• Journal of Microbiology
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• 제34권1호
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• pp.74-81
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• 1996

Two secretion vectors, pUBA240 and pUB340 were constructed by using the promoter and secretory signal region of the .alpha.-amylase gene from an .alpha.-amylase hyperproducing strain, Bacillus subtilis NA64. In this secretion vector system, various restriction enzyme sites are located immediately after the proregion of the .alpha.-amylase gene for easy replacement of various foregn structural genes. To evaluate this secretion vectors, the .betha.-lactamase gene of pBR322 was used as a reporter gene. The expressed and biologically active .betha.-lactamase was secreted into the culture broth from B. subtilis LKS86 transformants harboring each .betha.-lactamase secreting plasmid, pUBAbla and pUBSble. In both cases, more than 92% of expressed .betha.- lactamases were located idn the culture medium. The amount of the secreted .betha.-lactamase was about 80% of the total secreted proteins in the culture medium.

• Journal of Life Science
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• 제9권1호
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• pp.5-8
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• 1999

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a major virulence factor and a potent immunogen. Deletion mutageneses were performed in the H. pylori urease accessory genes by using combinations of restriction enzymes and other DNA modifying enzymes in order to assess the function of these accessory gene products in the expression of the active urease. Selective disruptions in the accessory gene regions resulted in complete abolishment of the urease activity, which is consistent with other bacterial ureases. Interestingly, deletions in ureE-containing regions caused reduced expression of the structural enzyme subunits.

• 아동학회지
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• 제31권4호
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• pp.125-137
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• 2010

This study examined the pathways from maternal parenting behavior to children's school adjustment through children's emotional intelligence. The participants in this study were 436 elementary school 4th-6th graders (of which 233 were boys, and 203 were girls) in Seoul. They completed questionnaires on maternal parenting behavior, children's emotional intelligence, and levels of school adjustment. Data were analyzed by means of Pearson's correlation coefficients and structural equation modeling. It was found that maternal parenting behavior indirectly, but not directly, influenced children's levels of school adjustment through children's emotional intelligence. Children's emotional intelligence mediated the effects of maternal warmth/acceptance and rejection/restriction on levels of children's school adjustment. These results clearly indicate that emotional intelligence plays a crucial role in improving the levels of children's school adjustment.

• Structural Engineering and Mechanics
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• 제66권1호
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• pp.27-36
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• 2018

The objective of this work is to analyze geometrically nonlinear static analysis a simply supported laminated composite beam subjected to a non-follower transversal point load at the midpoint of the beam. In the nonlinear model of the laminated beam, total Lagrangian finite element model of is used in conjunction with the Timoshenko beam theory. The considered non-linear problem is solved considering full geometric non-linearity by using incremental displacement-based finite element method in conjunction with Newton-Raphson iteration method. There is no restriction on the magnitudes of deflections and rotations in contradistinction to von-Karman strain displacement relations of the beam. In the numerical results, the effects of the fiber orientation angles and the stacking sequence of laminates on the nonlinear deflections and stresses of the composite laminated beam are examined and discussed. Convergence study is performed. Also, the difference between the geometrically linear and nonlinear analysis of laminated beam is investigated in detail.

• Structural Engineering and Mechanics
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• 제17권6호
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• pp.829-862
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• 2004

This paper presents a method for the nonlinear analysis of beam elements subjected to the cyclical combined actions of torsion, biaxial flexure and axial forces based on an extension of the disturbed compression field (DSFM). The theoretical model is based on a hybrid formulation between the full rotation of the cracks model and the fixed direction of the cracking model. The described formulation, which treats cracked concrete as an orthotropic material, includes a new approach for the evaluation of the re-orientation of both the compression field and the deformation field by removing the restriction of their coincidence. A new equation of congruence permits evaluating the deformation of the middle line. The problem consists in the solution of coupled nonlinear simultaneous equations expressing equilibrium, congruence and the constitutive laws. The proposed method makes it possible to determine the deformations of the beam element according to the external stresses applied.

• Molecules and Cells
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• 제44권7호
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• pp.425-432
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• 2021

Aging is associated with functional and structural declines in organisms over time. Organisms as diverse as the nematode Caenorhabditis elegans and mammals share signaling pathways that regulate aging and lifespan. In this review, we discuss recent combinatorial approach to aging research employing C. elegans and mammalian systems that have contributed to our understanding of evolutionarily conserved aging-regulating pathways. The topics covered here include insulin/IGF-1, mechanistic target of rapamycin (mTOR), and sirtuin signaling pathways; dietary restriction; autophagy; mitochondria; and the nervous system. A combinatorial approach employing high-throughput, rapid C. elegans systems, and human model mammalian systems is likely to continue providing mechanistic insights into aging biology and will help develop therapeutics against age-associated disorders.

• 가정과삶의질연구
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• 제23권4호
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• pp.35-53
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• 2005

In this study, the influence of mothers' parenting behaviors on children's strategies and competence of emotional regulation was examined. Further, the mediating effects of children's active-social support seeking and aggressive strategies on the above relationship were explored. The participants were W mother-child pairs. The children were 5th and 6th graders at two elementary schools in Kyunggi province and Kwangju metropolitan area The data were analyzed with descriptive statistics, factor analysis, Cronbach's alpha, Pearson correlations, standard multiple regressions and structural equation modeling analysis by LISREL 8.3. The main results of this study were as follows: (1) The more the mothers coached children with affection and reasoning, the more adaptive emotional regulation the children had; whereas children tended to have maladaptive emotional regulation in response to the mothers' rejecting and forceful parenting behaviors. Also, when children were coached by mothers with love, reasoning and consistent restriction, they used more active-social support seeking strategies, whereas they used more aggressive strategies when the mothers coached children with rejecting and forceful parenting behaviors. The more the mothers were rejecting, forceful and intervening, the more the children used passive-avoidant strategies. (2) The more the children used active-social support seeking strategies and the less the children used aggressive strategies, the more likely they had adaptive emotional regulation. The more the children used aggressive strategies, the more likely they had maladaptive emotional regulation. (3) Children's active-social support seeking strategies played a partial mediating role between mothers' affectionate and reasoned coaching and children's adaptive emotional regulation. These strategies, on the other hand, played a full mediating role between mothers' consistent restriction and children's adaptive emotional regulation. Children's aggressive strategies played a partial mediating role between mothers' rejecting and forceful parenting behaviors and children's maladaptive emotional regulation. Mothers' non-intervention had an influence on neither the children's aggressive strategies nor their maladaptive emotional regulation.

• 미생물학회지
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• 제24권4호
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• pp.341-351
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• 1986

항생제에 대하여 다중 저항성을 갖는 Shaphylococcus aµreus D-H-1으로부터 chloram phenicol(Cm) 저항성 유전자를 가진것으로 확인된 R-plasmid(pSBK203, 2.5MdaJ.)를 분리하여 Bacillus subtilis BD 170 내에셔 발현시켰으며 이 Cm 저항성 유전자를 cloning하기 위하여 pSBK203상의 제한효소 인식부위를 결정하였다. pSBK203 상의 TaqI 부분 절편 0.3 kb을 pBD9 CZaI 인식부위내에 삽입하여 얻은 재조합 plasmid(pTQ16)와 TaqI부 분절편과 O.lkb 엇갈럼이 있는 RsaI 단일절떤(1. 3 kb} pBR322의 Seal 인식부위에 삽입하여 얻은 재조합 p plasmid(pHW20)에서 Cm 저항성이 획득되었다. pBD9 및 pBR322 상에 삽입된 두 절펀내에 Hinf, Taq I 빛 BglII의 제한효소 인식부위가 존재하였으며 이들 중 행III 인식부위에 의하여 Cm 저항성이 불활성 되었다.

• 대한수의학회지
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• 제39권4호
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• pp.797-805
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• 1999

T sergenti cDNA library were constructed to get a more broad information about the structural, functional or antigenic properties of the proteins, and analyzes for their partial cDNA sequences and expression sequences tags(ESTg). The mRNA were purified from T sergenti isolates to identify the information of antigen gene, then first and second strand cDNA was synthesized. EcoR I adaptor ligation and Xho I enzyme restriction were used to the synthesized cDNA, and ligated into a Uni-ZAP XR vector. T sergenti cDNA library was constructed with packaging and amplification in vitro. Antibody screening was performed with constructed T sergenti cDNA library using antisera against T sergenti. Among those clones, eight phagemids were rescued from the recombinant in vivo excision with f1 helper phage. Using the analysis of endonuclease restriction and PCR, the recombinant cDNA were proved having a 0.5-3.0kb of inserts. The eight of partial cDNA clones' sequences were obtained and examined for their homology using BLASTN and BLASTX. The eight of sequenced clones were classified into three groups according to the basis of database searches. A total 3,045bp of partial cDNA sequence were determined from six clones. The putatively identified clones contain a cytochrome c gene, a heat shock protein gene, a cyclophilin gene, and a ribosomal protein gene. The unidentified clones have a homology to ATP-binding protein(mtrA) gene of S argillaceus, DNA-binding protein(DBP) gene of Pseudorabies virus 85kDa merozoite protein gene of B bovis, mRNA spm1 protein of T annulata and glycine-rich RNA-binding protein mRNA of O sativa etc.

• 재활치료과학
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• 제7권4호
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• pp.19-30
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• 2018

목적 : 본 연구의 목적은 지역사회 거주 척수장애인의 참여 제약과 관련이 있는 요인간의 상관관계 및 그 영향을 종합적으로 분석하는 것이다. 연구방법 : 연구 대상은 지역사회에 거주하는 미국 척수 손상 협회(American Spinal Injury Assocation; ASIA) 분류법 상 손상정도(Frankel type)가 A,B,C에 해당하는 만 18세 이상 성인 척수장애인 82명이었다. 대상자들을 통하여 참여, 환경적 요인, 기능적 요인, 손상관련 요인, 심리사회적 요인, 인구사회학적 요인을 조사하였다. 참여 수준은 한국형 단축형 크레이그 핸디캡 평가(CHART-K-SF)로, 환경적 요인은 CHIEF-25 한국형으로, 기능적 요인은 척수손상환자 독립성 지수SCIM-III을 사용하였다. 손상관련 요인의 통증 및 통증관련 심리적 고통은 Pain and Distress Scale (PAD)로, 심리사회적 요인 중 자존감은 로젠버그 자아존중감 척도(RSES), 자기효능감은 일반적 자기효능감 척도(GSS)로 평가하였다. 수집된 정보는 SPSS Version 21.0을 통하여 상관관계분석, 로지스틱 회귀분석, 다중회귀분석에 사용되었다. 결과 : 참여 제약의 위험요인은 환경적 요인과 기능적 요인이었으며, 본 회귀모형의 설명력은 약 73.7%였다(R2N=.737). 환경적 장벽이 있는 것이 없는 것에 비해 약 40배 정도 참여 제약을 겪을 위험도가 높았으며, 기능적 수준이 4사분위수(상위 25%)인 것에 비해, 2사분위수일 경우 약 21배, 1사분위수(하위25%)일 경우 약 236배 정도 참여 제약을 겪을 위험도가 높아졌다. 결론 : 본 연구는 지역사회 거주 척수장애인의 참여 제약의 예측요인을 다각적인 측면으로 확인한 것에 의의가 있으며, 이를 통하여 지역사회에서 척수장애인에 대한 작업치료사의 역할을 규명한 것에 중요한 의미를 갖는다.