• Microbiology and Biotechnology Letters
• /
• v.29 no.2
• /
• pp.72-77
• /
• 2001

Expression of f3 ~agarase Gene and Catabolite Repression in Escherichia coli by the Promoter of Alginate Lyase Gene Isolated from Marine Pseudomonas sp. Jin, Cheal~Ho, J~Hyeon Park, Jeong-Hyun Han, YoonM Hyeok Chae, Jong~Hee Lee, Jung-Kee Lee!, and In-800 Kong*. Faculty of Food Science and Biotechnology, Pukyong National UniversitYt Pusan 608-737, Korea, llnBioNet Co. 1690-3 Taejon 306-230, Korea - Promoter is a key factor for expression of the recombinant protein. There are many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter ~md association between the promoter mutants, f3 agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids ofthe alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using peR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of f3 -agarase productivity.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.13 no.4
• /
• pp.79-85
• /
• 2007

Many countries foster the tour and leisure industries as a national strategic industry of the 21st century. The tour and leisure industry is expected to be reorganized from the land-based industry to the ocean-based industry as the GNP in a nation is over USD $20,000{\sim}30,000$ dollars. In Korea, since we adopt a five-day week, the total working hours for a week is shortened. As the result, leisure activities in family, such as experience tour, ecological tour, travel, and overnight tour, are expected to be increased. Leisure and sports tours, which link the tour and sports to rafting, hang gliding, and marine leisure, have been also increased over 21.6%. A number of small and big islands near Ho-Nam coastal areas play an important role as natural breakwater. Thus, the ports in Ho-Nam area is safely protected from physical effects by ocean waves and is maintained without constructing a breakwater. In this paper, we analyses the required conditions as the marine leisure base in the southwest sea area which has the natural advantages for marine leisure. We also discuss how to activate the yacht business on Ho-Nam coast area. And then we analyse the present situations and obstacles of a domestic marine leisure. We also suggest the plan to build up echo-marine leisure infrastructure for industrial growth of marine leisure in Ho-Nam area.

• Wind and Structures
• /
• v.23 no.6
• /
• pp.559-575
• /
• 2016

In order to investigate the influence of different blade positions on aerodynamic load and wind loads and load-effects of large scale wind turbine tower under the halt state, we take a certain 3 MW large scale horizontal axis three-blade wind turbine as the example for analysis. First of all, numerical simulation was conducted for wind turbine flow field and aerodynamic characteristics under different halt states (8 calculating conditions in total) based on LES (large eddy simulation) method. The influence of different halt states on the average and fluctuating wind pressure coefficients of turbine tower surface, total lift force and resistance coefficient, circular flow and wake flow characteristics was compared and analysed. Then on this basis, the time-domain analysis of wind loads and load-effects was performed for the wind turbine tower structure under different halt states by making use of the finite element method. The main conclusions of this paper are as follows: The halt positions of wind blade could have a big impact on tower circular flow and aerodynamic distribution, in which Condition 5 is the most unfavourable while Condition 1 is the most beneficial condition. The wind loads and load-effects of disturbed region of tower is obviously affected by different halt positions of wind blades, especially the large fluctuating displacement mean square deviation at both windward and leeward sides, among which the maximum response occurs in $350^{\circ}$ to the tower top under Condition 8; the maximum bending moment of tower bottom occurs in $330^{\circ}$ under Condition 2. The extreme displacement of blade top all exceeds 2.5 m under Condition 5, and the maximum value of windward displacement response for the tip of Blade 3 under Condition 8 could reach 3.35 m. All these results indicate that the influence of halt positions of different blades should be taken into consideration carefully when making wind-resistance design for large scale wind turbine tower.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.17 no.3
• /
• pp.295-303
• /
• 2015

This paper presents the study of leakage effect due to multi-cell inflater of rapid protection system to protect the possibilities of tunnel damages by flooding threats and unusual leakage to be occurred during and after subsea tunnel construction. Particularly, this protect system should be necessary in subsea tunnel. This research concentrates the physical model tests due to several multi-cell inflater to study protection capacity of leakage between the inflater and tunnel liner. A 27:1 small scale model are used in the model tests. The leakage rate, water pressure and axial displacement of inflater are measured during the model tests. According to the results, the minium leakage rate clearly shows in the case of two-cell inflater compared with in other cases. It is concluded that the results of this research will be very useful to understand the fundamental information of inflater structure design and development the technology of tunnel protection structures in the future.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.14 no.4
• /
• pp.293-308
• /
• 2012

The shear behavior at the particle/surface interface such as rock joint can determine the mechanical behavior of whole structure. Therefore, a fundamental understanding of the mechanisms governing its behavior and accurately estimation of the interface strength is essential. In this paper, PFC, a numerical analysis program of discrete element method was used to investigate the effects of the surface roughness on interface strength. The surface roughness was characterized by smooth, intermediate, and rough surface, respectively. In order to investigate the effects of particle shape and crushing on particle/surface interface behavior, one ball, clump, and cluster models were created and their results were compared. The shape of particle was characterized by circle, triangle, square, and rectangle, respectively. The results showed that as the surface roughness increases, interface strength and friction angle increase and the void ratio increases. The one ball model with smooth surface shows lower interface strength and friction angle than the clump model with irregular surface. In addition, a cluster model has less interface strength and friction angle than the clump model. The failure envelope of the cluster model shows non-linear characteristic. From these findings, it is verified that the surface roughness and particle shape effect on the particle/surface interface shear behavior.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.14 no.4
• /
• pp.337-356
• /
• 2012

Three-dimensional (3D) numerical analyses have been performed to study the behaviour of single piles and grouped piles to adjacent tunnelling in the lateral direction of the pile. In the numerical analyses, the interaction between the tunnel, the pile and the soil next to the piles and shear transfer mechanism have been analysed allowing soil slip at the pile-soil interface by using interface elements. The study includes the shear stresses at the soil next to the pile, the axial force distributions on the pile and the pile settlement. It has been found that existing elastic solutions may not accurately estimate the pile behaviour since several key issues are excluded. Due to changes in the shear transfer between the pile and the soil next to the pile with tunnel advancement, the shear stresses and axial force distributions along the pile change drastically. Downward shear stress develops above the tunnel springline while upward shear stress is mobilised below the tunnel springline, resulting in a compressive force on the pile. In addition, mobilisation of shear strength at the pile-soil interface was found to be a key factor governing pile-soil-tunnelling interaction. It has been found that grouped piles are less influenced by the tunnelling than the single pile in terms of the axial pile forces. The reduction of apparent allowable pile capacity due to pile settlement resulted from the tunnelling seemed to be insignificant.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.14 no.4
• /
• pp.321-336
• /
• 2012

The shear behavior at the particle/surface interface such as rock joint can determine the mechanical behavior of whole structure. Therefore, a fundamental understanding of the mechanisms governing its behavior and accurately estimation of the interface strength is essential. In this paper, PFC, a numerical analysis program of discrete element method was used to investigate the effects of the surface roughness crushing on interface strength. The surface roughness was characterized by smooth, intermediate, and rough surface, respectively. Particle shape was classified into one ball model of circular shape and 3 ball model of triangular shape. The surface shape was modelled by wall model of non-crushing surface and ball model of crushing surface. The results showed that as the bonding strength of ball model decreases, lower interface strength is induced. After the surface roughness crushing was occurred, the interface strength tended to converge and higher bonding strength induced lower surface roughness crushing. Higher friction angle was induced in wall model and higher surface roughness induced the higher friction angle. From these findings, it is verified that the surface roughness and surface roughness crushing effect on the particle/surface interface shear behavior.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.15 no.6
• /
• pp.599-612
• /
• 2013

This study aims to investigate an economical efficiency of two excavation methods with respect to the room-and-pillar method for the underground space and conventional excavation method, i.e. 2-arch tunnelling method. For feasibility study, an excavation cost for both room-and-pillar method and 2-arch tunnelling method was estimated when the same space in operation was required. It was assumed that properties of reinforcements and rock were adopted from literatures. However, an excavation shape of the room-and-pillar method was assumed not to be the rectangular shape which is a general type in the room-and-pillar method but to be an arch shape in order to compare with the conventional excavation method (2-arch tunnelling) and to achieve the maximum bearing capacity of the structure during excavation. Consequently, the wider space in use or required and the better condition of rock we assumed, the more economical advantage we have in the room-and-pillar method than the 2-arch tunnelling method.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.15 no.3
• /
• pp.289-300
• /
• 2013

This study numerically considered the characteristic of smoke movement and the effect of hot smoke gas on tunnel wall surface temperature during road tunnel fire under boundary condition of fire growth curve that is applied to fire analysis in road tunnels. The maximum heat release rate were 20 MW and 100 MW and tunnel air velocities were 2.5 m/s and velocity induced by thermal buoyancy respectively, also the cooling effect of tunnel wall was considered. As results, when tunnel air velocity was constant at 2.5 m/s during tunnel fire, due to the cooling effect of tunnel wall, the smoke layer was rapidly descent after some distance and it flowed the same patterns at the downstream. When heat release rate was 100 MW (and jet fan was not installed), the maximum temperature of tunnel wall surface has risen up to $615^{\circ}C$. The heat transfer coefficient of tunnel wall surface was varied from 13 to $23W/m^2^{\circ}C$ approximately.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.20 no.2
• /
• pp.331-346
• /
• 2018

In the tunnel of domestic high - speed railway, the main fire - fighting facility, fire - extinguishing passageway, is installed. However, due to the high pressure of the high - speed train, frequent breakage and maintenance are caused by strong shock and long - term vibration. In order to solve these problems, it is necessary to improve the fire door, but in Korea, it is installed by submitting a certificate by simple KS F 2296 performance test. At present, it is developed as a simple test certification by producing a real scale fireproof door without the theoretical examination in advance, so that a high cost for improvement is occurring in Korea. Therefore, through this study, structural analysis study which can preliminary structure review was carried out in order to design the refuge connection passage fire door and to improve the performance improvement. In order to secure the reliability of the result value, the official authentication test (KS F 2296) were compared.