• Journal of Ginseng Research
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• 제45권2호
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• pp.211-217
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• 2021

The treatments of nervous system diseases (NSDs) have long been difficult issues for researchers because of their complexity of pathogenesis. With the advent of aging society, searching for effective treatments of NSDs has become a hot topic. Ginseng polysaccharides (GP), as the main biologically active substance in ginseng, has various biological properties in immune-regulation, anti-oxidant, anti-inflammation and etc. Considering the association between the effects of GP and the pathogenesis of neurological disorders, many related experiments have been conducted in recent years. In this paper, we reviewed previous studies about the effects and mechanisms of GP on diseases related to nervous system. We found GP play an ameliorative role on NSDs through the regulation of immune system, inflammatory response, oxidative damage and signaling pathway. Structure-activity relationship was also discussed and summarized. In addition, we provided new insights into GP as promising neuroprotective agent for its further development and utilization.

• 한국산업정보학회논문지
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• 제27권3호
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• pp.145-164
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• 2022

본 논문에서는 중소기업이 성공적인 산학협력을 위하여 필요한 요인을 확인하였다. 기업의 산학협력준비역량은 TOE(Technology, Organization, Environment) 구조의 구조를 고려하였으며, 산학협력준비역량과 기업성과 간의 매개효과로 개방형혁신협업과 회복탄력성을 활용하였다. 본 연구는 산학협력 경험이 있는 중소기업 204개를 대상으로 구조화된 설문을 통해 모형을 검증하였으며, 이를 통해 산학협력의 준비역량이 기업성과에 유의한 영향을 미치며 설정한 각 변수의 매개효과를 확인하였다. 결과적으로 산학협력으로 인한 성과를 얻기 위해서는 기업에서 산학협력을 수용하고자 하는 다각적인 노력 여부가 중요함을 확인하였다.

• 국제학술발표논문집
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• The 3th International Conference on Construction Engineering and Project Management
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• pp.1288-1293
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• 2009

Performing 'Mega Project' in which various stakeholders are involved is difficult to control due to the respective conflicting objectives. Urban regeneration which is being challenged issue in various ways can be defined as the mega project. Since the mega project like urban regeneration a long-continued project and includes a lot of participants whose benefits conflict, cooperation among stakeholders is essential. Moreover, as the project goes, diverse decision making situations which require collaboration happen, and if proper decision making cannot be given at that time, the whole project would have critical effects. Also, since each activity concerns numerous stakeholders, prompt decision making is difficult in the mega project. Therefore, the objective of this study is to support stakeholder's cooperative decision making in order for conducting successful project. For the purpose of the paper, the concept of cooperation in mega project is redefined and a cooperative decision making framework is suggested. Also, the term of governance, which means cooperative mechanism based on the mutual trust, information sharing and partnership, is applied to the newly defined cooperation. The suggested cooperative decision making framework contains the structure of stakeholders in urban regeneration project which enables participants to understand the relationship among bodies and checklists for supporting decision making appropriately. The application of the framework proposed in this research is expected to contribute subjects' suitable decision making by providing proper information in a timely manner.

• Safety and Health at Work
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• 제15권3호
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• pp.368-372
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• 2024

Occupational use of cleaning products can cause asthma in healthcare workers but the cleaning agents responsible are not yet known. This study aimed to identify respiratory and other hazards in cleaning products on the National Health Service (NHS) supply chain online catalogue and used in the NHS. Information on cleaning products, their composition, and H-statements that identified hazard characteristics of chemical substances in them was obtained from chemical safety data sheets (SDSs). Furthermore, a quantitative structure-activity relationship model and a published asthmagen list were used to identify potential additional respiratory hazards. 473 cleaning products and 229 substances were identified. SDSs reported only 4 respiratory sensitizers but an additional 51 were suggested by the other 2 methods. In contrast, 25 respiratory irritants were identified using SDSs and only one from the asthmagen list. This comprehensive overview of cleaning agents' hazards has potential use in future risk assessment and epidemiological studies.

• 무역상무연구
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• 제38권
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• pp.377-408
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• 2008

Globalization of R&D activities of multinational firms is becoming important in compared with the past, because of increasing globalization of world economy. So, every country tries to attract foreign investment including R&D activities. In this paper, effects of several factors, which determine the scale of foreign subsidiary's R&D activities in local market, are analyzed. Through the review of previous literature, I found that three groups of determinant factors. The first one is the factor which is related to characteristics of local market, such as competitive situation of local market, accessability to research institutions, etc. The second group is the factor which is related to relationship between foreign subsidiary and its mother company. The dominant factors of this group are operation strategy of foreign subsidiary, competitiveness of mother firm, and equity structure of foreign subsidiary. Finally the characteristics of industry affect to the scale of foreign subsidiary's R&D activities. Among these factors, 6 factors are chosen to investigate empirically. For the empirical investigation, 107 foreign subsidiaries among KOITA(Korea Industrial Technology Association)'s list are chosen. Each subsidiary has more than 50% of foreign equity and implement R&D activities in Korea. The results show that sales volume of foreign subsidiary is dominant influencing factor in determining scale of R&D activities in Korean market. And also I found that currently established firms are implementing more R&D activities than old established firms. This is quite interesting finding. However, R&D activities of foreign subsidiary are not vitalized in Korean market. It probably means that Korean government and Korean business partners have to make effort to enhance foreign firms/ R&D activities, because it could contribute technological and economic development of Korean market.

• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.78-84
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• 2008

EGFR has been intensively investigated as a target to block the signal transduction pathway which stimulates cancer growth and metastasis. Studies about structure-activity relationship for tricyclic azepine derivatives were performed with topomer-CoMFA. The derived topomer-CoMFA model with steric and electrostatic field parameters based on fragment units gave reasonable statistics ($q^2$=0.561, $r^2$=0.679). The model explains why a halogen atom at the meta position of aniline is important to increases inhibitory activity. This comes from an electrostatically negative groups are favored near this region. The model also shows that there are sterically favored regions around methoxy group extended from oxazepine derivatives. The findings about steric and electrostatic effects can be utilized for designing new inhibitors.

• 한국지역지리학회지
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• 제9권4호
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• pp.534-545
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• 2003

한국의 화강암 풍화층은 한냉하고도 습윤한 환경에서 사용하는 결빙과 융해에 의한 특이한 구조들이 잘 발달되어 있다. 이들의 구조들은 일반적인 풍화가 진전된 상황에서만 발견된다. 조사지역인 대관령의 풍화층은 두껍지는 않지만 결빙된 특색이 현미경관찰에서 파쇄되지 않은 채로 보존되어 있다. 특히 조립의 모래알에는 미립의 운모류와 실트 중심의 물질들이 압축되어 치밀하게 덮여 있고 풍화층 상층부에 미립물질이 더욱 많아지고 있다. 결빙에 의한 이동현상도 뚜렷하여 현미경하의 토립들은 미약하게 원마되어 공극과 더불어 화석화되었다. 풍화단면층에서는 사면경사를 따라 커브를 나타낸 적색의 band현상도 관찰되었다. 본 연구결과 풍화층은 냉각과 결빙정도가 오늘날과 달랐던 한냉습윤한 환경이 시차를 두고 존재했음을 시사한 고환경 즉 주빙하 조건에서 형성되었고 동시에 결빙포행이 이루어졌다고 추정된다.

• BMB Reports
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• 제40권3호
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• pp.419-425
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• 2007

In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2000년도 춘계학술대회
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• pp.10-14
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• 2000

The cycloooxygenase enzymes catalyze the oxidative conversion of arachidonic acid into prostag1andin H$_2$Which mediates both benificial and pathological effects. The COX-1 is constitutively expressed in most tissues and in blood platelets wherease the expression of COX-2 isoform is induced in response to inflmmatory stimuli such as cyctokynes. Thus the identification of a novel COX-2 selective inhibitor should offer excellent antiinflammatory activity with minimal side effects such as gastrointestinal toxicity. Recently, a group of structurally unique and biologically active pimarane diterpenoids has been isolated from indigenous Korean medicinal plants. These new diterpenoids turned out to be potential analgesic and antiinflammatory agent due to their potent inhibitory activities of prostaglandin synthesis. We have also found that the inhibition of PGE$_2$synthesis is attributed to the potent COX inhibition by pimarane diterpenoid in arachidonic acid cascade. In conjunction with development of new analgesic and nonsteroidal antiinflammatory agent, a series of works on these diterpenoids have been extensively carried out in our laboratories. These efforts involve the structure-activity relationship of pimaradienoic acid, molecular modelings and COX inibitory activities as well as actiinflammatory effects of its structural analogues. In addition, the total syntheses of the new natural pimarane diterpenoids, their stereoisomers and other structural variants were intensively investigated.

• BMB Reports
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• 제41권5호
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• pp.369-375
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• 2008

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a dose-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL.