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Cloning and Characterization of a 5-Enolpyruvyl Shikimate 3-Phosphate Synthase (EPSPS) Gene from Korean Lawn Grass (Zoysia japonica) (들잔디 5-Enolpyruvyl Shikimate 3-Phosphate Synthase(EPSPS) 유전자 클로닝 및 특성)

  • Lee, Hye-Jung;Lee, Geung-Joo;Kim, Dong-Sub;Kim, Jin-Beak;Ku, Ja-Hyeong;Kang, Si-Yong
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.648-655
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    • 2010
  • This study is the first comprehensive report on the molecular cloning, structural characterization, sequence comparison between wild and mutant types, copy number in the genome, expression features and activities of a gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Korean lawn grass ($Zoysia$ $japonica$). The full length cDNA of the EPSPS from Korean lawn grass ($zj$EPSPS) obtained from a 3' and 5' RACE method was 1540 bp, containing a 1176 bp ORF, a 144 bp leader sequence (5' UTR) and a 220 bp 3' UTR, which was eventually decoded 391 amino acid residues with a molecular mass of 41.74 kDa. The Southern blot detection of the $zj$EPSPS showed that the gene exists as a single copy in the Korean lawn grass genome. Sequence comparison of the $zj$EPSPS gene demonstrated that the glyphosate-tolerant mutant (GT) having a Pro-53 to Ser substitution in the gene seems to have a preferred binding activity of the enzyme to phosphoenol pyruvate(PEP) over glyphosate, which allows the continuous synthesis of aromatic amino acids in the shikimate pathway. From the Northern blotting analysis, the $zj$EPSPS was found to be highly expressed, with continuous increase until 36 hours after 0.5% glyphosate treatment in both wild and mutant samples, but 1.5-fold higher EPSP synthase activity was observed in the tolerant mutant when exposed to the glyphosate treatment. The molecular information of the $zj$EPSPS gene obtained from this study needs to be further dissected to be more effectively applied to the development of gene-specific DNA markers and zoysiagrass cultivars; nevertheless, the glyphosate-tolerant mutant having the featured $zj$EPSPS gene can be provided to turfgrass managers for weed problems with timely adoptable management options.

Evaluation of the Shear Strength and Stiffness of Frozen Soil with a Low Water Content (함수비가 낮은 동결토의 전단강도 및 강성 평가)

  • Kim, Sang Yeob;Lee, Jong-Sub;Kim, Young Seok;Byun, Yong-Hoon
    • The Journal of Engineering Geology
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    • v.25 no.1
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    • pp.93-102
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    • 2015
  • The characteristics of frozen soils are one of most important factors for foundation design in cold region. The objective of this study is to evaluate the shear strength and stiffness of frozen soils according to the confining conditions during the freezing and shearing phase. A direct shear box is constructed for the frozen specimens and bender elements are mounted on the wall of the shear box to measure shear wave velocities. Specimens are prepared by mixing sand and silt with a silt fraction of 30% in weight and the degree of saturation of 10%, giving a relative density of 60% for all tests. The temperature of the specimens in the freezer is allowed to fall below -5℃, and then direct shear tests are performed. A series of vertical stresses are applied during the freezing and shearing phase. Shear stress, vertical displacement, and shear wave along the horizontal displacement are measured. Experimental results show that in all the tests, shear strength increases with increasing vertical stress applied during the freezing and shearing phases. The magnitude of the increase in shear strength with increasing vertical stress during shearing under fixed vertical stress in the frozen state is smaller than the magnitude of the increase in vertical stress during freezing and shearing. In addition, the change in shear wave velocities varies with the position of the bender elements. In the case of shear waves passing through the shear plane, the shear wave velocities decrease with increasing horizontal displacement. This study provides an evaluation of the properties of shear strength and stiffness of frozen soils under varied confining condition.

Development and Application of the Slope Management Program in Urban Area (대도시 사면관리프로그램 개발 및 적용)

  • Kim, Kyeong-Su;Chae, Byung-Gon;Cho, Yong-Chan;Lee, Choon-Oh;Song, Young-Suk
    • The Journal of Engineering Geology
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    • v.17 no.1 s.50
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    • pp.15-25
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    • 2007
  • In general, the life and asset casualties that occur due to landslide or slope failure in urban areas are larger than that in rural areas. In order to reduce the casualties, a slope management program is necessary to categorize slopes based on properties and to manage them systematically. The slope management system is the establishment of the data base for the geological and geotechnical factor according to slope stability, and the utilization of the data base to manage slopes. The suitable system must develop to slopes in urban area through the survey, analysis and evaluation process. Based on the above necessity, the slope management program which is applicable to slope management in an urban area has been developed at Hwangryung Mt. in Busan as a target area. The developed slope management program has various functions such as slope ID number of each slope or sub-region of a mountain, making a slope data sheet, analysis and grouping of slope stability, and establishment of a data base. The slope management program is constructed by use of GIS, and the survey, test and analysis data according to all slopes can be input and edited into the program. The program can also be utilized practically by end users due to the convenient input, edition printing, management and operation of slope data. Therefore, the slope management system has been established on the application of the developed program in Busan which is located in slope area. As the system is widely applied to other cities, the slope in urban area can be managed systematically and the slope hazards can be minimized.

Leaching and mobility prediction of butachlor, ethoprophos, iprobenfos, isoprothiolane and procymidone in soils (Butachlor, ethoprophos, iprobenfos, isoprothiolane 및 procymidone의 토양 중 용탈과 이동성 예측)

  • Kim, Chan-Sub;Park, Kyung-Hun;Kim, Jin-Bae;Choi, Ju-Hyeon
    • The Korean Journal of Pesticide Science
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    • v.6 no.4
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    • pp.300-308
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    • 2002
  • This study was conducted to investigate the downward mobility of pesticides using soil colunms and to compare the experimental results with predicted values from Convective mobility test model. Five pesticides including ethoprophos, procymidone, iprobenfos, isoprothiolane, and butachlor were subjected to soil column leaching test for three types of cultivation soils. The concentrations of ethoprophos, iprobenfos, procymidone, isoprothiolane and butachlor leached from soil column of 30 cm depth ranged $0.74{\sim}3.61mg/mL,\;0.36{\sim}1.67mg/L,\;0.16{\sim}0.84mg/L,\;0.16{\sim}0.67mg/L$ and lower than 0.15 mg/L, respectively. Elution volume to reach the peak of ethoprophos, iprobenfos, procymidone, isoprothiolane and butachlor in the leachate ranged $2{\sim}4PV,\;3{\sim}10PV,\;5{\sim}13PV,\;4{\sim}14PV\;and\;19{\sim}61PV$, respectively. Convection times predicted by Convective mobility test model at standard conditions were $9{\sim}18$ days for ethoprophos, $17{\sim}35$ days for iprobenfos, $24{\sim}54$ days for isoprothiolane, $21{\sim}65$ days for procymidone and $105{\sim}279$ days for butachlor. Based on these convection times, ethoprophos was classified as mobile or most mobile, isoprothiolane and procymidone as moderately mobile or mobile and butachlor as slightly mobile. On the same conditions, convection times from the model were coincided with those from soil column test in most of the soil-pesticide combinations applied. Therefore, Convective mobility test model could be applied to predict convection times of pesticides.

The study of binocular function of college students (대학생들의 시기능에 대한 연구)

  • Cho, Young-Rae;Park, Hyun-Ju;Seo, Ji-Keun;Seong, Jeong-Sub;Kim, Jae-Min
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.2
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    • pp.103-110
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    • 2005
  • Binocular vision had a short history in Korea. As there were many near works in these days, the needs about comparative study have been increased. There was related to both refractive error and binocular anomalies, but it is difficult to applying for binocular vision expected findings in itself due to the fact that Korean differ from foreigner. Objects were 100 adults in 18-36 years old ages, The test was Von Gaefe method and used aparatus was phoropter(Shinnippon VT10)and visual chart(Shinnippon CT30). According to interview results was that symptom in near works were headaches 28.0%, blinking 27.3%, red eye 25.1%, eyepain 15.6%, watering 15.3%, itch 12.2%, photophobia 8.5% and eye strain 7.4%. A people who have above ${\pm}0.50$ D refractive error in total objectives (100-male 45/female 55) were classified into ametropia. There was a results such as emmetropia (12.0%), ametropia(88.0%), exophoria(32.0%), esophoria(12.0%). Far negative relative convergence were that in case of high 43.0%, in case of low 7.0%. Far positive relative convergence were that in case of high 15.0%, in case of low 38.0%. Near phoria was exophoria(32.0%), esophoria(12.0%). Near negative relative convergence were that in case of high 23.0%, in case of low 38.0%. Far positive relative convergence were that in case of high 29.0%, in case of low 23.0%. Near negative relative accommodation were that in case of high 10.0%, in case of low 14,0%, Far positive relative convergence were that in case of high 69.0%, in case of low 12.0%. Results were different from expected findings, and especially positive relative accommodation was very high, However, We suggest that the expected findings in Korea for several subjects must study in binocular function.

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Promotional Strategies of Local Drugstores

  • Kim, Seung-Mi;Lee, Sang-Yoon;Kim, Pan-Jin;Kim, Nam-Myun;Youn, Myoung-Kil
    • The Journal of Industrial Distribution & Business
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    • v.1 no.1
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    • pp.5-12
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    • 2010
  • The retail business of drugstore was introduced to Korea for the first time 10 years ago. Since Olive Young introduced a retail store in the name of drugstore in 1999 for the first time in Korea, new distribution channel combining drugstore, cosmetic products and dairy products, etc has been made. At initial stage, the new distribution channel grew up slowly because of low specialty and economic stagnation. However, the three big distribution channels, that is to say, Olive Young (CJ), Watsons (GS) and W Store (Kolon Well Care), etc, were established to produce new distribution system following large-scaled discount stores as well as convenience stores. The purpose of the study is to investigate ways making Korean style drugstore be new retail business in addition to traditional markets, department stores, E-Mart and other general super markets and to examine problems preventing the drugstore from being promoted and to find out solutions. The speciality retailers that is called a category killer attacking department stores as well as marts is expanding market quickly. New consumption trend that gives priority to wellbeing is being expanded in accordance with high level of standards of living life: The drugstore is thought to be new alternative of distribution because it keeps special products. Young ladies who are main customers of drugstores respond to the trend sensitively to have more buying power that is thought to be promising. And, consumers' desire has become concrete and special. This is because consumers want not only convenient shopping but also special shopping system that is current trend. These days, so called Multi-shop and Total shop and other special shops have been recently opened. Special multi-shop has been concentrated on fashion product and miscellaneous goods so far: Health total wellbeing shop shall be popular in accordance with wellbeing trends. Drugstores can play an important role. Drugstores were opened for the first time ten years ago. In particular, Olive Young succeeded in going into the black after making efforts for a long time by many persons. Drugstores could succeed in the business owing to many persons in the past as well as customers who liked drugstores. However, drugstores once lost ways and recorded poor business results. The three drugstores, that is to say, Olive Young, Watsons making efforts to go into the black and W-Store pursuing traditional drugstore shall compete each other and make effort to satisfy customers' desire. In that way, the three drugstores can be assured of present business as well as future business. The consumers' demand trend has become special at sub-division so that drugstores that can satisfy the demand can succeed in the business. Large businesses may be more interested in the 4th generation retail business to produce good income and to have bright future. Drugstore business and market are likely to expand and develop owing to large business' participation in drugstore business. Drugstores expanded shop at Seoul and Gyeonggi-do until middle of 2000. Drugstore business at station sphere in Seoul and Gyeonggi-do that have high ratio of temporary population has low customer loyalty to have limitation on continuous growth. Since 2009, drugstores have opened new shops at local towns: From the year of 2010, drugstores need to establish multiple shop strategy by accelerating business speed and to allow customers to drop in the shop anywhere in the nation and to enter consumers' life deeply, so that they can strengthen business base definitely. Drugstores need to have price competitiveness to have multiple shop opening strategy and to satisfy consumers and to supply high quality services that is future subject to solve. And, Olive Young and Watsons that are Korean style drugstore need to keep system in order and to strengthen substance as Korean style drugstore and to expand marketing, so that they can get business outcome within 5 years that was done 10 years before and they become the 4th generation retail business. The study had difficulties at collecting material from the three drugstore because of poor cooperation. And, the author had great difficulty at collecting statistical material that was made in disorder. Further effort is needed considering such problems.

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The Effect of College students' Perceived Marketing Communication, Value and Consumption Emotion on Store Loyalty in Discount Store (대학생들이 지각하는 종합슈퍼마켓의 마케팅 커뮤니케이션, 가치, 소비감정이 점포충성도에 미치는 영향)

  • Yang, Hoe-Chang;Ju, Yoon-Hwang
    • Journal of Distribution Science
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    • v.10 no.2
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    • pp.19-28
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    • 2012
  • Rapidly growing sales amount and the number of discount stores caused many side effects and sensitive issues in Korea. Because these severe competition due to more expensive cost just like excessive increase in advertising and location selection, and these caused completely ruined small merchants as well as passed on to the consumer. This Study focused on competitiveness of discount store in Korea to the perspective of college students, as explored the relationships between marketing communication and store loyalty. And, examined for two moderating effect, 1) consumers' value separated by hedonic value and utilitarian value between marketing communication and store loyalty, and 2) consumers' value separated by hedonic value and utilitarian value between marketing communication and consumption emotion. Finally, this study examined for mediating effect of consumption emotion between marketing communication and store loyalty. In order to verify the relationship, moderating and mediating effects, data were collected from 130 college students in Whasung, Gyeonggi Province to test theoretical model and its hypotheses. Findings are as followed : First, analysis showed that factors such as advertisement(β =.221, p<.05), publicity(β =.513, p<.01), sales promotion(β =.234, p<.01), word of mouth(β =.627, p<.01) and physical environment(β =.339, p<.01) for marketing communication in the discount store have statistically significant positive effect on store loyalty. But the result of regression analysis for which factors are more impact in marketing communication between store loyalty showed that word of mouth(β =.53, p<.01) is only statistically significant. Second, publicity(β =-.895, p<.05), the sub-dimension of marketing communication shows only statistically significant negative moderating effect on store loyalty. But, the results of the moderating effect of value between marketing communication and consumption emotion verified that utilitarian value show statistically significant, specifically advertisement(β =.294, p<.01), physical environment(β =.418, p<.01), sales promotion(β =.245, p<.01), word of mouth(β =.414, p<.01) and publicity(β =1.137, p<.05), respectively. And hedonic value show statistically significant, specifically advertisement(β =.286, p<.01), physical environment (β =.418, p<.01), sales promotion(β =.236, p<.01) and word of mouth(β =.420, p<.01), respectively. But publicity(β =.145, p=.119) is not statistically significant. Finally, the results verified mediating effect for consumption emotion between all factors for marketing communication and store loyalty showed that factors such as advertisement, publicity, word of mouth and physical environment for marketing communication except sales promotion were statistically significant fully mediated in advertisement, and partially mediated in publicity, word of mouth and physical environment. This testified that the consumption emotion had the most important factor to enhance store loyalty to the perspective of College students. These results can provide important implications and invaluable tips for planning marketing strategies and gaining access to new potential customers. Implications and future research directions are also discussed.

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Occurrence Characteristics of Weed Flora by Regions and Agro-Climatic Zonal in Paddy Fields of Korea (우리나라 지역별 및 농업기후지대별 논잡초 발생상황)

  • Lee, In-Yong;Oh, Young-Ju;Park, Jungsoo;Choi, Jun-Keun;Kim, Eun Jeong;Park, Kee Woong;Cho, Seng-Hyun;Kwon, Oh-Do;Im, Il-Bin;Kim, Sang-Kuk;Seong, Deok-Gyeong;Kim, Chang-Seog;Lee, Jeongran;Seo, Hyun-A;Kim, Whan-Su
    • Weed & Turfgrass Science
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    • v.6 no.1
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    • pp.11-20
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    • 2017
  • Ninety species belonging to 28 families of weeds were identified in Korean rice fields. They were divided by eight provinces and 19 agro-climatic zones to be used as basic data of weed control. Looking at the regional weed occurrence, there were 52 species of 20 families in Gyeonggi, 37 species of 17 families in Gangwon, 41 species of 15 families in Chungbuk, 21 species of 12 families in Chungnam, 24 species of 13 families in Jeonbuk, 54 species of 21 families in Chonnam, 36 species of 20 families in Gyeongbuk, and 32 species of 16 families in Gyeongnam province, respectively. The most dominant family was Poaceae followed by Cyperaceae and Asteraceae. Mostly dominant species were Echinochloa spp., Monochoria vaginalis var. plantaginea, Scirpus juncoides var. hotarui, Eleocharis kuroguwai, and Sagittaria sagittifolia subsp. leucopetala with slight differences among the provinces. Although there were some differences in 18 climate zones from Taebaek sub-highlands to the southern part of the East Coast (except for the Taebaek Highland), the dominant species were Echinochloa spp., Monochoria vaginalis var. plantaginea and Scirpus juncoides var. hotarui. The most dominant family was Cyperaceae followed by Poaceae and Asteraceae. The differences of weed occurrence between provinces and agro-climatic zones were largely influenced by various weather conditions rather than the provinces. The changes in cultivation mode and herbicide use might influence as well.

Physiochemical Properties and Plant Growth of The Hydroponic Substrate Using Waste Wood Chip (양액재배용 목재고형배지의 이화학적 특성과 작물생육 특성)

  • Kwon, Gu-Joong;Yang, Ji-Wook;Park, Hyo-Sub;Cho, Joon-Hyeong;Kim, Dae-Young
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.3
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    • pp.400-409
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    • 2015
  • This study examined the plant growth and development characteristics of leafy vegetables on the hydroponic substrates of waste wood chips, radiate pine chips, and mat type of waste wood chips. The bulk density of waste wood chips and radiata pine chips were $0.2g/cm^3$ and $0.16g/cm^3$, respectively. The moisture retention properties of both the radiata pine chips and waste wood chips were found to be similar but not better than those of the control rock wool and coco peat hydroponic substrates. The moisture retention property of the mat type was found to be the lowest. The chemical analysis of waste wood hydroponic substrates (w/v) was as follows.; The pH was 6.59, electric conductivity was 6.76 dS/m, total nitrogen content was 0.5%, C/N ratio was 113%, phosphorus was 10.1 ppm, potassium was 77 ppm, calcium was 531 ppm, magnesium was 49 ppm, and sodium was 96 ppm. The results from the radiata pine chemical analysis showed that it had a pH of 5.29, electric conductivity of 4.49 dS/m, total nitrogen content of 0.32%, C/N ratio of 180%, phosphorus of 6.4 ppm, potassium of 83 ppm, calcium of 97 ppm, magnesium of 29 ppm, and sodium of 59 ppm. Except for the plants grown in mat type, the developmental characteristics of the plants grown in rock wool and coco peat hydroponic substrates were similar. Based on the results of the experiment, waste wood resources may possibly be used as an organic solid medium in place of the existing rock wool and coco peat medium.

Volcanic stratigraphy and petrology of Cretaceous volcanic rocks in the eastern part of the Euiseong Basin (의성분지 동부에 분포하는 백악기 화산암류의 화산층서와 암석학적 연구)

  • 정종옥;좌용주
    • The Journal of the Petrological Society of Korea
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    • v.9 no.4
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    • pp.238-253
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    • 2000
  • In the eastern part of the Euiseong Basin acidic~intermediate volcanic rocks widely distribute on the Cretaceous sedimentary basement. Coeval granitic rocks and dyke rocks intruded into the volcanic rocks. Volcanic stratigraphy of study area are andesite lava, dacitic lapilli tuff, dacitic flow-banded lava, rhyolitic bedded tuff, rhyolitic massive tuff, dacitic massive lava, rhyolitlc welded tuff occur from the lower to the upper strata. $SiO_2$ content of the volcanic rocks range from 51 to 74 wt.%. With the increase of $SiO_2$, the contents of $TiO_2$, $Al_2$$O_3$, MgO, FeOT MnO, CaO, $P_2$$O_{5}$ decrease but those of $K_2$O increase. The contents of $Na_2$O show dispersive variation. This trend is quite sim-ilar to the major oxide variation in the volcanic rocks from the Yucheon sub-basin. The geochemical natures indicate that the volcanic rocks in the study area are discriminated to the island-arc type high K to medium K calc-alkaline rocks. The compositional variation of the volcanic rocks can be explained by the plagioclase fractionation of the volcanic magmas originated from similar source materials. The volcanic stratigraphy seems to have formed by at least two eruptive sequences of andesitic to rhyolitic and dacitic to rhyolitic magmas which underwent crystallization differentiation.

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