• Title/Summary/Keyword: Sub-division method

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Denaturing Gradient Gel Electrophoresis and Culture-based Analysis of the Bacterial Community in Cheonggukjang, a Korean Traditional Fermented Soybean Food from Gangwon Province (DGGE 방법과 배양법을 이용한 강원지역 전통 발효 청국장에서 미생물의 다양성 분석)

  • Hong, Sung Wook;Lim, In Kyu;Kim, Yong Woo;Shin, Seung-Mee;Chung, Kun Sub
    • Korean Journal of Food Science and Technology
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    • v.45 no.4
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    • pp.515-520
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    • 2013
  • Bacterial communities derived from cheonggukjang and raw rice straw collected from a Mireuksan farm and a Heungup cheonggukjang in Gangwon province were investigated using both culture-based method and denaturing gradient gel electrophoresis (DGGE) analysis. Pure cultures, which were isolated from raw rice straw and cheonggukjang and cultured on tryptic soy agar plates (53-76 colonies per plate), were identified by analysis of 16S rRNA sequences. The traditional culture-based method and analysis of PCR-amplified 16S rRNA by DGGE revealed that for samples collected from the Mireuksan farm, Pantoea agglomerans and Bacillus subtilis were the predominant species in the raw rice-straw and cheonggukjang, respectively. For samples collected from the Heungup cheonggukjang, Bacillus amyloliquefaciens was the predominant species in both raw rice straw and cheonggukjang. Other microorganisms, including members of Pantoea, Bacillus, Enterococcus, Enterobacter, Pseudomonas, Rhodococcus, and Acinetobacter, were also present in the raw rice-straw and cheonggukjang, as were bacteria that could not be cultured.

A Design of LORAN Disciplined Oscillator

  • Hwang, Sang-Wook;Choi, Yun Sub;Yeo, Sang-Rae;Park, Chansik;Yang, Sung-Hoon;Lee, Sang Jeong
    • Journal of Positioning, Navigation, and Timing
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    • v.2 no.1
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    • pp.75-80
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    • 2013
  • This article presents the design of long range navigation (LORAN)-disciplined oscillator (LDO), employing the timing information of the LORAN system, which was developed as a backup system that corrects the vulnerability of the global positioning system (GPS)-based timing information utilization. The LDO designed on the basis of hardware generates a timing source synchronized with reference to the timing information of the LORAN-C receiver. As for the LDO-based timing information measurement, the Kalman filter was applied to estimate the measurement of which variance was minimized so that the stability performance could be improved. The oven-controlled crystal oscillator (OCXO) was employed as the local oscillator of the LDO. The controller was operated by digital proportional-integral-derivative (PID) controlling method. The LDO performance evaluation environment that takes into account the additional secondary factor (ASF) of the LORAN signals allows for the relative ASF observation and data collection using the coordinated universal time (UTC). The collected observation data are used to analyze the effect of ASF on propagation delay. The LDO stability performance was presented by the results of the LDO frequency measurements from which the ASF was excluded.

A Study on Performance Analyses of Korea's Bidding and Contract Systems for Public Construction Projects

  • Beak, Seung-Ho;Kang, Tai-Kyung;Park, Wonyoung;Lee, Yoo-Sub
    • Journal of Construction Engineering and Project Management
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    • v.5 no.3
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    • pp.18-28
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    • 2015
  • Bidding and contract systems are used for public construction projects to select contractors following fair competition principles and to execute national budgets effectively. Many challenges have arisen due to a lack of transparency and fairness and because bidding practices have been luck-based. Few comprehensive or comparative analyses have been conducted on the performance and limitations of bidding and contract systems, and empirical analyses designed to improve policies on and the practice of such systems are lacking. This study empirically analyzed current bidding and contract systems to seek ways of improving them. The study proposes several alternatives to resolve the problems with and irrationalities of the current system: 1) improving bidding and selection systems by changing them from a luck-based price competition into a technical merit- and value-based competition; 2) improving the assessment criteria to meet the current market level of bid and winning prices; 3) adjusting contractual responsibilities and sharing structures to meet the current trend; and 4) strengthening the competitiveness and expanding the social responsibility-based procurement systems of construction companies.

A novel approach for the design of multi-class reentrant manufacturing systems

  • Yoo, Dong-Joon;Jung, Jae-Hak;Lee, In-Beum;Lee, Euy-Soo;Yi, Gyeong-beom
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.710-715
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    • 2004
  • The design problem of manufacturing system is addressed, adopting the closed queueing network model with multiple loops and re-entrant flows. The entire design problem is divided into two hierarchical sub-problems of (1) determining the station configuration and (2) optimizing the lot constitution; then they are tackled by neighbor search algorithm (NSA) and greedy mean value analysis (GMVA), respectively. Unlike the conventional MVA concerning multi-class closed queueing networks, the GMVA doesn't stick to a fixed lot proportion; rather it tries to find the optimal balance. The NSA, on the other hand, improves the object function value by altering the station configuration successively with its superior neighbor. The moderate time complexity, presented in big-${o}$ notation, enables us to apply the method even to the large-size practical cases, and the CPU time of an enlarged problem can be approximated by the same equation. The validity of our analytic approach is backed up by simulation studies with a widespread simulation package.

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A Study of Weldability for Pure Titanium by Nd:YAG Laser(I) - Weld Properties with Shield Conditions - (순티타늄판의 Nd:YAG 레이저 용접성에 관한 연구(I) - 실드 조건에 따른 용접특성 -)

  • Kim, Jong-Do;Kwak, Myung-Sub;Kim, Chang-Soo
    • Journal of Welding and Joining
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    • v.27 no.5
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    • pp.55-61
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    • 2009
  • Pure titanium and its alloys have good formability, excellent corrosion resistance and high strength to weight ratios. Therefore, it has been using to heat exchangers, offshore plants, sports equipments, and etc. As broad as its application fields, it also increases welding locations. Conventional GTAW and GMAW are very popular welding methods of titanium, but it has a high heat input and wide HAZ. It has a possibility of inducing Stress Corrosion Cracking. So, laser welding method has been using to get reliable welds by reducing heat input. Weld beads change its color to silver, gold, brown, blue, and gray by shied conditions. And the closer to gray, the more oxidize, nitride and embrittlement. The most effective atom to embrittlement was nitrogen. And shield gas flow was not so effective over the constant flow rates. In this study, weld properties of the pure titanium were investigated by pulsed & CW Nd:YAG lasers and evaluated by various shield conditions. And It is observed that nitrogen is more effective to oxidation and embrittlement of titanium compared with oxygen by oxygen and nitrogen quantitative analysis.

DESIGN AND DEVELOPMENT OF IRRI POWER TILLER-DRIVEN DRILLING RIG FOR SHALLOW TUBEWELLS

  • Salazar, G.C.;Quick, G.R.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.227-238
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    • 1993
  • IRRI Engineering Division has developed a well drilling rig attachment that matched power tiller or hand tractor. It was designed in response to the growing demand for ground water utilization for small-scale irrigation, especially in drought-sticken and rained farms in Asian countries. The power tiller-driven rig can drill 30 meters of 100mm well in an unconsolidated formation in one day and can be rapidly converted from rotary to jetting or to the percussion method of drilling to suit different soil and rock formation. In addition, the power tiller can be quickly installed or removed from the rig frame and can be used for transporting the rig to other sites. The rig can be dismantled into smaller sub-assemblies for carrying by hand into less accessible areas. One manufacturer in Central luzon Philippines has started to produce the rig for well drillers in Central Luzon. The Department of Agriculture in the Philippines have procured thirty three(33) units of these machines f r their Shallow Tubewell program.

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The Error Analysis of measuring wind speed on Met Mast Shading Effect (기상탑 차폐 영향에 따른 측정 풍속의 오차 분석)

  • Ko, Suk-Whan;Jang, Moon-Seok;Lee, Yoon-Sub
    • Journal of the Korean Solar Energy Society
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    • v.31 no.3
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    • pp.1-7
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    • 2011
  • In the performance test for wind turbines of medium and large, The reference met-mast should be installed for measurement reference wind speed as IEC 61400-12-1 standards and design of booms for mounted an anemometer must be considered exactly. Boom-mounted cup anemometer are influenced by flow distortion of the mast and the boom. Therefore design of booms must be important so that flow distortion due to booms should be kept below 0.5%. But, in some cases at size of met-mast structure, the distance of boom from mast is longer then measurement of wind speed is impossible because of oscillation of boom-mounted anemometer. In this paper, We measured a wind speed at several point from mast and boom and we analyzed the error of wind speed at each point of measurement. Also, we will suggest a correction method using the data curve fitting about errors of wind speed between each point of mounted anemometer.

Design for Sequential Control System Using Petri Nets with Hierarchical Expression(I) - Division of Petri Nets Based on SFC (페트리네트의 계층화를 통한 시퀀스제어계의 설계(I) - SFC에 근거한 페트리네트의 분할)

  • Jeong, Seok-Kwon;Yang, Joo-Ho
    • Journal of Ocean Engineering and Technology
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    • v.13 no.3B
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    • pp.106-115
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    • 1999
  • Modeling a discrete event system such as a sequential control system is difficult compared with a continuous system. Petri nets have been introduced as an analyzing and design tool for the discrete systems. One of the problems in its applications is that the model can not be analyzed easily in the case of large scale or complicated systems because of increase of the number of components of the system. To overcome this problem, some methods for dividing or reducing Petri nets have been suggested. In this paper, an approach for a hierarchical expression of Petri nets based on Sequential Function Chart(SFC) is proposed. A measuring tank system will be described as a typical kind of discrete systems. The system is modeled by sub Petri nets based on SFC in order to analyze and visualize efficiently about the dynamic behaviors of the system. Some numerical simulations using state equations are performed to prove the validity of the proposed method.

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Energy Efficiency Maximization for Energy Harvesting Bidirectional Cooperative Sensor Networks with AF Mode

  • Xu, Siyang;Song, Xin;Xia, Lin;Xie, Zhigang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.6
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    • pp.2686-2708
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    • 2020
  • This paper investigates the energy efficiency of energy harvesting (EH) bidirectional cooperative sensor networks, in which the considered system model enables the uplink information transmission from the sensor (SN) to access point (AP) and the energy supply for the amplify-and-forward (AF) relay and SN using power-splitting (PS) or time-switching (TS) protocol. Considering the minimum EH activation constraint and quality of service (QoS) requirement, energy efficiency is maximized by jointly optimizing the resource division ratio and transmission power. To cope with the non-convexity of the optimizations, we propose the low complexity iterative algorithm based on fractional programming and alternative search method (FAS). The key idea of the proposed algorithm first transforms the objective function into the parameterized polynomial subtractive form. Then we decompose the optimization into two convex sub-problems, which can be solved by conventional convex programming. Simulation results validate that the proposed schemes have better output performance and the iterative algorithm has a fast convergence rate.

The 'Good Nursing' Experienced by Hemodialysis Patients (혈액투석 환자가 경험한 '좋은 간호')

  • Yeom, Eun Yi;Kim, Miyoung
    • Korean Journal of Adult Nursing
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    • v.26 no.3
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    • pp.275-286
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    • 2014
  • Purpose: The purpose of this study was to explore the meaning and essence of good nursing from the perspective of patients undergoing hemodialysis. Methods: The data were collected through in-depth interviews of five hemodialysis patients who have been dialyzed for one year or longer. The data analysis was conducted using the Giorgi's phenomenological method. Results: The findings included six constituents and 13 sub-constituents. The six constituents were 'prompt and skillful response to mitigate distress', 'careful attention to provide relief', 'individualized education to promote self-management', 'an intimate relationship to regain isolated ego', 'warmhearted support to encourage strength to live in a exhausting life', and 'providing of comfortable environment to maintain stability'. Conclusion: From the perspective of the hemodialysis patients, the concept of good nursing should stem from the integration of nurses' scientific knowledge, expert skills, and moral sensitivity. The finding suggests that strategies to develop nursing interventions for hemodialysis patients will be required on the basis of understanding of their sociocultural contexts and familism.