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Distribution and Ecology of Marsh Clam in Gyeongsangbuk-do II. Reproductive Cycle and Larval Development of the Corbicula japonica (경상북도 재첩자원 분포 및 생태 조사 II. 일본재첩 Corbicula japonica의 생식주기 및 유생발생)

  • 변경숙;정의영
    • The Korean Journal of Malacology
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    • v.17 no.1
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    • pp.45-55
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    • 2001
  • Gametogenes, reproductive cycle, first sexual maturity(biological minimum size), sex ratio and larval development of the marsh clam Corbicula japonica were investigated monthly by histological observations. Samples were collected in brackish water of Gokgang stream, Kyungsangbuk-Do, Korea, from August 1997 to July 1998. Sexuality of Corbicula japonica is dioecious and the species are an oviparous clam. The gonads are irregularly arranged from the sub-region of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of ovarian sac which are branched arborescent. Oogonia actively proliferate along the germinal epithelium of ovarian sac, in which young oocytes are growing. The testis is composed of a number of testicular tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between developing oocytes and spermatocytes in the early developmental stages. With the further development of the ovary and testis these tissue and cells gradually disappear. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells are considered to be related to the growing of the oocytes and spermatocytes. The spawning period is from July to September, and the main spawning occur between July and August when seawater temperatures reach above 22$^{\circ}C$. The reproductive cycle of this species can be divided into five successive stages; early active (February to April), late active (May to July), ripe (June to September), partially spawned (July to September), degenerative (September to October) and resting stage (October to February). Percentages of first sexual maturity of female and male clams ranging in length from 10 mm to 12 mm are over 50% and 100% for clams over 16.0 mm in shell length. Fertilized eggs or Corbicula japonica were 80-90 ${\mu}{\textrm}{m}$ in diameter. In the early embryonic development of C. japonica, the appearance of polar body, trochophore and D-shaped veliger were observed around 40 min., 27 hours and 4 days after spawning, respectively, at a water temperature of 26.5-28.$0^{\circ}C$. The size of larvae of early umbo stage was about 185-210 ${\mu}{\textrm}{m}$ in shell length, 160-180 ${\mu}{\textrm}{m}$ in shell height around 7 days after fertilization. The correlation of relative growth between the culture day (D) and shell length (SL) was expressed by the following simple formula from D-shaped veliger to metamorphosing stage; SL = 13.300D + 209.36($r^2$= 0.9078).

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Molecular Epidemiology and Antimicrobial Resistance of Methicillin-resistant Staphylococcus aureus Isolated from Nasal Swab at Intensive Care Unit (중환자실 입원 환자의 비강 도말에서 메티실린 내성 황색포도알균의 분자역학, 항생제 내성 연구)

  • Kwak, Om Sub;Kwon, Mee Hye;Jeong, Ji Hyun;Kang, Mi-il;Cheun, Ji Young;Lee, Go Eun;Kim, Young Keun;Choi, Eu Gene;Na, Moon Jun;Kwon, Hee Uk;Son, Ji Woong
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.2
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    • pp.91-98
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    • 2008
  • Background: Methicillin-resistant Staphylococcus aureus (MRSA) is the most common organism associated with nosocomial infections. MRSA infections are becoming increasing important because they have emerged no only as healthcare-associated (HA) infections but also as community-associated (CA) ones. This study examined the moleculo-epidemiology of MRSA, which was isolated from nasal swabs in the intensive care unit (ICU) at Konyang University Hospital. MRSA are classified into HA-MRSA and CA-MRSA. Methods: From June to September 2006, 353 patients who were admitted to the ICU in Konyang University Hospital were enrolled in this study. Single nasal swabs were obtained for culture in the ICU on the 1st day. Pulsed-field gel electrophoresis and the antimicrobial resistant patterns were analyzed between HA- and CA-MRSA. An antimicrobial sensitivity test was also performed. Results: Forty two strains of MRSA were isolated from 353 patients (11.9%). Among the 42 isolates, HA-MRSA and CA-MRSA were found in 33 (78.6%), and 9 (21.4%), respectively. Eleven different PFGE types (type A to K) were identified. Types A (n=9) and B (n=7) were the most common for HA-MRSA, and types A (n=2) and B (n=2) were identified in CA-MRSA. The proportion of types A and B in CA-MRSA (44.4%) was similar to that in HA-MRSA (48.5%). The rates of resistance rates to erythromycin and ciprofloxacin were higher in HA-MRSA than in CA-MRSA. Conclusion: The rate of isolation of MRSA in an ICU setting was 11.9%. HA-MRSA was isolated more frequently than CA-MRSA. The rate of resistance of HA-MRSA to erythromycin and ciprofloxacin was higher than that of CA-MRSA. Despite the small number of subjects, the main isolates (type A and B) of CA-MRSA were similar to those of HA-MRSA.

A Study on Students' Recognitions of the Manners of Offering Congratulation or Condolence in Social Life and their Requirements for Education on Such Manners - Based on Four-Year Universities in Seoul - (사회생활의 경조사예절에 대한 대학생의 인식 및 교육 요구연구 - 서울시내 4년제 대학을 중심으로 -)

  • Kim, Eun-Kyum;Choi, Bae-Young
    • Journal of Family Resource Management and Policy Review
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    • v.12 no.2
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    • pp.153-171
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    • 2008
  • This study examined university students' view on the importance of attending rituals of congratulation or condolence in social life, and the extent to which they recognized the manners of offering congratulation or condolence. It also looked into the plans and content of education on such manners required by the students. The study aimed at seeking a direction for education on the manners of offering congratulation or condolence in order to help the students acquire an appropriate conception of offering congratulation or condolence and contribute to firmly establishing a sound culture of conveying congratulation or condolence. The following results were obtained in the study. 1. One a five-point scale, university students' recognitions of the importance of attending rituals of congratulation or condolence were revealed as follows: attending condolence rituals(4.55) > attending wedding ceremonies(4.30) > visiting sick acquaintances(4.03) > attending birthday parties (3.50) > attending openings(3.47) > attending promotion or retirement parties(3.42). 2. One a five-point scale, university students' recognitions of the manners of offering congratulation or condolence in social life were as follows: the manners of attending condolence rituals(4.20) > the manners of attending wedding ceremonies(4.06) > the manners of attending promotion or retirement parties(3.9) > the manners of attending openings(3.80) > the manners of attending birthday parties(3.69) > the manners of visiting sick acquaintances(3.44). 3. One a five-point scale, university students' requirements for the content of education on the manners of conveying congratulation and condolence were as follows: education on the manners of attending condolence rituals(4.08) > education on the manners of visiting sick acquaintances(3.35) > education on the manners of attending wedding ceremonies(3.27) > education on the manners of attending promotion and retirement parties(2.96) > education on the manners of attending birthday parties(2.91) > education on the manners of attending openings(2.91). 4. One a five-point scale, university students' requirements for the plan for education on the manners of conveying congratulation and condolence were as follows: 'The education content should be applicable in real life'(4.42) > 'The education content should be easily understandable' (4.23) > 'Information or materials related to the education content should be easily accessible'(4.01) > 'The theory and actual practice of education should be taught in parallel' (3.96) > 'Education related materials should be utilized'(3.92) > 'Students' demands should be reflected when structuring the content of education'(3.91). 5. The study examined the relationship among university students' recognitions of the importance of attending rituals of congratulation or condolence, their recognitions of the manners of offering congratulation or condolence, and their demands regarding such manners. According to the results, a static relationship was found in all sub-areas, indicating students show higher recognition of practicing manners of conveying congratulation or condolence as they have higher recognition of attending rituals of congratulation or condolence in social life. In addition, higher recognition of practicing manners of conveying congratulation or condolence made them demand more for the necessity of plan and content of education on the manners of offering congratulation or condolence in university.

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Roles of Perceived Use Control consisting of Perceived Ease of Use and Perceived Controllability in IT acceptance (정보기술 수용에서 사용용이성과 통제가능성을 하위 차원으로 하는 지각된 사용통제의 역할)

  • Lee, Woong-Kyu
    • Asia pacific journal of information systems
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    • v.18 no.2
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    • pp.1-14
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    • 2008
  • According to technology acceptance model(TAN) which is one of the most important research models for explaining IT users' behavior, on intention of using IT is determined by usefulness and ease of use of it. However, TAM wouldn't explain the performance of using IT while it has been considered as a very good model for prediction of the intention. Many people would not be confirmed in the performance of using IT until they can control it at their will, although they think it useful and easy to use. In other words, in addition to usefulness and ease of use as in TAM, controllability is also should be a factor to determine acceptance of IT. Especially, there is a very close relationship between controllability and ease of use, both of which explain the other sides of control over the performance of using IT, so called perceived behavioral control(PBC) in social psychology. The objective of this study is to identify the relationship between ease of use and controllability, and analyse the effects of both two beliefs over performance and intention in using IT. For this purpose, we review the issues related with PBC in information systems studies as well as social psychology, Based on a review of PBC, we suggest a research model which includes the relationship between control and performance in using IT, and prove its validity empirically. Since it was introduced as qa variable for explaining volitional control for actions in theory of planned behavior(TPB), there have been confusion about concept of PBC in spite of its important role in predicting so many kinds of actions. Some studies define PBC as self-efficacy that means actor's perception of difficulty or ease of actions, while others as controllability. However, this confusion dose not imply conceptual contradiction but a double-faced feature of PBC since the performance of actions is related with both self-efficacy and controllability. In other words, these two concepts are discriminated and correlated with each other. Therefore, PBC should be considered as a composite concept consisting of self-efficacy and controllability, Use of IT has been also one of important areas for predictions by PBC. Most of them have been studied by analysis of comparison in prediction power between TAM and TPB or modification of TAM by inclusion of PBC as another belief as like usefulness and ease of use. Interestingly, unlike the other applications in social psychology, it is hard to find such confusion in the concept of PBC in the studies for use of IT. In most of studies, controllability is adapted as PBC since the concept of self-efficacy is included in ease of use explicitly. Based on these discussions, we can suggest perceived use control(PUC) which is defined as perception of control over the performance of using IT and composed of controllability and ease of use as sub-concepts. We suggest a research model explaining acceptance of IT which includes the relationships of PUC with attitude and performance of using IT. For empirical test of our research model, two user groups are selected for surveying questionnaires. In the first group, there are freshmen who take a basic course for Microsoft Excel, and the second group consists of senior students who take a course for analysis of management information by Excel. Most of measurements are adapted ones that have been validated in the other studies, while performance is real score of mid-term in each class. In result, four hypotheses related with PUC are supported statistically with very low significance level. Main contribution of this study is suggestion of PUC through theoretical review of PBC. Specifically, a hierarchical model of PUC are derived from very rigorous studies in the relationship between self-efficacy and controllability with a view of PBC in social psychology. The relationship between PUC and performance is another main contribution.

Assessment of Patency of Coronary Artery Bypass Grafts Using Segmented K-space Breath-hold Cine Cardiovascular Magnetic Resonance Imaging: A Clinical Feasibility Study (호흡멈춤상태에서 K-space분할 CINE 자기공명 영상기법을 이용한 관상동맥우회로의 혈류개방성의 검사)

  • Oh-Choon Kwon;Sub Lee;Jong-Ki Kim
    • Investigative Magnetic Resonance Imaging
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    • v.7 no.1
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    • pp.22-30
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    • 2003
  • Purpose : The efficacy of magnetic resonance imaging for evaluating coronary artery disease has been reported. In this study, we evaluated the usefulness of breath-hold segmented K-space cine MR imaging for evaluating the patency of coronary artery bypass grafts (CABG). Materials and Method s : Thirty eight patients with a total of 92 CABGs (36 internal thoracic arteries and 56 saphenous vein grafts) were evaluated using segmented K-space cardiac-gated fast gradient echo sequence (2D-FASTCARD) MR imaging. MR magnitude images were evaluated from the hard copies by two independent observers. A graft was defined as patent if it was seen as a bright small round area on at least two consecutive images throughout the cardiac cycle at a position consistent with the expected location for that graft. Results : MR images were obtained successfully for 23 patients (61%). The sagittal planes were most helpful in visualizing the cross-section of sapheneous vein bypass graft to left circumflex artery branch, whereas the transverse planes were used for identification of internal mammary artery grafts to left anterior descending coronary artery or its branch and identification of saphenous vein grafts to right coronary artery. Forty five grafts were visible using this MR technique, while the grafts were not visible on seven saphenous vein grafts and two internal mammary artery grafts. In two patients showing symptoms of myocardial ischemia, one or two bypass grafts were not visible. Imaging, perpendicular plane to a CABG was important to visualize the flow inside the CABG with maximum sensitivity. Conclusion : Evaluation of patency of the bypass graft was clinically feasible by 2D-FASTCARD MR imaging, whereas any invisible bypass grafts should be further studied by contrast-enhanced MR angiography or by conventional angiography for confirmation of abnormalities.

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Dynamics of Phytoplankton and Zooplankton of a Shallow Eutrophic Lake (lake llgam) (수심이 얕은 부영양 인공호(일감호)의 동 ${\cdot}$ 식물플랑크톤 동태학)

  • Kim, Ho-Sub;Park, Je-Chul;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.36 no.3 s.104
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    • pp.286-294
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    • 2003
  • This study was attempted to understand seasonal dynamics of phyto- and zooplankton communities in shallow, eutrophic Lake llgam and to compare them with the PEG (Plankton Ecology Group) model. Seasonal succession pattern of phytoplankton community was similar to PEG model as Chlorophyceae and Baciliphyceae increase during spring and autumn fellowed by increase of Cyanophyceae. However, based on the cell density and biomass, a dominant phytoplankton community differed with PEG model: Cyanophyceae had been a dominant community throughout a year, except for ice-cover period during which Chlorophyceae was a dominant group. In spring, when ice melted and dissolved nutrients in water column increased, the increase of Chlorophyceae occurred: when nutrients (DIN and DIP) rapidly decreased, Cyanophyceae increase occurred. Microcystis, Oscillatoria, Lyngbya, Merismopedia were maior dominant species of Cyanophyceae and their cell density and/or biomass was the highest in October 2000 (12.9${\pm}$5.8${\times}10^5$ cells/ml, 3.5${\pm}$0.9${\times}10^3{\mu}gC/l$). Cyanophyceae biomass showed positive relationship with chlorophyll a ($r^2$ = 0.71,P< 0.001) and TP concentration ($r^2$ = 0.62, P< 0.001). Small-sized rotifers such as Keratella cochlearis, increased between March and May when Chlorophyceae increased. Both high standing crop of copepods and cladocerans, such as Diaphanosoma brachyrum and Bosmina longirostris occurred between June and September accompanied with the increase of Dinophyceae and Bacillariophyceae. There was no evidence that clear-water phase was caused by zooplankton grazing. The diversity and evenness index of phyto- and/or zooplankton increased with chlorophyll a concentration. These results suggest zooplankton grazing and limiting nutrient deficiency could lead to change of phytoplankton biomass, but not the phytoplankton community in Lake llgam.

Effects of Mulberry-Leaf Powder Tofu Consumption on Serum Lipid Profiles, Ca, Ca/P Ratio and Pb Status in Middle-Aged Women (뽕잎분말 첨가두부 섭취가 비만 중년여성의 혈청 지질, 칼슘, 칼슘/인 비율 및 납 수준변화에 미친 영향)

  • Kim, A.J.;Kim, M.H.;Chung, Kun-Sub
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.432-437
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    • 2006
  • This study was carried out to investigate the effects of mulberry-leaf powder Tofu (MPT) on serum lipids profiles, Ca levels, Ca/P ratio and Pb levels in 30 middle aged obese women living in the Choongnam area. 100 g/day MPT was consumed for 4 weeks. The nutrient contents per 100 g MPT were 86.71 kcal (energy), 8.98 g protein, 0.53 mg fiber, 211.33 mg Ca and 1.59 g fat. Anthropometric measurements, 24 h recall dietary intakes, serum levels of protein, albumin, glucose, Ca and Pb, lipid profiles (cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol), and the Ca/P ratio were analyzed before and after consumption of MPT. After consumption of MPT, there were no significant differences in anthropometric measurements, the levels of serum protein, albumin, glucose, total cholesterol, HDL-cholesterol, lipase activity, HTR (HDL-cholesterol/total cholesterol), CRF (cardiac risk factor), Ca, Ca/P ratio and Pb. There were decreases in the levels of serum triglyceride, LDL-cholesterol, AI (atherogenic index) and LHR (LDL-cholesterol/HDL-cholesterol). Significantly increased dietary intakes of plant protein, total Ca, and plant Ca were observed.

THE RELIABILITY AND VALIDITY OF KOREAN CONNERS PARENT AND TEACHER RATING SCALE (한국어판 Conners 부모 및 교사용 평가 척도의 신뢰도와 타당도에 대한 예비적 연구)

  • Park, Eun-Hee;So, Yu-Kyoung;Choi, Nak-Kyung;Kim, Se-Joo;Noh, Joo-Sun;Ko, Yun-Joo;Kim, Young-Shin
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.14 no.2
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    • pp.183-196
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    • 2003
  • Objectives:The Purpose of this study is to investigate the reliability and validity of Korean Parent and Teacher Conners' Rating Scale. Methods:Randomly selected 1st to 6th graders of the two elementary schools(N=1044) in Anyang City participated in the study. Children diagnosed with ADHD(N=23) at the child and adolescent clinic at a university affiliated hospital were included in the study for the analysis of clinical validity of the scales. Parent and teacher completed Korean Conners' Rating Scale and Korean-ADHD Rating Scale(KARS). In addition, parents completed Korean-Children Behavior CheckList(K-CBCL). Descriptive statistics, t-test, and analysis of variance were performed. Results:Scores of Korean Conners' Parents Rating Scale were significantly correlated with those of Korean Conners Teacher Rating Scale. High internal consistency reliability were demonstrated in both parent and teacher rating scales. There were significant correlations among sub-scales of Conners' Rating Scales, K-CBCL and K-ARS. Factor analyses revealed that the K-CTRS had three-factor structure (Inattention-Passivity, Hyperactivity, Conduct Problem) and the K-CPRS had five-factor structure(Impulsive-Hyperactive, Conduct Problem I, Anxiety, Psychosomatic, Conduct Problem II). Conners' Rating Scales effectively distinguish children with ADHD from children without ADHD. Conclusion:Korean Parent and Teacher Conners' Rating Scales are valid and reliable instruments that are useful for screening and identifying childhood problem behaviors. Future studies are required with a larger number of sample sizes including adolescents from various geographic regions.

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Induction of Apoptosis by Ethanol Extract of Lythrum anceps (Koehne) Makino in Human Leukemia U937 Cells (인체백혈병 U937 세포에서 부처꽃 에탄올추출물에 의한 apoptosis 유도)

  • Jeong, Jin-Woo;Kim, Chul Hwan;Lee, Young-Kyung;Hwang, Yong;Lee, Ki Won;Choi, Kyung-Min;Kim, Jung Il
    • Korean Journal of Plant Resources
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    • v.33 no.4
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    • pp.279-286
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    • 2020
  • Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer effect are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins, depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and -9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent.

A marine deep-towed DC resistivity survey in a methane hydrate area, Japan Sea (동해의 메탄 하이드레이트 매장 지역에서의 해양 심부 견인 전기비저항 탐사)

  • Goto, Tada-Nori;Kasaya, Takafumi;Machiyama, Hideaki;Takagi, Ryo;Matsumoto, Ryo;Okuda, Yoshihisa;Satoh, Mikio;Watanabe, Toshiki;Seama, Nobukazu;Mikada, Hitoshi;Sanada, Yoshinori;Kinoshita, Masataka
    • Geophysics and Geophysical Exploration
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    • v.11 no.1
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    • pp.52-59
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    • 2008
  • We have developed a new deep-towed marine DC resistivity survey system. It was designed to detect the top boundary of the methane hydrate zone, which is not imaged well by seismic reflection surveys. Our system, with a transmitter and a 160-m-long tail with eight source electrodes and a receiver dipole, is towed from a research vessel near the seafloor. Numerical calculations show that our marine DC resistivity survey system can effectively image the top surface of the methane hydrate layer. A survey was carried out off Joetsu, in the Japan Sea, where outcrops of methane hydrate are observed. We successfully obtained DC resistivity data along a profile ${\sim}3.5\;km$ long, and detected relatively high apparent resistivity values. Particularly in areas with methane hydrate exposure, anomalously high apparent resistivity was observed, and we interpret these high apparent resistivities to be due to the methane hydrate zone below the seafloor. Marine DC resistivity surveys will be a new tool to image sub-seafloor structures within methane hydrate zones.