• Molecules and Cells
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• v.39 no.5
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• pp.375-381
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• 2016

MicroRNAs (miRNAs) are small non-coding RNAs (~22 nucleotides) regulating gene expression at the post-transcriptional level. By directing the RNA-induced silencing complex (RISC) to bind specific target mRNAs, miRNA can repress target genes and affect various biological phenotypes. Functional miRNA target recognition is known to majorly attribute specificity to consecutive pairing with seed region (position 2-8) of miRNA. Recent advances in a transcriptome-wide method of mapping miRNA binding sites (Ago HITS-CLIP) elucidated that a large portion of miRNA-target interactions in vivo are mediated not only through the canonical "seed sites" but also via non-canonical sites (~15-80%), setting the stage to expand and determine their properties. Here we focus on recent findings from transcriptome-wide non-canonical miRNA-target interactions, specifically regarding "nucleation bulges" and "seed-like motifs". We also discuss insights from Ago HITS-CLIP data alongside structural and biochemical studies, which highlight putative mechanisms of miRNA target recognition, and the biological significance of these non-canonical sites mediating marginal repression.

• BMB Reports
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• v.49 no.12
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• pp.653-654
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• 2016

The human reference genome, maintained by the Genome Reference Consortium, is conceivably the most complete genome assembly ever, since its first construction. It has continually been improved by incorporating corrections made to the previous assemblies, thanks to various technological advances. Many currently-ongoing population sequencing projects have been based on this reference genome, heightening hopes of the development of useful medical applications of genomic information, thanks to the recent maturation of high-throughput sequencing technologies. However, just one reference genome does not fit all the populations across the globe, because of the large diversity in genomic structures and technical limitations inherent to short read sequencing methods. The recent success in de novo construction of the highly contiguous Asian diploid genome AK1, by combining single molecule technologies with routine sequencing data without resorting to traditional clone-by-clone sequencing and physical mapping, reveals the nature of genomic structure variation by detecting thousands of novel structural variations and by finally filling in some of the prior gaps which had persistently remained in the current human reference genome. Now it is expected that the AK1 genome, soon to be paired with more upcoming de novo assembled genomes, will provide a chance to explore what it is really like to use ancestry-specific reference genomes instead of hg19/hg38 for population genomics. This is a major step towards the furthering of genetically-based precision medicine.

• Korean Journal of Materials Research
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• v.28 no.10
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• pp.595-600
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• 2018

This study investigates the microstructural properties of CoCrFeMnNi high entropy alloy (HEA) oxynitride thin film. The HEA oxynitride thin film is grown by the magnetron sputtering method using nitrogen and oxygen gases. The grown CoCrFeMnNi HEA film shows a microstructure with nanocrystalline regions of 5~20 nm in the amorphous region, which is confirmed by high-resolution transmission electron microscopy (HR-TEM). From the TEM electron diffraction pattern analysis crystal structure is determined to be a face centered cubic (FCC) structure with a lattice constant of 0.491 nm, which is larger than that of CoCrFeMnNi HEA. The HEA oxynitride film shows a single phase in which constituting elements are distributed homogeneously as confirmed by element mapping using a Cs-corrected scanning TEM (STEM). Mechanical properties of the CoCrFeMnNi HEA oxynitride thin film are addressed by a nano indentation method, and a hardness of 8.13 GPa and a Young's modulus of 157.3 GPa are obtained. The observed high hardness value is thought to be the result of hardening due to the nanocrystalline microstructure.

• Asia pacific journal of information systems
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• v.20 no.4
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• pp.25-51
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• 2010

This study compares and contrasts the intellectual development of the MIS field in Korea from 2005 to 2009 to that of international trends by using a keyword co-occurrence network analysis of the two flagship journals: APJIS and MIS Quarterly. From 316 research articles in these two journals, 132 unique and most frequently co-occurred keywords were put into analysis. The results of structural equivalence show a mild correlation between APJIS and MIS Quarterly. The e-commerce, trust, and technology adoption are the high frequency keywords in both journals. In Korea e-learning, purchasing, and recommendation systems turn out to be important keywords while outsourcing, research method, quantitative method, design research, information theory, and empirical research are in average international journals. This connotes that the Korean scholarship tends to focus more on practically oriented topics, but the clustering and relational mapping of research topics in each journal show a mild level of overlap with distinctive orientations due to intrinsic disparities depending on the concerned journals' geographical scopes, namely domestic or global.

• Economic and Environmental Geology
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• v.42 no.6
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• pp.627-634
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• 2009

Redefining the difference among the Yamaguchi's 'so-called Yeoncheon system,' north Korean's 'Rimjin System' and Yeoncheon Group of national geological map, authors tried to avoid a possible misunderstanding of concept between Yeoncheon Group and Imjingang fold Belt. As a tool of preliminary geological mapping and or N-S reconnaissance of the whole Yeoncheon Group, an interpretation technique of satellite image from various angles has been applied to understand the geometrical attitude of strike and dip of random spot, and a further concept like as cross section.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.512.1-512
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• 1986

The cdd gene of Bacillus subtilis, encoding the deoxycytidinecytidine deaminase of pyrimidine nucleotide biosynthesis has been cloned into the EcoRl site of pBR322. The recombinant plasmid, pSol, promoted the synthesis of 100-140 fold elevated levels of the enzyme. A comparison of the polypeptides encoded by cdd complementing and non-complementing plasmids in the mini cell showed the gene product to have a molecular mass of approximately 14 kDa. The nucleotide sequence of the gene and 460 base pairs upstream from the coding region was determined. An open-reading frame, encoding a protein with a calculated molecular mass of 14337 Da, was deduced to be the coding region for cdd. However, the enzyme has an apparent molecular mass of 54 kDa as determined by gel filteration, whereas sucrose density gradient centrifugation shows 58 kDa. It means that the enzyme could be forming a tetramer in a physiological state. About 28 amino acids of the N-tetramer in a physiological state. About 28 amino acids of the N-terminal presumably form a signal for membrane translocation and six cystein residues are contained in the structure. S1 nuclease mapping indicated that transcription of cdd is initiated 17 base pairs upstream from the translational start. The structural characterization of the odd gene was performed.

• Journal of the Korean Society of Industry Convergence
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• v.23 no.3
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• pp.463-471
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• 2020

Recently, various tries to apply ROV (Remotely Operated Vehicle) into underwater are being developed. However, due to underwater environment uniqueness, the additional problem must be taken into account when designing an ROV for the inspection of the underwater structure. This is because a GPS-based information method cannot be applied, and the obtainable image is also dependent on the turbidity. Also, it is necessary to be able to satisfy waterproof and operating speeds in consideration of most practical application environments. This paper describes the design results of the ROV system for underwater structure inspection considering the above problems. The designed system applied INS / DVL for location recognition and was configured to support 3D mapping and stereo camera-based image information using sonar depending on visibility. To satisfy the waterproof, a pressure vessel using a composite material was applied. And over-actuated system using eight thrusters to maintain a stable posture and operating speed was applied also. The designed system was verified by structural analysis and flow analysis also.

• Smart Structures and Systems
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• v.8 no.1
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• pp.69-92
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• 2011

This paper presents an emergent pattern recognition approach based on the immune network theory and hierarchical clustering algorithms. The immune network allows its components to change and learn patterns by changing the strength of connections between individual components. The presented immune-network-based approach achieves emergent pattern recognition by dynamically generating an internal image for the input data patterns. The members (feature vectors for each data pattern) of the internal image are produced by an immune network model to form a network of antibody memory cells. To classify antibody memory cells to different data patterns, hierarchical clustering algorithms are used to create an antibody memory cell clustering. In addition, evaluation graphs and L method are used to determine the best number of clusters for the antibody memory cell clustering. The presented immune-network-based emergent pattern recognition (INEPR) algorithm can automatically generate an internal image mapping to the input data patterns without the need of specifying the number of patterns in advance. The INEPR algorithm has been tested using a benchmark civil structure. The test results show that the INEPR algorithm is able to recognize new structural damage patterns.

• Structural Engineering and Mechanics
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• v.25 no.2
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• pp.161-180
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• 2007

A first endeavor is made to exploit the differential quadrature method (DQM) as a simple, accurate, and computationally efficient numerical tool for the large deformation analysis of thin laminated composite skew plates, which has very strong singularity at the obtuse vertex. The geometrical nonlinearity is modeled by using Green's strain and von Karman assumption. A recently developed DQ methodology is used to exactly implement the multiple boundary conditions at the edges of skew plates, which is a major draw back of conventional DQM. Using oblique coordinate system and the DQ methodology, a mapping-DQ discretization rule is developed to simultaneously transform and discretize the equilibrium equations and the related boundary conditions. The effects of skew angle, aspect ratio and different types of boundary conditions on the convergence and accuracy of the presented method are studied. Comparing the results with the available results from other numerical or analytical methods, it is shown that accurate results are obtained even when using only small number of grid points. Finally, numerical results for large deflection behavior of antisymmetric cross ply skew plates with different geometrical parameters and boundary conditions are presented.

• Journal of Ship and Ocean Technology
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• v.10 no.2
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• pp.11-23
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• 2006

The model exchange from CAD system to CAE system in valid and effective manner is the major issue of automatic analysis modelling of ship structure. However, model exchange approaches based on the neutral CAD file have resulted in invalid model exchange that could not properly reflect the characteristics of CAD model and CAE model of ship structure. This paper presents the new approach of n-to-n mapping to exchange ship structure model in heterogeneous CAD/CAE environments. In this study, the common model called 'unified ship model for analysis' to directly extract proper information from different CAD systems for ship structural analysis is proposed. Moreover, a command language based model interfacing technique to construct an idealized model for analysis job is also proposed. The proposed approach has been actually implemented in DSME CAD/CAE environment of ship structure such as TRIBON system, PATRAN system and FLUENT system. The applicability and effectiveness of the proposed approach was verified by applying it to the real analysis project for fore-body of ship and block lifting. This application results show that the proposed approach can be effectively used for heterogeneous CAD/CAE environment.