) revealed marginal bone resorption (<0.5 mm), and just 1 implant (4.5%) showed a change of 2.12 mm from baseline to 36 months (mean $0.07{\pm}0.48mm$), while the crestal bone level decreased significantly, from $2.34{\pm}0.93mm$ at baseline to $1.70{\pm}1.10mm$ at 36 months. The facial gingival margin and papilla were stable and the esthetic scores indicated high patient and dentist satisfaction. Conclusions: Platform-switching bone-level implants placed in maxillary single-tooth gaps resulted in successful osseointegration with minimal marginal bone resorption. The peri-implant soft tissue was also esthetically satisfying and stable.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.29 no.1
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• pp.263-271
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• 2005

The most important point when we engage on waterway dredging work is supplying safe navigational passage to the vessels underway by narrowing dredge work area and removing submerged dangers. In order to meet this end it is neccessary to use auxiliary equipment for shifting actively and mooring and adopt automation of dredging work by integrating information on real time position, dredging depth, and work information. The danger with a spud control system in this study, by the way, is able to employed on continuous dredging work with the narrowest working area allowing wide and safe passages to vessels underway, by moving the dredger to the working zone with the spud controlled automatically. Furthermore, it has been improved definitely compared with the existing dredging proccess management system such that it shows the track of spud and working depth on the electronic navigation chart of window, together with the final outcome of dredging work. The test dredging work at the entrance of Busan North Port for system evaluation showed that actual working time available was twice of the one by the existing anchor system, and that it reduced 38% of time for preparation work and one man power.

• Journal of Navigation and Port Research
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• v.29 no.5 s.101
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• pp.395-402
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• 2005

The most important point when we engage on waterway dredging work is supplying safe navigational passage to the vessels underway by narrowing dredge work area and removing submerged dangers. In order to meet this end it is necessary to use auxiliary equipment for shifting actively and mooring and adopt automation of dredging work by integrating information on real time position, dredging depth, and work information. The dredger with a spud control system in this study, by the way, is able to employed on continuous dredging work with the narrowest working area allowing wide and safe passages to vessels underway, by moving the dredger to the working zone with the spud controlled automatically. Furthermore, it has been improved definitely compared with the existing dredging process management system such that it shows the track of spud and working depth on the electronic navigation chart of window, together with the final outcome of dredging work. The test dredging work at the entrance of Busan North Port for system evaluation showed that actual working time available was twice of the one by the existing anchor system, and that it reduced $38\%$ of time for preparation work and one man power.