• Parasites, Hosts and Diseases
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• 제59권4호
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• pp.329-339
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• 2021

Ion channels are important targets of anthelmintic agents. In this study, we identified 3 types of ion channels in Ascaris suum tissue incorporated into planar lipid bilayers using an electrophysiological technique. The most frequent channel was a large-conductance cation channel (209 pS), which accounted for 64.5% of channels incorporated (n=60). Its open-state probability (P_o) was ~0.3 in the voltage range of -60~+60 mV. A substate was observed at 55% of the main-state. The permeability ratio of Cl^- to K⁺ (P_Cl/P_K) was ~0.5 and P_Na/P_K was 0.81 in both states. Another type of cation channel was recorded in 7.5% of channels incorporated (n=7) and discriminated from the large-conductance cation channel by its smaller conductance (55.3 pS). Its Po was low at all voltages tested (~0.1). The third type was an anion channel recorded in 27.9% of channels incorporated (n=26). Its conductance was 39.0 pS and P_Cl/P_K was 8.6±0.8. P_o was ~1.0 at all tested potentials. In summary, we identified 2 types of cation and 1 type of anion channels in Ascaris suum. Gating of these channels did not much vary with voltage and their ionic selectivity is rather low. Their molecular nature, functions, and potentials as anthelmintic drug targets remain to be studied further.

• ALGAE
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• 제36권4호
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• 한국응용곤충학회지
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• 제61권1호
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• pp.117-128
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• 2022

본 연구는 피레스로이드계 살충제인 퍼메트린이 Heliothis virescens의 중추신경세포의 나트륨채널에 어떻게 작용하는 가를 전기생리학적으로 관찰하였다. 퍼메트린은 나트륨채널의 꼬리전류(I_Na-tail)를 지속적으로 증가시켰으며 이러한 비정상적인 나트륨 전류증가가 나방류의 신경계에 과도한 흥분을 일으겨 살충작용을 하는 것으로 생각된다. 이러한 살충작용은 전갈독과 함께 사용했을때 약 8배의 증가가 있었음을 확인하였다. 전갈독이 살충제의 독성을 강화하는 분자생리학적 기전연구가 계속되면 해충방제에 많은 기여를 할 것으로 생각된다.

• BMB Reports
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• 제54권6호
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• pp.311-316
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• 2021

Ethanol often causes critical health problems by altering the neuronal activities of the central and peripheral nerve systems. One of the cellular targets of ethanol is the plasma membrane proteins including ion channels and receptors. Recently, we reported that ethanol elevates membrane excitability in sympathetic neurons by inhibiting Kv7.2/7.3 channels in a cell type-specific manner. Even though our studies revealed that the inhibitory effects of ethanol on the Kv7.2/7.3 channel was diminished by the increase of plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂), the molecular mechanism of ethanol on Kv7.2/7.3 channel inhibition remains unclear. By investigating the kinetics of Kv7.2/7.3 current in high K⁺ solution, we found that ethanol inhibited Kv7.2/7.3 channels through a mechanism distinct from that of tetraethylammonium (TEA) which enters into the pore and blocks the gate of the channels. Using a non-stationary noise analysis (NSNA), we demonstrated that the inhibitory effect of ethanol is the result of reduction of open probability (P_O) of the Kv7.2/7.3 channel, but not of a single channel current (i) or channel number (N). Finally, ethanol selectively facilitated the kinetics of Kv7.2 current suppression by voltage-sensing phosphatase (VSP)-induced PI(4,5)P₂ depletion, while it slowed down Kv7.2 current recovery from the VSP-induced inhibition. Together our results suggest that ethanol regulates neuronal activity through the reduction of open probability and PI(4,5)P₂ sensitivity of Kv7.2/7.3 channels.

• The Korean Journal of Physiology and Pharmacology
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• 제27권2호
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• pp.187-196
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• 2023

Transient receptor potential canonical (TRPC) channels are non-selective calcium-permeable cation channels. It is suggested that TRPC4β is regulated by phospholipase C (PLC) signaling and is especially maintained by phosphatidylinositol 4,5-bisphosphate (PIP₂). In this study, we present the regulation mechanism of the TRPC4 channel with PIP₂ hydrolysis which is mediated by a channel-bound PLCδ1 but not by the G_qPCR signaling pathway. Our electrophysiological recordings demonstrate that the Ca²⁺ via an open TRPC4 channel activates PLCδ1 in the physiological range, and it causes the decrease of current amplitude. The existence of PLCδ1 accelerated PIP₂ depletion when the channel was activated by an agonist. Interestingly, PLCδ1 mutants which have lost the ability to regulate PIP₂ level failed to reduce the TRPC4 current amplitude. Our results demonstrate that TRPC4 self-regulates its activity by allowing Ca²⁺ ions into the cell and promoting the PIP₂ hydrolyzing activity of PLCδ1.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -1
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• pp.405-408
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• 2002

Because of superior division among sub-channels, DWT MC-CDMA is less interference with neighborhood channel. In our newly proposed multi-carrier CDMA data transmission method, lIR CMFB filter banks sharply divide sub-bands and reduces spectral overlaps among neighboring sub-channels. In experiment result, proposed filter is better ability of sub-band division than FIR filter. Since the proposed cosine modulated filter banks lIR filter having wavelet property is designed to response sharp transition band, the ISI and ICI between neighboring sub-channels can be effectively reduced. And robustness about narrow band interference of CMFB IIR MC-CDMA almost same FIR DWT MC-CDMA.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.237-239
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• 2007

In the paper, we analyze the performances of random access channels in multi-service multi-user OFDMA systems. The resource of the random access channels in OFDMA systems is the nubmer of available sub-channels and PN-codes. For given available sub-channels and PN-codes. we analyze the performances of the random access channels of OFDMA systems according to three resource allocation methods (resource full sharing, resource partial sharing, resource partition) in tenus of the access success probability, the blocking probability, the access delay and the throughput of each service class. Further, we find the feasible region of the access probability of each service class in which the allowable minimum access success probability, the allowable maximum blocking probability and the allowable maximum access delay are satisfied. The results also can be utilized to find proper region of the access probabilities of each service class for differentiated quality of service(QoS)s, and for the system operations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권6호
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• pp.2649-2669
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• 2020

In this paper, we analyze the secrecy performance of single-input multiple-output (SIMO) relay systems over κ-μ shadowed fading channels. Based on considering relay model employing decode-and-forward (DF) protocol, two security evaluation metrics, namely, secure outage probability (SOP) and probability of strictly positive secrecy capacity (SPSC) are studied, for which closed-form analytical expressions are derived. In addition, Monte Carlo results prove the validity of the theoretical derivation. The simulation results confirm that the factors that enhance the security include large ratio of (μ_D, μ_E), (m_D, m_E), (L_D, L_E) and small ratio of (k_D, k_E) under the high signal-to-noise ratio regime.

• The Korean Journal of Physiology and Pharmacology
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• 제24권6호
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• pp.545-553
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• 2020

Aripiprazole is a quinolinone derivative approved as an atypical antipsychotic drug for the treatment of schizophrenia and bipolar disorder. It acts as with partial agonist activities at the dopamine D2 receptors. Although it is known to be relatively safe for patients with cardiac ailments, less is known about the effect of aripiprazole on voltage-gated ion channels such as transient A-type K⁺ channels, which are important for the repolarization of cardiac and neuronal action potentials. Here, we investigated the effects of aripiprazole on Kv1.4 currents expressed in HEK293 cells using a whole-cell patch-clamp technique. Aripiprazole blocked Kv1.4 channels in a concentration-dependent manner with an IC₅₀ value of 4.4 μM and a Hill coefficient of 2.5. Aripiprazole also accelerated the activation (time-to-peak) and inactivation kinetics. Aripiprazole induced a voltage-dependent (δ = 0.17) inhibition, which was use-dependent with successive pulses on Kv1.4 currents without altering the time course of recovery from inactivation. Dehydroaripiprazole, an active metabolite of aripiprazole, inhibited Kv1.4 with an IC₅₀ value of 6.3 μM (p < 0.05 compared with aripiprazole) with a Hill coefficient of 2.0. Furthermore, aripiprazole inhibited Kv4.3 currents to a similar extent in a concentration-dependent manner with an IC₅₀ value of 4.9 μM and a Hill coefficient of 2.3. Thus, our results indicate that aripiprazole blocked Kv1.4 by preferentially binding to the open state of the channels.

• 한국통신학회논문지
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• 제31권7C호
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• pp.680-690
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• 2006

본 논문에서는 V-BLAST(Vertical-Bell labs LAyered Space-Time) 검출기를 사용하는 MIMO-OFDM(Multi Input Multi Output-Orthogonal Frequency Division Multiplexing) 시스템에서, 그룹화된 부채널 기반의 간단한 형태의 비트 할당 기법인 SBA-GS(Simplified Bit Allocation based on Grouped Sub-channels)를 제안한다. 2차원 Water Pouring 원리에 기반하여 MIMO-OFDM 시스템 수신단에서 각 부채널별 비트수 및 전력을 결정하여 궤환하는 일반적인 ABPA(Adaptive Bit and Power Allocation)은 비트오율 측면에서 최적의 성능을 보이지만, 수신단에서 송신단으로의 많은 양의 궤환 정보를 필요로 하고 시스템이 복잡하다는 단점을 가진다. 이러한 단점을 보완하기 위해, 상태가 우수한 각 부채널에 동일한 수의 비트를 할당하여 송신단에서 수신단으로의 궤환 정보량과 시스템 복잡도를 감소시킨 SBA가 제안된 바 있다. 본 논문에서 제안하는 SBA-GS 는 부채널들을 그룹화한 뒤 각 그룹별 부채널들의 펑균 신호대잡음비를 구하여 동일한 변조 방식을 적용하는 SBA를 수행한다. 다양한 차세대 이동통신 채널 환경에서의 모의실험 결과, 지연 확산이 작은 다중 경로 채널의 경우에서는 궤환 정보량을 크게 감소하면서도 SBA와 유사한 결과를 얻을 수 있었 으며, 지연확산이 큰 채널 환경에서는 부채널 그룹화에 따른 BER 성능 열화가 상대적으로 증가하였지만 궤환 정보량 감소와의 절충 관계를 감안할 때 실제 시스템 구현시 고려될 수 있는 우수한 결과를 보임을 확인하였다.